##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933932.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2387511
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.416463421529784	33.0	31.0	34.0	31.0	34.0
2	32.671519838023784	34.0	31.0	34.0	31.0	34.0
3	32.65808157533096	34.0	31.0	34.0	31.0	34.0
4	36.092007115359884	37.0	35.0	37.0	35.0	37.0
5	36.14663262284446	37.0	35.0	37.0	35.0	37.0
6	35.811350397966756	37.0	35.0	37.0	35.0	37.0
7	36.12124425814164	37.0	35.0	37.0	35.0	37.0
8	36.13532335557826	37.0	37.0	37.0	35.0	37.0
9	38.001760410737376	39.0	39.0	39.0	37.0	39.0
10	37.6700764938884	39.0	37.0	39.0	35.0	39.0
11	37.60908033512725	39.0	37.0	39.0	35.0	39.0
12	37.47114170364032	39.0	37.0	39.0	35.0	39.0
13	37.43195989463504	39.0	37.0	39.0	35.0	39.0
14	38.594670768009024	40.0	38.0	41.0	34.0	41.0
15	38.66630394582475	40.0	38.0	41.0	34.0	41.0
16	38.74161082399201	40.0	38.0	41.0	35.0	41.0
17	38.73021485555459	40.0	38.0	41.0	35.0	41.0
18	38.59528186466994	40.0	38.0	41.0	35.0	41.0
19	38.51743677830176	40.0	37.0	41.0	35.0	41.0
20	38.37098677241696	40.0	37.0	41.0	34.0	41.0
21	38.28217168423517	40.0	37.0	41.0	34.0	41.0
22	38.3111868385109	40.0	37.0	41.0	34.0	41.0
23	38.261017436150034	40.0	37.0	41.0	34.0	41.0
24	38.21593073288458	40.0	37.0	41.0	34.0	41.0
25	38.117125324239346	40.0	36.0	41.0	34.0	41.0
26	38.04639182814236	40.0	36.0	41.0	34.0	41.0
27	38.0282930633618	40.0	36.0	41.0	34.0	41.0
28	37.94786704647643	40.0	36.0	41.0	34.0	41.0
29	37.8879221917721	40.0	36.0	41.0	34.0	41.0
30	37.75558060256058	40.0	36.0	41.0	33.0	41.0
31	37.626035649678684	40.0	36.0	41.0	33.0	41.0
32	37.42456265122967	40.0	35.0	41.0	33.0	41.0
33	37.192946964432835	40.0	35.0	41.0	32.0	41.0
34	36.98299777466994	40.0	35.0	41.0	31.0	41.0
35	36.83146130007359	40.0	35.0	41.0	31.0	41.0
36	36.68819578213461	39.0	35.0	41.0	31.0	41.0
37	36.612565135825555	39.0	35.0	41.0	31.0	41.0
38	36.561088514356584	39.0	35.0	41.0	30.0	41.0
39	36.49969864013192	39.0	35.0	41.0	30.0	41.0
40	36.38199405154573	39.0	35.0	41.0	30.0	41.0
41	36.25504845841548	39.0	35.0	41.0	30.0	41.0
42	36.206296431723246	39.0	35.0	41.0	30.0	41.0
43	36.08765614064186	39.0	35.0	41.0	30.0	41.0
44	35.979057269264935	39.0	35.0	41.0	29.0	41.0
45	36.00232124584976	39.0	35.0	41.0	29.0	41.0
46	35.9577178911427	39.0	35.0	41.0	29.0	41.0
47	35.90147856910397	39.0	35.0	41.0	29.0	41.0
48	35.82966654394472	39.0	35.0	41.0	28.0	41.0
49	35.81407247966606	39.0	35.0	40.0	28.0	41.0
50	35.73152207466269	39.0	35.0	40.0	28.0	41.0
51	34.81877905483996	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	27.0
10	55.0
11	44.0
12	31.0
13	46.0
14	39.0
15	64.0
16	107.0
17	205.0
18	364.0
19	742.0
20	1323.0
21	2228.0
22	3530.0
23	5851.0
24	10214.0
25	18045.0
26	27540.0
27	31665.0
28	31797.0
29	30731.0
30	33254.0
31	38981.0
32	50232.0
33	71497.0
34	130536.0
35	152715.0
36	170891.0
37	263890.0
38	511609.0
39	798721.0
40	527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.812260131995203	4.150054177760857	52.699778137147845	22.33790755309609
2	23.132458866158103	4.208776420297121	51.695803705197584	20.962961008347186
3	18.080377430721786	4.534596908663458	55.76376402035426	21.621261640260506
4	17.54446366948676	4.511309057843084	54.79966375024031	23.144563522429845
5	17.81654618554637	5.301504370032222	53.481177678343684	23.400771766077728
6	20.325770226817802	5.9548207317160005	55.298635273303454	18.420773768162743
7	78.76269470590921	1.775866163548566	14.20864657796341	5.252792552578815
8	80.74224579488849	2.2355499095082703	12.307126543081896	4.715077752521349
9	75.24941246343995	4.404838344200298	14.570990458263857	5.774758734095885
10	35.3012823815262	29.431236128336163	20.905662843019364	14.361818647118273
11	25.802980593597262	22.841444500150995	31.688859234575258	19.666715671676485
12	23.001150570615174	20.972845779558714	34.53001891928456	21.495984730541558
13	22.611916761849475	20.39776989509158	37.02324303427293	19.96707030878601
14	19.01448831021093	22.98871083735321	36.79120221854475	21.20559863389111
15	17.266810498464718	23.724874984869178	38.31358263899099	20.694731877675117
16	20.561580658685973	24.36160503553701	34.47862648590939	20.598187819867636
17	21.287189881009972	24.077585401700766	33.14091537169881	21.494309345590448
18	21.252341874026968	23.11637517062749	34.235737552622794	21.395545402722753
19	21.780381325991797	25.229286901714797	32.29983024161983	20.69050153067358
20	22.09036942657018	26.734955357273748	31.808314181589108	19.36636103456696
21	22.08777257989597	25.776509511369788	33.40177280858601	18.73394510014823
22	21.00513882449128	23.233191386343353	33.71284990938261	22.04881987978275
23	20.152493538249665	24.11151194696066	33.093585746830065	22.642408767959605
24	20.461685830976275	24.154778763322977	32.546279367927525	22.83725603777323
25	21.144405198551965	25.524447845475894	30.35391250553401	22.977234450438132
26	19.610045775705327	26.498139694434915	30.669973876560153	23.22184065329961
27	19.421439314834572	25.386438010128536	32.52567213302891	22.666450542007972
28	18.29574816618646	25.015298358834787	32.22783057334605	24.461122901632702
29	19.98403358141596	25.175842121774515	31.739581513969988	23.100542782839533
30	21.36421570413707	24.929392995466827	32.07759042785562	21.62880087254048
31	20.565769121063735	26.128968620458714	31.10854777213592	22.196714486341634
32	22.431687225734247	26.085157303987287	29.579298273390155	21.903857196888307
33	23.119767825153474	25.013958050873903	29.912574224788912	21.95369989918371
34	20.623904978867113	24.72172903077724	32.097988239635335	22.556377750720312
35	21.27793337915511	26.343627317319168	30.013348629597935	22.365090673927785
36	22.670722773633294	26.91417966241831	29.402461391800916	21.012636172147477
37	21.918893776824483	26.694369156833204	30.181766701807867	21.204970364534447
38	23.267034162355692	24.5025467945488	30.13640565425667	22.09401338883884
39	23.0723125464134	22.921569785437637	31.459666573263956	22.546451094885008
40	23.093966896906444	22.82196815009439	33.06334504846261	21.020719904536566
41	22.156463362891312	24.129647989056384	30.75407820110567	22.959810446946634
42	23.277672856795213	24.375008115145857	29.965097543006085	22.382221485052845
43	22.231939454938637	24.099868021550478	30.56865497164202	23.09953755186887
44	21.698790078872936	23.721231022600524	29.894019336455415	24.685959562071126
45	20.92229103865909	23.358468296062302	29.663779559549674	26.055461105728938
46	21.226582830403714	24.133375720572598	30.60496894045724	24.03507250856645
47	19.667595248775818	24.93345580397326	32.18510825709285	23.213840690158076
48	20.212556088746815	24.30598225516029	31.604042871425513	23.877418784667377
49	22.102055236604144	22.364671827690007	32.11013478053085	23.423138155174993
50	20.806144976923665	22.623686341130995	31.734639128364222	24.835529553581114
51	20.0980854119625	22.053929803883626	30.880485995666618	26.96749878848726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	488.0
1	1524.0
2	2560.0
3	23429.5
4	44299.0
5	29035.5
6	13772.0
7	13671.0
8	13570.0
9	13616.0
10	13662.0
11	13304.0
12	12946.0
13	12383.0
14	11820.0
15	11424.0
16	11028.0
17	10497.0
18	9966.0
19	9647.5
20	9329.0
21	9920.0
22	10511.0
23	11309.5
24	12108.0
25	13855.5
26	18567.0
27	21531.0
28	25905.0
29	30279.0
30	34932.5
31	39586.0
32	45846.0
33	52106.0
34	57713.5
35	63321.0
36	69251.0
37	75181.0
38	81825.5
39	88470.0
40	96488.5
41	104507.0
42	115363.0
43	126219.0
44	142094.5
45	157970.0
46	191496.0
47	225022.0
48	249299.5
49	273577.0
50	264017.5
51	254458.0
52	213239.5
53	172021.0
54	148787.0
55	125553.0
56	113129.0
57	100705.0
58	90470.0
59	80235.0
60	71333.5
61	62432.0
62	55323.5
63	48215.0
64	41277.0
65	34339.0
66	29992.5
67	25646.0
68	21143.0
69	16640.0
70	14131.0
71	11622.0
72	9448.0
73	7274.0
74	5911.0
75	3338.5
76	2129.0
77	1713.5
78	1298.0
79	939.5
80	581.0
81	404.5
82	228.0
83	153.0
84	78.0
85	59.5
86	41.0
87	27.5
88	14.0
89	16.5
90	19.0
91	11.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2387511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.77149895267942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33784621744694	23.37848152601468
2	11.463342785105452	7.513418521525771
3	4.301351977882971	4.2288525551489204
4	2.193612218642188	2.8755184210326896
5	1.4134900499995207	2.3161093846591037
6	1.0077691123744368	1.9815662646432919
7	0.7811790256116463	1.7920285333781039
8	0.6439967140825118	1.6883790112867219
9	0.5592470165252557	1.6494626714711857
>10	6.082941189426307	40.02161531968279
>50	0.15013004206216193	3.240753690147942
>100	0.05774809116119307	3.522857450348154
>500	0.004510431382085313	0.9988820581925909
>1k	0.0019330420208937046	1.5398375919059717
>5k	6.443473402979015E-4	1.6150535967106818
>10k+	2.577389361191606E-4	1.6371834038514277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27628	1.1571883857289034	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	11138	0.46651093963546136	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	9587	0.4015478881563268	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	8157	0.3416528761542879	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	7381	0.3091504081028318	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	7364	0.30843836949861175	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	5753	0.24096224059281823	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	4988	0.20892050340291626	No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	4977	0.2084597725413621	No Hit
CGTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	4923	0.20619800285736906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC	3185	0.133402526731814	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC	2800	0.11727694657741891	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	2480	0.10387386696857102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.931923664435473E-4	0.0	0.0	0.4183436222911643	0.0
2	2.931923664435473E-4	0.0	0.0	1.3608733111596134	0.0
3	2.931923664435473E-4	0.0	0.0	2.1413513906323365	0.0
4	3.350769902211969E-4	0.0	0.0	2.9389183966063404	0.0
5	3.350769902211969E-4	0.0	0.0	4.37237776077262	0.0
6	3.350769902211969E-4	0.0	0.0	5.879721601282674	0.0
7	3.350769902211969E-4	0.0	0.0	7.112846809920457	0.0
8	3.350769902211969E-4	0.0	0.0	8.710326360800012	0.0
9	3.350769902211969E-4	0.0	0.0	9.713169907908277	0.0
10	3.350769902211969E-4	0.0	0.0	11.076975142732326	0.0
11	3.350769902211969E-4	0.0	0.0	13.037175535526329	0.0
12	3.350769902211969E-4	0.0	0.0	14.753439879439298	0.0
13	3.350769902211969E-4	0.0	0.0	15.400724855299096	0.0
14	3.350769902211969E-4	0.0	0.0	15.711927609967033	0.0
15	3.350769902211969E-4	0.0	0.0	16.031423520142944	0.0
16	3.350769902211969E-4	0.0	0.0	16.610017713007394	0.0
17	3.350769902211969E-4	0.0	0.0	17.41416060491449	0.0
18	3.350769902211969E-4	0.0	0.0	18.278910547427845	0.0
19	3.350769902211969E-4	0.0	0.0	18.9678707239464	0.0
20	3.350769902211969E-4	0.0	0.0	19.712034834603905	0.0
21	3.769616139988465E-4	0.0	0.0	20.701014571241767	0.0
22	3.769616139988465E-4	0.0	0.0	21.79474775194753	0.0
23	3.769616139988465E-4	0.0	0.0	22.815894879646628	0.0
24	4.188462377764961E-4	0.0	0.0	23.61559800143329	0.0
25	4.188462377764961E-4	0.0	0.0	24.313856564430488	0.0
26	4.188462377764961E-4	0.0	0.0	24.95376984650542	0.0
27	4.188462377764961E-4	0.0	0.0	25.615086171330727	0.0
28	4.188462377764961E-4	0.0	0.0	26.28084226627647	0.0
29	4.188462377764961E-4	0.0	0.0	26.99061910081252	0.0
30	4.188462377764961E-4	0.0	0.0	27.759369485627502	0.0
31	4.188462377764961E-4	0.0	0.0	28.46629816574667	0.0
32	4.188462377764961E-4	0.0	0.0	29.146755763638367	0.0
33	4.188462377764961E-4	0.0	0.0	29.81799874429898	0.0
34	4.188462377764961E-4	0.0	0.0	30.504110766400657	0.0
35	4.188462377764961E-4	0.0	0.0	31.228840411625328	0.0
36	4.188462377764961E-4	0.0	0.0	31.890240505698195	0.0
37	4.188462377764961E-4	0.0	0.0	32.58276087523785	0.0
38	4.188462377764961E-4	0.0	0.0	33.31867371501116	0.0
39	4.188462377764961E-4	0.0	0.0	34.387485544569216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACTA	20	7.034815E-4	45.0	13
CGTTTTT	14855	0.0	43.985188	1
CGTTTCT	1040	0.0	38.509613	1
CGTTTTC	755	0.0	38.145695	1
CCATACG	160	0.0	37.96875	2
TGGGCGA	2965	0.0	37.71501	6
TTTGGGA	13670	0.0	37.52743	4
TTGGGAC	5610	0.0	37.41979	5
GTTTTTT	17895	0.0	37.091366	2
TTGGGAT	8115	0.0	36.98706	5
TTTCTCG	1060	0.0	36.933964	1
GCGATCG	105	0.0	36.428574	9
CGTTCTG	955	0.0	36.047123	1
ATAGGCG	25	0.0021075623	36.0	13
AATCGTT	75	1.8189894E-12	36.0	22
TAGCGGG	470	0.0	35.904255	3
TGCGGGA	3355	0.0	35.812218	4
GGGCGAT	4870	0.0	35.805954	7
TCTTGCG	850	0.0	35.735294	1
TTTGGGC	4800	0.0	35.671875	4
>>END_MODULE