FastQCFastQC Report
Sat 14 Jan 2017
SRR2933931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596942
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC100110.626885635170219TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT89230.5587554212989576TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC79440.4974507527511957TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC63210.3958190090811063TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.24058481773289198No Hit
TCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG33990.21284429866582508No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG30530.19117788873985406No Hit
TTCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT21210.13281634523983962No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG18490.11578379177202427TruSeq Adapter, Index 20 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG18180.11384258163414827No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC17850.11177613213253831No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG253.891491E-545.02
TAACCGC207.0341135E-445.017
CCGCCGT253.891491E-545.036
ACGTTAT603.6379788E-1241.24999624
CGTTTTT20400.041.029411
TACGGGA2950.040.423734
TTTGGGA115300.039.3018234
TTGGGAC51000.038.8235325
TTGGGAT70350.038.699365
CTAAACG356.2498184E-638.57142645
GCGCGAC2600.038.076929
TTATCCG659.094947E-1238.076921
GCGATTC4800.037.9687469
TTTGGGC34300.037.784264
TTTTCCG10050.037.611941
TGGGCGA21000.037.6071436
CAACCCG900.037.523
CGCGGTA601.5643309E-1037.49999631
GCGATCG301.1401211E-437.4999969
ACCGTAC301.1401211E-437.49999622
Produced by FastQC (version 0.11.5)