##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933931.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1596942
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43159112854443	33.0	31.0	34.0	31.0	34.0
2	32.66094635872812	34.0	31.0	34.0	31.0	34.0
3	32.71420439815598	34.0	31.0	34.0	31.0	34.0
4	36.08620788982944	37.0	35.0	37.0	35.0	37.0
5	36.1303697942693	37.0	35.0	37.0	35.0	37.0
6	35.81386737902817	37.0	35.0	37.0	35.0	37.0
7	36.126478607238084	37.0	35.0	37.0	35.0	37.0
8	36.14093811797799	37.0	37.0	37.0	35.0	37.0
9	38.005110392237164	39.0	39.0	39.0	37.0	39.0
10	37.638146532560356	39.0	37.0	39.0	35.0	39.0
11	37.56490530025511	39.0	37.0	39.0	35.0	39.0
12	37.35309422634009	39.0	37.0	39.0	35.0	39.0
13	37.241754553390166	39.0	37.0	39.0	34.0	39.0
14	38.13303113074864	40.0	38.0	41.0	33.0	41.0
15	38.28928414432083	40.0	38.0	41.0	33.0	41.0
16	38.45812371394828	40.0	38.0	41.0	34.0	41.0
17	38.49605370764874	40.0	38.0	41.0	34.0	41.0
18	38.479027416149115	40.0	38.0	41.0	35.0	41.0
19	38.499122072060224	40.0	38.0	41.0	34.0	41.0
20	38.40056620716344	40.0	37.0	41.0	34.0	41.0
21	38.33119737598485	40.0	37.0	41.0	34.0	41.0
22	38.40350056545573	40.0	37.0	41.0	34.0	41.0
23	38.36401259407042	40.0	37.0	41.0	34.0	41.0
24	38.32903198738589	40.0	37.0	41.0	34.0	41.0
25	38.24022099738124	40.0	37.0	41.0	34.0	41.0
26	38.206187826483365	40.0	37.0	41.0	34.0	41.0
27	38.20793491560745	40.0	37.0	41.0	34.0	41.0
28	38.137474623373926	40.0	37.0	41.0	34.0	41.0
29	38.118043736090605	40.0	37.0	41.0	34.0	41.0
30	38.059304595908934	40.0	37.0	41.0	34.0	41.0
31	37.96016887275806	40.0	37.0	41.0	34.0	41.0
32	37.92471110409771	40.0	36.0	41.0	34.0	41.0
33	37.88963594169356	40.0	36.0	41.0	34.0	41.0
34	37.838337272111325	40.0	36.0	41.0	33.0	41.0
35	37.81104135278551	40.0	36.0	41.0	33.0	41.0
36	37.71873493213905	40.0	36.0	41.0	33.0	41.0
37	37.609310795257436	40.0	36.0	41.0	33.0	41.0
38	37.57495012342339	40.0	36.0	41.0	33.0	41.0
39	37.55721184614094	40.0	36.0	41.0	33.0	41.0
40	37.48028920273873	40.0	36.0	41.0	33.0	41.0
41	37.370194409064325	40.0	35.0	41.0	33.0	41.0
42	37.35674495379293	40.0	35.0	41.0	33.0	41.0
43	37.2539140432151	39.0	35.0	41.0	33.0	41.0
44	37.153765133611614	39.0	35.0	41.0	32.0	41.0
45	37.19420555035812	39.0	35.0	41.0	32.0	41.0
46	37.13267044138109	39.0	35.0	41.0	32.0	41.0
47	37.05777667567138	39.0	35.0	41.0	32.0	41.0
48	37.00924830081493	39.0	35.0	41.0	32.0	41.0
49	37.02810308702507	39.0	35.0	41.0	32.0	41.0
50	36.96169178342107	39.0	35.0	41.0	32.0	41.0
51	35.99475247065955	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	13.0
10	13.0
11	23.0
12	19.0
13	16.0
14	15.0
15	31.0
16	64.0
17	123.0
18	193.0
19	369.0
20	646.0
21	1013.0
22	1632.0
23	2375.0
24	3638.0
25	5336.0
26	7363.0
27	9342.0
28	10971.0
29	13494.0
30	17541.0
31	23580.0
32	32819.0
33	51838.0
34	103282.0
35	102023.0
36	105681.0
37	171107.0
38	344522.0
39	587513.0
40	343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.259882951290653	4.234155028798792	58.83519877365615	15.670763246254404
2	19.234011003530497	3.8879934274381913	55.1977466933677	21.68024887566361
3	19.571969426566525	4.087499733866353	53.90834482404495	22.432186015522166
4	18.87970884352719	4.458396109564404	52.52244602496522	24.13944902194319
5	18.0230089759052	5.127487410312961	52.263075302672235	24.58642831110961
6	20.75491783671542	5.61679760442145	54.48093919503651	19.147345363826613
7	83.31385861227271	1.5296735886463002	9.924969097186999	5.231498701893995
8	85.21323880266158	1.8360090723395088	7.5309560397309365	5.4197960852679685
9	79.64985578687266	4.777318149312874	10.079952809807745	5.49287325400672
10	37.02388690384497	33.25894115127537	16.275919851816784	13.441252093062866
11	27.609268213873765	22.793188481485238	29.720240309291135	19.87730299534986
12	26.01421967735835	20.640198579535138	32.64238776361321	20.703193979493307
13	25.29572144761676	21.654825284825623	33.65369562576474	19.395757641792876
14	19.45186487674568	23.90788143839914	35.519511666672926	21.120742018182252
15	16.9921011533293	24.314408413079498	37.622030105038256	21.071460328552945
16	22.16799357772543	24.53558112943363	31.216850705911675	22.07957458692927
17	22.626620127719104	24.872287158832318	29.14194754724968	23.359145166198896
18	23.34875029900898	23.201218328530405	31.63558851855609	21.814442853904524
19	23.141980109484255	25.526600214660274	30.154507803038555	21.176911872816923
20	22.15697251371684	29.093793011893982	29.889751788105016	18.85948268628416
21	21.981950502898666	26.68111928924156	32.88190804675436	18.455022161105415
22	20.380389519469087	23.430656842890976	33.347735860162736	22.841217777477205
23	19.530452577488724	24.98957382296915	31.649302228884956	23.830671370657168
24	20.819103010629064	26.020356406181318	29.795008209440294	23.36553237374933
25	21.326134574705907	25.98472580719901	27.688983068890415	25.000156549204668
26	18.39102484623737	26.022172376955453	29.66068899183564	25.926113784971527
27	18.175487901251266	24.934405883244352	33.8386115463179	23.051494669186482
28	16.817830578693528	25.765306441937152	31.779112829395185	25.63775014997414
29	19.315604449003157	25.261030143862456	31.288675481013083	24.1346899261213
30	22.335563846401435	23.947269218293464	31.557501775267983	22.15966516003712
31	20.384084080699235	26.091492364782187	31.09361517199748	22.430808382521093
32	22.513153264176154	25.04449128396648	28.07822701137549	24.36412844048187
33	22.121028816325204	23.503608772265995	28.513371180669054	25.86199123073975
34	18.345124619428884	23.442304103718232	32.46473572615662	25.74783555069627
35	18.02344731367827	24.76796276884195	31.005321420565057	26.203268496914728
36	20.385399094018442	24.54466098330434	30.73048363685093	24.339456285826287
37	18.539245633216485	27.88166382999508	31.1311243614358	22.447966175352644
38	21.04985653830884	26.06882403994635	27.734883295698904	25.146436126045906
39	20.265357163879465	22.34376702472601	28.869802409855836	28.521073401538693
40	21.1032085072595	21.118550329316907	31.945493324115716	25.832747839307878
41	18.398977545834477	21.94481703155155	29.99351260095858	29.66269282165539
42	18.16008345951199	22.22190912381289	31.066438230067217	28.551569186607907
43	19.624695198698515	22.11902498650546	31.428755709349492	26.82752410544654
44	20.673512250288365	22.507955830581196	29.96840210852993	26.85012981060051
45	19.99158391475708	21.039399051437062	29.363746460422487	29.60527057338338
46	19.909364272465748	21.37829676970109	32.582085009975316	26.130253947857845
47	16.340105025730427	23.212740349993926	35.17629319036008	25.27086143391557
48	17.22899140983204	24.497257884131045	31.95094123643814	26.322809469598766
49	21.30478126318927	20.88153483345043	32.37612887631486	25.437555027045438
50	19.790950454055313	20.82705571022617	30.0459878943631	29.336005941355413
51	17.8156125895618	21.078912070695115	28.782197474923947	32.32327786481913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	741.5
2	1238.0
3	3705.5
4	6173.0
5	4056.0
6	1939.0
7	2025.0
8	2111.0
9	2299.5
10	2488.0
11	2617.5
12	2747.0
13	2723.0
14	2699.0
15	2697.0
16	2695.0
17	2829.0
18	2963.0
19	3112.5
20	3262.0
21	3875.0
22	4488.0
23	5795.5
24	7103.0
25	8662.0
26	12319.5
27	14418.0
28	16318.5
29	18219.0
30	22001.5
31	25784.0
32	29295.5
33	32807.0
34	38258.0
35	43709.0
36	47684.0
37	51659.0
38	58064.5
39	64470.0
40	73005.5
41	81541.0
42	90188.0
43	98835.0
44	108365.0
45	117895.0
46	138897.0
47	159899.0
48	186336.0
49	212773.0
50	202440.5
51	192108.0
52	157365.0
53	122622.0
54	103985.5
55	85349.0
56	75784.5
57	66220.0
58	58748.5
59	51277.0
60	43606.5
61	35936.0
62	30359.0
63	24782.0
64	20910.5
65	17039.0
66	14015.5
67	10992.0
68	8820.5
69	6649.0
70	5656.5
71	4664.0
72	3735.5
73	2807.0
74	2426.0
75	1612.5
76	1180.0
77	844.5
78	509.0
79	348.5
80	188.0
81	142.0
82	96.0
83	77.0
84	58.0
85	45.5
86	33.0
87	18.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1596942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.96790763651107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.07647143074705	19.140971213524427
2	13.810078942784996	8.000981825350443
3	5.82762182886184	5.064420326369564
4	3.0435635667963337	3.526626731552255
5	1.8105452463967104	2.6223853734672042
6	1.1635629957184208	2.022359123354
7	0.8612761394844525	1.7464557360681512
8	0.6500961848524208	1.5065540990122521
9	0.47780458020453487	1.2456899052900219
>10	5.865022252165155	39.492006947948234
>50	0.31119208062801706	5.663193772015584
>100	0.09312371320654743	5.077070801818345
>500	0.0054778625872008635	1.0389242142296287
>1k	0.0032867175523205185	1.7451235548649444
>5k	6.573435104641036E-4	1.471809303491974
>10k+	2.1911450348803457E-4	0.6354270716430115
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	10011	0.626885635170219	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	8923	0.5587554212989576	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	7944	0.4974507527511957	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	6321	0.3958190090811063	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3842	0.24058481773289198	No Hit
TCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	3399	0.21284429866582508	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	3053	0.19117788873985406	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT	2121	0.13281634523983962	No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1849	0.11578379177202427	TruSeq Adapter, Index 20 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1818	0.11384258163414827	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	1785	0.11177613213253831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5810480280435983	0.0
2	0.0	0.0	0.0	2.198389171303654	0.0
3	0.0	0.0	0.0	3.2036229243140952	0.0
4	0.0	0.0	0.0	4.209044536370137	0.0
5	0.0	0.0	0.0	5.792758910467631	0.0
6	0.0	0.0	0.0	6.897495337964685	0.0
7	0.0	0.0	0.0	7.911433226754635	0.0
8	0.0	0.0	0.0	9.537228027066732	0.0
9	0.0	0.0	0.0	10.373326019354492	0.0
10	0.0	0.0	0.0	11.828169088169764	0.0
11	0.0	0.0	0.0	14.441351032160217	0.0
12	0.0	0.0	0.0	16.805118783274533	0.0
13	6.261968186696825E-5	0.0	0.0	17.575153011192644	0.0
14	6.261968186696825E-5	0.0	0.0	17.8707805292866	0.0
15	6.261968186696825E-5	0.0	0.0	18.218194524284538	0.0
16	6.261968186696825E-5	0.0	0.0	19.022544337865746	0.0
17	6.261968186696825E-5	0.0	0.0	20.240747628905748	0.0
18	1.252393637339365E-4	0.0	0.0	21.51775080121883	0.0
19	1.8785904560090472E-4	0.0	0.0	22.470822359234088	0.0
20	1.8785904560090472E-4	0.0	0.0	23.495468213623287	0.0
21	1.8785904560090472E-4	0.0	0.0	24.898775284262047	0.0
22	2.50478727467873E-4	0.0	0.0	26.466709498529063	0.0
23	2.50478727467873E-4	0.0	0.0	27.835074786686054	0.0
24	2.50478727467873E-4	0.0	0.0	28.841122595560766	0.0
25	2.50478727467873E-4	0.0	0.0	29.732952104710126	0.0
26	2.50478727467873E-4	0.0	0.0	30.532292343741975	0.0
27	2.50478727467873E-4	0.0	0.0	31.476033569159057	0.0
28	2.50478727467873E-4	0.0	0.0	32.32766124254982	0.0
29	2.50478727467873E-4	0.0	0.0	33.29770273435103	0.0
30	2.50478727467873E-4	0.0	0.0	34.33944376188991	0.0
31	2.50478727467873E-4	0.0	0.0	35.20804136906663	0.0
32	2.50478727467873E-4	0.0	0.0	36.03643714048475	0.0
33	2.50478727467873E-4	0.0	0.0	36.84166362961209	0.0
34	2.50478727467873E-4	0.0	0.0	37.67951497299213	0.0
35	2.50478727467873E-4	0.0	0.0	38.57698025350952	0.0
36	2.50478727467873E-4	0.0	0.0	39.35421574484233	0.0
37	2.50478727467873E-4	0.0	0.0	40.199644069728265	0.0
38	2.50478727467873E-4	0.0	0.0	41.17601014939804	0.0
39	2.50478727467873E-4	0.0	0.0	42.92203473889471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATG	25	3.891491E-5	45.0	2
TAACCGC	20	7.0341135E-4	45.0	17
CCGCCGT	25	3.891491E-5	45.0	36
ACGTTAT	60	3.6379788E-12	41.249996	24
CGTTTTT	2040	0.0	41.02941	1
TACGGGA	295	0.0	40.42373	4
TTTGGGA	11530	0.0	39.301823	4
TTGGGAC	5100	0.0	38.823532	5
TTGGGAT	7035	0.0	38.69936	5
CTAAACG	35	6.2498184E-6	38.571426	45
GCGCGAC	260	0.0	38.07692	9
TTATCCG	65	9.094947E-12	38.07692	1
GCGATTC	480	0.0	37.968746	9
TTTGGGC	3430	0.0	37.78426	4
TTTTCCG	1005	0.0	37.61194	1
TGGGCGA	2100	0.0	37.607143	6
CAACCCG	90	0.0	37.5	23
CGCGGTA	60	1.5643309E-10	37.499996	31
GCGATCG	30	1.1401211E-4	37.499996	9
ACCGTAC	30	1.1401211E-4	37.499996	22
>>END_MODULE