##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457575
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34412500682948	33.0	31.0	34.0	31.0	34.0
2	32.55522482653117	34.0	31.0	34.0	31.0	34.0
3	32.627999781456595	34.0	31.0	34.0	31.0	34.0
4	36.03416052013331	37.0	35.0	37.0	35.0	37.0
5	36.06970004917227	37.0	35.0	37.0	35.0	37.0
6	35.48915259793476	37.0	35.0	37.0	33.0	37.0
7	35.932011145713815	37.0	35.0	37.0	35.0	37.0
8	35.85931049554718	37.0	35.0	37.0	35.0	37.0
9	37.75112495219363	39.0	38.0	39.0	35.0	39.0
10	37.531128230344756	39.0	37.0	39.0	35.0	39.0
11	37.516249795115556	39.0	37.0	39.0	35.0	39.0
12	37.29379883079277	39.0	37.0	39.0	35.0	39.0
13	37.129956837676886	39.0	37.0	39.0	33.0	39.0
14	38.3518745560837	40.0	38.0	41.0	34.0	41.0
15	38.384898650494456	40.0	38.0	41.0	34.0	41.0
16	38.532188165874445	40.0	38.0	41.0	35.0	41.0
17	38.451134786646996	40.0	38.0	41.0	34.0	41.0
18	38.43258263672622	40.0	37.0	41.0	34.0	41.0
19	38.45105174015189	40.0	37.0	41.0	34.0	41.0
20	38.315878271321644	40.0	37.0	41.0	34.0	41.0
21	38.3034606348686	40.0	37.0	41.0	34.0	41.0
22	38.27327541933016	40.0	37.0	41.0	34.0	41.0
23	38.19576462874939	40.0	37.0	41.0	34.0	41.0
24	38.1744085669016	40.0	37.0	41.0	34.0	41.0
25	38.10262798448342	40.0	37.0	41.0	34.0	41.0
26	37.99134131016773	40.0	36.0	41.0	34.0	41.0
27	37.97002458613342	40.0	36.0	41.0	34.0	41.0
28	37.90679779271158	40.0	36.0	41.0	34.0	41.0
29	37.884019013276514	40.0	36.0	41.0	34.0	41.0
30	37.83486204447358	40.0	36.0	41.0	33.0	41.0
31	37.748961372452605	40.0	36.0	41.0	33.0	41.0
32	37.610686772660216	40.0	36.0	41.0	33.0	41.0
33	37.545552095284926	40.0	36.0	41.0	33.0	41.0
34	37.41432333497241	40.0	36.0	41.0	33.0	41.0
35	37.42299295197508	40.0	36.0	41.0	33.0	41.0
36	37.34017592744359	40.0	35.0	41.0	33.0	41.0
37	37.24406709282631	40.0	35.0	41.0	33.0	41.0
38	37.2586570507567	40.0	35.0	41.0	32.0	41.0
39	37.158111784953284	39.0	35.0	41.0	32.0	41.0
40	36.95120144238649	39.0	35.0	41.0	31.0	41.0
41	36.76138556520789	39.0	35.0	41.0	31.0	41.0
42	36.87257826585805	39.0	35.0	41.0	31.0	41.0
43	36.86633885155439	39.0	35.0	41.0	32.0	41.0
44	36.80738021089439	39.0	35.0	41.0	31.0	41.0
45	36.81614817243075	39.0	35.0	41.0	32.0	41.0
46	36.69109981970169	39.0	35.0	41.0	31.0	41.0
47	36.532876577610224	39.0	35.0	40.0	31.0	41.0
48	36.52070589520844	39.0	35.0	40.0	31.0	41.0
49	36.57841446757362	39.0	35.0	40.0	31.0	41.0
50	36.463331694257775	39.0	35.0	40.0	31.0	41.0
51	35.5642004043053	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	13.0
11	6.0
12	5.0
13	2.0
14	9.0
15	8.0
16	21.0
17	20.0
18	64.0
19	118.0
20	265.0
21	535.0
22	875.0
23	1172.0
24	1499.0
25	1995.0
26	2515.0
27	3123.0
28	3678.0
29	4366.0
30	5600.0
31	7414.0
32	10057.0
33	14021.0
34	26560.0
35	39008.0
36	33590.0
37	52499.0
38	98304.0
39	150136.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.58651587171502	4.410424520570398	51.33737638638475	16.665683221329836
2	18.227175872807734	11.513522373381413	50.52242801726493	19.73687373654592
3	19.793476479265696	11.11511774026116	47.883297820029505	21.20810796044364
4	18.01781128776703	4.687537562148282	53.89695678304103	23.397694367043652
5	22.590395017210295	5.423154674097143	46.73310386275474	25.253346445937826
6	20.460252417636454	11.827787794350652	49.79118177347976	17.920778014533138
7	77.8228705676665	2.5040703709774355	14.650275911052832	5.022783150303229
8	77.30404851663661	8.23121892585915	8.752444954379063	5.71228760312517
9	71.10134950554554	6.174069824618915	10.854176910888926	11.87040375894662
10	38.84521663115336	27.59788012894061	18.473037207015246	15.083866032890784
11	32.359503906463424	24.135933999890728	24.189477134895917	19.31508495874993
12	28.893405452658033	19.728132000218544	29.388843359012185	21.989619188111238
13	24.23231164290007	24.389225809976505	31.80156258536852	19.576899961754904
14	19.450800415232475	29.362836693438233	27.619515926350875	23.56684696497842
15	16.591378462547123	24.050264983882425	38.758454898104134	20.59990165546632
16	17.9859039501721	22.9107796536087	33.325247227230506	25.778069168988686
17	18.35546085341201	23.27072064688849	31.23291263727258	27.140905862426923
18	19.426760640332187	23.606403321859805	31.822979839370596	25.143856198437415
19	22.137791618860298	26.035731847238157	28.56318636289133	23.26329017101022
20	25.719936622411627	24.477298803474838	30.756488007430477	19.04627656668306
21	24.010052996776484	27.93662241162651	27.293886248156042	20.759438343440966
22	20.4370868163689	24.980604272523628	28.615855324263784	25.966453586843684
23	21.51428727531006	27.72856908703491	27.323608151669127	23.433535485985903
24	22.676064033218598	22.973938698574003	28.750259520297217	25.59973774791018
25	19.82669507730973	27.984920504835276	26.924766431732504	25.263617986122494
26	18.81680598808938	28.360596623504343	28.41654373599956	24.406053652406708
27	20.902802819209963	27.925039610992737	28.529093591214554	22.643063978582745
28	17.352128066437196	26.501229306671036	32.40124569742665	23.745396929465116
29	21.561492651477902	23.96088072993498	29.712506146533357	24.765120472053763
30	21.0920614106977	26.30650712997869	29.570452931213463	23.030978528110147
31	22.421023875867345	28.000874173632738	23.971807900344206	25.606294050155714
32	25.57853903731629	29.959460197781784	23.071627602032454	21.390373162869476
33	22.88236901054472	26.21297055127575	24.64120635961318	26.263454078566355
34	21.464240834835817	28.498060427252366	25.282412719226354	24.75528601868546
35	20.618914931978363	26.717150193957274	26.34803037753374	26.315904496530624
36	21.449816969895647	27.98819865595804	25.134021745069113	25.4279626290772
37	21.5909960115828	26.72567338687647	29.828989783095665	21.854340818445063
38	21.724088947167132	25.51253892804458	24.338086652461346	28.425285472326937
39	25.767579085395838	25.818718242910997	25.736983008250014	22.67671966344315
40	24.194722176692345	25.629022564606895	27.909304485603453	22.266950773097307
41	17.375293667704746	28.94629295743867	25.999016554663168	27.679396820193407
42	19.992569524121727	26.801507949516473	28.05070207069879	25.155220455663006
43	22.72720319073376	23.23444244112987	26.3864940173742	27.65186035076217
44	21.896082609408293	24.035404032125882	28.280391192700648	25.788122165765177
45	21.601486095175655	23.100256788504616	25.675790853958368	29.622466262361364
46	24.15363601595367	26.3373217505327	26.11834125553188	23.39070097798175
47	18.90706441566956	24.517729333988964	32.93711413429492	23.63809211604655
48	21.175763536032342	24.188821504671367	27.729224717259466	26.906190242036825
49	20.629842102387585	22.21712287603125	32.102715401846694	25.050319619734466
50	22.021745069114353	22.661640168278424	27.830628858657054	27.485985903950173
51	18.999071190515217	23.738622083811396	25.650439818608973	31.611866907064417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	200.5
2	351.0
3	829.5
4	1308.0
5	958.0
6	608.0
7	632.5
8	657.0
9	673.0
10	689.0
11	726.5
12	764.0
13	758.0
14	752.0
15	701.0
16	650.0
17	660.5
18	671.0
19	734.0
20	797.0
21	958.5
22	1120.0
23	1272.0
24	1424.0
25	1909.0
26	2802.0
27	3210.0
28	3886.0
29	4562.0
30	4973.0
31	5384.0
32	6728.5
33	8073.0
34	9902.5
35	11732.0
36	12295.5
37	12859.0
38	14439.5
39	16020.0
40	18680.0
41	21340.0
42	24300.5
43	27261.0
44	30216.5
45	33172.0
46	47721.0
47	62270.0
48	58301.0
49	54332.0
50	51227.0
51	48122.0
52	40213.5
53	32305.0
54	27354.0
55	22403.0
56	20504.5
57	18606.0
58	18076.5
59	17547.0
60	16896.5
61	16246.0
62	13723.0
63	11200.0
64	9092.5
65	6985.0
66	5682.5
67	4380.0
68	3487.0
69	2594.0
70	2319.0
71	2044.0
72	1585.5
73	1127.0
74	954.5
75	564.0
76	346.0
77	272.0
78	198.0
79	163.5
80	129.0
81	104.0
82	79.0
83	52.5
84	26.0
85	15.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.8315429937557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08935555630626	21.20705166676336
2	12.147294713882191	7.247450890299959
3	4.673887872832882	4.1828786107922244
4	2.397763492892558	2.861159389083288
5	1.3523260412252123	2.0170986220192684
6	0.8700487445773776	1.5572937918313927
7	0.624199125248561	1.3034576129062005
8	0.5085615756621731	1.213694120747061
9	0.39312362278120627	1.05547358293727
>10	5.500382455485965	39.88314371391777
>50	0.3732551475052088	6.914959531082529
>100	0.05878033818979666	3.115984046337937
>500	0.0022042626821173745	0.4293903170213857
>1k	0.007347542273724582	4.369523210730639
>5k	0.0014695084547449164	2.6414408935297184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	6653	1.45396929465115	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	5398	1.1796973173796645	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	4935	1.0785117193902638	RNA PCR Primer, Index 30 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2798	0.6114844561000929	RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2398	0.5240670928263126	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2149	0.4696497841883844	RNA PCR Primer, Index 30 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1603	0.3503250833196744	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	1368	0.2989673823963285	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	1280	0.2797355624760968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	1223	0.2672785882095831	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	1145	0.250232202371196	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	1036	0.22641097087909087	No Hit
TCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	701	0.1531989291372999	RNA PCR Primer, Index 30 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.15035786483090205	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	570	0.12456974266513686	RNA PCR Primer, Index 30 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4915041250068295	0.0
2	0.0	0.0	0.0	2.0943014806315903	0.0
3	0.0	0.0	0.0	2.8366934382341693	0.0
4	0.0	0.0	0.0	3.835436813637109	0.0
5	0.0	0.0	0.0	6.1950499918046225	0.0
6	0.0	0.0	0.0	7.04540239305032	0.0
7	0.0	0.0	0.0	8.082390864885538	0.0
8	0.0	0.0	0.0	9.493088564716167	0.0
9	0.0	0.0	0.0	10.1222750368792	0.0
10	0.0	0.0	0.0	12.495874993170519	0.0
11	0.0	0.0	0.0	14.359176091351145	0.0
12	0.0	0.0	0.0	17.7048571272469	0.0
13	2.1854340818445065E-4	0.0	0.0	18.40113642572256	0.0
14	2.1854340818445065E-4	0.0	0.0	18.703381959241653	0.0
15	2.1854340818445065E-4	0.0	0.0	19.353330055182212	0.0
16	2.1854340818445065E-4	0.0	0.0	20.23580833743102	0.0
17	2.1854340818445065E-4	0.0	0.0	21.36371086707097	0.0
18	2.1854340818445065E-4	0.0	0.0	22.566792329126372	0.0
19	2.1854340818445065E-4	0.0	0.0	23.661039173905916	0.0
20	2.1854340818445065E-4	0.0	0.0	24.536524067092827	0.0
21	2.1854340818445065E-4	0.0	0.0	25.58553242637819	0.0
22	2.1854340818445065E-4	0.0	0.0	26.734415123203846	0.0
23	2.1854340818445065E-4	0.0	0.0	27.746708189914223	0.0
24	2.1854340818445065E-4	0.0	0.0	28.549636671583894	0.0
25	2.1854340818445065E-4	0.0	0.0	29.26143255204065	0.0
26	2.1854340818445065E-4	0.0	0.0	29.915314429328525	0.0
27	2.1854340818445065E-4	0.0	0.0	30.54646779216522	0.0
28	2.1854340818445065E-4	0.0	0.0	31.14068731901874	0.0
29	2.1854340818445065E-4	0.0	0.0	31.74124460470961	0.0
30	2.1854340818445065E-4	0.0	0.0	32.43490138228706	0.0
31	2.1854340818445065E-4	0.0	0.0	33.11304157788341	0.0
32	2.1854340818445065E-4	0.0	0.0	33.75359230727203	0.0
33	2.1854340818445065E-4	0.0	0.0	34.40681855433535	0.0
34	2.1854340818445065E-4	0.0	0.0	35.032508331967435	0.0
35	2.1854340818445065E-4	0.0	0.0	35.67874118996886	0.0
36	2.1854340818445065E-4	0.0	0.0	36.341146260175925	0.0
37	2.1854340818445065E-4	0.0	0.0	37.00158443970934	0.0
38	2.1854340818445065E-4	0.0	0.0	37.59624105337923	0.0
39	2.1854340818445065E-4	0.0	0.0	38.21144074741846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	25	3.887112E-5	45.000004	19
CGGGTAC	50	2.1827873E-11	45.000004	6
AATCCCG	20	7.0288294E-4	45.000004	13
GATCGCC	25	3.887112E-5	45.000004	35
CGAAACG	20	7.0288294E-4	45.000004	33
CATGCGT	20	7.0288294E-4	45.000004	11
ACGTTAT	20	7.0288294E-4	45.000004	30
CGTGACA	20	7.0288294E-4	45.000004	44
GGCCGTC	20	7.0288294E-4	45.000004	8
GGCGTAG	20	7.0288294E-4	45.000004	1
GACAACG	20	7.0288294E-4	45.000004	33
GATTTCG	20	7.0288294E-4	45.000004	10
TATTGCG	25	3.887112E-5	45.000004	1
AACGGTT	20	7.0288294E-4	45.000004	36
TAGCAAC	20	7.0288294E-4	45.000004	21
AACGGCG	20	7.0288294E-4	45.000004	14
AACGGCC	20	7.0288294E-4	45.000004	37
ATATCAC	110	0.0	45.000004	30
TACGCGG	25	3.887112E-5	45.000004	2
AGGCCGT	20	7.0288294E-4	45.000004	7
>>END_MODULE