##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370312
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.389250145822984	33.0	31.0	34.0	31.0	34.0
2	32.609024282226876	34.0	31.0	34.0	31.0	34.0
3	32.66244950204152	34.0	31.0	34.0	31.0	34.0
4	36.0490046231286	37.0	35.0	37.0	35.0	37.0
5	36.09172265549051	37.0	35.0	37.0	35.0	37.0
6	35.73725399122902	37.0	35.0	37.0	35.0	37.0
7	36.06309544384195	37.0	35.0	37.0	35.0	37.0
8	36.06896346864266	37.0	36.0	37.0	35.0	37.0
9	37.863207241461254	39.0	38.0	39.0	35.0	39.0
10	37.57166389422973	39.0	37.0	39.0	35.0	39.0
11	37.513980103264274	39.0	37.0	39.0	35.0	39.0
12	37.24859577869472	39.0	37.0	39.0	35.0	39.0
13	37.064037892371836	39.0	37.0	39.0	33.0	39.0
14	38.24447492924885	40.0	37.0	41.0	33.0	41.0
15	38.35545431960077	40.0	37.0	41.0	34.0	41.0
16	38.42894100110178	40.0	37.0	41.0	34.0	41.0
17	38.42159314307935	40.0	37.0	41.0	34.0	41.0
18	38.398847458359434	40.0	37.0	41.0	34.0	41.0
19	38.41190401607293	40.0	37.0	41.0	34.0	41.0
20	38.36917788243427	40.0	37.0	41.0	34.0	41.0
21	38.27342889239344	40.0	37.0	41.0	34.0	41.0
22	38.24817451230314	40.0	37.0	41.0	34.0	41.0
23	38.221194560262695	40.0	36.0	41.0	34.0	41.0
24	38.1769291840394	40.0	36.0	41.0	34.0	41.0
25	38.0871292315669	40.0	36.0	41.0	34.0	41.0
26	38.01078549979477	40.0	36.0	41.0	34.0	41.0
27	37.981766726436085	40.0	36.0	41.0	34.0	41.0
28	37.89110263777572	40.0	36.0	41.0	34.0	41.0
29	37.79636630733003	40.0	36.0	41.0	34.0	41.0
30	37.75989435935104	40.0	36.0	41.0	34.0	41.0
31	37.682654086283996	40.0	36.0	41.0	33.0	41.0
32	37.5515538248828	40.0	35.0	41.0	33.0	41.0
33	37.49232809090713	40.0	35.0	41.0	33.0	41.0
34	37.513602043682084	40.0	35.0	41.0	33.0	41.0
35	37.400116658385365	40.0	35.0	41.0	33.0	41.0
36	37.316497980081664	39.0	35.0	41.0	33.0	41.0
37	37.201981032210675	39.0	35.0	41.0	32.0	41.0
38	37.103952882974355	39.0	35.0	41.0	32.0	41.0
39	37.060783879539414	39.0	35.0	41.0	32.0	41.0
40	37.034781481561495	39.0	35.0	41.0	32.0	41.0
41	37.01886787357687	39.0	35.0	41.0	32.0	41.0
42	37.023998682192314	39.0	35.0	41.0	32.0	41.0
43	36.854898031929835	39.0	35.0	41.0	32.0	41.0
44	36.661061483289764	39.0	35.0	41.0	31.0	41.0
45	36.6231826135799	39.0	35.0	41.0	31.0	41.0
46	36.54337693620515	39.0	35.0	40.0	31.0	41.0
47	36.415933051048846	38.0	35.0	40.0	31.0	41.0
48	36.30407332195554	38.0	35.0	40.0	31.0	41.0
49	36.239030871265314	38.0	35.0	40.0	31.0	41.0
50	36.07951403141135	38.0	35.0	40.0	31.0	41.0
51	35.11556201257318	36.0	34.0	39.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	12.0
11	7.0
12	7.0
13	3.0
14	6.0
15	4.0
16	11.0
17	12.0
18	40.0
19	69.0
20	152.0
21	300.0
22	421.0
23	598.0
24	953.0
25	1362.0
26	1994.0
27	2556.0
28	2900.0
29	3533.0
30	4479.0
31	5898.0
32	7718.0
33	11502.0
34	23212.0
35	37301.0
36	27444.0
37	43744.0
38	82422.0
39	111558.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.989500745317464	4.68496835101212	54.53050400743157	16.795026896238845
2	19.434152822484823	4.939618483873058	52.65613860744453	22.970090086197583
3	20.852416340815314	4.282334895979607	52.496273412689845	22.368975350515242
4	19.642085592689405	4.407364600661064	50.738026312946914	25.21252349370261
5	18.161982328414958	6.113493486573484	50.69697984402342	25.02754434098814
6	21.484315928190284	6.465358940569034	52.700695629631234	19.349629501609453
7	82.41077793860312	2.3831255805915013	9.584620536196505	5.6214759446088705
8	82.75346194560262	3.039058955691417	8.01351292963771	6.193966169068245
9	75.22953617490117	6.479671196180519	11.802750113417874	6.488042515500443
10	38.19238912052539	27.261876471731945	19.397427034500637	15.148307373242023
11	31.578776815226078	25.27490332476398	24.34352653978267	18.802793320227266
12	29.71008230897189	21.443809544384195	28.45033377260256	20.39577437404135
13	23.382445073343558	26.811715526366957	30.443247855862083	19.362591544427403
14	18.51276761217568	30.159973211778173	29.130030892868714	22.197228283177427
15	15.61035019119013	25.653773034630255	38.27691244140076	20.458964332778844
16	17.150672946056297	24.756691654604765	34.95349867139061	23.139136727948326
17	17.578420359048586	24.5754930977122	29.796765970316923	28.049320572922294
18	19.74092116917626	24.12722245025816	32.86498952234872	23.266866858216854
19	22.848030849661907	26.352913219123337	29.882909546544532	20.916146384670224
20	25.84496316619499	26.02697185076368	28.505692497137545	19.622372485903778
21	22.855321998747	27.627514096221567	29.003650975393725	20.513512929637713
22	20.81028970165698	25.82362980405712	27.289690855278792	26.076389639007107
23	20.123571474864438	27.36854328242131	26.754196461362312	25.75368878135194
24	22.16455313357385	23.25066430469442	30.35116334334291	24.233619218388817
25	19.161949923307915	25.75395882391065	29.11490850958111	25.96918274320033
26	20.559960249735358	28.547009008619757	27.476020220786797	23.417010520858085
27	22.607963014971162	28.495430879906674	27.85137937738988	21.04522672773229
28	21.038475663764608	25.86872691136123	31.70839724340556	21.3844001814686
29	20.08036466547128	23.661939121605567	32.29249929788935	23.96519691503381
30	23.173702175462854	26.117976193048026	27.594298861500572	23.11402276998855
31	23.752673421331203	28.064983041327313	26.03615329775973	22.14619023958176
32	24.03000712912355	28.087666616258723	26.533571690898484	21.34875456371924
33	24.24739138888289	27.663699799088338	25.891140443733935	22.19776836829484
34	21.53508392922725	25.687258311909954	28.104139212339863	24.67351854652293
35	21.406273628723888	24.041348916589257	28.60155760547862	25.950819849208234
36	25.75746937717384	25.037535915660307	26.595411436842447	22.6095832703234
37	23.541230097863423	26.999935189785912	29.375769621292314	20.08306509105835
38	20.26858432889023	27.492222774309234	29.39035191946251	22.84884097733803
39	20.296668754995785	26.968340210417164	29.537795156516665	23.197195878070385
40	22.289042753137895	25.78123312234008	28.788427056104045	23.14129706841798
41	19.039620644213528	26.156592278943165	28.35149819611571	26.452288880727604
42	22.879625829030655	25.523612521333362	27.503564561774933	24.093197087861046
43	24.684050206312516	23.81073257145326	27.82464516407786	23.680572058156365
44	22.10190325995377	24.603847566376462	27.59483894661799	25.699410227051782
45	21.602324526345352	25.019983149344338	26.456069476549505	26.92162284776081
46	23.03219987470025	24.73967897340621	29.023904167296767	23.204216984596773
47	20.380651990753744	25.512540776426363	30.626876795783016	23.479930437036877
48	20.82703234029683	22.9482166389423	31.391367279483244	24.833383741277625
49	21.86696623387846	22.023320875369958	31.955756227181404	24.153956663570177
50	21.03874570632332	23.10322106764026	29.529423837196745	26.32860938883968
51	19.327756054354168	23.59847912030936	27.175192810386918	29.898572014949554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	151.5
2	276.0
3	642.5
4	1009.0
5	723.5
6	438.0
7	464.0
8	490.0
9	566.0
10	642.0
11	687.0
12	732.0
13	729.5
14	727.0
15	699.0
16	671.0
17	599.0
18	527.0
19	527.5
20	528.0
21	631.0
22	734.0
23	1003.0
24	1272.0
25	1516.5
26	2357.5
27	2954.0
28	3287.0
29	3620.0
30	4464.0
31	5308.0
32	6434.5
33	7561.0
34	8147.5
35	8734.0
36	9890.5
37	11047.0
38	11586.5
39	12126.0
40	15550.0
41	18974.0
42	22055.0
43	25136.0
44	27838.0
45	30540.0
46	34587.5
47	38635.0
48	43454.0
49	48273.0
50	45925.5
51	43578.0
52	36334.0
53	29090.0
54	24401.5
55	19713.0
56	17112.5
57	14512.0
58	13176.0
59	11840.0
60	10785.0
61	9730.0
62	8377.0
63	7024.0
64	5699.0
65	4374.0
66	3576.0
67	2778.0
68	2389.5
69	2001.0
70	1545.0
71	1089.0
72	928.5
73	768.0
74	647.5
75	366.0
76	205.0
77	184.0
78	163.0
79	128.0
80	93.0
81	85.0
82	77.0
83	41.5
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.157256118127094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.69740053093794	18.498585330447987
2	14.477884487785882	8.153150031424927
3	5.944996717503252	5.021843855884918
4	3.0639686846236023	3.4509180396347103
5	1.7545256571943606	2.4701314147723434
6	1.1380261435798384	1.9226216156341194
7	0.7626526191567655	1.503194358873024
8	0.6033499455079121	1.3590943155619446
9	0.43110625583397527	1.0924892333679659
>10	5.303701590274937	37.03361502100929
>50	0.7317853694759402	12.59376153729147
>100	0.08263698730136466	3.6769533718388114
>500	0.00398250541211396	0.7431841558969723
>1k	0.00398250541211396	2.480457718361528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2800	0.7561191643803064	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	2323	0.627308863876947	Illumina PCR Primer Index 7 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1964	0.5303635853010434	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1761	0.47554494588347124	Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	860	0.23223660048823694	Illumina PCR Primer Index 7 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	627	0.16931668430944716	Illumina PCR Primer Index 7 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	584	0.1577048542850353	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	580	0.15662468405020633	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	420	0.11341787465704595	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6564734602173302	0.0
2	0.0	0.0	0.0	2.572425414245285	0.0
3	0.0	0.0	0.0	3.669338287714144	0.0
4	0.0	0.0	0.0	4.639331158590594	0.0
5	0.0	0.0	0.0	6.255265829894792	0.0
6	0.0	0.0	0.0	7.265495042018622	0.0
7	0.0	0.0	0.0	8.198762124910886	0.0
8	0.0	0.0	0.0	9.870325563308777	0.0
9	0.0	0.0	0.0	10.75444490051632	0.0
10	0.0	0.0	0.0	12.223746462442481	0.0
11	0.0	0.0	0.0	14.653589405690337	0.0
12	0.0	0.0	0.0	16.764242044546222	0.0
13	0.0	0.0	0.0	17.484985633735878	0.0
14	0.0	0.0	0.0	17.72235304283955	0.0
15	0.0	0.0	0.0	18.104193220851606	0.0
16	2.7004255870725224E-4	0.0	0.0	19.00721553716866	0.0
17	2.7004255870725224E-4	0.0	0.0	20.2934282442913	0.0
18	5.400851174145045E-4	0.0	0.0	21.91206334118257	0.0
19	5.400851174145045E-4	0.0	0.0	22.79267212512692	0.0
20	5.400851174145045E-4	0.0	0.0	23.791829592343753	0.0
21	5.400851174145045E-4	0.0	0.0	25.05400851174145	0.0
22	5.400851174145045E-4	0.0	0.0	26.261368791721576	0.0
23	5.400851174145045E-4	0.0	0.0	27.444695283976756	0.0
24	5.400851174145045E-4	0.0	0.0	28.345557259824147	0.0
25	5.400851174145045E-4	0.0	0.0	29.16594655317678	0.0
26	5.400851174145045E-4	0.0	0.0	29.83808248179913	0.0
27	5.400851174145045E-4	0.0	0.0	30.498336537838362	0.0
28	5.400851174145045E-4	0.0	0.0	31.16669187063881	0.0
29	5.400851174145045E-4	0.0	0.0	31.946844822744065	0.0
30	5.400851174145045E-4	0.0	0.0	32.7747953077405	0.0
31	5.400851174145045E-4	0.0	0.0	33.57655166454233	0.0
32	5.400851174145045E-4	0.0	0.0	34.32078895633952	0.0
33	5.400851174145045E-4	0.0	0.0	35.00075611916438	0.0
34	5.400851174145045E-4	0.0	0.0	35.70232668668582	0.0
35	5.400851174145045E-4	0.0	0.0	36.429551297284455	0.0
36	5.400851174145045E-4	0.0	0.0	37.11032858778543	0.0
37	5.400851174145045E-4	0.0	0.0	37.8089286871611	0.0
38	5.400851174145045E-4	0.0	0.0	38.472693296463525	0.0
39	5.400851174145045E-4	0.0	0.0	39.132947352502754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCTA	30	2.1615015E-6	45.000004	29
ACCTAAG	30	2.1615015E-6	45.000004	31
GTTAAGG	30	2.1615015E-6	45.000004	28
GAGCGTA	30	2.1615015E-6	45.000004	9
CGGGCCC	30	2.1615015E-6	45.000004	6
CGGGCAA	30	2.1615015E-6	45.000004	6
GTCCCGT	30	2.1615015E-6	45.000004	18
GGCGCTA	30	2.1615015E-6	45.000004	7
TTGTTCG	30	2.1615015E-6	45.000004	1
TTCATCG	60	0.0	45.000004	15
CTTGTCG	20	7.0270867E-4	45.0	1
CTCACGG	25	3.8856677E-5	45.0	2
TCGTTAG	40	6.7939254E-9	45.0	1
CCGGGTA	20	7.0270867E-4	45.0	5
CCGGGAT	40	6.7939254E-9	45.0	5
GGTAATT	20	7.0270867E-4	45.0	8
CGTGCTC	20	7.0270867E-4	45.0	29
GCGGTCA	25	3.8856677E-5	45.0	14
CTATCGG	20	7.0270867E-4	45.0	2
CGGCATA	20	7.0270867E-4	45.0	27
>>END_MODULE