SRR2933924.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933924.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370445
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1993	0.5380015926790752	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	1747	0.47159497361281705	TruSeq Adapter, Index 27 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1713	0.46241682301016346	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1585	0.42786378544723236	TruSeq Adapter, Index 20 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	663	0.17897393675174453	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.16412692842392257	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	530	0.14307117115901147	TruSeq Adapter, Index 27 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	443	0.11958590344045673	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	405	0.1093279704139616	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	30	2.1615033E-6	45.000004	1
AGACATG	30	2.1615033E-6	45.000004	15
TCGACGT	30	2.1615033E-6	45.000004	26
CTAGATC	20	7.027089E-4	45.0	19
AGGTATA	20	7.027089E-4	45.0	25
AACGTAC	20	7.027089E-4	45.0	39
GCCCATA	20	7.027089E-4	45.0	9
ATCTCGA	20	7.027089E-4	45.0	28
GGTACCC	35	1.2089549E-7	45.0	8
TCCGCAT	20	7.027089E-4	45.0	44
GTATTCG	25	3.885669E-5	45.0	12
CTCAACC	45	3.8380676E-10	45.0	36
TACCACG	25	3.885669E-5	45.0	26
ACCATGC	25	3.885669E-5	45.0	15
GCGGTAC	20	7.027089E-4	45.0	32
GGCACGT	20	7.027089E-4	45.0	11
CCGGCTA	25	3.885669E-5	45.0	34
CGTTGGA	40	6.7939254E-9	45.0	3
CGTTGAT	35	1.2089549E-7	45.0	25
TCGGGTA	45	3.8380676E-10	45.0	5