##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933923.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803057
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43190333936445	33.0	31.0	34.0	31.0	34.0
2	32.65704551482647	34.0	31.0	34.0	31.0	34.0
3	32.71186105095902	34.0	31.0	34.0	31.0	34.0
4	36.08640980652681	37.0	35.0	37.0	35.0	37.0
5	36.12318054633731	37.0	35.0	37.0	35.0	37.0
6	35.73513710732862	37.0	35.0	37.0	35.0	37.0
7	36.071774481761565	37.0	35.0	37.0	35.0	37.0
8	36.07238465015559	37.0	36.0	37.0	35.0	37.0
9	37.973837473554184	39.0	39.0	39.0	35.0	39.0
10	37.56719012473585	39.0	37.0	39.0	35.0	39.0
11	37.51781878496794	39.0	37.0	39.0	35.0	39.0
12	37.37913622569755	39.0	37.0	39.0	35.0	39.0
13	37.36157707360748	39.0	37.0	39.0	35.0	39.0
14	38.64603135269352	40.0	38.0	41.0	35.0	41.0
15	38.69012535847393	40.0	38.0	41.0	35.0	41.0
16	38.75519172362609	40.0	38.0	41.0	35.0	41.0
17	38.705580052225436	40.0	38.0	41.0	35.0	41.0
18	38.66852290684223	40.0	38.0	41.0	35.0	41.0
19	38.67494088215407	40.0	38.0	41.0	35.0	41.0
20	38.61497253619606	40.0	38.0	41.0	35.0	41.0
21	38.54933958610659	40.0	38.0	41.0	34.0	41.0
22	38.56096889760005	40.0	38.0	41.0	35.0	41.0
23	38.498812662114894	40.0	38.0	41.0	34.0	41.0
24	38.46015164552454	40.0	38.0	41.0	34.0	41.0
25	38.375441593809654	40.0	37.0	41.0	34.0	41.0
26	38.29674232339672	40.0	37.0	41.0	34.0	41.0
27	38.28943524556787	40.0	37.0	41.0	34.0	41.0
28	38.226439717230534	40.0	37.0	41.0	34.0	41.0
29	38.21985612478317	40.0	37.0	41.0	34.0	41.0
30	38.18896915162934	40.0	37.0	41.0	34.0	41.0
31	38.14594107267604	40.0	37.0	41.0	34.0	41.0
32	38.05339222496037	40.0	37.0	41.0	34.0	41.0
33	37.9796577328882	40.0	37.0	41.0	34.0	41.0
34	37.93895701052354	40.0	37.0	41.0	34.0	41.0
35	37.88759452940451	40.0	37.0	41.0	33.0	41.0
36	37.839248023490235	40.0	37.0	41.0	33.0	41.0
37	37.812546307422764	40.0	37.0	41.0	33.0	41.0
38	37.789712311828424	40.0	37.0	41.0	33.0	41.0
39	37.74742390639768	40.0	37.0	41.0	33.0	41.0
40	37.67233583668407	40.0	36.0	41.0	33.0	41.0
41	37.54643568264768	40.0	36.0	41.0	33.0	41.0
42	37.51139831917286	40.0	36.0	41.0	33.0	41.0
43	37.40271612102254	40.0	36.0	41.0	33.0	41.0
44	37.31865857591678	40.0	36.0	41.0	33.0	41.0
45	37.328969176534166	40.0	35.0	41.0	33.0	41.0
46	37.24901719305105	39.0	35.0	41.0	32.0	41.0
47	37.15536257077642	39.0	35.0	41.0	32.0	41.0
48	37.12074236324445	39.0	35.0	41.0	32.0	41.0
49	37.10798859856772	39.0	35.0	41.0	32.0	41.0
50	37.0252422928883	39.0	35.0	41.0	32.0	41.0
51	36.08995500942025	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	10.0
11	8.0
12	8.0
13	8.0
14	11.0
15	11.0
16	17.0
17	42.0
18	85.0
19	164.0
20	290.0
21	525.0
22	870.0
23	1363.0
24	1905.0
25	2676.0
26	3698.0
27	4382.0
28	5473.0
29	6495.0
30	8423.0
31	11199.0
32	14822.0
33	21115.0
34	39099.0
35	57177.0
36	53849.0
37	86030.0
38	174401.0
39	308660.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.58774034719827	4.650852928247932	57.853178541498295	13.908228183055499
2	18.98545184214819	5.9115355447994355	54.73932734538146	20.36368526767091
3	19.374465324379216	6.160334820566908	52.7155606638134	21.749639191240473
4	18.091990979469703	4.52159684804441	54.1225591707687	23.263853001717187
5	18.802774896427028	5.6807922725286	51.55536904603285	23.961063785011525
6	19.69623575910552	7.351532954696864	55.169309276925546	17.78292200927207
7	84.22714701447094	1.822162063215936	9.604673142753255	4.3460177795598565
8	86.819366495778	3.156687507860588	5.992849822615332	4.031096173746073
9	82.0793542675053	4.219999327569525	7.7791489271620815	5.921497477763098
10	38.09106950067056	26.471221843530408	18.56680160935027	16.870907046448757
11	28.723365838290434	26.918139061112722	24.289807572812393	20.068687527784455
12	24.51158510541593	22.673359425296088	30.182415444980865	22.632640024307115
13	23.572548399428683	23.68686158018671	32.13370906423828	20.606880956146327
14	20.899761785278006	25.983460700797078	30.79233479068111	22.324442723243802
15	18.630682504479758	25.59643960515879	33.98787383709998	21.785004053261474
16	20.1942078831266	25.028484902067973	31.443845206504644	23.33346200830078
17	20.349987609845876	26.50235288404186	29.122963874295348	24.02469563181692
18	21.854613059845068	26.115829885051745	30.53257738865361	21.49697966644958
19	22.890529563903932	27.856055049641558	28.10522789789517	21.14818748855934
20	24.139133336736993	27.026724130416646	29.190455970124162	19.6436865627222
21	23.546398325399068	28.49523757342256	28.10958624356677	19.8487778576116
22	22.293187158570312	25.111044421504324	28.61826744552379	23.97750097440157
23	21.324389177854126	26.90195092004677	27.891419911662556	23.882239990436542
24	21.39599057103045	25.625329210753407	29.331043749073853	23.64763646914229
25	20.447365504565678	28.63682154566861	26.489277847027047	24.42653510273866
26	19.117322929754675	29.149861093297236	28.04757321086797	23.685242766080115
27	20.605137617877688	28.2018586476427	27.637515145251207	23.55548858922841
28	19.06116253267203	27.336540245586555	30.446157620193837	23.156139601547586
29	20.39917465385396	25.100584391892482	29.024838834603273	25.475402119650287
30	21.441939986825343	27.173164544982487	27.891793484148696	23.493101984043474
31	22.69714354024683	27.402413527308774	26.747665483272048	23.15277744917235
32	22.686185413986802	28.612788382393777	25.733540707571194	22.967485496048226
33	21.785253101585567	26.927851945752295	25.903142616277545	25.38375233638459
34	21.026527382240612	26.411201197424344	27.81433945535622	24.747931964978825
35	19.79660223371442	26.63795969650971	27.7381306681842	25.82730740159167
36	20.74908754920261	28.68625763800079	26.13836875838203	24.426286054414568
37	21.158772042333236	27.75207737433333	28.51441429437761	22.574736288955826
38	22.04936884928467	26.68229029819801	27.850326938187454	23.418013914329865
39	22.289326909546894	26.040243718689954	28.25042307084055	23.420006300922598
40	20.643117487301648	25.062355474144425	30.046933156675053	24.24759388187887
41	18.37217034407271	26.752397401429782	30.376299565286153	24.49913268921135
42	19.87505245580326	26.760989568610942	29.14450655432927	24.219451421256526
43	21.25191611554348	27.0265996062546	27.30727706750579	24.414207210696127
44	21.3988546267575	25.442901313356337	26.872936790290105	26.28530726959606
45	21.135361499868626	25.25623959444971	26.35130507548032	27.257093830201345
46	21.94987404381009	25.955193716012687	27.394319456775797	24.700612783401425
47	19.361514811526455	25.886082806077276	30.241564421952617	24.510837960443656
48	20.575874439796927	24.465386641296945	29.653436804610383	25.305302114295745
49	21.592863271224832	23.99543245373616	30.06361939438919	24.348084880649818
50	21.408941083883214	23.009450138657655	28.857478360813744	26.72413041664539
51	19.397626818519733	23.227865518885956	28.3123115793773	29.062196083217007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	337.0
2	572.0
3	1351.5
4	2131.0
5	1505.0
6	879.0
7	879.0
8	879.0
9	928.0
10	977.0
11	1115.0
12	1253.0
13	1257.5
14	1262.0
15	1274.0
16	1286.0
17	1332.0
18	1378.0
19	1475.5
20	1573.0
21	1701.0
22	1829.0
23	2206.0
24	2583.0
25	3257.0
26	5344.5
27	6758.0
28	7859.5
29	8961.0
30	11492.0
31	14023.0
32	15905.5
33	17788.0
34	19758.5
35	21729.0
36	24995.5
37	28262.0
38	30587.5
39	32913.0
40	36372.5
41	39832.0
42	45449.0
43	51066.0
44	57116.0
45	63166.0
46	74879.0
47	86592.0
48	91001.0
49	95410.0
50	90586.0
51	85762.0
52	74403.0
53	63044.0
54	54093.5
55	45143.0
56	40071.5
57	35000.0
58	30780.5
59	26561.0
60	24307.0
61	22053.0
62	17928.5
63	13804.0
64	11604.0
65	9404.0
66	7667.5
67	5931.0
68	4646.5
69	3362.0
70	2944.5
71	2527.0
72	1959.0
73	1391.0
74	1256.0
75	775.5
76	430.0
77	321.5
78	213.0
79	146.0
80	79.0
81	66.0
82	53.0
83	27.5
84	2.0
85	18.0
86	34.0
87	19.0
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	803057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.58055437844782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22275208056365	23.204767471435115
2	12.056714500343944	7.856288848077525
3	4.5417135379747995	4.439146346859618
4	2.2142206077240743	2.885621396633359
5	1.3903544693897125	2.2649259697634747
6	0.9542673814648059	1.8654336188035872
7	0.7018530596353296	1.6006733242590185
8	0.5632680443130178	1.4681268117905786
9	0.4427618491867338	1.2982883853717444
>10	5.55186134190428	41.4084974445249
>50	0.30374114534594143	6.0374759193904035
>100	0.050301079999896296	2.7551372472011164
>500	0.0030954510769166926	0.6308262084188688
>1k	0.0030954510769166926	2.2847910074707394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	2852	0.35514291015457183	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	2659	0.3311097468797358	TruSeq Adapter, Index 22 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	2596	0.3232647246708515	No Hit
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2407	0.2997296580441986	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	2260	0.28142460622346854	No Hit
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2145	0.26710432758820357	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2082	0.2592593053793193	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.1398406339774138	No Hit
TCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	955	0.11892057475372234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.490483240915651E-4	0.0	0.0	0.35501838599252605	0.0
2	2.490483240915651E-4	0.0	0.0	1.263297623954464	0.0
3	2.490483240915651E-4	0.0	0.0	1.8511761929726034	0.0
4	2.490483240915651E-4	0.0	0.0	2.538175994979186	0.0
5	2.490483240915651E-4	0.0	0.0	3.84368730986717	0.0
6	2.490483240915651E-4	0.0	0.0	4.655086749757489	0.0
7	2.490483240915651E-4	0.0	0.0	5.403227915328551	0.0
8	2.490483240915651E-4	0.0	0.0	6.63713783704021	0.0
9	2.490483240915651E-4	0.0	0.0	7.23946120885566	0.0
10	2.490483240915651E-4	0.0	0.0	8.634380872092516	0.0
11	2.490483240915651E-4	0.0	0.0	10.536736495665936	0.0
12	3.735724861373477E-4	0.0	0.0	12.663858231731995	0.0
13	4.980966481831302E-4	0.0	0.0	13.277015205645428	0.0
14	4.980966481831302E-4	0.0	0.0	13.482355548858923	0.0
15	4.980966481831302E-4	0.0	0.0	13.798522396293166	0.0
16	4.980966481831302E-4	0.0	0.0	14.438576589208488	0.0
17	4.980966481831302E-4	0.0	0.0	15.375496384440956	0.0
18	4.980966481831302E-4	0.0	0.0	16.42174839394962	0.0
19	4.980966481831302E-4	0.0	0.0	17.222812328390138	0.0
20	4.980966481831302E-4	0.0	0.0	18.018272675538597	0.0
21	4.980966481831302E-4	0.0	0.0	19.233753021267482	0.0
22	6.226208102289128E-4	0.0	0.0	20.558441057110517	0.0
23	6.226208102289128E-4	0.0	0.0	21.79222645466013	0.0
24	6.226208102289128E-4	0.0	0.0	22.68543826901453	0.0
25	6.226208102289128E-4	0.0	0.0	23.45948046029111	0.0
26	6.226208102289128E-4	0.0	0.0	24.138137143440627	0.0
27	6.226208102289128E-4	0.0	0.0	24.78964755926416	0.0
28	6.226208102289128E-4	0.0	0.0	25.52932108181611	0.0
29	6.226208102289128E-4	0.0	0.0	26.273601998363752	0.0
30	6.226208102289128E-4	0.0	0.0	27.091227646356362	0.0
31	6.226208102289128E-4	0.0	0.0	27.8798391645923	0.0
32	6.226208102289128E-4	0.0	0.0	28.633334869131332	0.0
33	6.226208102289128E-4	0.0	0.0	29.34499045522298	0.0
34	6.226208102289128E-4	0.0	0.0	30.036597651225257	0.0
35	6.226208102289128E-4	0.0	0.0	30.781750236907218	0.0
36	6.226208102289128E-4	0.0	0.0	31.51843517957007	0.0
37	6.226208102289128E-4	0.0	0.0	32.22237026761488	0.0
38	6.226208102289128E-4	0.0	0.0	32.93128632214152	0.0
39	6.226208102289128E-4	0.0	0.0	33.647424778066814	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTT	20	7.032015E-4	45.000004	43
TGGCACG	20	7.032015E-4	45.000004	32
GACGGTC	20	7.032015E-4	45.000004	9
CTCGAGT	20	7.032015E-4	45.000004	27
CAGTCGA	20	7.032015E-4	45.000004	28
ATCATCG	30	2.164681E-6	45.000004	23
TTATCCG	25	3.8897517E-5	45.0	1
TCGTGCG	25	3.8897517E-5	45.0	1
ACGCTCC	25	3.8897517E-5	45.0	25
GCGATAT	25	3.8897517E-5	45.0	9
CGACTAC	25	3.8897517E-5	45.0	45
CGCTTCA	25	3.8897517E-5	45.0	11
CCCGATC	25	3.8897517E-5	45.0	41
TCGCTTG	235	0.0	42.12766	1
GTATCGG	65	0.0	41.53846	2
ACGAGCG	60	3.6379788E-12	41.250004	42
CGTTTTT	865	0.0	41.098267	1
TATTGCG	50	1.0804797E-9	40.5	1
TATCCGG	100	0.0	40.5	2
ACCGGGT	45	1.9270374E-8	40.0	4
>>END_MODULE