##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933922.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636254
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3989035825315	33.0	31.0	34.0	31.0	34.0
2	32.61260754352821	34.0	31.0	34.0	31.0	34.0
3	32.681113203217585	34.0	31.0	34.0	31.0	34.0
4	36.060460130702516	37.0	35.0	37.0	35.0	37.0
5	36.09037899958193	37.0	35.0	37.0	35.0	37.0
6	35.69514219164044	37.0	35.0	37.0	35.0	37.0
7	36.02073071446309	37.0	35.0	37.0	35.0	37.0
8	36.02596132990912	37.0	35.0	37.0	35.0	37.0
9	37.86239300656656	39.0	38.0	39.0	35.0	39.0
10	37.572519779836355	39.0	37.0	39.0	35.0	39.0
11	37.5392013252569	39.0	37.0	39.0	35.0	39.0
12	37.293920352563596	39.0	37.0	39.0	35.0	39.0
13	37.18048295177712	39.0	37.0	39.0	34.0	39.0
14	38.383123721029655	40.0	38.0	41.0	34.0	41.0
15	38.48435844804119	40.0	38.0	41.0	34.0	41.0
16	38.56691509994436	40.0	38.0	41.0	35.0	41.0
17	38.530607273195926	40.0	38.0	41.0	35.0	41.0
18	38.499968566012946	40.0	38.0	41.0	35.0	41.0
19	38.52016175929739	40.0	37.0	41.0	34.0	41.0
20	38.4740512436857	40.0	37.0	41.0	34.0	41.0
21	38.366011058476644	40.0	37.0	41.0	34.0	41.0
22	38.370969769934646	40.0	37.0	41.0	34.0	41.0
23	38.31848601344746	40.0	37.0	41.0	34.0	41.0
24	38.28050904198638	40.0	37.0	41.0	34.0	41.0
25	38.17691205084762	40.0	37.0	41.0	34.0	41.0
26	38.10447242767825	40.0	37.0	41.0	34.0	41.0
27	38.08326863170998	40.0	37.0	41.0	34.0	41.0
28	37.99401654056399	40.0	36.0	41.0	34.0	41.0
29	37.98941617655842	40.0	36.0	41.0	34.0	41.0
30	37.92800988284553	40.0	36.0	41.0	34.0	41.0
31	37.77864029145593	40.0	36.0	41.0	33.0	41.0
32	37.70680891593609	40.0	36.0	41.0	33.0	41.0
33	37.69270291424494	40.0	36.0	41.0	33.0	41.0
34	37.643450571627056	40.0	36.0	41.0	33.0	41.0
35	37.612970606078704	40.0	36.0	41.0	33.0	41.0
36	37.52359120728514	40.0	36.0	41.0	33.0	41.0
37	37.41183552480613	40.0	35.0	41.0	33.0	41.0
38	37.369162315678956	40.0	35.0	41.0	33.0	41.0
39	37.373223586806525	39.0	35.0	41.0	33.0	41.0
40	37.22219270920104	39.0	35.0	41.0	33.0	41.0
41	37.105503462453676	39.0	35.0	41.0	32.0	41.0
42	37.110754509991295	39.0	35.0	41.0	32.0	41.0
43	37.02278806891587	39.0	35.0	41.0	32.0	41.0
44	36.907090564460105	39.0	35.0	41.0	32.0	41.0
45	36.89662304677063	39.0	35.0	41.0	32.0	41.0
46	36.796131733552954	39.0	35.0	41.0	31.0	41.0
47	36.68179060563863	39.0	35.0	41.0	31.0	41.0
48	36.64164154567201	39.0	35.0	40.0	31.0	41.0
49	36.63960619501017	39.0	35.0	40.0	31.0	41.0
50	36.553998874663264	39.0	35.0	40.0	31.0	41.0
51	35.62421454324845	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	13.0
10	22.0
11	10.0
12	12.0
13	5.0
14	8.0
15	15.0
16	21.0
17	41.0
18	65.0
19	140.0
20	274.0
21	420.0
22	715.0
23	1070.0
24	1565.0
25	2257.0
26	3173.0
27	3983.0
28	4851.0
29	5742.0
30	7266.0
31	9646.0
32	12877.0
33	18744.0
34	37035.0
35	55206.0
36	46301.0
37	73631.0
38	140333.0
39	210680.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.149861533287023	4.575531155796271	53.89687137526836	16.377735935648342
2	19.619365850745144	4.871167804053099	52.96721120810243	22.542255137099335
3	21.527566034948308	5.074702870237358	50.97995454645472	22.417776548359615
4	19.913273629713917	5.082089857195396	50.687618466838714	24.317018046251967
5	19.313984666501113	6.398545235079073	48.939888786553794	25.347581311866012
6	21.895500853432747	7.1939822775180975	52.551653899228924	18.358862969820226
7	83.2307537555756	2.6118499844401764	9.397347600172258	4.760048659811962
8	83.64851144354299	3.8929421268864317	7.339364467649712	5.1191819619208685
9	77.07032097244182	6.384242770968827	10.225475989148988	6.319960267440362
10	37.287938464826944	30.544719561684484	17.556038940423164	14.611303033065411
11	31.205461969590758	24.40471887013677	25.590251691934352	18.799567468338115
12	29.9341143631317	20.72725672451568	29.040288941209013	20.2983399711436
13	25.337836775878813	23.933523404175062	29.1774982947062	21.55114152523992
14	19.390054915175387	26.959830507941795	30.19045852756918	23.45965604931364
15	17.818669902271733	25.339565645166868	35.59946185014161	21.242302602419787
16	20.276650520075314	23.690853024106726	33.80049477095625	22.232001684861707
17	19.848991126185453	24.004092705114623	28.886105234701866	27.26081093399806
18	21.51263489109695	24.250535163629618	30.52020105178121	23.716628893492224
19	23.45839868983142	25.94891348423743	28.995338339719673	21.59734948621148
20	27.398020287495246	25.383101717238716	28.218133009772828	19.000744985493213
21	24.492576863956845	27.164308593737722	27.462931470764822	20.88018307154061
22	22.58406233988313	24.302715582141722	27.477548274745622	25.63567380322953
23	22.291097580526017	25.94246951689105	28.138919362393004	23.627513540189923
24	22.707440739075903	22.8125874257765	29.756197996397667	24.72377383874993
25	20.741087678820094	25.596852829216004	27.245879790146706	26.4161797018172
26	21.02273620283723	27.508353582059996	27.644148406139685	23.82476180896309
27	21.013777516526417	27.178925397718523	28.40123598437102	23.406061101384037
28	18.215209648976664	27.871416132550834	30.58165449647468	23.331719721997818
29	20.512091083120893	25.954728771842696	28.863158424151358	24.670021720885053
30	22.69125223574233	26.38364552521477	27.39471971885442	23.53038252018848
31	22.546970235157655	30.103072043554935	24.58310674667664	22.76685097461077
32	22.833491027168396	30.485623666020174	24.21768664715664	22.463198659654793
33	23.54358479475178	27.733892439183094	24.307273510264768	24.415249255800354
34	20.88191194082866	28.212160552232284	26.34969681919485	24.5562306877442
35	21.903516520131898	26.268754302526983	25.304359579664727	26.5233695976764
36	22.927164308593735	27.059161907037126	25.014381049077883	24.999292735291252
37	20.10564963049348	30.21073344921997	26.42859612670411	23.25502079358244
38	19.792252779550306	30.640750392138987	24.84715223794271	24.719844590367998
39	22.268622279781344	27.2765279275258	26.348910969518464	24.10593882317439
40	24.14507413705847	26.856412690529254	26.802346232793823	22.196166939618454
41	19.289623326533114	27.02301282192332	27.249494698658083	26.437869152885483
42	21.39271423048028	25.87017134666344	25.267110305003975	27.470004117852305
43	21.656916891681625	25.112455088690993	26.31606245304548	26.9145655665819
44	21.17110462173912	25.923923464528322	26.438812172497144	26.46615974123542
45	22.117581971979742	26.301288479129404	24.447311922596953	27.133817626293897
46	22.332276103568702	26.748122605123108	26.112213046990668	24.807388244317522
47	19.145655665818996	27.936484485755685	28.187013362587898	24.730846485837418
48	20.26046201674174	24.92809475461058	28.63887692651048	26.172566302137195
49	20.973699183030675	24.21674362754497	29.17812697444731	25.631430214977037
50	20.868552496330082	25.091865827169652	27.18756974415878	26.852011932341487
51	19.000273475687383	25.481175756851826	24.988448009757107	30.530102757703688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	357.5
2	646.0
3	1232.0
4	1818.0
5	1326.5
6	835.0
7	837.0
8	839.0
9	888.0
10	937.0
11	978.0
12	1019.0
13	988.5
14	958.0
15	937.5
16	917.0
17	1000.5
18	1084.0
19	1139.5
20	1195.0
21	1510.0
22	1825.0
23	2201.0
24	2577.0
25	2766.5
26	3802.0
27	4648.0
28	6054.5
29	7461.0
30	8526.5
31	9592.0
32	10800.0
33	12008.0
34	14042.0
35	16076.0
36	17564.0
37	19052.0
38	20251.0
39	21450.0
40	25408.5
41	29367.0
42	34385.5
43	39404.0
44	43272.0
45	47140.0
46	53354.5
47	59569.0
48	66383.5
49	73198.0
50	70510.0
51	67822.0
52	58203.0
53	48584.0
54	41784.5
55	34985.0
56	31951.0
57	28917.0
58	28251.0
59	27585.0
60	25222.5
61	22860.0
62	19623.0
63	16386.0
64	13526.5
65	10667.0
66	9237.0
67	7807.0
68	6340.5
69	4874.0
70	4292.0
71	3710.0
72	3082.5
73	2455.0
74	2042.0
75	1235.0
76	841.0
77	553.5
78	266.0
79	189.5
80	113.0
81	91.5
82	70.0
83	44.5
84	19.0
85	15.0
86	11.0
87	9.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	636254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.873960798595387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.58951504051886	18.83987492635496
2	13.138181590454124	7.324263172342918
3	5.582878921320089	4.66850844588542
4	2.911330769058624	3.246012789139386
5	1.6873127632791491	2.351604490930633
6	1.1113818062269358	1.8587167739425048
7	0.7658264582599967	1.4942631673246407
8	0.5844816764744702	1.3033455470037358
9	0.4523987161818071	1.1349129672167917
>10	5.268369054894547	37.754697243630176
>50	0.8283450596418216	14.092090549788086
>100	0.07369414668537483	3.5038346681002213
>500	0.003998907184477703	0.75971619712353
>1k	0.002285089819701545	1.6681590612169568
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	2923	0.4594077208159006	Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	2901	0.4559499822397973	Illumina Single End Adapter 1 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	2448	0.38475200155912576	Illumina Single End Adapter 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	2204	0.3464025373514351	Illumina Single End Adapter 1 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	879	0.13815237310885276	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.13579482407969143	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	674	0.10593253637698151	Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	644	0.1012174383186589	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5716993527742064E-4	0.0	0.0	0.48691245948944917	0.0
2	1.5716993527742064E-4	0.0	0.0	1.7845074451398342	0.0
3	1.5716993527742064E-4	0.0	0.0	2.534679546218963	0.0
4	1.5716993527742064E-4	0.0	0.0	3.330902438334376	0.0
5	1.5716993527742064E-4	0.0	0.0	4.603035894469818	0.0
6	1.5716993527742064E-4	0.0	0.0	5.394386518591632	0.0
7	1.5716993527742064E-4	0.0	0.0	6.120668789508592	0.0
8	1.5716993527742064E-4	0.0	0.0	7.280268571985402	0.0
9	1.5716993527742064E-4	0.0	0.0	7.8185756003105675	0.0
10	1.5716993527742064E-4	0.0	0.0	8.928195343369158	0.0
11	1.5716993527742064E-4	0.0	0.0	11.00959679624804	0.0
12	1.5716993527742064E-4	0.0	0.0	13.029701974368727	0.0
13	1.5716993527742064E-4	0.0	0.0	13.681328526028913	0.0
14	1.5716993527742064E-4	0.0	0.0	13.917397768815599	0.0
15	1.5716993527742064E-4	0.0	0.0	14.249497842056789	0.0
16	1.5716993527742064E-4	0.0	0.0	15.050750172101079	0.0
17	1.5716993527742064E-4	0.0	0.0	16.166813882506045	0.0
18	1.5716993527742064E-4	0.0	0.0	17.478711332266673	0.0
19	1.5716993527742064E-4	0.0	0.0	18.33638766907556	0.0
20	1.5716993527742064E-4	0.0	0.0	19.24844480349043	0.0
21	1.5716993527742064E-4	0.0	0.0	20.480657096065407	0.0
22	1.5716993527742064E-4	0.0	0.0	21.80528531058351	0.0
23	1.5716993527742064E-4	0.0	0.0	22.946967720438693	0.0
24	3.143398705548413E-4	0.0	0.0	23.82460463902781	0.0
25	3.143398705548413E-4	0.0	0.0	24.648960949557882	0.0
26	3.143398705548413E-4	0.0	0.0	25.350724710571566	0.0
27	3.143398705548413E-4	0.0	0.0	26.004394471390356	0.0
28	3.143398705548413E-4	0.0	0.0	26.688555199652967	0.0
29	3.143398705548413E-4	0.0	0.0	27.425839366039348	0.0
30	3.143398705548413E-4	0.0	0.0	28.258054173333292	0.0
31	3.143398705548413E-4	0.0	0.0	29.042646490238177	0.0
32	3.143398705548413E-4	0.0	0.0	29.82598144766084	0.0
33	3.143398705548413E-4	0.0	0.0	30.53340332634451	0.0
34	3.143398705548413E-4	0.0	0.0	31.252141440368156	0.0
35	4.71509805832262E-4	0.0	0.0	32.00860033885838	0.0
36	4.71509805832262E-4	0.0	0.0	32.7421124267981	0.0
37	4.71509805832262E-4	0.0	0.0	33.53990701826629	0.0
38	4.71509805832262E-4	0.0	0.0	34.26540343950686	0.0
39	4.71509805832262E-4	0.0	0.0	34.9835128737894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	25	3.8888345E-5	45.000004	19
CACGGCA	25	3.8888345E-5	45.000004	30
ATTACCG	25	3.8888345E-5	45.000004	12
TTCACCG	25	3.8888345E-5	45.000004	1
TTACTCG	25	3.8888345E-5	45.000004	1
CGCAGTC	25	3.8888345E-5	45.000004	37
ATAGTAA	25	3.8888345E-5	45.000004	34
AACTATC	25	3.8888345E-5	45.000004	37
CCCGGAC	25	3.8888345E-5	45.000004	4
TCGTTAT	20	7.0309103E-4	45.0	44
GCCCACG	20	7.0309103E-4	45.0	44
GATCGAC	20	7.0309103E-4	45.0	10
TAGCGCG	35	1.2107921E-7	45.0	1
CGATTGA	20	7.0309103E-4	45.0	10
CGTTCAT	40	6.8066583E-9	45.0	17
ACGGGAT	20	7.0309103E-4	45.0	5
AGACGTC	20	7.0309103E-4	45.0	16
ATATACG	20	7.0309103E-4	45.0	22
GGAACGT	20	7.0309103E-4	45.0	9
GCGATAT	40	6.8066583E-9	45.0	9
>>END_MODULE