##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933921.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475491
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.444309145704125	33.0	31.0	34.0	31.0	34.0
2	32.67748285456507	34.0	31.0	34.0	31.0	34.0
3	32.7306405378861	34.0	31.0	34.0	31.0	34.0
4	36.103856855334804	37.0	35.0	37.0	35.0	37.0
5	36.13228010624807	37.0	35.0	37.0	35.0	37.0
6	35.740872066979186	37.0	35.0	37.0	35.0	37.0
7	36.073038185791106	37.0	35.0	37.0	35.0	37.0
8	36.06803703960748	37.0	36.0	37.0	35.0	37.0
9	37.928129028730304	39.0	38.0	39.0	35.0	39.0
10	37.64206472888025	39.0	37.0	39.0	35.0	39.0
11	37.61568568069637	39.0	37.0	39.0	35.0	39.0
12	37.378709586511626	39.0	37.0	39.0	35.0	39.0
13	37.2830610884328	39.0	37.0	39.0	34.0	39.0
14	38.54150762054382	40.0	38.0	41.0	35.0	41.0
15	38.60653093328791	40.0	38.0	41.0	35.0	41.0
16	38.68705401364063	40.0	38.0	41.0	35.0	41.0
17	38.6604962028724	40.0	38.0	41.0	35.0	41.0
18	38.626005539537026	40.0	38.0	41.0	35.0	41.0
19	38.647560101032404	40.0	38.0	41.0	35.0	41.0
20	38.58498057797098	40.0	38.0	41.0	35.0	41.0
21	38.50375296272695	40.0	38.0	41.0	34.0	41.0
22	38.48227831862222	40.0	38.0	41.0	34.0	41.0
23	38.42465367378142	40.0	37.0	41.0	34.0	41.0
24	38.41235270488821	40.0	37.0	41.0	34.0	41.0
25	38.31330561461731	40.0	37.0	41.0	34.0	41.0
26	38.22938394207251	40.0	37.0	41.0	34.0	41.0
27	38.223779209280515	40.0	37.0	41.0	34.0	41.0
28	38.1348353596598	40.0	37.0	41.0	34.0	41.0
29	38.142412790147446	40.0	37.0	41.0	34.0	41.0
30	38.118254604188095	40.0	37.0	41.0	34.0	41.0
31	38.06043857822756	40.0	37.0	41.0	34.0	41.0
32	37.95204956560692	40.0	36.0	41.0	34.0	41.0
33	37.89832404819439	40.0	36.0	41.0	34.0	41.0
34	37.860182421959614	40.0	36.0	41.0	34.0	41.0
35	37.80982184731152	40.0	36.0	41.0	34.0	41.0
36	37.70391658306887	40.0	36.0	41.0	33.0	41.0
37	37.631488293153815	40.0	36.0	41.0	33.0	41.0
38	37.59586406472467	40.0	36.0	41.0	33.0	41.0
39	37.59834571001344	40.0	36.0	41.0	33.0	41.0
40	37.5337955923456	40.0	36.0	41.0	33.0	41.0
41	37.41509303015199	40.0	35.0	41.0	33.0	41.0
42	37.35692789137965	40.0	35.0	41.0	33.0	41.0
43	37.23770376305755	39.0	35.0	41.0	33.0	41.0
44	37.13123907708032	39.0	35.0	41.0	32.0	41.0
45	37.14422144688333	39.0	35.0	41.0	33.0	41.0
46	37.074382059807654	39.0	35.0	41.0	32.0	41.0
47	36.99574124431377	39.0	35.0	41.0	32.0	41.0
48	36.96104658132331	39.0	35.0	41.0	32.0	41.0
49	36.937296394674135	39.0	35.0	41.0	32.0	41.0
50	36.892832882220695	39.0	35.0	41.0	32.0	41.0
51	35.95106952602678	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	9.0
12	6.0
13	5.0
14	6.0
15	11.0
16	16.0
17	27.0
18	61.0
19	103.0
20	200.0
21	313.0
22	491.0
23	702.0
24	1106.0
25	1613.0
26	2276.0
27	2866.0
28	3136.0
29	3952.0
30	4909.0
31	6480.0
32	8827.0
33	12909.0
34	25564.0
35	39242.0
36	31562.0
37	51324.0
38	101294.0
39	176335.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.391227173595293	4.953195749236053	52.70467790136932	16.950899175799332
2	19.45588875499221	5.535120538559089	52.01044814728355	22.99854255916516
3	20.87168842312473	5.848270524573547	51.197183542906174	22.082857509395552
4	18.82348982420277	5.163714980935496	51.22010721548883	24.7926879793729
5	18.948833942177664	6.069725820257376	49.25182600722201	25.72961423034295
6	20.86348637513644	7.329896885535163	52.69794801584047	19.10866872348793
7	82.36412466271706	2.6002595212107065	9.8550761213146	5.180539694757629
8	82.74751782893894	3.987246866922823	7.57364492703332	5.69159037710493
9	76.57432001867544	5.975086805007876	10.726596297301105	6.723996879015585
10	36.74370282507976	30.706154270007215	17.601384673947564	14.948758230965463
11	30.749477908099205	25.177342999131426	24.902679545985098	19.170499546784274
12	28.333869621086414	21.867921790317798	30.040105911573512	19.758102677022276
13	24.566605887387986	25.358839599487688	29.647669461672248	20.42688505145208
14	19.239691182377793	28.53702804048867	29.03398802500994	23.189292752123595
15	17.031447493222796	25.672620512270473	35.30687226466958	21.98905972983716
16	18.469119289324087	25.44653842028556	33.082434788460766	23.001907501929583
17	18.73074358925826	25.32960665922173	30.023912124519708	25.9157376270003
18	20.300279079940527	25.25894286116877	31.66936913632435	22.77140892256636
19	22.99223329148186	26.145184661749642	28.444912732312496	22.417669314456003
20	26.24949788744687	24.634745978367626	29.729269323709595	19.38648681047591
21	24.124746840634206	27.57318224740321	27.93154865181465	20.37052226014793
22	22.35436632870023	25.74412554601454	27.487165897987552	24.414342227297677
23	21.098822059723528	27.00282444883289	27.46718655032377	24.431166941119812
24	22.107043035514867	23.906235869869253	30.057771861086753	23.92894923352913
25	19.883867412842722	27.338687798507227	27.51345451333464	25.26399027531541
26	20.250856483088008	29.10507244090845	26.966441005192525	23.677630070811016
27	21.726383885289103	28.127556567842504	27.917247645065835	22.22881190180256
28	19.032536893442778	27.151933475081545	31.91963675442858	21.895892877047093
29	20.822055517349437	25.137594612726634	29.649982859822792	24.390367010101137
30	21.832379582368542	25.853065568012855	29.087827109240767	23.226727740377843
31	22.39411471510502	27.244890018948837	27.700629454605867	22.660365811340277
32	24.857252818665337	27.43753299221226	25.97315196291833	21.73206222620407
33	23.84692875364623	26.8621277794953	25.703115306073094	23.587828160785378
34	21.010071694311776	25.386810686216982	29.233991810570547	24.369125808900694
35	22.058041056507903	25.244221236574404	27.703363470601968	24.994374236315725
36	22.68560288207348	28.672887604602398	26.39587289769943	22.245636615624694
37	22.711260570652232	26.376103858958423	30.091000670885464	20.821634899503884
38	21.62165004174632	26.579051969437906	27.94353626041292	23.855761728402854
39	22.368877644371818	25.826987261588545	28.260051189191802	23.54408390484783
40	23.73967120303013	24.286684711172242	29.210016593374004	22.763627492423623
41	20.723420632567176	25.35463342103215	28.66468555661411	25.257260389786556
42	23.15417116201989	25.188489372038585	27.484431881991455	24.172907583950064
43	23.318843048554022	25.31530565247292	27.60725229289303	23.75859900608003
44	21.989900965528264	24.749574650203684	27.681491342633198	25.579033041634858
45	22.03679985530746	24.274486793651196	26.768540308859684	26.92017304218166
46	22.369718880062926	25.19542956649022	28.62283408098155	23.812017472465303
47	20.665585678803595	25.032440151338303	31.52720030452732	22.774773865330786
48	21.493782216698108	23.518426216269077	30.511408207516023	24.47638335951679
49	21.42669367033235	23.367003791869877	31.497336437493033	23.70896610030474
50	21.786111619357673	23.028196117276668	29.665335411185488	25.520356852180164
51	20.08029594671613	23.274678174770923	27.096622228391283	29.548403650121664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	306.0
2	544.0
3	981.5
4	1419.0
5	1008.5
6	598.0
7	619.5
8	641.0
9	674.0
10	707.0
11	736.5
12	766.0
13	825.5
14	885.0
15	812.0
16	739.0
17	792.5
18	846.0
19	1097.5
20	1349.0
21	1370.5
22	1392.0
23	1590.5
24	1789.0
25	2135.0
26	3314.5
27	4148.0
28	5240.0
29	6332.0
30	7213.5
31	8095.0
32	9063.5
33	10032.0
34	11861.5
35	13691.0
36	14240.5
37	14790.0
38	15805.5
39	16821.0
40	20310.5
41	23800.0
42	27357.0
43	30914.0
44	32689.0
45	34464.0
46	41115.0
47	47766.0
48	50881.5
49	53997.0
50	52233.0
51	50469.0
52	42593.0
53	34717.0
54	29686.5
55	24656.0
56	22472.5
57	20289.0
58	18805.5
59	17322.0
60	16457.0
61	15592.0
62	13662.5
63	11733.0
64	9909.5
65	8086.0
66	6333.0
67	4580.0
68	3877.0
69	3174.0
70	2798.5
71	2423.0
72	1968.0
73	1513.0
74	1221.5
75	749.5
76	569.0
77	379.0
78	189.0
79	126.0
80	63.0
81	58.5
82	54.0
83	51.0
84	48.0
85	27.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.126819775446407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.44911622264364	21.224078277790625
2	12.263401686707025	7.389145848986561
3	4.802411825379054	4.340441866520021
4	2.3561770190864957	2.8393648165226963
5	1.3864430221766513	2.088455952902061
6	0.8803955537784144	1.5914110907871974
7	0.6594180115318041	1.390631731307133
8	0.5089358733165911	1.226609546613469
9	0.38351389486252546	1.0398648592712525
>10	5.842325837244512	43.582599944649864
>50	0.40454151222880824	7.439280050360593
>100	0.055580486027958	2.850614775693761
>500	0.004924853192350709	1.0578792732164146
>1k	0.0028142018242004055	1.939621965378363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	2853	0.6000113566818299	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	2390	0.5026383254362333	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	2056	0.4323951452288266	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	1852	0.38949212498238667	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	813	0.17098115421743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	800	0.1682471382213333	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	786	0.16530281330245997	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	742	0.15604922070028665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	721	0.15163273332197666	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	566	0.11903485029159332	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	563	0.11840392352326333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5251413801733367	0.0
2	0.0	0.0	0.0	2.046516127539743	0.0
3	0.0	0.0	0.0	2.850316830392163	0.0
4	0.0	0.0	0.0	3.6694700846072794	0.0
5	0.0	0.0	0.0	5.160560347093846	0.0
6	0.0	0.0	0.0	6.024719710783169	0.0
7	0.0	0.0	0.0	6.849130688067703	0.0
8	0.0	0.0	0.0	8.21214281658328	0.0
9	0.0	0.0	0.0	8.90931689558793	0.0
10	0.0	0.0	0.0	10.323854710183788	0.0
11	0.0	0.0	0.0	12.262061742493549	0.0
12	0.0	0.0	0.0	14.262099598099649	0.0
13	0.0	0.0	0.0	14.848020256955442	0.0
14	0.0	0.0	0.0	15.058539488654885	0.0
15	0.0	0.0	0.0	15.390406968796466	0.0
16	0.0	0.0	0.0	16.160768552927394	0.0
17	0.0	0.0	0.0	17.267414104578215	0.0
18	0.0	0.0	0.0	18.485102767455114	0.0
19	0.0	0.0	0.0	19.22854480947063	0.0
20	0.0	0.0	0.0	20.10048560330269	0.0
21	0.0	0.0	0.0	21.191778603590816	0.0
22	0.0	0.0	0.0	22.34153748441085	0.0
23	0.0	0.0	0.0	23.377939855854265	0.0
24	0.0	0.0	0.0	24.163233373502337	0.0
25	0.0	0.0	0.0	24.866506411267512	0.0
26	0.0	0.0	0.0	25.46904147502266	0.0
27	0.0	0.0	0.0	26.07052499416393	0.0
28	0.0	0.0	0.0	26.689884771741212	0.0
29	0.0	0.0	0.0	27.31197856531459	0.0
30	0.0	0.0	0.0	28.072455630075016	0.0
31	0.0	0.0	0.0	28.770260635847997	0.0
32	0.0	0.0	0.0	29.437360538895582	0.0
33	0.0	0.0	0.0	30.048728577407353	0.0
34	0.0	0.0	0.0	30.63759356118202	0.0
35	0.0	0.0	0.0	31.331192388499467	0.0
36	0.0	0.0	0.0	31.964011937134458	0.0
37	0.0	0.0	0.0	32.612604654977694	0.0
38	0.0	0.0	0.0	33.20420365474846	0.0
39	0.0	0.0	0.0	33.79979852405198	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	60	0.0	45.000004	4
ATCGGTT	30	2.1628057E-6	45.000004	32
ATACGAC	30	2.1628057E-6	45.000004	23
TCGGTTA	30	2.1628057E-6	45.000004	33
GCGAGAC	35	1.209919E-7	45.0	21
CTCGTAG	20	7.0291094E-4	45.0	1
AGTCACG	25	3.887344E-5	45.0	15
CGAAAGG	20	7.0291094E-4	45.0	13
GCGAAGG	25	3.887344E-5	45.0	3
ACCGAGC	20	7.0291094E-4	45.0	33
CACGCAA	20	7.0291094E-4	45.0	15
ATACTCG	20	7.0291094E-4	45.0	44
CGGATGG	25	3.887344E-5	45.0	2
GGGCGTA	20	7.0291094E-4	45.0	7
CGCTAAC	20	7.0291094E-4	45.0	14
GTCACGA	25	3.887344E-5	45.0	16
AATCTAG	20	7.0291094E-4	45.0	33
ATAAGCG	20	7.0291094E-4	45.0	33
TAGGGCG	25	3.887344E-5	45.0	5
CCGGTTA	25	3.887344E-5	45.0	31
>>END_MODULE