FastQCFastQC Report
Sat 14 Jan 2017
SRR2933920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773578
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC54110.6994769758188574No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC47620.6155811049435223TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG44170.5709831458495459No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC42550.5500414954923744TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT39290.507899655884733Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC37210.4810116109816981TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC30240.39091080666720096TruSeq Adapter, Index 13 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG12100.1564160304455401Illumina PCR Primer Index 4 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT11620.15021109700637816RNA PCR Primer, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC11250.1454281274803575No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT10920.1411622357409337No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT10430.13482803285512257No Hit
CGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG10160.13133775779559398Illumina PCR Primer Index 4 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.12319378265669396No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA9440.12203035763685109No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG8700.11246441858480981TruSeq Adapter, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT351.2112469E-745.0000049
AACCGTG207.031855E-445.011
TCCGCGA207.031855E-445.029
CGCGGAT253.8896185E-545.04
TATTGCG406.8102963E-945.01
TCTCGAT207.031855E-445.044
ACAGCGT207.031855E-445.028
GTCGTAC207.031855E-445.09
CTCGATA207.031855E-445.045
CCGAATG253.8896185E-545.022
CCGCGAG253.8896185E-545.030
CCGTTCG406.8102963E-945.01
TACGTCC207.031855E-445.035
CGCTTAC207.031855E-445.03
CTTACGA253.8896185E-545.019
CACGGGA1150.044.9999964
CGTAGTT302.1645792E-644.99999626
CGTTCGG800.042.18752
CGTAGGG650.041.5384643
TTACACG1250.041.39999834
Produced by FastQC (version 0.11.5)