##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933920.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773578
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.371617341754806	33.0	31.0	34.0	31.0	34.0
2	32.590319269679334	34.0	31.0	34.0	31.0	34.0
3	32.65715286629144	34.0	31.0	34.0	31.0	34.0
4	36.04528567255015	37.0	35.0	37.0	35.0	37.0
5	36.08471026838922	37.0	35.0	37.0	35.0	37.0
6	35.612083073717194	37.0	35.0	37.0	33.0	37.0
7	35.99594740284755	37.0	35.0	37.0	35.0	37.0
8	35.96233088324642	37.0	35.0	37.0	35.0	37.0
9	37.83698088621962	39.0	38.0	39.0	35.0	39.0
10	37.529836680981106	39.0	37.0	39.0	35.0	39.0
11	37.48281233437352	39.0	37.0	39.0	35.0	39.0
12	37.31542520599086	39.0	37.0	39.0	35.0	39.0
13	37.22603796902187	39.0	37.0	39.0	34.0	39.0
14	38.44657164500541	40.0	38.0	41.0	34.0	41.0
15	38.504290452934285	40.0	38.0	41.0	34.0	41.0
16	38.59342432178785	40.0	38.0	41.0	35.0	41.0
17	38.541750153184296	40.0	38.0	41.0	34.0	41.0
18	38.50344761614213	40.0	38.0	41.0	34.0	41.0
19	38.51329665528234	40.0	38.0	41.0	34.0	41.0
20	38.428065431023114	40.0	37.0	41.0	34.0	41.0
21	38.38936603677974	40.0	37.0	41.0	34.0	41.0
22	38.40021820682595	40.0	37.0	41.0	34.0	41.0
23	38.325443588106175	40.0	37.0	41.0	34.0	41.0
24	38.285235619420405	40.0	37.0	41.0	34.0	41.0
25	38.19908528939551	40.0	37.0	41.0	34.0	41.0
26	38.15016973078345	40.0	37.0	41.0	34.0	41.0
27	38.12580502547901	40.0	37.0	41.0	34.0	41.0
28	38.064272768873984	40.0	37.0	41.0	34.0	41.0
29	38.03052956521514	40.0	37.0	41.0	34.0	41.0
30	37.97167318615576	40.0	37.0	41.0	34.0	41.0
31	37.921772594360235	40.0	37.0	41.0	34.0	41.0
32	37.76075974239185	40.0	36.0	41.0	33.0	41.0
33	37.72830406242163	40.0	36.0	41.0	33.0	41.0
34	37.715829560819984	40.0	36.0	41.0	33.0	41.0
35	37.64411992068027	40.0	36.0	41.0	33.0	41.0
36	37.55326676818627	40.0	36.0	41.0	33.0	41.0
37	37.494769758188575	40.0	36.0	41.0	33.0	41.0
38	37.432026246868446	40.0	36.0	41.0	33.0	41.0
39	37.3650013314753	40.0	35.0	41.0	33.0	41.0
40	37.24089490652526	39.0	35.0	41.0	32.0	41.0
41	37.1808647608903	39.0	35.0	41.0	32.0	41.0
42	37.15870797773463	39.0	35.0	41.0	32.0	41.0
43	37.078983631902666	39.0	35.0	41.0	32.0	41.0
44	37.00523411989483	39.0	35.0	41.0	32.0	41.0
45	37.01336129000566	39.0	35.0	41.0	32.0	41.0
46	36.90502444485236	39.0	35.0	41.0	32.0	41.0
47	36.80064195207206	39.0	35.0	41.0	31.0	41.0
48	36.74346866120805	39.0	35.0	41.0	31.0	41.0
49	36.754459149562166	39.0	35.0	41.0	31.0	41.0
50	36.68984898743243	39.0	35.0	41.0	31.0	41.0
51	35.75683512199158	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	13.0
11	8.0
12	9.0
13	10.0
14	6.0
15	11.0
16	20.0
17	52.0
18	94.0
19	220.0
20	391.0
21	759.0
22	1059.0
23	1517.0
24	2105.0
25	2960.0
26	3927.0
27	4903.0
28	5868.0
29	7121.0
30	8891.0
31	11891.0
32	15968.0
33	22581.0
34	42558.0
35	60539.0
36	55584.0
37	87033.0
38	167583.0
39	269727.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.42021877561151	4.75310828384468	52.572461988319205	16.2542109522246
2	19.509603427191568	7.936368407581394	51.10939039114349	21.444637774083546
3	20.01452988580337	8.286817877447394	49.937692126715085	21.76096011003415
4	19.04668953874076	4.715103066529814	51.55834317935619	24.67986421537324
5	20.805788168743163	5.447414481797569	47.99322111021772	25.75357623924155
6	20.911530576102216	8.980865536506984	51.4506617302974	18.656942157093404
7	80.66335909242507	2.2672568247804357	11.790407689980842	5.278976392813653
8	80.52982375403643	5.4611170431423846	8.325857250335455	5.683201952485723
9	74.54387275749828	5.697938669403732	10.68605880725667	9.072129765841325
10	38.36613760991135	26.073518119698335	19.82101352416951	15.739330746220809
11	30.22927229057703	23.555090760078492	25.47642254562565	20.739214403718822
12	25.44824180625612	21.32803673320596	30.502806439686754	22.720915020851162
13	24.047219543472025	23.688496829020476	32.389881821871874	19.87440180563563
14	20.30797152969707	26.758129109152534	30.135293402863063	22.798605958287336
15	17.8265152318189	25.282906183991788	35.32507387748876	21.56550470670055
16	19.072543428070603	25.583845455791142	30.95783489189196	24.385776224246296
17	19.79464255705307	25.550364669108998	29.49372913914305	25.161263634694887
18	20.249024662025032	24.747084327630827	31.14721463123305	23.856676379111093
19	21.82494848612551	25.940112050756355	28.866901592341044	23.36803787077709
20	23.96771883378276	25.934424195103787	30.86566577643108	19.232191194682372
21	23.607832694311366	27.047951208540056	29.081488873778728	20.262727223369847
22	22.356892259086997	24.06079283537019	29.066235079074122	24.516079826468694
23	21.2485360235167	26.541214977675164	28.268771862695164	23.941477136112972
24	22.01743069218618	24.041531687819457	29.224719420666045	24.716318199328317
25	19.822306218636	27.73488904803394	27.08828327589461	25.354521457435446
26	19.78804981527396	26.193221627295504	28.843633091944188	25.17509546548635
27	20.07011574786253	26.211448619273042	29.369113392573208	24.34932224029122
28	17.2480344580637	25.38683881909775	31.66752415399611	25.69760256884244
29	18.53956549953592	25.288723309091004	29.507690239381162	26.664020951991912
30	20.3025422129378	26.584908050642596	29.69396751200267	23.41858222441693
31	22.07935075713115	27.119954290323665	25.891894547156202	24.908800405388984
32	21.08358820959231	28.724188123240317	26.56176881969239	23.63045484747498
33	20.796997846371017	25.613318889627163	27.05247563917278	26.53720762482904
34	20.523851505601247	25.083960505598657	27.63069787403468	26.76149011476541
35	19.037640677475316	24.314548759142582	29.198219184102957	27.44959137927914
36	20.64407209098501	24.650390781537222	27.987998624573084	26.717538502904688
37	19.470693323750158	25.568203852746592	31.38015817409492	23.580944649408334
38	18.869848935724644	27.987093738446546	27.11853232641052	26.024524999418286
39	21.520518939266626	27.509572402524373	27.277921554128998	23.691987104080003
40	22.105850993694236	25.1182169089607	29.293232227390124	23.48269986995494
41	20.678974841580295	24.084190605213696	28.083399476200206	27.15343507700581
42	20.185941172060218	22.26019871299339	29.990123814276	27.563736300670392
43	20.8571081390629	23.508036681498183	27.79293102957944	27.84192414985948
44	19.955195209791384	23.822937053535647	28.550837795283734	27.67102994138923
45	20.65467218561024	23.64816476166592	27.04200481399419	28.655158238729644
46	22.01575018937974	24.193164748738976	29.05692767891538	24.734157382965908
47	18.65215918756738	23.744082691079633	32.694957715964	24.908800405388984
48	19.134204954122275	23.962030978130194	30.10284677175411	26.800917295993422
49	20.081749998060957	21.86036831450739	32.32809102637355	25.729790661058093
50	20.920967245707608	22.802871850026758	29.314044608300648	26.962116295964982
51	19.56790394763036	22.12614629681816	27.167396177243923	31.138553578307555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	367.5
2	661.0
3	1346.5
4	2032.0
5	1492.0
6	952.0
7	940.5
8	929.0
9	1047.5
10	1166.0
11	1177.5
12	1189.0
13	1171.5
14	1154.0
15	1240.0
16	1326.0
17	1261.5
18	1197.0
19	1370.5
20	1544.0
21	1726.5
22	1909.0
23	2283.5
24	2658.0
25	3564.5
26	5350.0
27	6229.0
28	6836.5
29	7444.0
30	9686.5
31	11929.0
32	13531.0
33	15133.0
34	17584.5
35	20036.0
36	21490.5
37	22945.0
38	25459.0
39	27973.0
40	32781.5
41	37590.0
42	41712.0
43	45834.0
44	51110.0
45	56386.0
46	72805.5
47	89225.0
48	90719.0
49	92213.0
50	89305.0
51	86397.0
52	72660.0
53	58923.0
54	50490.0
55	42057.0
56	37244.5
57	32432.0
58	28787.5
59	25143.0
60	23740.5
61	22338.0
62	19567.5
63	16797.0
64	14651.5
65	12506.0
66	10375.0
67	8244.0
68	6982.5
69	5721.0
70	4569.5
71	3418.0
72	2863.0
73	2308.0
74	1845.0
75	1124.0
76	866.0
77	622.5
78	379.0
79	322.5
80	266.0
81	214.5
82	163.0
83	95.0
84	27.0
85	15.0
86	3.0
87	4.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.440061829684872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96540327371011	19.613803332134673
2	12.747663069420167	7.250886517565999
3	5.282808717020232	4.507302196393608
4	2.7108097355232164	3.0838238594716785
5	1.5916733484437215	2.2633644221200497
6	1.0083659336475224	1.720679369993007
7	0.6931692490627082	1.3799643411245757
8	0.5146846482405142	1.1710130574999866
9	0.39366061664752106	1.0076159049630635
>10	5.498447185434176	40.57003051099067
>50	0.5220245294218859	8.896858284386031
>100	0.062091023308452065	3.1197081574640464
>500	0.003219534541919737	0.6841121032929545
>1k	0.005519202071862406	4.0230500667070945
>5k	4.599335059885338E-4	0.7077878758925767
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	5411	0.6994769758188574	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	4762	0.6155811049435223	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	4417	0.5709831458495459	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	4255	0.5500414954923744	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	3929	0.507899655884733	Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	3721	0.4810116109816981	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	3024	0.39091080666720096	TruSeq Adapter, Index 13 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1210	0.1564160304455401	Illumina PCR Primer Index 4 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1162	0.15021109700637816	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	1125	0.1454281274803575	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	1092	0.1411622357409337	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT	1043	0.13482803285512257	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1016	0.13133775779559398	Illumina PCR Primer Index 4 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.12319378265669396	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	944	0.12203035763685109	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	870	0.11246441858480981	TruSeq Adapter, Index 13 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.585388932984134E-4	0.0	0.0	0.5437072926065633	0.0
2	2.585388932984134E-4	0.0	0.0	2.1201481944936384	0.0
3	2.585388932984134E-4	0.0	0.0	2.962855717199817	0.0
4	2.585388932984134E-4	0.0	0.0	3.9771037956094926	0.0
5	2.585388932984134E-4	0.0	0.0	5.952987287642617	0.0
6	2.585388932984134E-4	0.0	0.0	6.790394763036177	0.0
7	2.585388932984134E-4	0.0	0.0	7.683517369935546	0.0
8	2.585388932984134E-4	0.0	0.0	8.97840941702065	0.0
9	2.585388932984134E-4	0.0	0.0	9.49018715630486	0.0
10	2.585388932984134E-4	0.0	0.0	11.137596984402348	0.0
11	2.585388932984134E-4	0.0	0.0	13.057894614376314	0.0
12	2.585388932984134E-4	0.0	0.0	15.67702287293589	0.0
13	2.585388932984134E-4	0.0	0.0	16.326472572901505	0.0
14	2.585388932984134E-4	0.0	0.0	16.58488219675327	0.0
15	2.585388932984134E-4	0.0	0.0	17.065893807734966	0.0
16	2.585388932984134E-4	0.0	0.0	17.923208777912507	0.0
17	2.585388932984134E-4	0.0	0.0	19.005323315813015	0.0
18	2.585388932984134E-4	0.0	0.0	20.17120445514221	0.0
19	2.585388932984134E-4	0.0	0.0	21.127410552006392	0.0
20	2.585388932984134E-4	0.0	0.0	22.021567314478954	0.0
21	2.585388932984134E-4	0.0	0.0	23.217826773770714	1.292694466492067E-4
22	2.585388932984134E-4	0.0	0.0	24.442525511325297	1.292694466492067E-4
23	2.585388932984134E-4	0.0	0.0	25.59560897543622	1.292694466492067E-4
24	2.585388932984134E-4	0.0	0.0	26.48485349893611	1.292694466492067E-4
25	2.585388932984134E-4	0.0	0.0	27.265382417804023	1.292694466492067E-4
26	2.585388932984134E-4	0.0	0.0	27.960205693543507	1.292694466492067E-4
27	2.585388932984134E-4	0.0	0.0	28.648177688610588	1.292694466492067E-4
28	2.585388932984134E-4	0.0	0.0	29.35321325063536	1.292694466492067E-4
29	2.585388932984134E-4	0.0	0.0	30.054629268153956	1.292694466492067E-4
30	2.585388932984134E-4	0.0	0.0	30.869802398723852	1.292694466492067E-4
31	2.585388932984134E-4	0.0	0.0	31.620340805969146	1.292694466492067E-4
32	2.585388932984134E-4	0.0	0.0	32.327703218033605	1.292694466492067E-4
33	2.585388932984134E-4	0.0	0.0	33.00998735744812	1.292694466492067E-4
34	2.585388932984134E-4	0.0	0.0	33.69925204698169	1.292694466492067E-4
35	2.585388932984134E-4	0.0	0.0	34.41941213426442	1.292694466492067E-4
36	2.585388932984134E-4	0.0	0.0	35.112684176644116	1.292694466492067E-4
37	2.585388932984134E-4	0.0	0.0	35.76717538502905	1.292694466492067E-4
38	2.585388932984134E-4	0.0	0.0	36.41675435444131	1.292694466492067E-4
39	2.585388932984134E-4	0.0	0.0	37.11623133026017	1.292694466492067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	35	1.2112469E-7	45.000004	9
AACCGTG	20	7.031855E-4	45.0	11
TCCGCGA	20	7.031855E-4	45.0	29
CGCGGAT	25	3.8896185E-5	45.0	4
TATTGCG	40	6.8102963E-9	45.0	1
TCTCGAT	20	7.031855E-4	45.0	44
ACAGCGT	20	7.031855E-4	45.0	28
GTCGTAC	20	7.031855E-4	45.0	9
CTCGATA	20	7.031855E-4	45.0	45
CCGAATG	25	3.8896185E-5	45.0	22
CCGCGAG	25	3.8896185E-5	45.0	30
CCGTTCG	40	6.8102963E-9	45.0	1
TACGTCC	20	7.031855E-4	45.0	35
CGCTTAC	20	7.031855E-4	45.0	3
CTTACGA	25	3.8896185E-5	45.0	19
CACGGGA	115	0.0	44.999996	4
CGTAGTT	30	2.1645792E-6	44.999996	26
CGTTCGG	80	0.0	42.1875	2
CGTAGGG	65	0.0	41.538464	3
TTACACG	125	0.0	41.399998	34
>>END_MODULE