##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933919.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369011 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45684877623335 33.0 31.0 34.0 31.0 34.0 2 32.68134733760357 34.0 31.0 34.0 31.0 34.0 3 32.735796133120914 34.0 31.0 34.0 31.0 34.0 4 36.10384284713563 37.0 35.0 37.0 35.0 37.0 5 36.1249705079068 37.0 35.0 37.0 35.0 37.0 6 35.67652706954144 37.0 35.0 37.0 35.0 37.0 7 36.02702827077357 37.0 35.0 37.0 35.0 37.0 8 36.001146813283455 37.0 36.0 37.0 35.0 37.0 9 37.846852216673206 39.0 38.0 39.0 35.0 39.0 10 37.61606517405631 39.0 37.0 39.0 35.0 39.0 11 37.60696882640096 39.0 37.0 39.0 35.0 39.0 12 37.461839240152194 39.0 37.0 39.0 35.0 39.0 13 37.400272897734204 39.0 37.0 39.0 35.0 39.0 14 38.68136779032455 40.0 38.0 41.0 35.0 41.0 15 38.72821620863529 40.0 38.0 41.0 35.0 41.0 16 38.81367571188252 40.0 38.0 41.0 35.0 41.0 17 38.74658567389159 40.0 38.0 41.0 35.0 41.0 18 38.692823505435676 40.0 38.0 41.0 35.0 41.0 19 38.694381564501676 40.0 38.0 41.0 35.0 41.0 20 38.61562105782934 40.0 38.0 41.0 35.0 41.0 21 38.567562276709246 40.0 38.0 41.0 34.0 41.0 22 38.548872872460485 40.0 38.0 41.0 34.0 41.0 23 38.477796014787316 40.0 38.0 41.0 34.0 41.0 24 38.445935058228166 40.0 38.0 41.0 34.0 41.0 25 38.37091082540608 40.0 38.0 41.0 34.0 41.0 26 38.28644766185224 40.0 37.0 41.0 34.0 41.0 27 38.254985533352176 40.0 37.0 41.0 34.0 41.0 28 38.1381990356542 40.0 37.0 41.0 34.0 41.0 29 38.1168792653967 40.0 37.0 41.0 34.0 41.0 30 38.059722675712614 40.0 37.0 41.0 34.0 41.0 31 37.98485987329539 40.0 37.0 41.0 34.0 41.0 32 37.86762414619021 40.0 37.0 41.0 33.0 41.0 33 37.77975049141315 40.0 37.0 41.0 33.0 41.0 34 37.70836392110801 40.0 37.0 41.0 33.0 41.0 35 37.64412411587635 40.0 36.0 41.0 33.0 41.0 36 37.51417994450008 40.0 36.0 41.0 33.0 41.0 37 37.436520232489 40.0 36.0 41.0 33.0 41.0 38 37.34282631768481 40.0 36.0 41.0 32.0 41.0 39 37.32463289191979 40.0 36.0 41.0 32.0 41.0 40 37.17043836755147 39.0 35.0 41.0 32.0 41.0 41 36.99790943973423 39.0 35.0 41.0 32.0 41.0 42 36.95096314054452 39.0 35.0 41.0 31.0 41.0 43 36.87353425209878 39.0 35.0 41.0 31.0 41.0 44 36.79362327987138 39.0 35.0 41.0 31.0 41.0 45 36.80433100975814 39.0 35.0 41.0 31.0 41.0 46 36.722122028237905 39.0 35.0 41.0 31.0 41.0 47 36.59446929206558 39.0 35.0 41.0 31.0 41.0 48 36.49589667285362 39.0 35.0 41.0 31.0 41.0 49 36.43485771845515 39.0 35.0 40.0 31.0 41.0 50 36.330935251798564 38.0 35.0 40.0 31.0 41.0 51 35.29393627954779 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 23.0 10 27.0 11 21.0 12 17.0 13 28.0 14 21.0 15 43.0 16 67.0 17 126.0 18 241.0 19 450.0 20 959.0 21 1454.0 22 2262.0 23 3079.0 24 4205.0 25 5900.0 26 7872.0 27 9581.0 28 11222.0 29 13160.0 30 16105.0 31 20801.0 32 27338.0 33 38121.0 34 69825.0 35 92453.0 36 98318.0 37 157153.0 38 295275.0 39 492535.0 40 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.670429236872454 4.631518665664483 52.893950450361615 16.804101647101447 2 19.82935126160418 7.0305497910535415 52.08665233515289 21.053446612189383 3 20.841687904626042 6.968315082932132 50.476146648931234 21.71385036351059 4 19.324242098858228 5.020047318830893 50.942687823545604 24.71302275876527 5 20.56594139857167 5.874532783155139 47.524161602792084 26.035364215481106 6 21.88645671948582 8.455081807231645 50.688197538222845 18.970263935059688 7 82.63030757240081 2.5198482700285094 10.218690719066538 4.631153438504146 8 83.32409308617682 5.050142036842655 7.0078326616805855 4.61793221529995 9 76.83488299217464 6.296735380504613 9.175748040008443 7.6926335873123 10 36.28232351675772 33.46276983895674 15.738880111262802 14.516026533022744 11 31.629475584929555 25.18555365880917 24.2406379495855 18.944332806675767 12 30.6856555571869 20.16294974985592 27.83352361668387 21.31787107627331 13 26.800880343547277 21.055053611694866 29.767401430667835 22.376664614090025 14 20.729636211834677 25.240702960020045 28.993412032481842 25.03624879566344 15 18.777423994401797 25.175911661776272 33.53588831645619 22.510776027365743 16 21.127003362281236 23.643053269842245 31.971474297869047 23.25846907000747 17 21.359580017983784 23.9513049931666 30.094133648305238 24.59498134054438 18 21.10596627784583 25.413528452291473 28.94987695496968 24.530628314893015 19 24.24348672143613 26.439232409381663 26.955152296073585 22.362128573108617 20 27.954413806755383 25.014042984314955 27.798169627563258 19.233373581366404 21 26.334412214364967 26.1166637813721 26.29803558919541 21.25088841506752 22 23.51697685409394 24.72244561950196 27.014757368640574 24.745820157763525 23 24.125664439511443 26.099352013972133 26.74164049814063 23.033343048375798 24 24.34239023645537 22.959274980259472 27.44630978129467 25.252025001990486 25 23.38805166649501 26.574877776730794 24.909076698434124 25.12799385834007 26 23.39418748278867 26.787513029478944 26.21490988750273 23.603389600229654 27 24.388481904089886 26.80358302453377 26.090075244099577 22.717859827276772 28 21.888282855287503 27.753758004866285 28.049445913875054 22.308513225971158 29 24.280228573766024 25.209950833119677 27.51614121435109 22.99367937876321 30 24.571533756850748 25.869039766663672 26.94507202644829 22.61435445003729 31 24.09469317631487 27.456682232648237 24.93179382780708 23.516830763229805 32 25.70271531784624 27.460699731411946 25.872692038267044 20.963892912474773 33 25.7428903054833 27.056758492079318 25.245670049400626 21.954681153036752 34 25.60059780381604 25.63551352034425 26.52747129131906 22.236417384520653 35 25.160864302770392 23.669641807114772 26.99766473753681 24.171829152578027 36 24.760502289609068 28.03695514499153 24.843554945869684 22.358987619529717 37 25.034860932454155 27.390064798602786 27.175238182892613 20.399836086050442 38 22.98827401679022 27.04295290541858 26.796132390462894 23.172640687328297 39 25.01228989394534 24.74684279381247 27.798242672995322 22.442624639246873 40 25.06780442231655 24.126467939264185 29.86688930914361 20.93883832927566 41 23.500687722742914 25.374376100703355 28.64345136744701 22.481484809106718 42 23.781401318177867 24.914920332999515 28.20912322837435 23.094555120448266 43 24.996146853458445 25.103888865757835 27.034187453570496 22.86577682721322 44 23.841152481608987 24.91141415226028 27.157342052036103 24.09009131409463 45 23.348826269474827 23.55269607037489 26.76165494652709 26.336822713623192 46 23.723987608572905 25.88087312665859 26.64164130164038 23.753497963128126 47 22.480023900465373 25.61476861763711 29.586833122597263 22.318374359300254 48 23.013547736285535 23.834432301858786 28.718176844451943 24.433843117403732 49 22.316329087202366 24.30075434017696 29.997202359951817 23.385714212668855 50 22.37819856816344 23.87935524258023 28.350100912264402 25.39234527699193 51 20.94417064581658 23.580818561720836 27.595760735304538 27.87925005715805 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 846.0 2 1505.0 3 3663.5 4 5822.0 5 4055.5 6 2289.0 7 2269.5 8 2250.0 9 2230.5 10 2211.0 11 2219.5 12 2228.0 13 2251.5 14 2275.0 15 2255.5 16 2236.0 17 2217.0 18 2198.0 19 2436.5 20 2675.0 21 2961.0 22 3247.0 23 4111.0 24 4975.0 25 6273.0 26 9380.0 27 11189.0 28 13494.0 29 15799.0 30 17985.0 31 20171.0 32 24297.5 33 28424.0 34 32369.0 35 36314.0 36 38271.0 37 40228.0 38 44962.0 39 49696.0 40 54777.0 41 59858.0 42 63588.5 43 67319.0 44 74892.5 45 82466.0 46 103742.0 47 125018.0 48 125959.0 49 126900.0 50 126068.5 51 125237.0 52 110731.0 53 96225.0 54 88020.5 55 79816.0 56 75887.5 57 71959.0 58 72558.5 59 73158.0 60 72044.0 61 70930.0 62 61436.0 63 51942.0 64 43632.0 65 35322.0 66 28629.0 67 21936.0 68 17839.5 69 13743.0 70 11558.0 71 9373.0 72 7859.5 73 6346.0 74 5186.5 75 3052.0 76 2077.0 77 1544.0 78 1011.0 79 697.0 80 383.0 81 308.0 82 233.0 83 181.5 84 130.0 85 86.0 86 42.0 87 49.5 88 57.0 89 33.0 90 9.0 91 5.0 92 1.0 93 0.5 94 0.0 95 1.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1369011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.638410733034757 #Duplication Level Percentage of deduplicated Percentage of total 1 71.70797936066285 19.818945864059785 2 10.761189605165274 5.948443565672439 3 3.983049171668677 3.3025544692935824 4 1.8765270328765207 2.0745689954513717 5 1.2064140354869706 1.667168331344343 6 0.841826800676974 1.3960052929912075 7 0.6519833101168082 1.2613847761264345 8 0.520403467013282 1.1506499814566713 9 0.44488472085636416 1.1066315979491737 >10 7.620226172910238 49.78175553934106 >50 0.30948484630980166 5.28729726716909 >100 0.06962882564344564 3.4738543586520594 >500 0.0024009939798197394 0.47675727468569984 >1k 0.003201325306426319 1.8726695426024442 >5k 8.003313266065797E-4 1.381313143204748 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC 6556 0.4788858526337626 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC 6540 0.4777171257206845 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG 5638 0.41183014599590506 No Hit CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 3921 0.2864111391362085 TruSeq Adapter, Index 15 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 3720 0.27172900729066457 TruSeq Adapter, Index 15 (95% over 22bp) CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT 3454 0.2522989223607407 TruSeq Adapter, Index 14 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2946 0.21519184287051019 No Hit TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 2442 0.17837694510854915 TruSeq Adapter, Index 15 (95% over 22bp) GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT 1562 0.11409696488925217 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.460908641347659E-4 0.0 0.0 0.2682228265514302 0.0 2 1.460908641347659E-4 0.0 0.0 1.067778125961004 0.0 3 1.460908641347659E-4 0.0 0.0 1.503055855650539 0.0 4 1.460908641347659E-4 0.0 0.0 2.0793843146621906 0.0 5 1.460908641347659E-4 0.0 0.0 3.357898512137594 0.0 6 1.460908641347659E-4 0.0 0.0 4.097629602683981 0.0 7 1.460908641347659E-4 0.0 0.0 4.784987118438055 0.0 8 1.460908641347659E-4 0.0 0.0 5.771903951100466 0.0 9 1.460908641347659E-4 0.0 0.0 6.315946329138334 0.0 10 1.460908641347659E-4 0.0 0.0 7.6237517448727585 0.0 11 1.460908641347659E-4 0.0 0.0 8.974727010959006 0.0 12 1.460908641347659E-4 0.0 0.0 10.728693925761005 0.0 13 1.460908641347659E-4 0.0 0.0 11.193116782845426 0.0 14 1.460908641347659E-4 0.0 0.0 11.396913538313425 0.0 15 1.460908641347659E-4 0.0 0.0 11.733214707551657 0.0 16 2.1913629620214884E-4 0.0 0.0 12.254101683624164 0.0 17 2.921817282695318E-4 0.0 0.0 12.869436403359797 0.0 18 2.921817282695318E-4 0.0 0.0 13.509606569998342 0.0 19 2.921817282695318E-4 0.0 0.0 14.109309567271556 0.0 20 2.921817282695318E-4 0.0 0.0 14.686295435171814 7.304543206738295E-5 21 2.921817282695318E-4 0.0 0.0 15.459335242740927 7.304543206738295E-5 22 2.921817282695318E-4 0.0 0.0 16.350854741123335 7.304543206738295E-5 23 2.921817282695318E-4 0.0 0.0 17.12360236696418 7.304543206738295E-5 24 2.921817282695318E-4 0.0 0.0 17.702049143505786 7.304543206738295E-5 25 2.921817282695318E-4 0.0 0.0 18.237983478584177 7.304543206738295E-5 26 2.921817282695318E-4 0.0 0.0 18.71460492282385 7.304543206738295E-5 27 3.6522716033691473E-4 0.0 0.0 19.18465227817746 7.304543206738295E-5 28 3.6522716033691473E-4 0.0 0.0 19.665291221180837 7.304543206738295E-5 29 3.6522716033691473E-4 0.0 0.0 20.177047518244922 7.304543206738295E-5 30 3.6522716033691473E-4 0.0 0.0 20.74512184343296 7.304543206738295E-5 31 4.382725924042977E-4 0.0 0.0 21.305891625414258 7.304543206738295E-5 32 4.382725924042977E-4 0.0 0.0 21.84182596049265 7.304543206738295E-5 33 5.113180244716806E-4 0.0 0.0 22.33495567237955 7.304543206738295E-5 34 5.113180244716806E-4 0.0 0.0 22.83743519957108 7.304543206738295E-5 35 5.113180244716806E-4 0.0 0.0 23.356130812681563 7.304543206738295E-5 36 5.113180244716806E-4 0.0 0.0 23.913394413923626 7.304543206738295E-5 37 5.113180244716806E-4 0.0 0.0 24.480300012198587 7.304543206738295E-5 38 5.113180244716806E-4 0.0 0.0 25.011413348760527 7.304543206738295E-5 39 5.113180244716806E-4 0.0 0.0 25.542161458162134 7.304543206738295E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACGG 25 3.8911978E-5 45.0 2 GTTCACG 40 6.8175723E-9 45.0 1 ACCCGTA 20 7.03376E-4 45.0 11 TATTGCG 50 1.0822987E-9 40.5 1 TTGTTCG 325 0.0 40.153847 1 CGTTTTT 1690 0.0 39.27515 1 TTGGGAC 2755 0.0 38.87477 5 TTTGGGA 8000 0.0 38.75625 4 GGCGATA 70 0.0 38.57143 8 GGGCGAT 2355 0.0 38.21656 7 TCGTTCA 130 0.0 38.07692 16 TTGGGAT 4180 0.0 37.948563 5 TTCTTCG 405 0.0 37.777775 1 TTTTGCG 1150 0.0 37.565216 1 CGCATCG 30 1.1400361E-4 37.499996 21 CTTTGCG 480 0.0 37.499996 1 ATAACGC 30 1.1400361E-4 37.499996 11 TTGGGAG 5395 0.0 37.451344 5 TCGTTTG 455 0.0 37.08791 1 GGCGATT 1245 0.0 37.048195 8 >>END_MODULE