##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933919.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1369011
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45684877623335	33.0	31.0	34.0	31.0	34.0
2	32.68134733760357	34.0	31.0	34.0	31.0	34.0
3	32.735796133120914	34.0	31.0	34.0	31.0	34.0
4	36.10384284713563	37.0	35.0	37.0	35.0	37.0
5	36.1249705079068	37.0	35.0	37.0	35.0	37.0
6	35.67652706954144	37.0	35.0	37.0	35.0	37.0
7	36.02702827077357	37.0	35.0	37.0	35.0	37.0
8	36.001146813283455	37.0	36.0	37.0	35.0	37.0
9	37.846852216673206	39.0	38.0	39.0	35.0	39.0
10	37.61606517405631	39.0	37.0	39.0	35.0	39.0
11	37.60696882640096	39.0	37.0	39.0	35.0	39.0
12	37.461839240152194	39.0	37.0	39.0	35.0	39.0
13	37.400272897734204	39.0	37.0	39.0	35.0	39.0
14	38.68136779032455	40.0	38.0	41.0	35.0	41.0
15	38.72821620863529	40.0	38.0	41.0	35.0	41.0
16	38.81367571188252	40.0	38.0	41.0	35.0	41.0
17	38.74658567389159	40.0	38.0	41.0	35.0	41.0
18	38.692823505435676	40.0	38.0	41.0	35.0	41.0
19	38.694381564501676	40.0	38.0	41.0	35.0	41.0
20	38.61562105782934	40.0	38.0	41.0	35.0	41.0
21	38.567562276709246	40.0	38.0	41.0	34.0	41.0
22	38.548872872460485	40.0	38.0	41.0	34.0	41.0
23	38.477796014787316	40.0	38.0	41.0	34.0	41.0
24	38.445935058228166	40.0	38.0	41.0	34.0	41.0
25	38.37091082540608	40.0	38.0	41.0	34.0	41.0
26	38.28644766185224	40.0	37.0	41.0	34.0	41.0
27	38.254985533352176	40.0	37.0	41.0	34.0	41.0
28	38.1381990356542	40.0	37.0	41.0	34.0	41.0
29	38.1168792653967	40.0	37.0	41.0	34.0	41.0
30	38.059722675712614	40.0	37.0	41.0	34.0	41.0
31	37.98485987329539	40.0	37.0	41.0	34.0	41.0
32	37.86762414619021	40.0	37.0	41.0	33.0	41.0
33	37.77975049141315	40.0	37.0	41.0	33.0	41.0
34	37.70836392110801	40.0	37.0	41.0	33.0	41.0
35	37.64412411587635	40.0	36.0	41.0	33.0	41.0
36	37.51417994450008	40.0	36.0	41.0	33.0	41.0
37	37.436520232489	40.0	36.0	41.0	33.0	41.0
38	37.34282631768481	40.0	36.0	41.0	32.0	41.0
39	37.32463289191979	40.0	36.0	41.0	32.0	41.0
40	37.17043836755147	39.0	35.0	41.0	32.0	41.0
41	36.99790943973423	39.0	35.0	41.0	32.0	41.0
42	36.95096314054452	39.0	35.0	41.0	31.0	41.0
43	36.87353425209878	39.0	35.0	41.0	31.0	41.0
44	36.79362327987138	39.0	35.0	41.0	31.0	41.0
45	36.80433100975814	39.0	35.0	41.0	31.0	41.0
46	36.722122028237905	39.0	35.0	41.0	31.0	41.0
47	36.59446929206558	39.0	35.0	41.0	31.0	41.0
48	36.49589667285362	39.0	35.0	41.0	31.0	41.0
49	36.43485771845515	39.0	35.0	40.0	31.0	41.0
50	36.330935251798564	38.0	35.0	40.0	31.0	41.0
51	35.29393627954779	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	23.0
10	27.0
11	21.0
12	17.0
13	28.0
14	21.0
15	43.0
16	67.0
17	126.0
18	241.0
19	450.0
20	959.0
21	1454.0
22	2262.0
23	3079.0
24	4205.0
25	5900.0
26	7872.0
27	9581.0
28	11222.0
29	13160.0
30	16105.0
31	20801.0
32	27338.0
33	38121.0
34	69825.0
35	92453.0
36	98318.0
37	157153.0
38	295275.0
39	492535.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.670429236872454	4.631518665664483	52.893950450361615	16.804101647101447
2	19.82935126160418	7.0305497910535415	52.08665233515289	21.053446612189383
3	20.841687904626042	6.968315082932132	50.476146648931234	21.71385036351059
4	19.324242098858228	5.020047318830893	50.942687823545604	24.71302275876527
5	20.56594139857167	5.874532783155139	47.524161602792084	26.035364215481106
6	21.88645671948582	8.455081807231645	50.688197538222845	18.970263935059688
7	82.63030757240081	2.5198482700285094	10.218690719066538	4.631153438504146
8	83.32409308617682	5.050142036842655	7.0078326616805855	4.61793221529995
9	76.83488299217464	6.296735380504613	9.175748040008443	7.6926335873123
10	36.28232351675772	33.46276983895674	15.738880111262802	14.516026533022744
11	31.629475584929555	25.18555365880917	24.2406379495855	18.944332806675767
12	30.6856555571869	20.16294974985592	27.83352361668387	21.31787107627331
13	26.800880343547277	21.055053611694866	29.767401430667835	22.376664614090025
14	20.729636211834677	25.240702960020045	28.993412032481842	25.03624879566344
15	18.777423994401797	25.175911661776272	33.53588831645619	22.510776027365743
16	21.127003362281236	23.643053269842245	31.971474297869047	23.25846907000747
17	21.359580017983784	23.9513049931666	30.094133648305238	24.59498134054438
18	21.10596627784583	25.413528452291473	28.94987695496968	24.530628314893015
19	24.24348672143613	26.439232409381663	26.955152296073585	22.362128573108617
20	27.954413806755383	25.014042984314955	27.798169627563258	19.233373581366404
21	26.334412214364967	26.1166637813721	26.29803558919541	21.25088841506752
22	23.51697685409394	24.72244561950196	27.014757368640574	24.745820157763525
23	24.125664439511443	26.099352013972133	26.74164049814063	23.033343048375798
24	24.34239023645537	22.959274980259472	27.44630978129467	25.252025001990486
25	23.38805166649501	26.574877776730794	24.909076698434124	25.12799385834007
26	23.39418748278867	26.787513029478944	26.21490988750273	23.603389600229654
27	24.388481904089886	26.80358302453377	26.090075244099577	22.717859827276772
28	21.888282855287503	27.753758004866285	28.049445913875054	22.308513225971158
29	24.280228573766024	25.209950833119677	27.51614121435109	22.99367937876321
30	24.571533756850748	25.869039766663672	26.94507202644829	22.61435445003729
31	24.09469317631487	27.456682232648237	24.93179382780708	23.516830763229805
32	25.70271531784624	27.460699731411946	25.872692038267044	20.963892912474773
33	25.7428903054833	27.056758492079318	25.245670049400626	21.954681153036752
34	25.60059780381604	25.63551352034425	26.52747129131906	22.236417384520653
35	25.160864302770392	23.669641807114772	26.99766473753681	24.171829152578027
36	24.760502289609068	28.03695514499153	24.843554945869684	22.358987619529717
37	25.034860932454155	27.390064798602786	27.175238182892613	20.399836086050442
38	22.98827401679022	27.04295290541858	26.796132390462894	23.172640687328297
39	25.01228989394534	24.74684279381247	27.798242672995322	22.442624639246873
40	25.06780442231655	24.126467939264185	29.86688930914361	20.93883832927566
41	23.500687722742914	25.374376100703355	28.64345136744701	22.481484809106718
42	23.781401318177867	24.914920332999515	28.20912322837435	23.094555120448266
43	24.996146853458445	25.103888865757835	27.034187453570496	22.86577682721322
44	23.841152481608987	24.91141415226028	27.157342052036103	24.09009131409463
45	23.348826269474827	23.55269607037489	26.76165494652709	26.336822713623192
46	23.723987608572905	25.88087312665859	26.64164130164038	23.753497963128126
47	22.480023900465373	25.61476861763711	29.586833122597263	22.318374359300254
48	23.013547736285535	23.834432301858786	28.718176844451943	24.433843117403732
49	22.316329087202366	24.30075434017696	29.997202359951817	23.385714212668855
50	22.37819856816344	23.87935524258023	28.350100912264402	25.39234527699193
51	20.94417064581658	23.580818561720836	27.595760735304538	27.87925005715805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	846.0
2	1505.0
3	3663.5
4	5822.0
5	4055.5
6	2289.0
7	2269.5
8	2250.0
9	2230.5
10	2211.0
11	2219.5
12	2228.0
13	2251.5
14	2275.0
15	2255.5
16	2236.0
17	2217.0
18	2198.0
19	2436.5
20	2675.0
21	2961.0
22	3247.0
23	4111.0
24	4975.0
25	6273.0
26	9380.0
27	11189.0
28	13494.0
29	15799.0
30	17985.0
31	20171.0
32	24297.5
33	28424.0
34	32369.0
35	36314.0
36	38271.0
37	40228.0
38	44962.0
39	49696.0
40	54777.0
41	59858.0
42	63588.5
43	67319.0
44	74892.5
45	82466.0
46	103742.0
47	125018.0
48	125959.0
49	126900.0
50	126068.5
51	125237.0
52	110731.0
53	96225.0
54	88020.5
55	79816.0
56	75887.5
57	71959.0
58	72558.5
59	73158.0
60	72044.0
61	70930.0
62	61436.0
63	51942.0
64	43632.0
65	35322.0
66	28629.0
67	21936.0
68	17839.5
69	13743.0
70	11558.0
71	9373.0
72	7859.5
73	6346.0
74	5186.5
75	3052.0
76	2077.0
77	1544.0
78	1011.0
79	697.0
80	383.0
81	308.0
82	233.0
83	181.5
84	130.0
85	86.0
86	42.0
87	49.5
88	57.0
89	33.0
90	9.0
91	5.0
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1369011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.638410733034757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70797936066285	19.818945864059785
2	10.761189605165274	5.948443565672439
3	3.983049171668677	3.3025544692935824
4	1.8765270328765207	2.0745689954513717
5	1.2064140354869706	1.667168331344343
6	0.841826800676974	1.3960052929912075
7	0.6519833101168082	1.2613847761264345
8	0.520403467013282	1.1506499814566713
9	0.44488472085636416	1.1066315979491737
>10	7.620226172910238	49.78175553934106
>50	0.30948484630980166	5.28729726716909
>100	0.06962882564344564	3.4738543586520594
>500	0.0024009939798197394	0.47675727468569984
>1k	0.003201325306426319	1.8726695426024442
>5k	8.003313266065797E-4	1.381313143204748
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	6556	0.4788858526337626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	6540	0.4777171257206845	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	5638	0.41183014599590506	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3921	0.2864111391362085	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3720	0.27172900729066457	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	3454	0.2522989223607407	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2946	0.21519184287051019	No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2442	0.17837694510854915	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1562	0.11409696488925217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.460908641347659E-4	0.0	0.0	0.2682228265514302	0.0
2	1.460908641347659E-4	0.0	0.0	1.067778125961004	0.0
3	1.460908641347659E-4	0.0	0.0	1.503055855650539	0.0
4	1.460908641347659E-4	0.0	0.0	2.0793843146621906	0.0
5	1.460908641347659E-4	0.0	0.0	3.357898512137594	0.0
6	1.460908641347659E-4	0.0	0.0	4.097629602683981	0.0
7	1.460908641347659E-4	0.0	0.0	4.784987118438055	0.0
8	1.460908641347659E-4	0.0	0.0	5.771903951100466	0.0
9	1.460908641347659E-4	0.0	0.0	6.315946329138334	0.0
10	1.460908641347659E-4	0.0	0.0	7.6237517448727585	0.0
11	1.460908641347659E-4	0.0	0.0	8.974727010959006	0.0
12	1.460908641347659E-4	0.0	0.0	10.728693925761005	0.0
13	1.460908641347659E-4	0.0	0.0	11.193116782845426	0.0
14	1.460908641347659E-4	0.0	0.0	11.396913538313425	0.0
15	1.460908641347659E-4	0.0	0.0	11.733214707551657	0.0
16	2.1913629620214884E-4	0.0	0.0	12.254101683624164	0.0
17	2.921817282695318E-4	0.0	0.0	12.869436403359797	0.0
18	2.921817282695318E-4	0.0	0.0	13.509606569998342	0.0
19	2.921817282695318E-4	0.0	0.0	14.109309567271556	0.0
20	2.921817282695318E-4	0.0	0.0	14.686295435171814	7.304543206738295E-5
21	2.921817282695318E-4	0.0	0.0	15.459335242740927	7.304543206738295E-5
22	2.921817282695318E-4	0.0	0.0	16.350854741123335	7.304543206738295E-5
23	2.921817282695318E-4	0.0	0.0	17.12360236696418	7.304543206738295E-5
24	2.921817282695318E-4	0.0	0.0	17.702049143505786	7.304543206738295E-5
25	2.921817282695318E-4	0.0	0.0	18.237983478584177	7.304543206738295E-5
26	2.921817282695318E-4	0.0	0.0	18.71460492282385	7.304543206738295E-5
27	3.6522716033691473E-4	0.0	0.0	19.18465227817746	7.304543206738295E-5
28	3.6522716033691473E-4	0.0	0.0	19.665291221180837	7.304543206738295E-5
29	3.6522716033691473E-4	0.0	0.0	20.177047518244922	7.304543206738295E-5
30	3.6522716033691473E-4	0.0	0.0	20.74512184343296	7.304543206738295E-5
31	4.382725924042977E-4	0.0	0.0	21.305891625414258	7.304543206738295E-5
32	4.382725924042977E-4	0.0	0.0	21.84182596049265	7.304543206738295E-5
33	5.113180244716806E-4	0.0	0.0	22.33495567237955	7.304543206738295E-5
34	5.113180244716806E-4	0.0	0.0	22.83743519957108	7.304543206738295E-5
35	5.113180244716806E-4	0.0	0.0	23.356130812681563	7.304543206738295E-5
36	5.113180244716806E-4	0.0	0.0	23.913394413923626	7.304543206738295E-5
37	5.113180244716806E-4	0.0	0.0	24.480300012198587	7.304543206738295E-5
38	5.113180244716806E-4	0.0	0.0	25.011413348760527	7.304543206738295E-5
39	5.113180244716806E-4	0.0	0.0	25.542161458162134	7.304543206738295E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	25	3.8911978E-5	45.0	2
GTTCACG	40	6.8175723E-9	45.0	1
ACCCGTA	20	7.03376E-4	45.0	11
TATTGCG	50	1.0822987E-9	40.5	1
TTGTTCG	325	0.0	40.153847	1
CGTTTTT	1690	0.0	39.27515	1
TTGGGAC	2755	0.0	38.87477	5
TTTGGGA	8000	0.0	38.75625	4
GGCGATA	70	0.0	38.57143	8
GGGCGAT	2355	0.0	38.21656	7
TCGTTCA	130	0.0	38.07692	16
TTGGGAT	4180	0.0	37.948563	5
TTCTTCG	405	0.0	37.777775	1
TTTTGCG	1150	0.0	37.565216	1
CGCATCG	30	1.1400361E-4	37.499996	21
CTTTGCG	480	0.0	37.499996	1
ATAACGC	30	1.1400361E-4	37.499996	11
TTGGGAG	5395	0.0	37.451344	5
TCGTTTG	455	0.0	37.08791	1
GGCGATT	1245	0.0	37.048195	8
>>END_MODULE