##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933918.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	991273
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34787994830889	33.0	31.0	34.0	31.0	34.0
2	32.57749782350574	34.0	31.0	34.0	31.0	34.0
3	32.61474891377048	34.0	31.0	34.0	31.0	34.0
4	36.01866488848178	37.0	35.0	37.0	35.0	37.0
5	36.077758599296054	37.0	35.0	37.0	35.0	37.0
6	35.74640588415099	37.0	35.0	37.0	35.0	37.0
7	36.0847132929072	37.0	35.0	37.0	35.0	37.0
8	36.09917853104039	37.0	36.0	37.0	35.0	37.0
9	37.95348002013572	39.0	39.0	39.0	35.0	39.0
10	37.51835569010757	39.0	37.0	39.0	35.0	39.0
11	37.4376191019023	39.0	37.0	39.0	35.0	39.0
12	37.36198706108206	39.0	37.0	39.0	35.0	39.0
13	37.37492497021507	39.0	37.0	39.0	35.0	39.0
14	38.63276917660423	40.0	38.0	41.0	35.0	41.0
15	38.669926448112676	40.0	38.0	41.0	35.0	41.0
16	38.70196908419779	40.0	38.0	41.0	35.0	41.0
17	38.67859610823658	40.0	38.0	41.0	35.0	41.0
18	38.62288996068691	40.0	38.0	41.0	35.0	41.0
19	38.6109205032317	40.0	38.0	41.0	35.0	41.0
20	38.544629985886836	40.0	38.0	41.0	34.0	41.0
21	38.45789000608309	40.0	38.0	41.0	34.0	41.0
22	38.461952459110655	40.0	38.0	41.0	34.0	41.0
23	38.39127263629696	40.0	38.0	41.0	34.0	41.0
24	38.35820202910803	40.0	37.0	41.0	34.0	41.0
25	38.27421911017449	40.0	37.0	41.0	34.0	41.0
26	38.203508014442036	40.0	37.0	41.0	34.0	41.0
27	38.22932128687052	40.0	37.0	41.0	34.0	41.0
28	38.16813834332217	40.0	37.0	41.0	34.0	41.0
29	38.17203434371763	40.0	37.0	41.0	34.0	41.0
30	38.09528656586026	40.0	37.0	41.0	34.0	41.0
31	38.039219266539085	40.0	37.0	41.0	34.0	41.0
32	37.92745691651039	40.0	37.0	41.0	34.0	41.0
33	37.838607527895945	40.0	37.0	41.0	33.0	41.0
34	37.80057562346599	40.0	37.0	41.0	33.0	41.0
35	37.72238929134557	40.0	37.0	41.0	33.0	41.0
36	37.62930897946378	40.0	37.0	41.0	33.0	41.0
37	37.58446764917434	40.0	37.0	41.0	33.0	41.0
38	37.541532958125565	40.0	37.0	41.0	33.0	41.0
39	37.46408204399797	40.0	36.0	41.0	33.0	41.0
40	37.39661021736696	40.0	36.0	41.0	33.0	41.0
41	37.31569708849126	40.0	36.0	41.0	33.0	41.0
42	37.27730201468213	40.0	36.0	41.0	32.0	41.0
43	37.1925423167987	40.0	36.0	41.0	32.0	41.0
44	37.09753518959963	39.0	36.0	41.0	32.0	41.0
45	37.10622401699633	39.0	36.0	41.0	32.0	41.0
46	37.03124769866626	39.0	35.0	41.0	32.0	41.0
47	36.989667831162556	39.0	35.0	41.0	32.0	41.0
48	36.92355082807663	39.0	35.0	41.0	31.0	41.0
49	36.90964951128498	39.0	35.0	41.0	31.0	41.0
50	36.838875869715004	39.0	35.0	41.0	31.0	41.0
51	35.926558072296935	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	9.0
10	13.0
11	11.0
12	9.0
13	9.0
14	4.0
15	13.0
16	22.0
17	61.0
18	103.0
19	213.0
20	388.0
21	616.0
22	1106.0
23	1637.0
24	2673.0
25	4472.0
26	6536.0
27	7616.0
28	7983.0
29	9317.0
30	11272.0
31	14097.0
32	18823.0
33	26569.0
34	46221.0
35	64328.0
36	70091.0
37	110766.0
38	223046.0
39	363040.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.022921031844913	4.799686867290847	58.71752786568383	13.459864235180419
2	20.637402612600162	4.103208702345368	54.00399284556323	21.25539583949124
3	19.765392581054865	4.310316128856531	53.71123797379733	22.213053316291273
4	17.82990155083413	4.613360799698973	53.55315841347439	24.003579235992508
5	17.746372593624564	5.53671894624387	53.425342968082454	23.291565492049113
6	20.561137042974035	6.125557742418083	55.20759669636921	18.105708518238668
7	83.37370229997185	1.7392786850847344	10.175098080952472	4.711920933990939
8	85.80905562846965	2.0056028964775594	7.621613823840658	4.563727651212128
9	80.32519800297193	4.936379786395877	9.818990328597673	4.919431882034515
10	35.569918680323184	31.559116408900472	18.741860214088348	14.129104696687996
11	25.312300445992175	26.255330267242222	28.430210446567195	20.002158840198412
12	22.2862924744243	22.84002489727855	32.95731851871281	21.916364109584343
13	22.619903901347055	24.419912577060003	33.46827765913124	19.491905862461703
14	20.912200776173666	26.23475066908914	31.54569931794773	21.30734923678946
15	18.395336098128368	26.170590745435412	34.17252361357567	21.261549542860543
16	19.965741021898104	26.60256054588393	31.814343778151933	21.617354654066034
17	20.415062248240393	26.4599156841758	29.461107081500256	23.66391498608355
18	20.964557695004302	26.129532429512352	31.49011422685779	21.415795648625554
19	21.70895404192387	28.37069102053622	28.87307532839087	21.047279609149044
20	22.956945261295324	27.928834942543578	29.439922201048553	19.67429759511255
21	22.735815461532795	28.257402350311168	29.521837072128466	19.48494511602757
22	22.15202068451375	25.53131175770953	29.07291936731859	23.243748190458128
23	20.474884315420677	27.652927094755935	28.144517201618523	23.72767138820486
24	21.44545448125794	26.398176889716556	29.24290281284772	22.913465816177784
25	20.118070400384152	27.959099057474578	27.34857097893315	24.574259563208116
26	19.145886148417237	28.817591117684028	28.040206885489667	23.99631584840907
27	19.8056438539131	27.63335630043389	28.50486193006366	24.05613791558935
28	18.278920136027107	26.7193800295176	30.708694779339297	24.293005055115994
29	19.333523660989453	24.256082834900177	30.561510300391515	25.848883203718852
30	20.412136717130398	25.33963902981318	30.46093255843748	23.78729169461894
31	20.44572988470381	26.360548506819008	29.33793213373107	23.855789474746107
32	20.238521577809543	27.02837664296314	27.853880817897792	24.879220961329523
33	20.55800975109783	25.34226191977387	28.905558811750144	25.194169517378157
34	19.23556880899611	24.334971294486987	30.77729343984957	25.652166456667334
35	18.560981687184057	24.420820500507933	28.870048916897765	28.148148895410248
36	18.928388042446432	24.667372156812505	29.8428384511633	26.561401349577768
37	18.452535275347962	25.48974903987095	32.05766726219719	24.000048422583887
38	19.729075643137662	25.679605920871445	30.914188119720805	23.67713031627009
39	20.331634171413928	24.48750243373924	32.637426823892106	22.54343657095472
40	20.571931243966095	23.679147923932156	32.0578690229634	23.69105180913835
41	18.33188233715636	24.47297565857236	30.18583175371467	27.009310250556606
42	19.071335545303867	24.625506797824613	29.218792401286027	27.084365255585496
43	19.498866608895835	24.362108117541787	30.603577420145612	25.53544785341677
44	19.92780999785125	24.38783261523314	29.3539721146445	26.330385272271112
45	20.622270555134662	23.574333205887783	28.346378848208314	27.457017390769245
46	20.62741545467293	23.953139044440835	29.24582834395772	26.173617156928515
47	18.23372572439681	24.18082606910508	31.34403943212415	26.24140877437396
48	18.511045897547902	23.469619368226514	31.37692643701584	26.64240829720975
49	20.481845061854806	22.062438904317982	32.660629312005874	24.795086721821335
50	20.356148104508044	21.611907113378454	31.558914648134266	26.473030133979236
51	18.201141360654432	21.583660606109518	30.138518854039198	30.07667917919685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	256.5
2	452.0
3	2788.5
4	5125.0
5	3506.5
6	1888.0
7	1921.0
8	1954.0
9	2246.0
10	2538.0
11	2738.0
12	2938.0
13	3006.0
14	3074.0
15	3045.5
16	3017.0
17	2884.5
18	2752.0
19	2909.5
20	3067.0
21	3127.5
22	3188.0
23	3571.5
24	3955.0
25	4675.0
26	6232.0
27	7069.0
28	8329.5
29	9590.0
30	11808.0
31	14026.0
32	16181.5
33	18337.0
34	21627.0
35	24917.0
36	28340.0
37	31763.0
38	36422.5
39	41082.0
40	45551.0
41	50020.0
42	57496.5
43	64973.0
44	72111.5
45	79250.0
46	94141.0
47	109032.0
48	120812.0
49	132592.0
50	125543.5
51	118495.0
52	98846.0
53	79197.0
54	66031.5
55	52866.0
56	45078.0
57	37290.0
58	32803.0
59	28316.0
60	24934.0
61	21552.0
62	17190.0
63	12828.0
64	10556.0
65	8284.0
66	6508.5
67	4733.0
68	3666.0
69	2599.0
70	1954.5
71	1310.0
72	1055.5
73	801.0
74	631.5
75	402.0
76	342.0
77	219.0
78	96.0
79	64.0
80	32.0
81	20.0
82	8.0
83	5.0
84	2.0
85	3.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	991273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.69607645712363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.30624393619436	27.256616997462146
2	11.903335211098522	8.974180684246836
3	4.444640581323242	5.02636533533986
4	2.3362976958206643	3.5227702627303015
5	1.4138989122559242	2.664922074952163
6	0.9848686923302673	2.2275411315785476
7	0.7085523138778159	1.8696749538467006
8	0.5977607178583646	1.8026586978763217
9	0.5050223212872169	1.7133624032217287
>10	4.582362720565602	34.77250634698305
>50	0.1649228262021477	4.008440112809491
>100	0.046155886908134554	2.9825477661885023
>500	0.003778844540310395	0.9612161409157117
>1k	0.002159339737320226	2.2171970918486585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	3796	0.38294193426028955	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3591	0.36226145572410423	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3542	0.3573183169520404	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3283	0.3311902977282747	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3189	0.32170754171656046	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1902	0.19187448866255816	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1478	0.14910120622674078	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	1010	0.1018891869343763	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40079776206958123	0.0
2	0.0	0.0	0.0	1.5298510097621947	0.0
3	0.0	0.0	0.0	2.3374993568875575	0.0
4	0.0	0.0	0.0	3.17621886200875	0.0
5	0.0	0.0	0.0	4.543652454974563	0.0
6	0.0	0.0	0.0	5.724558219582295	0.0
7	0.0	0.0	0.0	6.691698452394043	0.0
8	0.0	0.0	0.0	8.270173806812048	0.0
9	0.0	0.0	0.0	9.104151933927385	0.0
10	1.0088038310334287E-4	0.0	0.0	10.405912397492921	0.0
11	1.0088038310334287E-4	0.0	0.0	12.662404806748494	0.0
12	1.0088038310334287E-4	0.0	0.0	14.797134593598333	0.0
13	1.0088038310334287E-4	0.0	0.0	15.602664452678525	0.0
14	1.0088038310334287E-4	0.0	0.0	15.911459305357859	0.0
15	1.0088038310334287E-4	0.0	0.0	16.240430234657858	0.0
16	2.0176076620668574E-4	0.0	0.0	16.939733050330233	0.0
17	2.0176076620668574E-4	0.0	0.0	18.025306852905306	0.0
18	2.0176076620668574E-4	0.0	0.0	19.19511577537167	0.0
19	2.0176076620668574E-4	0.0	0.0	20.063494113125245	0.0
20	2.0176076620668574E-4	0.0	0.0	20.93943847961157	0.0
21	2.0176076620668574E-4	0.0	0.0	22.198324780358185	0.0
22	2.0176076620668574E-4	0.0	0.0	23.6140800768305	0.0
23	2.0176076620668574E-4	0.0	0.0	25.00854961246801	0.0
24	2.0176076620668574E-4	0.0	0.0	25.989106936232503	0.0
25	2.0176076620668574E-4	0.0	0.0	26.842857618436092	0.0
26	2.0176076620668574E-4	0.0	0.0	27.62922020472665	0.0
27	2.0176076620668574E-4	0.0	0.0	28.37502887700966	0.0
28	2.0176076620668574E-4	0.0	0.0	29.213344860598443	0.0
29	2.0176076620668574E-4	0.0	0.0	30.048735313077223	0.0
30	2.0176076620668574E-4	0.0	0.0	30.994690665437272	0.0
31	2.0176076620668574E-4	0.0	0.0	31.864884850086707	0.0
32	2.0176076620668574E-4	0.0	0.0	32.71117038394065	0.0
33	2.0176076620668574E-4	0.0	0.0	33.519524893747736	0.0
34	2.0176076620668574E-4	0.0	0.0	34.29418535559831	0.0
35	2.0176076620668574E-4	0.0	0.0	35.15055892776258	0.0
36	2.0176076620668574E-4	0.0	0.0	35.938434719799695	0.0
37	2.0176076620668574E-4	0.0	0.0	36.746688349223675	0.0
38	3.026411493100286E-4	0.0	0.0	37.531033327852164	0.0
39	3.026411493100286E-4	0.0	0.0	38.35552869895579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGT	25	3.8904152E-5	45.000004	12
CCCGCGG	25	3.8904152E-5	45.000004	2
TCGTTAG	35	1.2117016E-7	45.0	1
GCAACGC	20	7.032816E-4	45.0	37
CCGGATA	20	7.032816E-4	45.0	20
GACGGCG	45	3.8380676E-10	45.0	9
CGATCGT	20	7.032816E-4	45.0	10
GTACGAA	20	7.032816E-4	45.0	9
CGCACGG	30	2.1651995E-6	44.999996	2
CGTTTTT	2240	0.0	43.292408	1
ATGGGCG	90	0.0	40.0	5
GTTTACG	45	1.927765E-8	40.0	1
TATTGCG	90	0.0	40.0	1
TACGGGT	40	3.4575714E-7	39.375	4
TTACACG	180	0.0	38.75	34
TTCGCAG	35	6.247423E-6	38.571426	1
AATCGTG	35	6.247423E-6	38.571426	10
GCGAGGC	35	6.247423E-6	38.571426	9
TTTGTCG	345	0.0	38.47826	1
TTTCGCG	305	0.0	38.360657	1
>>END_MODULE