##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933917.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627134
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43353573558442	33.0	31.0	34.0	31.0	34.0
2	32.64633395733607	34.0	31.0	34.0	31.0	34.0
3	32.71853862172996	34.0	31.0	34.0	31.0	34.0
4	36.08836707944395	37.0	35.0	37.0	35.0	37.0
5	36.11068128980409	37.0	35.0	37.0	35.0	37.0
6	35.64336170579175	37.0	35.0	37.0	35.0	37.0
7	36.00250823587941	37.0	35.0	37.0	35.0	37.0
8	35.990437450369456	37.0	35.0	37.0	35.0	37.0
9	37.848815404682256	39.0	38.0	39.0	35.0	39.0
10	37.59137441121036	39.0	37.0	39.0	35.0	39.0
11	37.538368195632835	39.0	37.0	39.0	35.0	39.0
12	37.35031109778772	39.0	37.0	39.0	35.0	39.0
13	37.27598886362404	39.0	37.0	39.0	34.0	39.0
14	38.522663418025495	40.0	38.0	41.0	34.0	41.0
15	38.58224079702265	40.0	38.0	41.0	35.0	41.0
16	38.68376136519468	40.0	38.0	41.0	35.0	41.0
17	38.622450704315185	40.0	38.0	41.0	35.0	41.0
18	38.58956140155055	40.0	38.0	41.0	35.0	41.0
19	38.606830438151974	40.0	38.0	41.0	35.0	41.0
20	38.5318321124353	40.0	38.0	41.0	34.0	41.0
21	38.4521489825141	40.0	38.0	41.0	34.0	41.0
22	38.44136819244372	40.0	38.0	41.0	34.0	41.0
23	38.36223837329821	40.0	37.0	41.0	34.0	41.0
24	38.33202473474569	40.0	37.0	41.0	34.0	41.0
25	38.25964945290799	40.0	37.0	41.0	34.0	41.0
26	38.1903293395032	40.0	37.0	41.0	34.0	41.0
27	38.165937423262015	40.0	37.0	41.0	34.0	41.0
28	38.10091463706321	40.0	37.0	41.0	34.0	41.0
29	38.08676136200557	40.0	37.0	41.0	34.0	41.0
30	38.036690723194724	40.0	37.0	41.0	34.0	41.0
31	37.98912672570774	40.0	37.0	41.0	34.0	41.0
32	37.874787525473025	40.0	36.0	41.0	34.0	41.0
33	37.81586869791783	40.0	36.0	41.0	33.0	41.0
34	37.77695516428706	40.0	36.0	41.0	33.0	41.0
35	37.742055126974456	40.0	36.0	41.0	33.0	41.0
36	37.665718969151726	40.0	36.0	41.0	33.0	41.0
37	37.5871615954485	40.0	36.0	41.0	33.0	41.0
38	37.56812100763154	40.0	36.0	41.0	33.0	41.0
39	37.48998938026004	40.0	36.0	41.0	33.0	41.0
40	37.39492038384141	40.0	35.0	41.0	33.0	41.0
41	37.25861618091189	40.0	35.0	41.0	33.0	41.0
42	37.25991255457367	40.0	35.0	41.0	33.0	41.0
43	37.15051647654249	39.0	35.0	41.0	32.0	41.0
44	37.08174967391339	39.0	35.0	41.0	32.0	41.0
45	37.09865515184953	39.0	35.0	41.0	32.0	41.0
46	37.034351510203564	39.0	35.0	41.0	32.0	41.0
47	36.92722767383047	39.0	35.0	41.0	32.0	41.0
48	36.885354645099774	39.0	35.0	41.0	32.0	41.0
49	36.8793096850115	39.0	35.0	41.0	32.0	41.0
50	36.79886595209317	39.0	35.0	41.0	32.0	41.0
51	35.89567779772744	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	12.0
11	17.0
12	11.0
13	13.0
14	10.0
15	13.0
16	30.0
17	41.0
18	82.0
19	177.0
20	335.0
21	601.0
22	843.0
23	1289.0
24	1683.0
25	2434.0
26	3250.0
27	4013.0
28	4632.0
29	5587.0
30	6796.0
31	8905.0
32	12227.0
33	17741.0
34	33906.0
35	47970.0
36	42197.0
37	66873.0
38	131846.0
39	233426.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.14954060854618	4.814122659591092	52.15711474740645	15.879221984456274
2	19.712214614420525	7.340217561159178	51.10438917360563	21.84317865081466
3	20.686328599629427	7.389010960974847	49.52689536845395	22.397765070941777
4	19.175806127558065	4.8216170706738914	50.92755296316258	25.075023838605464
5	20.12536395730418	6.217172087624016	47.698896886470834	25.958567068600967
6	21.900263739487894	9.051016210251717	50.76522720822025	18.28349284204014
7	82.51490112161038	2.8274659004295732	10.212171561420686	4.445461416539368
8	83.09420315275523	5.343993468700469	6.864083274069019	4.697720104475279
9	76.93858090934313	6.076532288155322	9.141108598800257	7.843778203701282
10	38.15006043365533	31.290601370679948	15.772227307082698	14.787110888582026
11	31.1824905044217	24.83217302841179	24.940602805779946	19.044733661386562
12	28.993165734914705	21.84844068412811	28.258394537690513	20.89999904326667
13	25.3687409708292	23.652520832868255	30.14395647501172	20.834781721290824
14	20.700998510685118	27.038559542298774	29.179250367545055	23.081191579471056
15	18.014491320834143	25.30113181552906	34.54827835837317	22.136098505263625
16	20.853119110110438	24.18526184196679	32.1436885896794	22.817930458243374
17	20.427053867275575	24.059610864663693	30.050515519809164	25.46281974825157
18	20.855351487879783	24.65948266239751	30.43831142945527	24.046854420267437
19	23.029846890776135	26.513631855392944	27.856247628098618	22.6002736257323
20	26.831586231969563	25.83387282462759	28.919337812971392	18.415203130431454
21	25.46234138158671	27.01719249793505	27.11286583090695	20.40760028957129
22	23.07848083503685	24.07858607570312	28.116638549337143	24.72629453992289
23	22.540637248179817	26.919446242748755	27.758341917357377	22.78157459171405
24	22.630251270063496	23.382243667222635	29.586659310450397	24.40084575226347
25	21.707163062439605	26.952612998179013	26.778009165505303	24.56221477387608
26	21.344401674921148	27.353165352221374	27.336103607841387	23.96632936501609
27	21.612127551687518	27.10090666428546	27.946021105537255	23.340944678489763
28	18.664910529488115	27.112706375351998	30.48885884037542	23.733524254784463
29	20.389900722971486	24.755315450924364	29.098725312293706	25.756058513810448
30	21.270414297422878	25.600110981066248	29.233943622893992	23.895531098616882
31	22.387241004314866	27.058810397777826	25.290448293347197	25.26350030456011
32	22.756380614031453	28.87771991312861	24.649755873545367	23.71614359929457
33	22.36316321551694	26.785663032143052	25.191585849276233	25.65958790306378
34	21.527775563117295	26.578530266258888	26.592721810649717	25.300972359974104
35	21.052757464911807	25.573162992279165	26.44442814454327	26.929651398265765
36	20.68106656631597	27.307720519059725	25.834510646847402	26.176702267776903
37	20.762707810451992	28.023835416354398	28.281834504268627	22.931622268924983
38	20.837333010170074	27.897227705721583	24.97265337232553	26.292785911782808
39	20.98801850960082	28.010600605293284	26.140665312357488	24.860715572748408
40	22.207375138327695	25.172929549346712	28.171491260241034	24.448204052084563
41	19.724333236596962	27.491253862810815	27.456811462940934	25.327601437651282
42	21.973613294766352	25.84184560237525	26.72459155459599	25.45994954826241
43	21.370392930378515	26.460214244483637	26.7228375434915	25.44655528164635
44	20.79635293254711	25.707105658439822	27.01304665350627	26.483494755506797
45	20.567055844524457	24.20152630857201	26.03032206832989	29.201095778573638
46	21.76424815111284	25.828610791314137	26.888511865087843	25.518629192485175
47	19.183619449750772	25.632002092056883	30.18685001929412	24.997528438898225
48	19.99748060223174	24.55216907391403	28.869587679826004	26.580762644028226
49	20.28386277892763	24.169954108691286	29.800489209642596	25.74569390273849
50	20.431199711704355	24.423647896621777	27.693443506491434	27.451708885182434
51	19.819687658458957	24.44357984099092	25.975309901871054	29.761422598679072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	350.0
2	588.0
3	1104.5
4	1621.0
5	1206.5
6	792.0
7	765.5
8	739.0
9	833.5
10	928.0
11	938.0
12	948.0
13	916.5
14	885.0
15	845.5
16	806.0
17	910.5
18	1015.0
19	1093.5
20	1172.0
21	1418.0
22	1664.0
23	1925.5
24	2187.0
25	2636.0
26	4098.5
27	5112.0
28	6237.0
29	7362.0
30	8624.0
31	9886.0
32	11304.0
33	12722.0
34	14474.5
35	16227.0
36	17889.5
37	19552.0
38	21587.5
39	23623.0
40	27252.0
41	30881.0
42	33178.5
43	35476.0
44	38955.5
45	42435.0
46	52233.0
47	62031.0
48	63274.0
49	64517.0
50	63785.0
51	63053.0
52	54456.5
53	45860.0
54	40861.0
55	35862.0
56	33749.5
57	31637.0
58	29917.5
59	28198.0
60	26973.0
61	25748.0
62	22187.0
63	18626.0
64	15361.5
65	12097.0
66	9929.5
67	7762.0
68	6065.5
69	4369.0
70	3579.0
71	2789.0
72	2454.0
73	2119.0
74	1584.0
75	932.5
76	816.0
77	634.5
78	453.0
79	287.0
80	121.0
81	85.0
82	49.0
83	57.5
84	66.0
85	47.5
86	29.0
87	25.5
88	22.0
89	27.5
90	33.0
91	21.0
92	9.0
93	4.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	627134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.583202697402022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.47615648415088	19.43958109636157
2	11.78284862559424	6.500174039851414
3	4.532617959401525	3.7507235977217093
4	2.257000042657219	2.490211586586364
5	1.458093830012076	2.010944883252717
6	0.9140460298845762	1.5127390150237112
7	0.6724826368560433	1.2984457418018545
8	0.5051358727115046	1.1146612133384397
9	0.39977374598207194	0.9924336241670878
>10	6.296741675094057	44.6818844033746
>50	0.6448328743707246	10.623351374292483
>100	0.05266330189960537	2.475107390339796
>500	0.0035108867933070255	0.618494393203919
>1k	0.0040960345921915295	2.491247640684366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	3354	0.5348139313129252	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	2934	0.4678425982325946	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	2326	0.3708936208210686	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1967	0.31364907659288127	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1753	0.2795255878329033	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1738	0.27713375450860583	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1363	0.21733792140116787	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	793	0.12644825507786214	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	784	0.12501315508328364	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1891110990633583E-4	0.0	0.0	0.28925237668504655	0.0
2	3.1891110990633583E-4	0.0	0.0	1.158444606734765	0.0
3	3.1891110990633583E-4	0.0	0.0	1.6227791827583897	0.0
4	3.1891110990633583E-4	0.0	0.0	2.3063651468426207	0.0
5	3.1891110990633583E-4	0.0	0.0	3.6338326418277433	0.0
6	3.1891110990633583E-4	0.0	0.0	4.232747706231842	0.0
7	3.1891110990633583E-4	0.0	0.0	4.840432826158365	0.0
8	3.1891110990633583E-4	0.0	0.0	5.824433055774364	0.0
9	3.1891110990633583E-4	0.0	0.0	6.270589698533327	0.0
10	3.1891110990633583E-4	0.0	0.0	7.5337966048723235	0.0
11	3.1891110990633583E-4	0.0	0.0	9.152270487646978	0.0
12	3.1891110990633583E-4	0.0	0.0	11.344624912698084	0.0
13	3.1891110990633583E-4	0.0	0.0	11.862696010740926	0.0
14	3.1891110990633583E-4	0.0	0.0	12.037299843414646	0.0
15	3.1891110990633583E-4	0.0	0.0	12.397509942053851	0.0
16	3.1891110990633583E-4	0.0	0.0	13.025286461904473	0.0
17	3.1891110990633583E-4	0.0	0.0	13.819215670016296	0.0
18	3.1891110990633583E-4	0.0	0.0	14.678362200103965	0.0
19	3.1891110990633583E-4	0.0	0.0	15.400058041822003	0.0
20	3.1891110990633583E-4	0.0	0.0	16.106286694709585	0.0
21	3.1891110990633583E-4	0.0	0.0	17.07657374659961	0.0
22	3.1891110990633583E-4	0.0	0.0	18.09884330940437	0.0
23	3.1891110990633583E-4	0.0	0.0	19.06275213909627	0.0
24	3.1891110990633583E-4	0.0	0.0	19.774083369742353	0.0
25	3.1891110990633583E-4	0.0	0.0	20.413659600659507	0.0
26	3.1891110990633583E-4	0.0	0.0	20.956605765275047	0.0
27	3.1891110990633583E-4	0.0	0.0	21.478982163301623	0.0
28	3.1891110990633583E-4	0.0	0.0	22.039468438962007	0.0
29	3.1891110990633583E-4	0.0	0.0	22.612232792353787	0.0
30	3.1891110990633583E-4	0.0	0.0	23.27875701205803	0.0
31	3.1891110990633583E-4	0.0	0.0	23.89999585415557	0.0
32	3.1891110990633583E-4	0.0	0.0	24.51804558515405	0.0
33	3.1891110990633583E-4	0.0	0.0	25.084591171902655	0.0
34	3.1891110990633583E-4	0.0	0.0	25.667401225256484	0.0
35	3.1891110990633583E-4	0.0	0.0	26.250370734165266	0.0
36	3.1891110990633583E-4	0.0	0.0	26.854866742992726	0.0
37	3.1891110990633583E-4	0.0	0.0	27.412004451999096	0.0
38	3.1891110990633583E-4	0.0	0.0	27.97153399432976	0.0
39	3.1891110990633583E-4	0.0	0.0	28.5648681143105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAT	30	2.163917E-6	45.000004	28
TTTAGCG	25	3.8887716E-5	45.0	1
CGAACTA	20	7.030833E-4	45.0	18
CGTATCG	20	7.030833E-4	45.0	33
CTATCGA	25	3.8887716E-5	45.0	34
TTGTCGT	20	7.030833E-4	45.0	29
TACGGTG	20	7.030833E-4	45.0	1
CGTTCGG	25	3.8887716E-5	45.0	2
CCCCGAT	25	3.8887716E-5	45.0	40
CGTAATA	20	7.030833E-4	45.0	31
CCAACGT	20	7.030833E-4	45.0	19
CGGTAGA	25	3.8887716E-5	45.0	24
CCTATCG	25	3.8887716E-5	45.0	33
TCGACAT	25	3.8887716E-5	45.0	37
TATCGGT	20	7.030833E-4	45.0	35
TGTAACG	20	7.030833E-4	45.0	13
CGATATA	25	3.8887716E-5	45.0	20
AGTACGA	20	7.030833E-4	45.0	13
GTAACGA	20	7.030833E-4	45.0	14
CGCCAAT	20	7.030833E-4	45.0	16
>>END_MODULE