##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933915.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1870008
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31912804651103	33.0	31.0	34.0	31.0	34.0
2	32.52933730764788	34.0	31.0	34.0	31.0	34.0
3	32.58107826276679	34.0	31.0	34.0	31.0	34.0
4	36.02617635860381	37.0	35.0	37.0	35.0	37.0
5	36.03459022635197	37.0	35.0	37.0	35.0	37.0
6	35.49188987426792	37.0	35.0	37.0	33.0	37.0
7	35.90253785010545	37.0	35.0	37.0	35.0	37.0
8	35.83639481756228	37.0	35.0	37.0	35.0	37.0
9	37.746289320687396	39.0	38.0	39.0	35.0	39.0
10	37.52229027897207	39.0	37.0	39.0	35.0	39.0
11	37.46560977279242	39.0	37.0	39.0	35.0	39.0
12	37.19161629255062	39.0	37.0	39.0	34.0	39.0
13	37.03737577593251	39.0	37.0	39.0	33.0	39.0
14	38.01409352259456	40.0	37.0	41.0	33.0	41.0
15	38.10004449178827	40.0	37.0	41.0	33.0	41.0
16	38.30748210702842	40.0	37.0	41.0	33.0	41.0
17	38.22481240721965	40.0	37.0	41.0	33.0	41.0
18	38.186734495253496	40.0	37.0	41.0	34.0	41.0
19	38.1684987443904	40.0	37.0	41.0	34.0	41.0
20	37.97098889416516	40.0	36.0	41.0	33.0	41.0
21	37.985400062459625	40.0	36.0	41.0	33.0	41.0
22	38.031534089693736	40.0	36.0	41.0	34.0	41.0
23	37.935355891525596	40.0	36.0	41.0	34.0	41.0
24	37.911149043212646	40.0	36.0	41.0	34.0	41.0
25	37.84407820715205	40.0	35.0	41.0	34.0	41.0
26	37.78116938537161	40.0	35.0	41.0	34.0	41.0
27	37.731970665366134	40.0	35.0	41.0	33.0	41.0
28	37.66275759248089	40.0	35.0	41.0	33.0	41.0
29	37.62018772112205	40.0	35.0	41.0	33.0	41.0
30	37.5145849643424	40.0	35.0	41.0	33.0	41.0
31	37.41392603668006	40.0	35.0	41.0	33.0	41.0
32	37.21974237543369	40.0	35.0	41.0	32.0	41.0
33	37.04627359882952	39.0	35.0	41.0	32.0	41.0
34	36.86212732779753	39.0	35.0	41.0	31.0	41.0
35	36.76287588074489	39.0	35.0	41.0	31.0	41.0
36	36.62248022468353	39.0	35.0	41.0	31.0	41.0
37	36.51595661622838	39.0	35.0	41.0	31.0	41.0
38	36.433722743432114	39.0	35.0	41.0	30.0	41.0
39	36.24712193744626	39.0	35.0	41.0	30.0	41.0
40	35.984687231284575	39.0	35.0	41.0	29.0	41.0
41	35.78972710277175	38.0	35.0	40.0	28.0	41.0
42	35.76064220046118	38.0	35.0	40.0	29.0	41.0
43	35.598715620467935	37.0	35.0	40.0	29.0	41.0
44	35.52894800450051	37.0	35.0	40.0	29.0	41.0
45	35.48444231254626	37.0	34.0	40.0	29.0	41.0
46	35.37248182895474	37.0	34.0	40.0	29.0	41.0
47	35.139158762957166	36.0	34.0	40.0	28.0	41.0
48	35.082374513905826	36.0	34.0	40.0	28.0	41.0
49	35.05938156414304	36.0	34.0	40.0	29.0	41.0
50	34.91249235297389	35.0	34.0	40.0	28.0	41.0
51	34.01302775175294	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	22.0
10	42.0
11	36.0
12	31.0
13	36.0
14	38.0
15	74.0
16	142.0
17	251.0
18	467.0
19	1003.0
20	2015.0
21	3589.0
22	5423.0
23	7050.0
24	9551.0
25	13086.0
26	16894.0
27	19134.0
28	21265.0
29	24189.0
30	30075.0
31	37692.0
32	50091.0
33	72805.0
34	138135.0
35	146091.0
36	151572.0
37	242857.0
38	354503.0
39	521487.0
40	354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.5309389050742	4.190089026357106	48.77246514453414	16.50650692403455
2	20.951835500168983	11.258721887820801	46.326165449559575	21.46327716245064
3	19.749862032675797	11.542677892287092	47.59241671693383	21.11504335810328
4	18.05794413713738	4.8229740193624835	54.267575325880955	22.85150651761918
5	22.839314056410455	5.8779962438663365	46.85947867602705	24.423211023696155
6	20.597131135267873	11.046316379395169	50.89668065591163	17.459871829425328
7	77.627849720429	2.4188666572549424	15.271431993873824	4.681851628442231
8	77.67838426359674	7.257027777421272	10.221186219524196	4.843401739457799
9	72.39594696921083	5.00783953865438	11.48187601336465	11.114337478770144
10	41.14126784484344	24.77572288460798	19.24526526089728	14.837744009651296
11	33.07964457906063	21.36958772368888	26.585126908547984	18.96564078870251
12	28.37699090057369	19.047886426154324	30.456447245145473	22.11867542812651
13	27.009189265500467	19.417403561909897	34.400013261975346	19.173393910614287
14	20.948252627796247	23.305729173351132	33.6802302450043	22.065787953848325
15	18.821844612429466	21.5256298368777	38.034543167729765	21.617982382963067
16	23.926100850905453	22.790544211575565	29.584846695843016	23.698508241675974
17	23.716262176418496	24.259789262933634	30.45703547792309	21.566913082724778
18	23.958239750845987	22.790009454505007	30.838852026301495	22.412898768347514
19	23.395300982669593	25.1356143930935	28.583727984051404	22.8853566401855
20	24.014977476032186	27.812929142549127	30.210405516981748	17.961687864436943
21	23.5064769776386	27.677582127990895	31.049546312101338	17.766394582269164
22	22.06979863187751	21.689586354710784	33.67113937480482	22.569475638606892
23	22.242578641374795	24.74684600279785	32.166600356789914	20.84397499903744
24	23.365408062425402	24.629680728638593	29.01196144615424	22.992949762781763
25	22.697870811247867	28.001003204264368	26.348229526290794	22.95289645819697
26	21.478945544617993	23.458937074065993	29.05319121629426	26.008926165021755
27	23.276584913005717	23.259205308212586	29.64522076910901	23.818989009672688
28	19.373713909245307	24.41936077278814	29.91554046827607	26.29138484969048
29	24.93395750178609	23.34455253667364	27.823998613909673	23.8974913476306
30	26.128176991756185	24.736043909972576	28.424370377025127	20.711408721246112
31	25.44197671881618	23.420594992106984	26.871596271245902	24.26583201783094
32	29.36506153984368	25.01390368383451	24.627381273235194	20.99365350308662
33	26.304325970797986	25.305934520066227	24.67326342988907	23.71647607924672
34	23.70144940556404	26.100637002622452	28.15201860098994	22.04589499082357
35	23.972036483266383	28.827844586761124	25.72218942378856	21.477929506183933
36	24.932246279160303	30.193988474915617	23.67091477683518	21.2028504690889
37	24.26171438838764	28.447953163836733	26.114754589285177	21.175577858490442
38	22.765464104966394	27.98731342325808	24.634600493687728	24.612621978087795
39	24.230965856830558	27.862394171575737	25.519035212683583	22.387604758910122
40	22.854447681507246	27.645068898100973	27.57394620771676	21.926537212675026
41	20.73739791487523	29.11565084213543	25.50021176379994	24.646739479189396
42	20.977557315262825	26.087161124444386	29.894043234039643	23.04123832625315
43	22.59166805703505	25.095507612801654	27.76982772266215	24.542996607501145
44	23.259579638161977	22.829955807675688	28.394263553952708	25.516201000209627
45	21.918141526667263	22.633539535659743	26.65239934802418	28.795919589648815
46	24.729038592348267	24.420751141171586	27.05523184927551	23.794978417204632
47	19.727509187126472	24.86914494483446	32.51403202553144	22.889313842507626
48	21.200925343634893	26.329406077407157	28.153248542252225	24.316420036705725
49	22.704394847508674	23.022896158733012	31.156390774798826	23.116318218959492
50	21.73578936560699	23.92898853908646	28.914528707898572	25.420693387407965
51	20.32296118519279	23.796743115537474	27.18223665353303	28.698059045736702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	308.0
1	1055.0
2	1802.0
3	9826.5
4	17851.0
5	11455.0
6	5059.0
7	4884.5
8	4710.0
9	4753.0
10	4796.0
11	4741.0
12	4686.0
13	4553.0
14	4420.0
15	4328.5
16	4237.0
17	4156.5
18	4076.0
19	4393.5
20	4711.0
21	4775.5
22	4840.0
23	5755.5
24	6671.0
25	8153.5
26	12288.5
27	14941.0
28	17764.5
29	20588.0
30	24380.5
31	28173.0
32	31669.0
33	35165.0
34	40927.5
35	46690.0
36	50358.5
37	54027.0
38	57777.0
39	61527.0
40	66022.5
41	70518.0
42	75864.5
43	81211.0
44	91071.0
45	100931.0
46	162312.5
47	223694.0
48	202564.0
49	181434.0
50	181162.0
51	180890.0
52	163092.0
53	145294.0
54	132732.0
55	120170.0
56	113245.5
57	106321.0
58	96907.5
59	87494.0
60	78486.0
61	69478.0
62	61118.0
63	52758.0
64	45511.0
65	38264.0
66	32124.5
67	25985.0
68	21808.0
69	17631.0
70	14897.5
71	12164.0
72	9614.0
73	7064.0
74	5890.0
75	3631.0
76	2546.0
77	1888.5
78	1231.0
79	1033.0
80	835.0
81	564.5
82	294.0
83	184.0
84	74.0
85	49.0
86	24.0
87	41.0
88	58.0
89	33.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1870008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.358594185600168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.2272972452683	20.482646117012806
2	10.387877296838969	5.8917119342173105
3	3.9583108623459093	3.3675639421716204
4	2.0213022400873673	2.292851598123288
5	1.258745643207301	1.7848128439304052
6	0.8926254265958494	1.5188161339547952
7	0.7352189631577599	1.4594843349624043
8	0.5743939193039582	1.3031203248173855
9	0.5223408097017571	1.333156594401894
>10	7.076789821034812	44.40820360453284
>50	0.2762173183254968	4.880326002371437
>100	0.06124829795109874	3.1189761115243306
>500	0.0024804507589604704	0.513876069371127
>1k	0.0040068819952438365	2.650977178761674
>5k	3.816078090708416E-4	0.7546624765209126
>10k+	7.632156181416832E-4	4.238814733325823
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	28569	1.5277474748771127	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	20605	1.101866943884732	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	17971	0.9610119314997582	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11193	0.5985535890755548	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	7134	0.3814956941360679	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	6813	0.36432999217115647	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC	4956	0.2650256041685383	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT	4700	0.2513358231622539	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	4196	0.22438406680613132	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	4051	0.2166300892830405	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	3760	0.20106865852980307	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA	3663	0.19588151494539058	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT	3034	0.16224529520729322	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	2528	0.1351865874370591	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2489	0.13310103486188293	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1873	0.10015999931551095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3475707055798695E-5	0.0	0.0	0.23775299357008098	0.0
2	5.3475707055798695E-5	0.0	0.0	1.0112256204251533	0.0
3	5.3475707055798695E-5	0.0	0.0	1.4641648591877683	0.0
4	5.3475707055798695E-5	0.0	0.0	2.286300379463617	0.0
5	5.3475707055798695E-5	0.0	0.0	4.708054724899573	0.0
6	5.3475707055798695E-5	0.0	0.0	5.707729592600673	0.0
7	5.3475707055798695E-5	0.0	0.0	6.732805421153278	0.0
8	5.3475707055798695E-5	0.0	0.0	8.079377200525345	0.0
9	5.3475707055798695E-5	0.0	0.0	8.823277761378561	0.0
10	5.3475707055798695E-5	0.0	0.0	10.875621922473059	0.0
11	5.3475707055798695E-5	0.0	0.0	12.365722499582889	0.0
12	5.3475707055798695E-5	0.0	0.0	14.823091665918007	0.0
13	5.3475707055798695E-5	0.0	0.0	15.346137556630774	0.0
14	5.3475707055798695E-5	0.0	0.0	15.64656407887025	0.0
15	5.3475707055798695E-5	0.0	0.0	16.119610183485847	0.0
16	5.3475707055798695E-5	0.0	0.0	16.64495552960201	0.0
17	5.3475707055798695E-5	0.0	0.0	17.237680266608486	0.0
18	5.3475707055798695E-5	0.0	0.0	17.800405131956655	0.0
19	5.3475707055798695E-5	0.0	0.0	18.531043717460033	0.0
20	1.0695141411159739E-4	0.0	0.0	19.089544001950795	0.0
21	1.0695141411159739E-4	0.0	0.0	19.793926015289774	0.0
22	1.0695141411159739E-4	0.0	0.0	20.623815513088715	0.0
23	1.0695141411159739E-4	0.0	0.0	21.323010382843282	0.0
24	1.0695141411159739E-4	0.0	0.0	21.875521388143795	0.0
25	1.0695141411159739E-4	0.0	0.0	22.38434274077972	0.0
26	1.0695141411159739E-4	0.0	0.0	22.837442406663502	0.0
27	1.0695141411159739E-4	0.0	0.0	23.368777031969916	0.0
28	1.0695141411159739E-4	0.0	0.0	23.856528956025855	0.0
29	1.0695141411159739E-4	0.0	0.0	24.41256935799205	0.0
30	1.6042712116739606E-4	0.0	0.0	25.070855311848934	0.0
31	1.6042712116739606E-4	0.0	0.0	25.574756899435723	0.0
32	2.1390282822319478E-4	0.0	0.0	26.08470124191982	0.0
33	2.1390282822319478E-4	0.0	0.0	26.585287335669154	0.0
34	2.1390282822319478E-4	0.0	0.0	27.127851859457287	0.0
35	2.1390282822319478E-4	0.0	0.0	27.726245021411675	0.0
36	2.6737853527899347E-4	0.0	0.0	28.241109128944903	0.0
37	2.6737853527899347E-4	0.0	0.0	28.77474320965472	0.0
38	2.6737853527899347E-4	0.0	0.0	29.472815089561113	0.0
39	2.6737853527899347E-4	0.0	0.0	30.727569079918375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	20	7.0344214E-4	45.000004	12
TCGCGAC	25	3.891748E-5	45.0	33
CGTTTTT	5460	0.0	42.445053	1
TACGGCT	2360	0.0	41.28178	7
CCGATGA	1485	0.0	40.151516	18
CGATGAA	1510	0.0	39.784767	19
TGATACC	2285	0.0	39.682713	4
CTTACGG	165	0.0	39.545452	2
TGGGCGA	1930	0.0	39.520725	6
ACGGCTG	2500	0.0	38.88	8
CGGTAGG	220	0.0	38.863636	31
GATACCT	2335	0.0	38.640255	5
CTACGGC	265	0.0	38.207546	6
TCTTGCG	675	0.0	38.0	1
ACCACCG	2315	0.0	37.90497	14
TTGGGAC	2985	0.0	37.839195	5
TTTCGCG	565	0.0	37.83186	1
TTTGGGA	8375	0.0	37.773136	4
CCAATCG	60	1.5643309E-10	37.500004	40
CAATCGA	90	0.0	37.5	41
>>END_MODULE