FastQCFastQC Report
Sat 14 Jan 2017
SRR2933914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311613
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC75370.5746359635044788RNA PCR Primer, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT67960.5181406405700463RNA PCR Primer, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC54570.41605260088151Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC42030.32044513130016244Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.24222083800633265No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG27470.2094367774640843RNA PCR Primer, Index 13 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG24120.183895707041635RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC16690.12724790010468026No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC16530.12602802808450358No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT15940.12152975001010206Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG15410.11748892394326681Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC207.033651E-445.019
ATCCCCG207.033651E-445.038
ATACGGA253.891108E-545.04
TAGTGCG207.033651E-445.015
CGTTTTT22300.041.973091
GGATCGA700.041.7857138
GCGCGAC1050.040.7142879
TCTTGCG4300.040.2906951
TTTCGCG4150.040.120481
CTATGCG403.4587174E-739.3751
GTCTTCG1600.039.3751
TACGGGT750.039.04
TGGGCGA16700.038.9371266
TTTGTCG3300.038.8636361
CGCGACC1100.038.86363610
TTTAGCG700.038.5714261
TTCCGAT356.2489653E-638.57142613
TACGGGA2800.038.5714264
ATCGCGG700.038.5714262
TTTGGGC32000.038.254
Produced by FastQC (version 0.11.5)