##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933914.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1311613
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.391087157568585	33.0	31.0	34.0	31.0	34.0
2	32.62605890609501	34.0	31.0	34.0	31.0	34.0
3	32.66061025622649	34.0	31.0	34.0	31.0	34.0
4	36.06253597669434	37.0	35.0	37.0	35.0	37.0
5	36.114538358494464	37.0	35.0	37.0	35.0	37.0
6	35.7727027713205	37.0	35.0	37.0	35.0	37.0
7	36.09820732182435	37.0	35.0	37.0	35.0	37.0
8	36.11826887961617	37.0	37.0	37.0	35.0	37.0
9	37.98622002069208	39.0	39.0	39.0	37.0	39.0
10	37.56835667228062	39.0	37.0	39.0	35.0	39.0
11	37.47197534638647	39.0	37.0	39.0	35.0	39.0
12	37.4328532882794	39.0	37.0	39.0	35.0	39.0
13	37.445433218487466	39.0	37.0	39.0	35.0	39.0
14	38.69914982544394	40.0	38.0	41.0	35.0	41.0
15	38.73269706841881	40.0	38.0	41.0	35.0	41.0
16	38.775766937351186	40.0	38.0	41.0	35.0	41.0
17	38.751592886011345	40.0	38.0	41.0	35.0	41.0
18	38.70240840857784	40.0	38.0	41.0	35.0	41.0
19	38.71402768957002	40.0	38.0	41.0	35.0	41.0
20	38.657360822132745	40.0	38.0	41.0	35.0	41.0
21	38.57220308124424	40.0	38.0	41.0	35.0	41.0
22	38.575391521736975	40.0	38.0	41.0	35.0	41.0
23	38.508849027876366	40.0	38.0	41.0	34.0	41.0
24	38.48845657979907	40.0	38.0	41.0	34.0	41.0
25	38.41309593607261	40.0	38.0	41.0	34.0	41.0
26	38.34346869084097	40.0	38.0	41.0	34.0	41.0
27	38.35008954623048	40.0	38.0	41.0	34.0	41.0
28	38.29157304784262	40.0	38.0	41.0	34.0	41.0
29	38.26005689178134	40.0	37.0	41.0	34.0	41.0
30	38.170847650945824	40.0	37.0	41.0	34.0	41.0
31	38.091472865852964	40.0	37.0	41.0	34.0	41.0
32	37.984206469438774	40.0	37.0	41.0	34.0	41.0
33	37.915882962428704	40.0	37.0	41.0	34.0	41.0
34	37.89973719382165	40.0	37.0	41.0	34.0	41.0
35	37.8572490513589	40.0	37.0	41.0	33.0	41.0
36	37.7780473356089	40.0	37.0	41.0	33.0	41.0
37	37.71688981429736	40.0	37.0	41.0	33.0	41.0
38	37.6439597655711	40.0	37.0	41.0	33.0	41.0
39	37.55471392857497	40.0	37.0	41.0	33.0	41.0
40	37.4744920948481	40.0	36.0	41.0	33.0	41.0
41	37.43243167001242	40.0	36.0	41.0	33.0	41.0
42	37.44658142302646	40.0	36.0	41.0	33.0	41.0
43	37.36414552158297	40.0	36.0	41.0	33.0	41.0
44	37.244132224977946	40.0	36.0	41.0	32.0	41.0
45	37.25004402975573	40.0	36.0	41.0	32.0	41.0
46	37.158802177166585	39.0	36.0	41.0	32.0	41.0
47	37.09256465131102	39.0	35.0	41.0	32.0	41.0
48	37.03810880191032	39.0	35.0	41.0	32.0	41.0
49	37.023519132549005	39.0	35.0	41.0	32.0	41.0
50	36.949850298830526	39.0	35.0	41.0	32.0	41.0
51	36.0580956425409	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	23.0
10	16.0
11	29.0
12	24.0
13	18.0
14	24.0
15	18.0
16	29.0
17	67.0
18	121.0
19	229.0
20	432.0
21	751.0
22	1260.0
23	1890.0
24	3061.0
25	5000.0
26	7303.0
27	8490.0
28	9217.0
29	11077.0
30	13567.0
31	17606.0
32	23759.0
33	34171.0
34	60379.0
35	82889.0
36	92312.0
37	151964.0
38	306652.0
39	478982.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.628016038267383	4.765658772823996	58.70092778891335	13.905397399995273
2	20.680566600056572	4.172496003013084	53.10354502433263	22.043392372597708
3	19.19171279943093	4.350063623950053	53.55360155777657	22.90462201884245
4	18.086584991152115	4.372555014322059	53.05558880553944	24.485271188986385
5	18.262551530062602	5.647168791404171	52.037910572707034	24.052369105826184
6	20.820394430369323	6.250090537376497	54.4565355787111	18.472979453543083
7	83.10126538849492	1.855120374683691	10.114950065301274	4.928664171520106
8	85.00449446597433	2.0918517885992287	7.768831202496468	5.134822542929965
9	79.75294541911371	4.778467429035851	10.089713962883868	5.378873188966563
10	36.35454970330425	29.296065226556916	19.048072869055126	15.301312201083702
11	24.093234818502104	26.87515296051503	28.326038244512674	20.70557397647019
12	21.865443541654436	23.313812839610463	32.82355389890158	21.997189719833518
13	22.576705171418705	24.179769489933385	33.62409491214253	19.61943042650538
14	20.34723657054329	26.089555379521244	31.99358347317387	21.56962457676159
15	17.93112755058085	27.04768860936877	33.786490374828546	21.23469346522183
16	19.827723573950546	27.027560721035854	30.85002969625949	22.294686008754105
17	20.12544858887492	26.68142203531072	29.40539625636525	23.787733119449108
18	20.44627493018139	25.48320274349217	31.75830065728229	22.312221669044146
19	21.80795707270361	27.65244016337136	29.591579223444718	20.94802354048031
20	23.204024357794562	27.52290500322885	29.787978618693167	19.485092020283425
21	23.484976132441503	27.49301813873452	30.048726263005932	18.973279465818045
22	22.037293012496825	25.138283929787214	29.318251648923884	23.506171408792078
23	20.343043260473937	26.80135070329434	28.362329437112926	24.493276599118797
24	21.141373255678314	25.639803814082356	29.65966333057083	23.5591595996685
25	19.99461731471097	27.4914932987093	27.942236010164585	24.57165337641515
26	19.35997889621405	28.16516762185187	29.40859842041822	23.066255061515857
27	19.49591838446249	27.203984711953904	29.657071102527954	23.643025801055646
28	17.567300720563154	26.2497398241707	30.608952488272074	25.574006966994073
29	17.637367119722054	25.695994169011744	30.977658806370478	25.688979904895724
30	19.528473719000957	27.811480978001896	29.05933381264138	23.60071149035577
31	19.380640478555794	29.975305215791547	26.733190354166968	23.910863951485688
32	19.5640024915886	29.92879759502231	26.958409225892087	23.548790687496997
33	19.79722677344613	28.383448471462238	25.907641964512397	25.91168279057923
34	18.717640035589767	27.6392502971532	27.643519849223818	25.999589818033215
35	18.472750727539296	26.55790999326783	27.977459814747185	26.991879464445685
36	18.911218476791554	27.27389862711028	27.317051599824033	26.49783129627413
37	17.986174275491322	28.26527336950762	27.540288179516363	26.20826417548469
38	18.84427799968436	29.867117816002132	26.154971016603216	25.133633167710297
39	18.97533799985209	29.701520189263142	27.48912979667021	23.834012014214558
40	20.67904176003135	28.83312379489987	27.605017638586993	22.88281680648179
41	20.017566157090545	27.151072763078744	26.982806666295623	25.84855441353509
42	20.845325564781685	24.865947501282772	26.930275927426763	27.358451006508776
43	20.083972940188914	24.38684276535838	27.903657557526497	27.625526736926215
44	18.76330899434513	25.944238125117696	27.10852972637508	28.183923154162088
45	19.92660944958612	25.42007436644803	26.73989965027794	27.913416533687908
46	20.45900734439198	26.052959218915944	28.161889215797647	25.326144220894424
47	18.049455136537986	26.396505676598203	29.503367227985695	26.050671958878112
48	17.92845908053671	25.652383744290425	28.78349025207893	27.635666923093932
49	19.414873137122	24.806250014295376	29.500012579930207	26.278864268652413
50	19.58352044391143	25.127991259616977	27.41509881344573	27.87338948302586
51	18.709634625457355	24.635239205466856	25.88286331410256	30.772262854973224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	422.5
2	662.0
3	2889.0
4	5116.0
5	3520.5
6	1925.0
7	2124.5
8	2324.0
9	2553.0
10	2782.0
11	2907.5
12	3033.0
13	3204.5
14	3376.0
15	3162.0
16	2948.0
17	2965.5
18	2983.0
19	3087.5
20	3192.0
21	3584.0
22	3976.0
23	4188.0
24	4400.0
25	5452.5
26	8497.5
27	10490.0
28	11660.5
29	12831.0
30	16297.0
31	19763.0
32	22144.0
33	24525.0
34	29787.5
35	35050.0
36	39196.0
37	43342.0
38	48389.5
39	53437.0
40	60847.5
41	68258.0
42	78416.5
43	88575.0
44	99066.5
45	109558.0
46	126534.0
47	143510.0
48	160579.0
49	177648.0
50	167860.5
51	158073.0
52	130249.0
53	102425.0
54	85157.5
55	67890.0
56	56837.0
57	45784.0
58	40170.5
59	34557.0
60	30343.0
61	26129.0
62	22413.0
63	18697.0
64	15007.0
65	11317.0
66	9084.0
67	6851.0
68	5497.0
69	4143.0
70	3276.0
71	2409.0
72	1866.0
73	1323.0
74	1115.5
75	633.5
76	359.0
77	280.0
78	201.0
79	122.0
80	43.0
81	43.0
82	43.0
83	48.0
84	53.0
85	32.0
86	11.0
87	6.5
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1311613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.49584385940507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03803859966294	24.860592823526208
2	13.114770084001037	9.31039662307395
3	5.14003175669254	5.473492940038258
4	2.526225252749826	3.586819885011758
5	1.5253360812786694	2.7071545687092224
6	1.0028298937558104	2.1357775995780015
7	0.7218662441727018	1.7936276043370578
8	0.5627039329708164	1.597892075504419
9	0.46029580293313044	1.4704729155048497
>10	4.679934521680973	35.54501609945513
>50	0.16241738917666138	3.733329474363784
>100	0.05947291739533005	3.7816145408347426
>500	0.0032558161765708213	0.8266960163406155
>1k	0.0021705441177138807	1.652390443845031
>5k	6.511632353141643E-4	1.5247263898770533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	7537	0.5746359635044788	RNA PCR Primer, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	6796	0.5181406405700463	RNA PCR Primer, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	5457	0.41605260088151	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	4203	0.32044513130016244	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.24222083800633265	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	2747	0.2094367774640843	RNA PCR Primer, Index 13 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	2412	0.183895707041635	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	1669	0.12724790010468026	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	1653	0.12602802808450358	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1594	0.12152975001010206	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	1541	0.11748892394326681	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.287260037831281E-4	0.0	0.0	0.546426423037893	0.0
2	2.287260037831281E-4	0.0	0.0	1.9627740804642833	0.0
3	2.287260037831281E-4	0.0	0.0	2.910004704131478	0.0
4	2.287260037831281E-4	0.0	0.0	3.9217360608655145	0.0
5	2.287260037831281E-4	0.0	0.0	5.443221438030883	0.0
6	2.287260037831281E-4	0.0	0.0	6.532338426044878	0.0
7	2.287260037831281E-4	0.0	0.0	7.428486908867174	0.0
8	2.287260037831281E-4	0.0	0.0	8.844529598288519	0.0
9	2.287260037831281E-4	0.0	0.0	9.474517254708516	0.0
10	2.287260037831281E-4	0.0	0.0	10.68432533071874	0.0
11	2.287260037831281E-4	0.0	0.0	13.2009975503445	0.0
12	2.287260037831281E-4	0.0	0.0	15.405839984812593	0.0
13	2.287260037831281E-4	0.0	0.0	16.154155227189726	0.0
14	2.287260037831281E-4	0.0	0.0	16.40377153931838	0.0
15	2.287260037831281E-4	0.0	0.0	16.74144736290354	0.0
16	2.287260037831281E-4	0.0	0.0	17.56120136046227	0.0
17	2.287260037831281E-4	0.0	0.0	18.85899270592774	0.0
18	2.287260037831281E-4	0.0	0.0	20.244386110842147	0.0
19	2.287260037831281E-4	0.0	0.0	21.266943831755253	0.0
20	2.287260037831281E-4	0.0	0.0	22.293237410730146	0.0
21	3.049680050441708E-4	0.0	0.0	23.787885603451628	0.0
22	3.049680050441708E-4	0.0	0.0	25.423810224509822	0.0
23	3.049680050441708E-4	0.0	0.0	27.067054077689075	0.0
24	3.049680050441708E-4	0.0	0.0	28.233251728977983	0.0
25	3.049680050441708E-4	0.0	0.0	29.27121033414582	0.0
26	3.049680050441708E-4	0.0	0.0	30.13617583845235	0.0
27	3.049680050441708E-4	0.0	0.0	30.987875234539455	0.0
28	3.049680050441708E-4	0.0	0.0	31.93213242015747	0.0
29	3.049680050441708E-4	0.0	0.0	32.9655927472509	0.0
30	3.049680050441708E-4	0.0	0.0	34.012776634571324	0.0
31	3.049680050441708E-4	0.0	0.0	35.02061964924105	0.0
32	3.049680050441708E-4	0.0	0.0	35.96312326883006	0.0
33	3.049680050441708E-4	0.0	0.0	36.87520632991591	0.0
34	3.049680050441708E-4	0.0	0.0	37.76998245671551	0.0
35	3.049680050441708E-4	0.0	0.0	38.70425194016833	0.0
36	3.049680050441708E-4	0.0	0.0	39.59422482088848	0.0
37	3.049680050441708E-4	0.0	0.0	40.49426164577509	0.0
38	3.049680050441708E-4	0.0	0.0	41.375314212347696	0.0
39	3.049680050441708E-4	0.0	0.0	42.25293588886356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGC	20	7.033651E-4	45.0	19
ATCCCCG	20	7.033651E-4	45.0	38
ATACGGA	25	3.891108E-5	45.0	4
TAGTGCG	20	7.033651E-4	45.0	15
CGTTTTT	2230	0.0	41.97309	1
GGATCGA	70	0.0	41.785713	8
GCGCGAC	105	0.0	40.714287	9
TCTTGCG	430	0.0	40.290695	1
TTTCGCG	415	0.0	40.12048	1
CTATGCG	40	3.4587174E-7	39.375	1
GTCTTCG	160	0.0	39.375	1
TACGGGT	75	0.0	39.0	4
TGGGCGA	1670	0.0	38.937126	6
TTTGTCG	330	0.0	38.863636	1
CGCGACC	110	0.0	38.863636	10
TTTAGCG	70	0.0	38.571426	1
TTCCGAT	35	6.2489653E-6	38.571426	13
TACGGGA	280	0.0	38.571426	4
ATCGCGG	70	0.0	38.571426	2
TTTGGGC	3200	0.0	38.25	4
>>END_MODULE