##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933912.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	964413
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.345115629922034	33.0	31.0	34.0	31.0	34.0
2	32.570552242659524	34.0	31.0	34.0	31.0	34.0
3	32.641397409616005	34.0	31.0	34.0	31.0	34.0
4	36.03256177591965	37.0	35.0	37.0	35.0	37.0
5	36.073798258629864	37.0	35.0	37.0	35.0	37.0
6	35.71537194127412	37.0	35.0	37.0	35.0	37.0
7	36.03646674194562	37.0	35.0	37.0	35.0	37.0
8	36.056665557183486	37.0	35.0	37.0	35.0	37.0
9	37.96306354227909	39.0	39.0	39.0	35.0	39.0
10	37.52833485239208	39.0	37.0	39.0	35.0	39.0
11	37.44528122287858	39.0	37.0	39.0	35.0	39.0
12	37.14043257401134	39.0	37.0	39.0	34.0	39.0
13	36.964296416576715	39.0	35.0	39.0	33.0	39.0
14	37.71330021474203	40.0	37.0	41.0	32.0	41.0
15	37.915912581020784	40.0	37.0	41.0	33.0	41.0
16	38.14416437770955	40.0	37.0	41.0	33.0	41.0
17	38.218194901976645	40.0	37.0	41.0	34.0	41.0
18	38.229923279756704	40.0	37.0	41.0	34.0	41.0
19	38.24980584044388	40.0	37.0	41.0	34.0	41.0
20	38.135748895960546	40.0	36.0	41.0	33.0	41.0
21	38.062800895466985	40.0	36.0	41.0	34.0	41.0
22	38.148335827078235	40.0	36.0	41.0	34.0	41.0
23	38.1337030919326	40.0	36.0	41.0	34.0	41.0
24	38.1195514784641	40.0	36.0	41.0	34.0	41.0
25	37.99164051085997	40.0	36.0	41.0	34.0	41.0
26	37.89531248541859	40.0	36.0	41.0	34.0	41.0
27	37.90193724058054	40.0	36.0	41.0	34.0	41.0
28	37.85082739448763	40.0	36.0	41.0	34.0	41.0
29	37.82614191223055	40.0	36.0	41.0	34.0	41.0
30	37.772905383896735	40.0	36.0	41.0	34.0	41.0
31	37.64796824596931	40.0	36.0	41.0	33.0	41.0
32	37.57194479958275	40.0	35.0	41.0	33.0	41.0
33	37.55799019714583	40.0	35.0	41.0	33.0	41.0
34	37.534030545005095	40.0	35.0	41.0	33.0	41.0
35	37.471184025930796	40.0	35.0	41.0	33.0	41.0
36	37.36162722816884	40.0	35.0	41.0	33.0	41.0
37	37.25074319819414	39.0	35.0	41.0	32.0	41.0
38	37.19956906429092	39.0	35.0	41.0	32.0	41.0
39	37.18038641121594	39.0	35.0	41.0	32.0	41.0
40	37.0480043300951	39.0	35.0	41.0	32.0	41.0
41	36.886630520326875	39.0	35.0	41.0	31.0	41.0
42	36.8923780579482	39.0	35.0	41.0	31.0	41.0
43	36.837487673849274	39.0	35.0	41.0	31.0	41.0
44	36.74346260367705	39.0	35.0	41.0	31.0	41.0
45	36.763544249196144	39.0	35.0	41.0	31.0	41.0
46	36.64834360382948	39.0	35.0	40.0	31.0	41.0
47	36.53567299486838	39.0	35.0	40.0	31.0	41.0
48	36.47087917728193	38.0	35.0	40.0	31.0	41.0
49	36.48313948484726	38.0	35.0	40.0	31.0	41.0
50	36.38629300932277	38.0	35.0	40.0	31.0	41.0
51	35.24223128472968	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	18.0
10	36.0
11	20.0
12	13.0
13	12.0
14	8.0
15	19.0
16	55.0
17	80.0
18	136.0
19	234.0
20	412.0
21	708.0
22	1155.0
23	1664.0
24	2586.0
25	3924.0
26	5310.0
27	6370.0
28	7658.0
29	9615.0
30	12565.0
31	17278.0
32	23449.0
33	36704.0
34	73103.0
35	73453.0
36	65461.0
37	106373.0
38	211237.0
39	304573.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.939536277507663	4.6951876426385795	58.383182308824125	12.982093771029632
2	19.468319070771546	4.254816142047027	55.17864234513637	21.09822244204506
3	20.71581366074493	4.020165634432551	52.968800710898755	22.295219993923766
4	19.485013163447608	4.733034498705431	51.92059833287191	23.86135400497505
5	17.948741877183323	5.274711145536197	52.35194880201739	24.42459817526309
6	20.47359378191708	6.345206877136662	54.82070440775891	18.360494933187336
7	82.84127235945597	1.7800465153414564	10.217821617916806	5.160859507285779
8	86.063543315986	2.11133611844718	6.770439635301473	5.054680930265353
9	81.6768334727964	3.841092975727204	9.553064921356306	4.929008630120083
10	40.03730766798042	23.099543452856818	19.756058866896236	17.10709001226653
11	31.789803745905544	25.1164179661618	23.956956200300077	19.13682208763258
12	28.78911835489567	20.75065350632976	29.89735725254637	20.5628708862282
13	27.43461566776889	23.512333409027043	30.00177309928423	19.051277823919836
14	20.742047234950174	24.79860806521687	34.083737983623195	20.375606716209756
15	18.713455749766958	24.22406168311709	36.15587927578745	20.90660329132851
16	23.135627578641103	23.649100540950815	30.024895972990823	23.190375907417256
17	24.233186404579783	24.14453144036839	26.518203300867988	25.104078854183843
18	25.169818324721877	22.35546389358086	30.86063750696019	21.61408027473707
19	25.06695782823334	24.853978534092757	28.36658153716302	21.71248210051088
20	22.389681599065963	30.172239486609993	28.67516302662863	18.762915887695417
21	21.812854036600502	27.33351790156292	32.302550878098906	18.55107718373767
22	19.921340753390922	23.491491715686124	32.907478435068796	23.67968909585416
23	19.168965992785246	25.32618286978711	31.37556212950261	24.129289007925028
24	20.817740947083873	27.0133231302357	28.89477848183299	23.274157440847436
25	22.104845123406676	27.172487305749716	26.31714835863888	24.40551921220473
26	18.619201524658006	28.037054664339863	27.119605397272746	26.224138413729385
27	19.87996843675894	26.508352749288942	30.660101014814195	22.95157779913792
28	16.598386790721403	26.583320631306297	30.520326872408397	26.297965705563904
29	18.54340412250768	27.708668381699542	29.920272746219723	23.827654749573057
30	23.955504540067377	25.886731099642997	28.301049446658226	21.8567149136314
31	22.45210298907211	28.230332855322356	28.491009557108832	20.826554598496703
32	23.558993916506722	28.814937169034426	25.02797038198365	22.598098532475195
33	23.845385742415335	25.659235203175403	25.24893380740409	25.246445247005173
34	18.974236141570053	25.86578571628545	29.67566799700958	25.484310145134913
35	19.118261574657332	29.340127103222375	26.188469048011587	25.35314227410871
36	24.521859410854063	27.825734410465223	24.54041992382931	23.111986254851395
37	20.658680461586478	30.608878146603168	27.06413123838024	21.668310153430117
38	19.913149242077825	28.660542734285	27.752736638763682	23.673571384873494
39	19.288727961983092	23.88706912909718	28.511851250449755	28.31235165846997
40	23.990344385652204	23.585953320828317	27.929735497136598	24.493966796382878
41	21.18698109627307	25.56093706741821	25.066750448200096	28.18533138810862
42	19.84077361047601	25.69397135874361	27.784154713800003	26.681100316980384
43	20.136082777814067	24.130014838041376	28.990173297124777	26.743729087019773
44	20.62041884545314	24.235882345011937	27.47775071468344	27.66594809485148
45	20.838375260391555	23.53244927225162	25.651976902011896	29.97719856534493
46	21.298136794091327	24.827019129771166	28.29420590556121	25.580638170576297
47	18.074414177328592	26.523802561765553	31.249786139340717	24.151997121565138
48	17.94729021695062	28.232406655654785	29.066178079308347	24.754125048086244
49	21.629737467246915	23.51326661917664	30.3969357526288	24.460060160947645
50	21.00645677733502	23.63489500867367	26.93648882791916	28.42215938607215
51	18.823470857402377	24.47748008374006	24.323396719040495	32.375652339817066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	288.0
2	444.0
3	1180.0
4	1916.0
5	1334.5
6	753.0
7	818.0
8	883.0
9	1063.0
10	1243.0
11	1272.5
12	1302.0
13	1282.5
14	1263.0
15	1250.5
16	1238.0
17	1368.5
18	1499.0
19	1449.0
20	1399.0
21	1507.5
22	1616.0
23	1868.5
24	2121.0
25	2782.0
26	4393.0
27	5343.0
28	6445.0
29	7547.0
30	8693.5
31	9840.0
32	12082.5
33	14325.0
34	17413.5
35	20502.0
36	22888.5
37	25275.0
38	29259.5
39	33244.0
40	39639.0
41	46034.0
42	52907.0
43	59780.0
44	68856.0
45	77932.0
46	91594.0
47	105256.0
48	123861.5
49	142467.0
50	135421.5
51	128376.0
52	108172.5
53	87969.0
54	74022.5
55	60076.0
56	51748.5
57	43421.0
58	36732.5
59	30044.0
60	24422.0
61	18800.0
62	14998.5
63	11197.0
64	9137.0
65	7077.0
66	5519.0
67	3961.0
68	3425.0
69	2889.0
70	2284.0
71	1679.0
72	1335.5
73	992.0
74	791.0
75	421.5
76	253.0
77	218.5
78	184.0
79	128.5
80	73.0
81	42.5
82	12.0
83	16.0
84	20.0
85	11.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	964413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.68615175811356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.96240832931916	19.581700639948224
2	13.635230079308622	8.095550187823012
3	5.939099808709547	5.289270546838045
4	3.1375332727680734	3.725651555260949
5	1.9863073000564386	2.9482909973862124
6	1.3371367767561895	2.381666716568343
7	0.9548666556757861	1.9842421514407627
8	0.7297396374025314	1.7330529295873847
9	0.5722408896567306	1.5288866903292855
>10	5.166602043431749	34.08614509230637
>50	0.4600365725416158	8.609904185848203
>100	0.10857560648972467	5.566180378039297
>500	0.005640291246215429	1.094210871284548
>1k	0.0038777002317731068	2.2547692647950335
>5k	7.050364057769286E-4	1.1204777925443434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	5499	0.5701914014016816	TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	5208	0.5400176065648223	TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	4928	0.5109844019107996	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	4471	0.4635980643147697	TruSeq Adapter, Index 21 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1844	0.1912043906500638	TruSeq Adapter, Index 14 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1843	0.1911007006334423	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1525	0.15812727534780224	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1318	0.13666344190714974	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.1277461004776999	No Hit
GCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1231	0.1276424104610784	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	1076	0.1115704578847444	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1067	0.11063724773515081	No Hit
TGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1011	0.10483060680434628	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6051349370031304	0.0
2	0.0	0.0	0.0	2.2554652415510783	0.0
3	0.0	0.0	0.0	3.198214872673844	0.0
4	0.0	0.0	0.0	4.1793298099465686	0.0
5	0.0	0.0	0.0	5.728562348288545	0.0
6	0.0	0.0	0.0	6.678362900541573	0.0
7	0.0	0.0	0.0	7.5390937285167245	0.0
8	0.0	0.0	0.0	8.966905257394913	0.0
9	0.0	0.0	0.0	9.575565654963174	0.0
10	0.0	0.0	0.0	10.93587498302076	0.0
11	0.0	0.0	0.0	13.697243815668184	0.0
12	0.0	0.0	0.0	16.115813453364897	0.0
13	0.0	0.0	0.0	16.878764595665963	0.0
14	0.0	0.0	0.0	17.132286686305555	0.0
15	0.0	0.0	0.0	17.469071860292217	0.0
16	0.0	0.0	0.0	18.373456185265027	0.0
17	0.0	0.0	0.0	19.731899093023426	0.0
18	1.0369001662150966E-4	0.0	0.0	21.274702850334865	0.0
19	1.0369001662150966E-4	0.0	0.0	22.36282588476099	0.0
20	1.0369001662150966E-4	0.0	0.0	23.52363562083879	0.0
21	1.0369001662150966E-4	0.0	0.0	25.214197651835885	0.0
22	1.0369001662150966E-4	0.0	0.0	26.95639731111049	0.0
23	1.0369001662150966E-4	0.0	0.0	28.620207317819233	0.0
24	1.0369001662150966E-4	0.0	0.0	29.81564951944862	0.0
25	1.0369001662150966E-4	0.0	0.0	30.901491373509067	0.0
26	1.0369001662150966E-4	0.0	0.0	31.7814048545592	0.0
27	1.0369001662150966E-4	0.0	0.0	32.7922788266023	0.0
28	1.0369001662150966E-4	0.0	0.0	33.770386753393	0.0
29	1.0369001662150966E-4	0.0	0.0	34.89760092408543	0.0
30	1.0369001662150966E-4	0.0	0.0	36.19974015281834	0.0
31	1.0369001662150966E-4	0.0	0.0	37.190083501570385	0.0
32	1.0369001662150966E-4	0.0	0.0	38.09260140624401	0.0
33	1.0369001662150966E-4	0.0	0.0	38.999163221565865	0.0
34	1.0369001662150966E-4	0.0	0.0	39.99469107114898	0.0
35	1.0369001662150966E-4	0.0	0.0	41.07420783419552	0.0
36	1.0369001662150966E-4	0.0	0.0	41.978695849184945	0.0
37	1.0369001662150966E-4	0.0	0.0	42.9112838586788	0.0
38	1.0369001662150966E-4	0.0	0.0	44.04886703103338	0.0
39	1.0369001662150966E-4	0.0	0.0	46.29085257042367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTCG	100	0.0	45.000004	1
CGTATAG	25	3.8903367E-5	45.000004	44
CACGGGT	30	2.1651376E-6	45.000004	4
TCTAGCG	50	2.1827873E-11	45.000004	1
TGTTACG	30	2.1651376E-6	45.000004	1
ATACCCG	25	3.8903367E-5	45.000004	32
CGAACTT	20	7.0327224E-4	45.0	11
TCGCAAA	20	7.0327224E-4	45.0	31
CAAACGC	20	7.0327224E-4	45.0	23
CGAACAC	20	7.0327224E-4	45.0	26
CGACGTT	20	7.0327224E-4	45.0	27
TGGACGC	20	7.0327224E-4	45.0	33
ATTACGA	20	7.0327224E-4	45.0	10
CCCAGCG	45	3.8380676E-10	45.0	23
TTATCCG	35	1.2116652E-7	45.0	1
ACCGTTG	20	7.0327224E-4	45.0	11
GCGGAAT	20	7.0327224E-4	45.0	5
TCGAGCG	55	1.8189894E-12	45.0	18
CGTCCCG	20	7.0327224E-4	45.0	42
TCGACGT	20	7.0327224E-4	45.0	26
>>END_MODULE