FastQCFastQC Report
Sat 14 Jan 2017
SRR2933911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031791
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA231921.1414559863686768No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT169390.8336979541694987No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG109750.540163825905322No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTA98980.48715640535862204No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCGA93110.4582656385425469No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC92780.4566414557402804No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT78560.3866539422607936Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC76850.37823772228541225No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC61770.3040174899878974No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC57770.28433042571799955No Hit
GCTCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC51900.25543965890192444No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC45610.2244817503375101No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG38010.1870763282247042No Hit
GCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG35550.17496878369871705No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.17078528254136374No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCCGATTGGA32770.16128627403113804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC31260.1538544072692516No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC30980.15247631277035878No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG26230.1290979239498551No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG25230.12417615788238062No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG24770.12191214549134237No Hit
TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG24240.11930360947558091No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT21330.10498127021923023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTA207.034567E-445.010
CCCGAAC253.891867E-545.020
CTTGACC104900.043.3913237
CTCAACT102000.043.367652
TTGACCC92650.043.348628
ACTTGAC98850.043.1562966
CGAATAT1150.043.04347614
TCAACTT100800.042.9241073
GCTCAAC109950.042.85131
CCTGCAT81700.042.74173713
GCATTAG77900.042.71822716
CATTAGA75700.042.7113617
ACCCTGC82650.042.4137911
AACTTGA99850.042.341015
TGCATTA78850.042.2606215
CCCTGCA83250.042.16216312
CAACTTG101500.042.1182254
TGACCCT83500.041.820369
ATTAGAA77400.041.74418318
GACTGTA51900.041.53179631
Produced by FastQC (version 0.11.5)