##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933911.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2031791
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.342912238512724	33.0	31.0	34.0	31.0	34.0
2	32.584036940807394	34.0	31.0	34.0	31.0	34.0
3	32.67318538176417	34.0	31.0	34.0	31.0	34.0
4	36.0581427912615	37.0	35.0	37.0	35.0	37.0
5	36.03242213396949	37.0	35.0	37.0	35.0	37.0
6	35.42120375570125	37.0	35.0	37.0	33.0	37.0
7	35.93469603911032	37.0	35.0	37.0	35.0	37.0
8	35.966361697635236	37.0	35.0	37.0	35.0	37.0
9	37.84582567793636	39.0	38.0	39.0	35.0	39.0
10	37.55350131977157	39.0	37.0	39.0	35.0	39.0
11	37.51462675048762	39.0	37.0	39.0	35.0	39.0
12	37.41152854796581	39.0	37.0	39.0	35.0	39.0
13	37.34825875299182	39.0	37.0	39.0	35.0	39.0
14	38.5380538647922	40.0	38.0	41.0	34.0	41.0
15	38.60125475504124	40.0	38.0	41.0	35.0	41.0
16	38.66090754413224	40.0	38.0	41.0	35.0	41.0
17	38.639381215882935	40.0	38.0	41.0	35.0	41.0
18	38.54488232303421	40.0	38.0	41.0	34.0	41.0
19	38.59624242847813	40.0	38.0	41.0	34.0	41.0
20	38.5590033620584	40.0	38.0	41.0	34.0	41.0
21	38.45056996511944	40.0	38.0	41.0	34.0	41.0
22	38.47143431583268	40.0	38.0	41.0	34.0	41.0
23	38.398308192131964	40.0	38.0	41.0	34.0	41.0
24	38.366796092708356	40.0	37.0	41.0	34.0	41.0
25	38.29177410471845	40.0	37.0	41.0	34.0	41.0
26	38.25238816394009	40.0	37.0	41.0	34.0	41.0
27	38.25153915929345	40.0	37.0	41.0	34.0	41.0
28	38.10702724837348	40.0	37.0	41.0	34.0	41.0
29	38.11485974689326	40.0	37.0	41.0	34.0	41.0
30	38.0645543759176	40.0	37.0	41.0	34.0	41.0
31	37.99807509729101	40.0	37.0	41.0	34.0	41.0
32	37.91380117344747	40.0	37.0	41.0	34.0	41.0
33	37.854378723008416	40.0	37.0	41.0	33.0	41.0
34	37.78717151518045	40.0	37.0	41.0	33.0	41.0
35	37.72925217209841	40.0	37.0	41.0	33.0	41.0
36	37.58209382756396	40.0	36.0	41.0	33.0	41.0
37	37.48379631566436	40.0	36.0	41.0	33.0	41.0
38	37.432270346703966	40.0	36.0	41.0	33.0	41.0
39	37.40279241319604	40.0	36.0	41.0	33.0	41.0
40	37.34652186174661	40.0	35.0	41.0	33.0	41.0
41	37.19402586191198	39.0	35.0	41.0	32.0	41.0
42	37.1629901894437	39.0	35.0	41.0	32.0	41.0
43	37.01228718898745	39.0	35.0	41.0	32.0	41.0
44	36.87379656667443	39.0	35.0	41.0	31.0	41.0
45	36.880581713375044	39.0	35.0	41.0	32.0	41.0
46	36.75833193473147	39.0	35.0	41.0	31.0	41.0
47	36.65271870974918	39.0	35.0	41.0	31.0	41.0
48	36.59244233289743	39.0	35.0	40.0	31.0	41.0
49	36.56075354207199	39.0	35.0	40.0	31.0	41.0
50	36.45833454326749	38.0	35.0	40.0	31.0	41.0
51	35.37941550090536	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	33.0
10	49.0
11	41.0
12	29.0
13	27.0
14	28.0
15	64.0
16	77.0
17	113.0
18	270.0
19	466.0
20	907.0
21	1458.0
22	2341.0
23	3356.0
24	5110.0
25	7377.0
26	10500.0
27	12973.0
28	15364.0
29	19361.0
30	24185.0
31	31605.0
32	41447.0
33	60455.0
34	111933.0
35	141164.0
36	149439.0
37	245849.0
38	464078.0
39	681289.0
40	387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.950226179759632	4.3364696467303965	50.52729340763887	15.186010765871098
2	18.993587430990686	5.077539963510026	48.636695408139914	27.292177197359376
3	20.386496445746634	5.183308716300052	53.33324146036674	21.096953377586573
4	18.418774371970343	4.983091272675192	46.20686871828845	30.391265637066017
5	18.51228792725236	11.468403984464938	45.76120280087863	24.25810528740407
6	20.488770744628752	12.38513213219273	48.82992394394896	18.29617317922956
7	77.29003622912002	2.616164753166049	9.506981771255015	10.586817246458914
8	77.32778617485755	3.7066312430756905	13.42982619767486	5.535756384391899
9	71.41920601085447	5.586155268922837	16.01916732577317	6.975471394449527
10	40.05564548715886	28.329980790347037	17.459669818401597	14.154703904092496
11	27.710281224791327	28.08044725072608	24.968857525208055	19.24041399927453
12	24.80914621631851	20.2193532700952	29.143844027264613	25.827656486321676
13	24.404773916214808	20.2826963993836	30.580950501306482	24.73157918309511
14	20.527800349543828	23.039918968043466	29.95175192724055	26.480528755172163
15	18.643502210611228	22.60355518850118	36.73202607945404	22.020916521433552
16	24.806586897963424	22.8936932981788	30.664718959774902	21.63500084408288
17	21.72595508100981	23.607546248605292	27.508784121988928	27.157714548395973
18	21.174569628470643	26.994164261973797	30.967309137603227	20.863956971952334
19	21.688155917611603	24.95561797448655	31.28072720078	22.075498907121847
20	23.288566589772273	25.822291761308126	32.23328580547901	18.65585584344059
21	23.570485350117213	29.594973105009327	28.38594127053422	18.448600274339242
22	25.58309393042887	23.684030493293847	28.43072934174824	22.302146234529044
23	21.248199248840063	28.134783548111002	28.68351124697373	21.933505956075205
24	22.075794213085892	27.724062169780257	27.59885244102371	22.60129117611014
25	20.32079086874585	27.909415879881344	25.8465068503601	25.923286401012703
26	19.877290528405727	25.54362136656772	29.57740240014844	25.001685704878113
27	21.31912189787237	24.21538435793839	30.146014033923763	24.319479710265476
28	20.776743277236683	25.535844976181117	29.291152485664124	24.396259260918075
29	18.714424859643536	26.32623138895684	29.20964803958675	25.74969571181288
30	21.962741246516003	26.187929762460804	29.32334083574541	22.525988155277783
31	24.940754240962775	25.070098253216006	27.132761194433876	22.856386311387343
32	23.276262174603588	27.303398823993213	26.097467702140626	23.32287129926257
33	22.51166581602143	24.47141462876841	27.549536345027615	25.467383210182543
34	21.86027007699119	24.911272862218603	29.497374483891303	23.731082576898903
35	24.52968833900731	24.562959477623437	27.239661953419418	23.667690229949834
36	22.35978011517917	26.488009839594724	27.894158405072172	23.258051640153933
37	22.584409518498706	28.473499488874594	26.734442666593168	22.20764832603353
38	21.401512261841894	28.54565257942377	27.261809900723055	22.79102525801128
39	21.23062854397918	26.129754487543256	29.43344074267481	23.206176225802754
40	24.312293931806963	21.2989918746564	32.277926223711	22.110787969825637
41	22.325573841010222	26.338978763071598	26.518524789213068	24.81692260670512
42	23.043314986630023	23.136877759572712	27.2151515584034	26.60465569539387
43	22.102174879207556	24.85221166940891	28.304633695099547	24.740979756283988
44	21.6653681407192	23.898521058514387	29.14349950363989	25.292611297126527
45	23.058572461439194	22.38980288819076	28.27126412116207	26.280360529207975
46	22.77138741140206	23.38306449826778	28.911585886540497	24.93396220378966
47	20.97558262636265	22.90078064131596	31.918145124178622	24.20549160814277
48	19.904901636044258	23.743879168674336	30.81680153125986	25.534417664021547
49	22.48060947213567	22.740626373480342	30.549648069117346	24.229116085266643
50	21.87897278804759	22.16290947248019	31.544779950300004	24.413337789172214
51	20.897080457586434	22.892955033268677	27.688477801112416	28.521486708032466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	960.5
2	1619.0
3	3940.5
4	6262.0
5	4264.0
6	2266.0
7	2297.0
8	2328.0
9	2557.5
10	2787.0
11	2815.5
12	2844.0
13	2868.0
14	2892.0
15	2999.5
16	3107.0
17	2973.5
18	2840.0
19	3185.5
20	3531.0
21	4073.5
22	4616.0
23	6154.0
24	7692.0
25	8616.0
26	12997.0
27	16454.0
28	18728.0
29	21002.0
30	36073.5
31	51145.0
32	45102.5
33	39060.0
34	44667.5
35	50275.0
36	54334.5
37	58394.0
38	72424.0
39	86454.0
40	91441.5
41	96429.0
42	99251.0
43	102073.0
44	114023.0
45	125973.0
46	162284.5
47	198596.0
48	220693.0
49	242790.0
50	234354.0
51	225918.0
52	189691.0
53	153464.0
54	133401.0
55	113338.0
56	102650.5
57	91963.0
58	84872.5
59	77782.0
60	71816.5
61	65851.0
62	61185.5
63	56520.0
64	49630.5
65	42741.0
66	34712.5
67	26684.0
68	21277.5
69	15871.0
70	12151.0
71	8431.0
72	7210.5
73	5990.0
74	4458.0
75	2320.0
76	1714.0
77	1272.0
78	830.0
79	539.5
80	249.0
81	194.0
82	139.0
83	104.0
84	69.0
85	48.5
86	28.0
87	16.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2031791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.979565898685923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.33532731279695	17.493425732323942
2	13.135473342830261	6.825077906409821
3	5.489291052485588	4.278281959053491
4	2.794020535209996	2.9034976246707918
5	1.6844339507750823	2.1880431413072565
6	1.0801994035026043	1.6837866952210279
7	0.7721253550825061	1.4041637081088567
8	0.5824643637179872	1.2105737056678092
9	0.4507337155877592	1.0538879640184575
>10	6.028891443178548	39.12880250770108
>50	0.5543219419677656	8.823979851835924
>100	0.07966422697399374	3.7434022116122403
>500	0.006910600177614839	1.2105106428729
>1k	0.004031183436941989	2.4531878644403586
>5k	0.001535688928358853	3.0506882975248644
>10k+	5.758833481345699E-4	2.54869018723118
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	23192	1.1414559863686768	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	16939	0.8336979541694987	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	10975	0.540163825905322	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	9898	0.48715640535862204	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCGA	9311	0.4582656385425469	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	9278	0.4566414557402804	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	7856	0.3866539422607936	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	7685	0.37823772228541225	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	6177	0.3040174899878974	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	5777	0.28433042571799955	No Hit
GCTCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	5190	0.25543965890192444	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	4561	0.2244817503375101	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	3801	0.1870763282247042	No Hit
GCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	3555	0.17496878369871705	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3470	0.17078528254136374	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCCGATTGGA	3277	0.16128627403113804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	3126	0.1538544072692516	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	3098	0.15247631277035878	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	2623	0.1290979239498551	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	2523	0.12417615788238062	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	2477	0.12191214549134237	No Hit
TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	2424	0.11930360947558091	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT	2133	0.10498127021923023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.46088303373723E-4	0.0	0.0	0.4096385897958993	0.0
2	2.46088303373723E-4	0.0	0.0	1.6248718495160182	0.0
3	2.46088303373723E-4	0.0	0.0	2.388976031491428	0.0
4	2.46088303373723E-4	0.0	0.0	3.162136263030991	0.0
5	2.46088303373723E-4	0.0	0.0	4.772931861594032	0.0
6	2.46088303373723E-4	0.0	0.0	5.6460039443033265	0.0
7	2.9530596404846757E-4	0.0	0.0	6.419164175842889	0.0
8	2.9530596404846757E-4	0.0	0.0	8.028335591603664	0.0
9	2.9530596404846757E-4	0.0	0.0	8.749374320488672	0.0
10	2.9530596404846757E-4	0.0	0.0	10.10546852505991	0.0
11	2.9530596404846757E-4	0.0	0.0	12.14322732997636	0.0
12	2.9530596404846757E-4	0.0	0.0	14.164941177512844	0.0
13	2.9530596404846757E-4	0.0	0.0	15.41561115291878	0.0
14	3.445236247232122E-4	0.0	0.0	15.664357209968939	0.0
15	3.445236247232122E-4	0.0	0.0	16.597327185719397	0.0
16	3.445236247232122E-4	0.0	0.0	17.36778044592185	0.0
17	3.445236247232122E-4	0.0	0.0	18.359959267464024	0.0
18	3.445236247232122E-4	0.0	0.0	19.393037965026913	0.0
19	3.445236247232122E-4	0.0	0.0	20.201339606288244	0.0
20	3.445236247232122E-4	0.0	0.0	21.248396119482763	0.0
21	3.445236247232122E-4	0.0	0.0	22.390442717779536	0.0
22	3.445236247232122E-4	0.0	0.0	23.61620855688405	0.0
23	3.445236247232122E-4	0.0	0.0	24.826077091590623	0.0
24	3.445236247232122E-4	0.0	0.0	25.71061688923713	0.0
25	3.445236247232122E-4	0.0	0.0	27.01547550904596	0.0
26	3.445236247232122E-4	0.0	0.0	27.754232595773875	0.0
27	3.445236247232122E-4	0.0	0.0	28.481079008618504	0.0
28	3.445236247232122E-4	0.0	0.0	29.18484233860668	0.0
29	3.445236247232122E-4	0.0	0.0	29.926650920296428	0.0
30	3.445236247232122E-4	0.0	0.0	30.792488006886536	0.0
31	3.445236247232122E-4	0.0	0.0	31.526028021582928	0.0
32	3.9374128539795676E-4	0.0	0.0	32.43419229635332	0.0
33	3.9374128539795676E-4	0.0	0.0	33.12560199351213	0.0
34	3.9374128539795676E-4	0.0	0.0	34.090415795719146	0.0
35	3.9374128539795676E-4	0.0	0.0	34.82976349437516	0.0
36	4.92176606747446E-4	0.0	0.0	35.521517715158694	0.0
37	5.413942674221906E-4	0.0	0.0	36.24654307455836	0.0
38	5.413942674221906E-4	0.0	0.0	36.998047535401035	0.0
39	5.413942674221906E-4	0.0	0.0	37.969358068817115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCTA	20	7.034567E-4	45.0	10
CCCGAAC	25	3.891867E-5	45.0	20
CTTGACC	10490	0.0	43.391323	7
CTCAACT	10200	0.0	43.36765	2
TTGACCC	9265	0.0	43.34862	8
ACTTGAC	9885	0.0	43.156296	6
CGAATAT	115	0.0	43.043476	14
TCAACTT	10080	0.0	42.924107	3
GCTCAAC	10995	0.0	42.8513	1
CCTGCAT	8170	0.0	42.741737	13
GCATTAG	7790	0.0	42.718227	16
CATTAGA	7570	0.0	42.71136	17
ACCCTGC	8265	0.0	42.41379	11
AACTTGA	9985	0.0	42.34101	5
TGCATTA	7885	0.0	42.26062	15
CCCTGCA	8325	0.0	42.162163	12
CAACTTG	10150	0.0	42.118225	4
TGACCCT	8350	0.0	41.82036	9
ATTAGAA	7740	0.0	41.744183	18
GACTGTA	5190	0.0	41.531796	31
>>END_MODULE