##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933910.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436444
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.423042596857236	33.0	31.0	34.0	31.0	34.0
2	32.64077889566179	34.0	31.0	34.0	31.0	34.0
3	32.70496865871555	34.0	31.0	34.0	31.0	34.0
4	36.08341501652692	37.0	35.0	37.0	35.0	37.0
5	36.09323579617444	37.0	35.0	37.0	35.0	37.0
6	35.67570542255737	37.0	35.0	37.0	35.0	37.0
7	36.03529897441181	37.0	35.0	37.0	35.0	37.0
8	36.03552244292155	37.0	36.0	37.0	35.0	37.0
9	37.91223117643291	39.0	38.0	39.0	35.0	39.0
10	37.60013616959659	39.0	37.0	39.0	35.0	39.0
11	37.48833577918805	39.0	37.0	39.0	35.0	39.0
12	37.115938386738364	39.0	35.0	39.0	34.0	39.0
13	36.919407230633425	39.0	35.0	39.0	33.0	39.0
14	37.64055403482489	40.0	37.0	41.0	32.0	41.0
15	37.841334573432725	40.0	37.0	41.0	33.0	41.0
16	38.09934254311341	40.0	37.0	41.0	33.0	41.0
17	38.1480844362885	40.0	36.0	41.0	33.0	41.0
18	38.13259410043134	40.0	36.0	41.0	34.0	41.0
19	38.14540699115315	40.0	36.0	41.0	34.0	41.0
20	38.03037361707104	40.0	36.0	41.0	33.0	41.0
21	37.990521732834694	40.0	36.0	41.0	33.0	41.0
22	38.06253010907491	40.0	36.0	41.0	34.0	41.0
23	38.04704116554492	40.0	36.0	41.0	34.0	41.0
24	37.998849241599395	40.0	36.0	41.0	34.0	41.0
25	37.90129026958238	40.0	35.0	41.0	34.0	41.0
26	37.84749701345824	40.0	35.0	41.0	34.0	41.0
27	37.821852435597904	40.0	35.0	41.0	34.0	41.0
28	37.71294321254431	40.0	35.0	41.0	34.0	41.0
29	37.66914408079953	40.0	35.0	41.0	33.0	41.0
30	37.59521777389164	40.0	35.0	41.0	33.0	41.0
31	37.44710827571419	40.0	35.0	41.0	33.0	41.0
32	37.39573488420015	39.0	35.0	41.0	33.0	41.0
33	37.33568242131263	39.0	35.0	41.0	33.0	41.0
34	37.22397949380554	39.0	35.0	41.0	32.0	41.0
35	37.19284288144891	39.0	35.0	41.0	32.0	41.0
36	37.08201781621838	39.0	35.0	41.0	32.0	41.0
37	36.945174333284136	39.0	35.0	41.0	31.0	41.0
38	36.85680193589169	39.0	35.0	41.0	31.0	41.0
39	36.86115991991334	39.0	35.0	41.0	31.0	41.0
40	36.78521821943633	39.0	35.0	41.0	31.0	41.0
41	36.639873883005535	39.0	35.0	41.0	31.0	41.0
42	36.63713447931141	39.0	35.0	41.0	31.0	41.0
43	36.54338839523156	39.0	35.0	41.0	31.0	41.0
44	36.46301770204756	38.0	35.0	41.0	31.0	41.0
45	36.50056946180986	38.0	35.0	41.0	31.0	41.0
46	36.44219823397223	38.0	35.0	40.0	31.0	41.0
47	36.308037765481984	38.0	35.0	40.0	31.0	41.0
48	36.21634675629541	38.0	35.0	40.0	31.0	41.0
49	36.21148753449491	38.0	35.0	40.0	31.0	41.0
50	36.134481399901425	38.0	35.0	40.0	31.0	41.0
51	35.10066316542796	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	26.0
10	35.0
11	24.0
12	38.0
13	15.0
14	26.0
15	54.0
16	93.0
17	169.0
18	297.0
19	501.0
20	855.0
21	1507.0
22	2282.0
23	3370.0
24	4552.0
25	6448.0
26	8536.0
27	10466.0
28	12759.0
29	15524.0
30	19816.0
31	26201.0
32	36649.0
33	57807.0
34	120466.0
35	108153.0
36	99471.0
37	160586.0
38	292973.0
39	446418.0
40	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.624350131296453	4.437346669971124	55.37549671271557	15.562806486016859
2	20.070674526817616	5.9604829704464635	52.3100796132672	21.658762889468715
3	21.2129397317264	6.1330619223582685	50.64708404922155	22.00691429669378
4	19.997507734377393	4.800674443277984	50.62334487108443	24.578472951260196
5	20.485031090665558	5.566454383185143	48.57021923583516	25.378295290314135
6	22.29255021427915	7.654179348446581	51.911874044515486	18.141396392758786
7	82.46356975976788	2.4745830676308995	10.219681379851913	4.842165792749317
8	83.5293962034023	4.077430098214758	7.550311742051901	4.842861956331051
9	78.49968394173389	4.98049349643982	9.718930915510802	6.800891646315485
10	44.66703888212837	24.13606099506838	16.84583596715222	14.351064155651038
11	34.26468417842951	22.201700866862893	24.674473909181284	18.859141045526314
12	31.53168518925903	19.002202661572607	28.685211536265946	20.780900612902418
13	29.44089710423797	19.3845357006608	31.845585348262794	19.328981846838442
14	21.74668834984169	21.34973587553709	35.00352258772357	21.900053186897644
15	19.50288350955554	21.851460968892628	37.38690822614735	21.258747295404483
16	26.497378247951193	22.26449482193528	28.68904043596548	22.54908649414805
17	27.123159691571686	22.749511989329203	27.1644421919685	22.96288612713061
18	27.124203936944287	21.597500494276144	30.217815661452867	21.060479907326705
19	26.26381536627951	24.718958762054072	27.40621980390464	21.611006067761778
20	23.733191130319035	29.294354670283006	28.573129199606807	18.39932499979115
21	24.564619295983693	25.443316968848073	31.571157664343335	18.4209060708249
22	22.775061192778836	21.73694205969742	33.21605297526391	22.27194377225983
23	22.186106802632054	23.263071863574215	31.982451108431654	22.568370225362074
24	23.02449660411405	25.7178142691257	28.00123081721251	23.256458309547746
25	24.391970727713716	25.294546811431562	25.772880808440846	24.540601652413876
26	22.07019556627338	24.061153793673824	26.65095193408166	27.217698705971134
27	21.744251777305625	23.18941775662678	31.32353227832063	23.742798187746963
28	19.37757406484346	24.001840656510105	29.647657688012895	26.97292759063354
29	22.728139767369978	26.232557621459662	27.244222538435192	23.79508007273517
30	27.08668071988884	23.382951232348773	28.346388721036114	21.183979326726277
31	24.824357928328567	23.427575317937908	29.838824207556996	21.90924254617653
32	28.01571101971257	24.73636285159742	25.650007936264828	21.597918192425183
33	28.253033184725613	23.238497289139012	26.223089796748077	22.28537972938729
34	22.676832511396196	22.848645683368098	30.673245876623106	23.801275928612604
35	23.449156388971655	26.282820632060837	27.128937849300076	23.13908512966743
36	27.68071710418227	25.368340151095342	25.693935858272233	21.25700688645015
37	23.14305326208331	27.540022444313877	27.93419026429154	21.38273402931127
38	23.57140271392411	26.19440785718065	27.841182809771908	22.393006619123334
39	23.2960700173484	24.7939355798068	26.56546304624475	25.344531356600047
40	25.800309653561154	21.69120411237751	28.36887480472611	24.13961142933522
41	21.902350526717367	22.690198852165487	27.50222076182573	27.90522985929142
42	20.83269518338341	23.142426714859752	31.44877210667454	24.5761059950823
43	21.6025824884228	23.487097304176146	30.36825661146554	24.542063595935517
44	23.25367365522081	21.983801665780216	28.25999482054295	26.50252985845602
45	22.81864103299537	21.440376373878827	26.218843198899506	29.5221393942263
46	23.690655535475102	21.85668219575563	29.231282249777923	25.22138001899134
47	19.741110687224843	23.29126648863443	33.06220082370075	23.905422000439973
48	20.604910459440116	26.041669567348258	29.81257884052563	23.540841132685994
49	23.8410268691296	22.407974136130612	30.881816485710544	22.869182509029244
50	22.15735524670645	22.8452344818176	27.82148138040884	27.17592889106711
51	20.786678770630807	23.31925226462013	26.38181509338338	29.51225387136568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	782.5
2	1400.0
3	2808.5
4	4217.0
5	2976.5
6	1736.0
7	1766.0
8	1796.0
9	1904.0
10	2012.0
11	2080.0
12	2148.0
13	2079.0
14	2010.0
15	2007.0
16	2004.0
17	1987.0
18	1970.0
19	1999.0
20	2028.0
21	2753.0
22	3478.0
23	4007.5
24	4537.0
25	5712.5
26	8378.0
27	9868.0
28	11807.0
29	13746.0
30	16302.5
31	18859.0
32	22410.5
33	25962.0
34	28526.5
35	31091.0
36	34036.0
37	36981.0
38	41146.5
39	45312.0
40	48935.5
41	52559.0
42	59166.5
43	65774.0
44	74072.5
45	82371.0
46	106226.0
47	130081.0
48	144807.0
49	159533.0
50	158297.5
51	157062.0
52	138742.0
53	120422.0
54	111577.0
55	102732.0
56	95458.5
57	88185.0
58	81549.0
59	74913.0
60	64724.5
61	54536.0
62	48719.0
63	42902.0
64	36594.0
65	30286.0
66	25229.0
67	20172.0
68	17144.0
69	14116.0
70	12202.0
71	10288.0
72	8055.5
73	5823.0
74	4844.5
75	2654.5
76	1443.0
77	1003.5
78	564.0
79	439.5
80	315.0
81	236.5
82	158.0
83	120.5
84	83.0
85	60.0
86	37.0
87	22.0
88	7.0
89	7.0
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1436444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.14416936002665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.20409146476602	18.78487579126384
2	12.328184862109229	6.692766755976194
3	4.860701095255255	3.9581908121422704
4	2.532917827193898	2.7501580190552755
5	1.4412536293653735	1.956081630312339
6	0.9978031370867942	1.62507224046299
7	0.6993403975634422	1.3288109934239312
8	0.5508749216271854	1.1962433735071782
9	0.46732468356532547	1.141662632113625
>10	6.311488478088563	43.51483085204876
>50	0.5154888284424058	8.48603511724785
>100	0.08119226727779753	4.028272431706061
>500	0.004669203829428273	0.9335227344161775
>1k	0.0041504034039362425	2.8294371368064324
>5k	5.188004254920303E-4	0.7740394795170493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	5723	0.3984144178262431	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	5270	0.3668782075737028	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	4947	0.3443921238837017	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	4759	0.3313042485471066	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	4488	0.31243821548212114	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	4182	0.2911356098810674	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	3914	0.27247842589060206	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2968	0.20662135105858634	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2546	0.17724324790942075	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2272	0.15816836576991516	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	1705	0.11869589068560975	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	1662	0.11570238728415448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.961635817337814E-5	0.0	0.0	0.38337728446079344	0.0
2	6.961635817337814E-5	0.0	0.0	1.4613169744173806	0.0
3	6.961635817337814E-5	0.0	0.0	2.091345015886453	0.0
4	6.961635817337814E-5	0.0	0.0	2.830322657896862	0.0
5	6.961635817337814E-5	0.0	0.0	4.261217283792476	0.0
6	6.961635817337814E-5	0.0	0.0	5.010219681379852	0.0
7	6.961635817337814E-5	0.0	0.0	5.773214966960077	0.0
8	6.961635817337814E-5	0.0	0.0	6.919866002433788	0.0
9	6.961635817337814E-5	0.0	0.0	7.449646488133196	0.0
10	6.961635817337814E-5	0.0	0.0	8.693064261467903	0.0
11	6.961635817337814E-5	0.0	0.0	10.647543517185493	0.0
12	6.961635817337814E-5	0.0	0.0	12.702131095956403	0.0
13	6.961635817337814E-5	0.0	0.0	13.279181088855536	0.0
14	6.961635817337814E-5	0.0	0.0	13.52966074556335	0.0
15	6.961635817337814E-5	0.0	0.0	13.882337216069683	0.0
16	1.3923271634675628E-4	0.0	0.0	14.5394460208682	0.0
17	1.3923271634675628E-4	0.0	0.0	15.402480013143569	0.0
18	1.3923271634675628E-4	0.0	0.0	16.285215434782003	0.0
19	1.3923271634675628E-4	0.0	0.0	17.03916059379969	0.0
20	1.3923271634675628E-4	0.0	0.0	17.834736335005054	0.0
21	1.3923271634675628E-4	0.0	0.0	18.918314949973684	0.0
22	1.3923271634675628E-4	0.0	0.0	20.067263325267117	0.0
23	1.3923271634675628E-4	0.0	0.0	21.103781282110546	0.0
24	2.0884907452013444E-4	0.0	0.0	21.867333498556157	0.0
25	2.0884907452013444E-4	0.0	0.0	22.561130124112044	0.0
26	2.0884907452013444E-4	0.0	0.0	23.183569982540217	0.0
27	2.0884907452013444E-4	0.0	0.0	23.92073759923812	0.0
28	2.0884907452013444E-4	0.0	0.0	24.566498937654373	0.0
29	2.0884907452013444E-4	0.0	0.0	25.348986803523143	0.0
30	2.0884907452013444E-4	0.0	0.0	26.288877255221923	0.0
31	2.0884907452013444E-4	0.0	0.0	26.966522885681584	0.0
32	2.0884907452013444E-4	0.0	0.0	27.618201614542578	0.0
33	2.0884907452013444E-4	0.0	0.0	28.26674760728577	0.0
34	2.0884907452013444E-4	0.0	0.0	28.983865712829736	0.0
35	2.0884907452013444E-4	0.0	0.0	29.767537056787457	0.0
36	2.0884907452013444E-4	0.0	0.0	30.442049951129317	0.0
37	2.0884907452013444E-4	0.0	0.0	31.152345653572294	0.0
38	2.0884907452013444E-4	0.0	0.0	32.08736296019894	0.0
39	2.0884907452013444E-4	0.0	0.0	33.99234498525526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGC	20	7.0338754E-4	45.0	13
ACGGGAC	55	1.8189894E-12	45.0	5
GCCGAAT	20	7.0338754E-4	45.0	17
CGAATTG	25	3.891294E-5	45.0	31
CTATGCG	45	1.9288564E-8	40.0	1
TACGAAT	130	0.0	39.80769	12
TTGTTCG	375	0.0	39.600002	1
TTGGGAC	2085	0.0	39.496403	5
CGTTTTT	1400	0.0	39.375	1
TTTCGCG	540	0.0	38.75	1
CTATCCG	35	6.249378E-6	38.57143	1
TAAGACG	35	6.249378E-6	38.57143	14
TTTGGGA	6005	0.0	38.55537	4
CGAATAT	135	0.0	38.333332	14
GTTTGCG	215	0.0	37.67442	1
CGAACGA	90	0.0	37.5	22
TCCGATA	90	0.0	37.5	14
ATAATCG	30	1.1400641E-4	37.499996	26
CTGCGTA	30	1.1400641E-4	37.499996	1
CGGTCTA	30	1.1400641E-4	37.499996	31
>>END_MODULE