FastQCFastQC Report
Sat 14 Jan 2017
SRR2933909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376173
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC348452.5320217734252886No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC339382.4661143620751167No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG266341.9353671377072506No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC110220.8009167452057262No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT86280.6269560585769376No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT82350.5983986025012844No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT77920.5662078822938686No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC72900.5297299104109731No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC72740.5285672658888091No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC67570.4909993147663848No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT64310.4673104326272932No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA54800.39820574884117044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.2404494202400425No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG31240.22700634295252123No Hit
TCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG27590.20048351479065493No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT22280.16189824971133718No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC20120.14620254866212315No Hit
TTCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT18960.13777337587643415No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG18500.13443077287521266No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC17890.12999819063446238No Hit
TGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG16150.11735443145592886No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC15960.11597379108585912No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT15170.11023323375767435No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT14870.10805327527861686No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG14440.1049286681253011No Hit
GCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG14380.1044926764294896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG302.165816E-645.0000041
TGCCGCG302.165816E-645.0000041
CGGGTTC453.8562575E-1045.06
TCGGCCG207.033772E-445.01
TATTGCG406.8175723E-945.01
TACGATT207.033772E-445.042
CGTACAA207.033772E-445.024
CCGCGCA406.8175723E-945.040
CGCCGGA207.033772E-445.039
ACGAGTA207.033772E-445.014
TCGACTC207.033772E-445.027
TCGCGGA207.033772E-445.03
CACGGGT253.8912094E-544.9999964
ACGTTCC253.8912094E-544.99999610
ATGTCGG253.8912094E-544.9999962
GCGATCG253.8912094E-544.9999969
TTACGCG253.8912094E-544.9999961
CGTTTTT20200.043.2178231
TCGGGCG2000.042.7499965
CTTTGCG2350.042.127661
Produced by FastQC (version 0.11.5)