##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933909.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1376173
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16936606080776	33.0	31.0	34.0	30.0	34.0
2	32.359450447000484	34.0	31.0	34.0	30.0	34.0
3	32.449149925191094	34.0	31.0	34.0	30.0	34.0
4	35.93278025364543	37.0	35.0	37.0	35.0	37.0
5	35.955220746228854	37.0	35.0	37.0	35.0	37.0
6	35.29243271013165	37.0	35.0	37.0	33.0	37.0
7	35.83161346720216	37.0	35.0	37.0	35.0	37.0
8	35.720806904364494	37.0	35.0	37.0	35.0	37.0
9	37.709923824984216	39.0	38.0	39.0	35.0	39.0
10	37.36232871884567	39.0	37.0	39.0	34.0	39.0
11	37.28939239470619	39.0	37.0	39.0	34.0	39.0
12	37.25935983339304	39.0	37.0	39.0	34.0	39.0
13	37.19363408524946	39.0	37.0	39.0	33.0	39.0
14	38.37585172794409	40.0	38.0	41.0	34.0	41.0
15	38.31413928336045	40.0	38.0	41.0	33.0	41.0
16	38.487876160918724	40.0	38.0	41.0	34.0	41.0
17	38.34064539850731	40.0	38.0	41.0	34.0	41.0
18	38.3392276988431	40.0	38.0	41.0	34.0	41.0
19	38.382631398813956	40.0	38.0	41.0	34.0	41.0
20	38.192502686798825	40.0	37.0	41.0	34.0	41.0
21	38.258375945466156	40.0	37.0	41.0	34.0	41.0
22	38.2891307996887	40.0	37.0	41.0	34.0	41.0
23	38.15338841846192	40.0	37.0	41.0	34.0	41.0
24	38.10874141550517	40.0	37.0	41.0	34.0	41.0
25	38.04879691724805	40.0	37.0	41.0	34.0	41.0
26	37.98466617205831	40.0	37.0	41.0	33.0	41.0
27	37.95348985919648	40.0	37.0	41.0	33.0	41.0
28	37.91131565580781	40.0	37.0	41.0	33.0	41.0
29	37.8972055112257	40.0	37.0	41.0	33.0	41.0
30	37.878536346811046	40.0	37.0	41.0	33.0	41.0
31	37.859438457228855	40.0	37.0	41.0	33.0	41.0
32	37.61155973849218	40.0	37.0	41.0	33.0	41.0
33	37.53614044164505	40.0	37.0	41.0	33.0	41.0
34	37.51454431964586	40.0	37.0	41.0	33.0	41.0
35	37.47160131756691	40.0	37.0	41.0	33.0	41.0
36	37.382435202550845	40.0	36.0	41.0	32.0	41.0
37	37.41572534848453	40.0	37.0	41.0	33.0	41.0
38	37.296945950836125	40.0	36.0	41.0	32.0	41.0
39	37.268179945399304	40.0	36.0	41.0	32.0	41.0
40	37.13236271893141	40.0	36.0	41.0	31.0	41.0
41	37.015629575642016	39.0	36.0	41.0	31.0	41.0
42	37.08303679842578	39.0	36.0	41.0	31.0	41.0
43	36.96397327952227	39.0	36.0	41.0	31.0	41.0
44	36.97375039330084	39.0	36.0	41.0	31.0	41.0
45	37.03636534069481	39.0	36.0	41.0	31.0	41.0
46	36.915286086851	39.0	35.0	41.0	31.0	41.0
47	36.71149630170044	39.0	35.0	41.0	31.0	41.0
48	36.73451375662798	39.0	35.0	41.0	31.0	41.0
49	36.83185471594051	39.0	35.0	41.0	31.0	41.0
50	36.77697789449437	39.0	35.0	41.0	31.0	41.0
51	35.83908563821554	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	9.0
11	10.0
12	7.0
13	10.0
14	12.0
15	21.0
16	15.0
17	51.0
18	168.0
19	422.0
20	982.0
21	1939.0
22	3018.0
23	4390.0
24	5670.0
25	7453.0
26	8864.0
27	9867.0
28	10749.0
29	13115.0
30	16987.0
31	23143.0
32	31555.0
33	43557.0
34	67977.0
35	90373.0
36	102646.0
37	161057.0
38	314473.0
39	457414.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.768244254174437	4.0564667378302	52.330411946753784	11.844877061241574
2	16.95789700858831	16.295407626802735	47.56945529377484	19.177240070834117
3	16.735686574289716	16.23138951280108	48.01293151369777	19.019992399211436
4	16.262853580182142	3.7080367075941765	58.6431357104085	21.38597400181518
5	24.609769265928048	4.578421462999201	47.67990652338042	23.131902747692333
6	18.41672522277359	13.86795119508957	50.89229333811956	16.823030244017286
7	74.60500968991543	1.218088132814697	19.154205176238744	5.022697001031121
8	73.79588176777193	9.69921659558791	10.147779385295308	6.357122251344853
9	69.78221488141389	3.931773112828111	10.91003820013908	15.375973805618914
10	37.021580862289845	29.594607654706202	19.216987980435597	14.166823502568354
11	24.7191305162941	23.351206570685516	30.447189415865594	21.482473497154793
12	19.320754004038736	20.325278871188434	34.51114067780722	25.842826446965606
13	21.50158446648786	22.738638238070358	38.55874225115593	17.201035044285856
14	19.76510220735329	29.452765023002197	29.859981266890134	20.922151502754378
15	14.48095551940054	25.320435730100794	38.987685414551805	21.21092333594686
16	16.068982606111295	27.91923689826788	28.54706494023644	27.464715555384387
17	17.574461931748406	27.772598357909946	31.480199073808308	23.172740636533344
18	17.125317819779927	25.8312726670266	32.35923099784693	24.684178515346545
19	17.574970588726853	27.69855243490462	30.17004402789475	24.556432948473773
20	19.92234987897597	28.359588511037494	34.08728408419581	17.630777525790727
21	20.23292129695903	31.419087571112065	30.333976905519872	18.014014226409035
22	17.820579244034	23.876140572442566	32.436256197440294	25.867023986083144
23	18.12395679903617	29.352704928813456	29.933009875938566	22.590328396211813
24	20.820928764043476	25.261286190035698	27.71897137932513	26.1988136665957
25	16.203631374834416	34.349678419791694	25.179755742918953	24.26693446245494
26	16.614698878702026	27.696517806990833	29.088784622282226	26.59999869202491
27	19.885871907093076	28.381751422241248	27.43223417404643	24.300142496619248
28	14.098445471608583	27.07668294611215	33.726137629498616	25.098733952780645
29	16.85020705972287	25.916218382427207	29.13311044468973	28.10046411316019
30	16.687073500206733	29.010160786470884	29.84174228094869	24.46102343237369
31	18.796546655108042	26.03909537536342	27.082786829853518	28.081571139675027
32	17.695376962053462	31.48644828811494	23.183931090059172	27.63424365977243
33	16.326290371922717	28.882923876576566	23.584680123792577	31.20610562770814
34	17.305237059584805	27.508314724965537	25.600269733529142	29.586178481920516
35	14.586974166765371	26.972626261378473	23.492467880128444	34.94793169172771
36	15.426766838180955	33.038869386334426	26.294295847978415	25.24006792750621
37	14.333662991498889	24.746016670869142	29.849154139777482	31.07116619785448
38	15.255858093422848	27.865827915530968	28.517853496617068	28.36046049442912
39	17.824285173448395	27.449964503009433	26.149328609121092	28.57642171442108
40	16.76685998054024	27.78182684880462	27.212421694074802	28.238891476580342
41	16.258638993789297	28.61711427269682	23.398003012702617	31.726243720811265
42	17.59887746671385	24.358783379705894	28.46764178631611	29.574697367264147
43	19.651889697007572	28.715793726515486	25.24580848483439	26.386508091642547
44	17.895133824017766	23.73822186600086	28.306470189431128	30.060174120550247
45	17.67219673689282	22.122799967736615	26.98330805792586	33.221695237444706
46	23.198246150738317	25.252275694988928	25.852563594838728	25.696914559434024
47	13.617619296411135	24.959507271251507	34.98055840363094	26.44231502870642
48	16.18074181080431	26.333825761731994	27.69673580283874	29.788696624624954
49	17.2444162180191	21.368824995113258	34.52937966374867	26.857379123118967
50	19.415582197877736	22.303663856215753	28.523521388662616	29.757232557243896
51	16.58396146414731	21.475061638325997	25.360982957811263	36.57999393971543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	291.0
2	483.0
3	2724.5
4	4966.0
5	3355.0
6	1744.0
7	1909.0
8	2074.0
9	2443.5
10	2813.0
11	3129.5
12	3446.0
13	3749.5
14	4053.0
15	3869.5
16	3686.0
17	3833.5
18	3981.0
19	4010.5
20	4040.0
21	4048.0
22	4056.0
23	4475.0
24	4894.0
25	5674.5
26	7714.5
27	8974.0
28	10255.5
29	11537.0
30	14989.5
31	18442.0
32	22564.5
33	26687.0
34	30059.5
35	33432.0
36	37776.0
37	42120.0
38	47766.5
39	53413.0
40	59588.0
41	65763.0
42	74918.0
43	84073.0
44	97561.0
45	111049.0
46	191779.5
47	272510.0
48	236184.5
49	199859.0
50	179443.0
51	159027.0
52	124766.0
53	90505.0
54	73882.0
55	57259.0
56	46532.0
57	35805.0
58	30172.0
59	24539.0
60	19631.5
61	14724.0
62	11632.5
63	8541.0
64	7075.5
65	5610.0
66	3964.0
67	2318.0
68	1867.0
69	1416.0
70	1084.5
71	753.0
72	594.5
73	436.0
74	364.5
75	204.0
76	115.0
77	135.5
78	156.0
79	83.5
80	11.0
81	8.5
82	6.0
83	4.5
84	3.0
85	3.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1376173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.135183253519266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13363998506615	21.524885499178616
2	13.554210677932907	8.440256666284974
3	5.345123138626477	4.992641653012842
4	2.780690027746237	3.4630917434045068
5	1.6301156727339547	2.537697509750276
6	1.073223834212003	2.0049012450141177
7	0.7821616689519563	1.7046922827688344
8	0.5975822112695225	1.488466532553588
9	0.4700554148159602	1.3171735331643544
>10	4.3292478413279465	28.257036278252407
>50	0.20852003678190212	4.189381304536264
>100	0.08391213849629946	4.70439880625787
>500	0.0049360080165930425	1.1123759118521246
>1k	0.0037607680126423185	2.2371928700868056
>5k	0.0018803840063211592	4.236322658512101
>10k+	9.401920031605796E-4	7.789485505370282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	34845	2.5320217734252886	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	33938	2.4661143620751167	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	26634	1.9353671377072506	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	11022	0.8009167452057262	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	8628	0.6269560585769376	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	8235	0.5983986025012844	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT	7792	0.5662078822938686	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	7290	0.5297299104109731	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	7274	0.5285672658888091	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	6757	0.4909993147663848	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	6431	0.4673104326272932	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA	5480	0.39820574884117044	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3309	0.2404494202400425	No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	3124	0.22700634295252123	No Hit
TCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	2759	0.20048351479065493	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT	2228	0.16189824971133718	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	2012	0.14620254866212315	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	1896	0.13777337587643415	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG	1850	0.13443077287521266	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	1789	0.12999819063446238	No Hit
TGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	1615	0.11735443145592886	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	1596	0.11597379108585912	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	1517	0.11023323375767435	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT	1487	0.10805327527861686	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	1444	0.1049286681253011	No Hit
GCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	1438	0.1044926764294896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4533056527050016E-4	0.0	0.0	0.6508629365639349	0.0
2	1.4533056527050016E-4	0.0	0.0	2.6371684373984956	0.0
3	1.4533056527050016E-4	0.0	0.0	3.6863097880862363	0.0
4	1.4533056527050016E-4	0.0	0.0	5.3912553145571085	0.0
5	2.179958479057502E-4	0.0	0.0	9.594360592745243	0.0
6	2.179958479057502E-4	0.0	0.0	10.744288690448077	0.0
7	2.179958479057502E-4	0.0	0.0	12.181753311538593	0.0
8	2.179958479057502E-4	0.0	0.0	14.137612058948983	0.0
9	2.179958479057502E-4	0.0	0.0	14.787603012121295	0.0
10	2.179958479057502E-4	0.0	0.0	18.08827814526226	0.0
11	2.179958479057502E-4	0.0	0.0	20.676397516881963	0.0
12	2.179958479057502E-4	0.0	0.0	25.90996916812058	0.0
13	2.179958479057502E-4	0.0	0.0	26.85214722276923	0.0
14	2.179958479057502E-4	0.0	0.0	27.22186818081738	0.0
15	2.179958479057502E-4	0.0	0.0	27.985652966596497	0.0
16	2.179958479057502E-4	0.0	0.0	28.915623253762426	0.0
17	2.179958479057502E-4	0.0	0.0	30.18806501798829	0.0
18	2.179958479057502E-4	0.0	0.0	31.516677045691203	0.0
19	2.179958479057502E-4	0.0	0.0	33.280554116379264	0.0
20	2.906611305410003E-4	0.0	0.0	34.37700056606255	0.0
21	2.906611305410003E-4	0.0	0.0	35.81250322452192	0.0
22	2.906611305410003E-4	0.0	0.0	37.46374910712534	0.0
23	2.906611305410003E-4	0.0	0.0	39.01231894536515	0.0
24	2.906611305410003E-4	0.0	0.0	40.08856444647584	0.0
25	2.906611305410003E-4	0.0	0.0	40.99404653339369	0.0
26	2.906611305410003E-4	0.0	0.0	41.80397377364619	0.0
27	2.906611305410003E-4	0.0	0.0	42.63940652810366	0.0
28	2.906611305410003E-4	0.0	0.0	43.48682905419595	0.0
29	2.906611305410003E-4	0.0	0.0	44.37676077062986	0.0
30	2.906611305410003E-4	0.0	0.0	45.359631383554245	0.0
31	2.906611305410003E-4	0.0	0.0	46.24810979433545	0.0
32	2.906611305410003E-4	0.0	0.0	47.07235209526709	0.0
33	2.906611305410003E-4	0.0	0.0	47.87058022501532	0.0
34	2.906611305410003E-4	0.0	0.0	48.66888102004617	0.0
35	2.906611305410003E-4	0.0	0.0	49.51012699711446	0.0
36	2.906611305410003E-4	0.0	0.0	50.27224048139296	0.0
37	2.906611305410003E-4	0.0	0.0	51.05121231124284	0.0
38	2.906611305410003E-4	0.0	0.0	51.82364426565555	0.0
39	2.906611305410003E-4	0.0	0.0	52.66343693707114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACCG	30	2.165816E-6	45.000004	1
TGCCGCG	30	2.165816E-6	45.000004	1
CGGGTTC	45	3.8562575E-10	45.0	6
TCGGCCG	20	7.033772E-4	45.0	1
TATTGCG	40	6.8175723E-9	45.0	1
TACGATT	20	7.033772E-4	45.0	42
CGTACAA	20	7.033772E-4	45.0	24
CCGCGCA	40	6.8175723E-9	45.0	40
CGCCGGA	20	7.033772E-4	45.0	39
ACGAGTA	20	7.033772E-4	45.0	14
TCGACTC	20	7.033772E-4	45.0	27
TCGCGGA	20	7.033772E-4	45.0	3
CACGGGT	25	3.8912094E-5	44.999996	4
ACGTTCC	25	3.8912094E-5	44.999996	10
ATGTCGG	25	3.8912094E-5	44.999996	2
GCGATCG	25	3.8912094E-5	44.999996	9
TTACGCG	25	3.8912094E-5	44.999996	1
CGTTTTT	2020	0.0	43.217823	1
TCGGGCG	200	0.0	42.749996	5
CTTTGCG	235	0.0	42.12766	1
>>END_MODULE