##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933907.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806159
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.434435390537104	33.0	31.0	34.0	31.0	34.0
2	32.65935007858251	34.0	31.0	34.0	31.0	34.0
3	32.71832231606916	34.0	31.0	34.0	31.0	34.0
4	36.08296378257887	37.0	35.0	37.0	35.0	37.0
5	36.11507159257665	37.0	35.0	37.0	35.0	37.0
6	35.7301314008775	37.0	35.0	37.0	35.0	37.0
7	36.060388831483614	37.0	35.0	37.0	35.0	37.0
8	36.05448180817928	37.0	36.0	37.0	35.0	37.0
9	37.885221153643386	39.0	38.0	39.0	35.0	39.0
10	37.588699003546445	39.0	37.0	39.0	35.0	39.0
11	37.5573615130514	39.0	37.0	39.0	35.0	39.0
12	37.36616597966406	39.0	37.0	39.0	35.0	39.0
13	37.27608821584824	39.0	37.0	39.0	34.0	39.0
14	38.528507403626335	40.0	38.0	41.0	34.0	41.0
15	38.58526791861159	40.0	38.0	41.0	35.0	41.0
16	38.64770225228522	40.0	38.0	41.0	35.0	41.0
17	38.63866557341666	40.0	38.0	41.0	35.0	41.0
18	38.588555111336596	40.0	38.0	41.0	35.0	41.0
19	38.60844076664777	40.0	38.0	41.0	35.0	41.0
20	38.553849798861016	40.0	38.0	41.0	34.0	41.0
21	38.47279134761257	40.0	38.0	41.0	34.0	41.0
22	38.46854404652184	40.0	38.0	41.0	34.0	41.0
23	38.40313635399468	40.0	37.0	41.0	34.0	41.0
24	38.372684544860256	40.0	37.0	41.0	34.0	41.0
25	38.29326720907414	40.0	37.0	41.0	34.0	41.0
26	38.21506303347106	40.0	37.0	41.0	34.0	41.0
27	38.21023272084043	40.0	37.0	41.0	34.0	41.0
28	38.14678990124777	40.0	37.0	41.0	34.0	41.0
29	38.137915721340335	40.0	37.0	41.0	34.0	41.0
30	38.09810843766552	40.0	37.0	41.0	34.0	41.0
31	38.03470035067524	40.0	37.0	41.0	34.0	41.0
32	37.96900735462855	40.0	37.0	41.0	34.0	41.0
33	37.920901459885705	40.0	37.0	41.0	34.0	41.0
34	37.89696449459722	40.0	37.0	41.0	34.0	41.0
35	37.83722938030835	40.0	37.0	41.0	33.0	41.0
36	37.74711564344007	40.0	36.0	41.0	33.0	41.0
37	37.69945755117787	40.0	36.0	41.0	33.0	41.0
38	37.66791786731898	40.0	36.0	41.0	33.0	41.0
39	37.63626902385261	40.0	36.0	41.0	33.0	41.0
40	37.51132592950026	40.0	36.0	41.0	33.0	41.0
41	37.38178696758332	40.0	35.0	41.0	33.0	41.0
42	37.35225185106164	40.0	35.0	41.0	33.0	41.0
43	37.262069889438685	39.0	35.0	41.0	33.0	41.0
44	37.16407557318097	39.0	35.0	41.0	32.0	41.0
45	37.16444150595602	39.0	35.0	41.0	32.0	41.0
46	37.08458753174002	39.0	35.0	41.0	32.0	41.0
47	37.01509751798343	39.0	35.0	41.0	32.0	41.0
48	36.9724148710118	39.0	35.0	41.0	32.0	41.0
49	36.951874258055796	39.0	35.0	41.0	32.0	41.0
50	36.88335675716577	39.0	35.0	41.0	32.0	41.0
51	35.98665027618621	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	17.0
11	17.0
12	16.0
13	9.0
14	9.0
15	19.0
16	23.0
17	63.0
18	113.0
19	180.0
20	332.0
21	551.0
22	893.0
23	1288.0
24	1841.0
25	2749.0
26	3734.0
27	4763.0
28	5699.0
29	7166.0
30	9037.0
31	11513.0
32	15297.0
33	22223.0
34	43700.0
35	61299.0
36	54964.0
37	85586.0
38	169050.0
39	303806.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.936395921896302	4.906972445882264	54.16561745263652	15.99101417958492
2	20.45489785513776	4.743481124691283	52.35977021902627	22.441850801144685
3	21.48012985031489	4.699693236694002	51.05394841464277	22.76622849834834
4	20.04666573219427	5.49928736142622	49.39608687616215	25.057960030217362
5	18.979630569155713	6.321085542678305	49.08088850958682	25.618395378579162
6	21.84060464498939	6.919974843672278	52.063426693741555	19.175993817596776
7	83.6755528375916	2.6231797945566573	8.767625245143948	4.933642122707804
8	84.07547394496619	3.6543659501413495	7.124897197699213	5.145262907193246
9	77.91825185850433	6.2462864025583045	10.027798486402805	5.8076632525345495
10	37.651629517254044	30.75100569490634	16.908451062383474	14.688913725456146
11	30.962254344366308	25.519159371786458	24.2140818374539	19.30450444639333
12	30.071115003367822	21.8254711539535	28.267748669927396	19.835665172751284
13	25.355668050595476	23.995266442475987	29.23008984579965	21.418975661128883
14	20.321673516018553	26.237504015957153	29.69017278229233	23.750649685731968
15	17.75257734516392	25.9307407099592	34.98156070948783	21.33512123538905
16	20.72134653337617	24.221648582971845	33.06767027348203	21.98933461016995
17	20.599658380046616	24.58237146766333	28.884624497152544	25.933345655137508
18	21.524910098380097	25.356164230629442	30.119492556679266	22.999433114311195
19	24.819669568906384	26.29245595471861	27.364701008113784	21.523173468261227
20	27.201085641914315	25.703118119378438	27.559327626435977	19.53646861227128
21	25.964108817243247	26.368991724957485	27.05272781175922	20.61417164604005
22	23.84355939709164	25.386803347726687	26.872490414421968	23.8971468407597
23	23.43644367922457	26.143105764495587	26.96291922561182	23.45753133066802
24	23.898883470878573	23.20410737832115	28.46994203376753	24.427067117032745
25	21.853009145838477	25.618271333570675	26.865667938954967	25.66305158163588
26	21.404338350126963	27.159282474052887	27.032632520383697	24.40374665543646
27	21.525034143388588	25.926523179670514	29.539706186992888	23.008736489948014
28	19.436612380436117	25.980234668347062	30.496713427500033	24.086439523716784
29	20.352808813149764	24.422849586744057	29.786927888915216	25.43741371119097
30	21.688153329554094	23.85025782755015	30.25073217566262	24.210856667233138
31	21.156124288136706	26.10055832658322	28.53171644799599	24.211600937284082
32	23.60266399060235	25.44795753691269	27.15618134884061	23.793197123644344
33	22.93927128519312	24.653077122503127	28.253235403933964	24.154416188369787
34	21.214673532144403	23.75461912600368	29.949302805029777	25.081404536822138
35	21.08479840825445	22.57606750033182	28.56657309538193	27.772560996031796
36	20.723083163495044	24.882312298194275	29.221654785222267	25.172949753088407
37	20.693808541491197	25.99933759965466	30.576226277942688	22.73062758091146
38	21.145332372398002	24.728620532674075	28.922210134725283	25.20383696020264
39	21.15934945835747	23.327904296795047	29.542311132171196	25.97043511267628
40	23.2564543719043	22.19586954930727	31.006290322380575	23.541385756407855
41	19.868661145009856	23.39985040171976	29.986516307577045	26.744972145693342
42	21.97196830898123	23.673865825476113	28.021147193047526	26.33301867249513
43	21.90684467952352	23.117275872377533	28.612593793532042	26.363285654566905
44	21.099807854281845	23.675478410586496	28.6553893214614	26.56932441367026
45	21.51994829804046	21.918504910321662	28.29193744658312	28.269609345054757
46	21.17894856969903	23.453934025421784	29.85825376879747	25.508863636081713
47	19.315172317123544	23.192323102514518	31.952133512123538	25.5403710682384
48	19.958593776165745	21.591150132914226	31.73517879227299	26.71507729864704
49	20.32874408150253	21.730948857483448	32.022342986929374	25.917964074084644
50	19.760121762580336	21.723134021948525	31.032091684146675	27.484652531324468
51	18.802742387047715	21.743353358332538	28.852993019987373	30.60091123463237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	409.0
2	721.0
3	1359.5
4	1998.0
5	1444.0
6	890.0
7	915.5
8	941.0
9	971.5
10	1002.0
11	1039.5
12	1077.0
13	1131.0
14	1185.0
15	1130.0
16	1075.0
17	1226.5
18	1378.0
19	1447.5
20	1517.0
21	1799.0
22	2081.0
23	2375.0
24	2669.0
25	3746.0
26	5874.5
27	6926.0
28	8165.0
29	9404.0
30	10476.0
31	11548.0
32	13776.0
33	16004.0
34	18148.0
35	20292.0
36	21376.0
37	22460.0
38	26334.0
39	30208.0
40	33671.0
41	37134.0
42	42454.5
43	47775.0
44	51865.5
45	55956.0
46	63103.0
47	70250.0
48	78345.5
49	86441.0
50	84500.0
51	82559.0
52	70873.5
53	59188.0
54	53027.0
55	46866.0
56	43965.5
57	41065.0
58	39952.0
59	38839.0
60	36488.5
61	34138.0
62	30126.0
63	26114.0
64	20663.0
65	15212.0
66	12634.0
67	10056.0
68	8389.0
69	6722.0
70	5332.0
71	3942.0
72	3312.5
73	2683.0
74	2033.0
75	1133.5
76	884.0
77	586.5
78	289.0
79	276.0
80	263.0
81	158.0
82	53.0
83	46.0
84	39.0
85	22.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	806159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.18151324975238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.44178876961693	19.56974690297466
2	12.549679613257075	7.073379246023031
3	4.954908933973553	4.189104953222764
4	2.4756628902470226	2.79071706173667
5	1.3906594675518653	1.959544410535324
6	0.8886881927187996	1.5026746846802008
7	0.6667354967763903	1.3152730665538857
8	0.4991806220850116	1.1254132252246716
9	0.41814162608240113	1.0605477398142775
>10	6.033306613155776	43.62909260943277
>50	0.6033272775001967	10.189057297813614
>100	0.07302263722132274	3.4543664798602913
>500	0.003116819881397922	0.6472310037821709
>1k	0.001781039932227384	1.4938513183456272
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3325	0.41244965323217875	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	3135	0.38888110161891143	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2888	0.35824198452166384	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2557	0.3171830867111823	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	956	0.11858702811728208	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	940	0.11660230798142797	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	817	0.10134477193704963	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.721350254726425E-4	0.0	0.0	0.4050069527227259	0.0
2	3.721350254726425E-4	0.0	0.0	1.5778525080040042	0.0
3	3.721350254726425E-4	0.0	0.0	2.2517890391349598	0.0
4	3.721350254726425E-4	0.0	0.0	2.9505345719640914	0.0
5	3.721350254726425E-4	0.0	0.0	4.074382348891472	0.0
6	3.721350254726425E-4	0.0	0.0	4.8289481355415	0.0
7	3.721350254726425E-4	0.0	0.0	5.491844660916767	0.0
8	3.721350254726425E-4	0.0	0.0	6.595473101460134	0.0
9	3.721350254726425E-4	0.0	0.0	7.125021242707704	0.0
10	3.721350254726425E-4	0.0	0.0	8.11167524024417	0.0
11	3.721350254726425E-4	0.0	0.0	9.881301331375075	0.0
12	3.721350254726425E-4	0.0	0.0	11.460766424489462	0.0
13	3.721350254726425E-4	0.0	0.0	11.996764906178559	0.0
14	3.721350254726425E-4	0.0	0.0	12.18580949911866	0.0
15	3.721350254726425E-4	0.0	0.0	12.492200670091135	0.0
16	3.721350254726425E-4	0.0	0.0	13.207940369083518	0.0
17	3.721350254726425E-4	0.0	0.0	14.167924689794445	0.0
18	3.721350254726425E-4	0.0	0.0	15.17256521356209	0.0
19	3.721350254726425E-4	0.0	0.0	15.822188923028833	0.0
20	3.721350254726425E-4	0.0	0.0	16.57997987989962	0.0
21	3.721350254726425E-4	0.0	0.0	17.58102309842103	0.0
22	3.721350254726425E-4	0.0	0.0	18.667409282784167	0.0
23	3.721350254726425E-4	0.0	0.0	19.6735383466537	0.0
24	3.721350254726425E-4	0.0	0.0	20.435050653779218	0.0
25	3.721350254726425E-4	0.0	0.0	21.16331889862918	0.0
26	3.721350254726425E-4	0.0	0.0	21.761587974580696	0.0
27	3.721350254726425E-4	0.0	0.0	22.36221390569354	0.0
28	3.721350254726425E-4	0.0	0.0	22.99025378368287	0.0
29	3.721350254726425E-4	0.0	0.0	23.648436598735486	0.0
30	3.721350254726425E-4	0.0	0.0	24.365168657795795	0.0
31	3.721350254726425E-4	0.0	0.0	25.058208120234347	0.0
32	3.721350254726425E-4	0.0	0.0	25.72631453596623	0.0
33	3.721350254726425E-4	0.0	0.0	26.357827674193306	0.0
34	4.961800339635233E-4	0.0	0.0	26.994674747785485	0.0
35	4.961800339635233E-4	0.0	0.0	27.64293396215883	0.0
36	4.961800339635233E-4	0.0	0.0	28.324313193799238	0.0
37	4.961800339635233E-4	0.0	0.0	28.99366005961603	0.0
38	4.961800339635233E-4	0.0	0.0	29.633608258420484	0.0
39	4.961800339635233E-4	0.0	0.0	30.31610389513731	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	20	7.0320326E-4	45.0	26
GGCGTAC	20	7.0320326E-4	45.0	16
CGGCACT	20	7.0320326E-4	45.0	17
ATTACCG	25	3.8897662E-5	45.0	20
CCGCTAT	40	6.8102963E-9	45.0	25
TATTGCG	40	6.8102963E-9	45.0	1
GCCGATA	20	7.0320326E-4	45.0	9
CCCCGAC	20	7.0320326E-4	45.0	41
TACGAGA	20	7.0320326E-4	45.0	35
CACTACG	25	3.8897662E-5	45.0	25
GTCCGTT	20	7.0320326E-4	45.0	23
CATACGT	25	3.8897662E-5	45.0	39
TCGTAAT	20	7.0320326E-4	45.0	22
ACTCCGC	20	7.0320326E-4	45.0	30
TAGTGCG	20	7.0320326E-4	45.0	16
TGGTCGA	20	7.0320326E-4	45.0	17
CCCGCTA	40	6.8102963E-9	45.0	24
GTACGTC	20	7.0320326E-4	45.0	37
GTACGGA	20	7.0320326E-4	45.0	37
TGCCGTA	20	7.0320326E-4	45.0	39
>>END_MODULE