##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	707261
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.323025021880184	33.0	31.0	34.0	31.0	34.0
2	32.525076315532736	34.0	31.0	34.0	31.0	34.0
3	32.61339166163552	34.0	31.0	34.0	31.0	34.0
4	36.02627460018296	37.0	35.0	37.0	35.0	37.0
5	36.038741002260835	37.0	35.0	37.0	35.0	37.0
6	35.49171380862228	37.0	35.0	37.0	33.0	37.0
7	35.924573813627504	37.0	35.0	37.0	35.0	37.0
8	35.863577943644565	37.0	35.0	37.0	35.0	37.0
9	37.77119903401997	39.0	38.0	39.0	35.0	39.0
10	37.49151868970578	39.0	37.0	39.0	35.0	39.0
11	37.47539734270658	39.0	37.0	39.0	35.0	39.0
12	37.218823037040075	39.0	37.0	39.0	34.0	39.0
13	37.064079597206685	39.0	37.0	39.0	33.0	39.0
14	38.12133568795678	40.0	37.0	41.0	33.0	41.0
15	38.187712881100474	40.0	37.0	41.0	33.0	41.0
16	38.38489185746139	40.0	37.0	41.0	34.0	41.0
17	38.31153704219517	40.0	37.0	41.0	34.0	41.0
18	38.30177119903402	40.0	37.0	41.0	34.0	41.0
19	38.321988629374445	40.0	37.0	41.0	34.0	41.0
20	38.219183582864034	40.0	37.0	41.0	34.0	41.0
21	38.184537250039234	40.0	37.0	41.0	34.0	41.0
22	38.22277631595691	40.0	37.0	41.0	34.0	41.0
23	38.15224365545393	40.0	37.0	41.0	34.0	41.0
24	38.098236718835054	40.0	37.0	41.0	34.0	41.0
25	38.0254729159391	40.0	36.0	41.0	34.0	41.0
26	37.98870572532629	40.0	36.0	41.0	34.0	41.0
27	37.96439221164464	40.0	36.0	41.0	34.0	41.0
28	37.90754615340023	40.0	36.0	41.0	34.0	41.0
29	37.92616021525293	40.0	36.0	41.0	34.0	41.0
30	37.86830604260662	40.0	36.0	41.0	34.0	41.0
31	37.8006252288759	40.0	36.0	41.0	33.0	41.0
32	37.65764972195554	40.0	36.0	41.0	33.0	41.0
33	37.59466024565189	40.0	36.0	41.0	33.0	41.0
34	37.50768669557632	40.0	36.0	41.0	33.0	41.0
35	37.529342067497005	40.0	36.0	41.0	33.0	41.0
36	37.44866039552584	40.0	36.0	41.0	33.0	41.0
37	37.38585189908676	40.0	35.0	41.0	33.0	41.0
38	37.34827312689375	40.0	35.0	41.0	33.0	41.0
39	37.266447605622254	39.0	35.0	41.0	33.0	41.0
40	37.13534041888355	39.0	35.0	41.0	32.0	41.0
41	36.927376173718045	39.0	35.0	41.0	31.0	41.0
42	37.04167061381866	39.0	35.0	41.0	32.0	41.0
43	36.95469282202751	39.0	35.0	41.0	32.0	41.0
44	36.88561082825152	39.0	35.0	41.0	32.0	41.0
45	36.90386858599584	39.0	35.0	41.0	32.0	41.0
46	36.808185379937534	39.0	35.0	41.0	32.0	41.0
47	36.67872963446309	39.0	35.0	41.0	31.0	41.0
48	36.64970216087131	39.0	35.0	41.0	31.0	41.0
49	36.70386038534572	39.0	35.0	41.0	32.0	41.0
50	36.60662895310218	39.0	35.0	40.0	31.0	41.0
51	35.67608704565924	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	15.0
11	11.0
12	13.0
13	9.0
14	8.0
15	9.0
16	28.0
17	60.0
18	90.0
19	184.0
20	396.0
21	760.0
22	1179.0
23	1707.0
24	2302.0
25	2889.0
26	3859.0
27	4531.0
28	5375.0
29	6780.0
30	8771.0
31	11565.0
32	16153.0
33	23259.0
34	45516.0
35	54697.0
36	50275.0
37	79579.0
38	151469.0
39	235617.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.5278235333208	4.3928620410287005	49.27728236110856	14.802032064541946
2	18.88199688658077	11.055183305738616	47.72382472665678	22.33899508102384
3	20.387098963466105	11.524175657925435	46.786546974879144	21.302178403729314
4	18.85117375339514	4.7237158559569945	52.43665351263537	23.988456878012503
5	23.13898263865815	5.864030393306007	46.234134216364254	24.76285275167159
6	21.06874265652991	11.203926131937148	49.2146463611029	18.512684850430038
7	77.9273846571492	2.510247277879029	14.496204371512073	5.066163693459699
8	77.51480712212323	7.598185111295547	8.742741364220564	6.144266402360656
9	71.89538232703345	5.442403865051233	10.891453084504873	11.770760723410453
10	38.81664618860647	26.343315975290594	19.17722029067063	15.66281754543231
11	32.694578097760235	22.57752088691445	24.344336814839217	20.383564200486102
12	26.864764210100656	20.560443740005457	29.53817614713663	23.03661590275726
13	24.87285457560929	23.237531830540632	33.11761853120701	18.771995062643068
14	20.21389557744595	27.490417257561212	30.711717456497674	21.583969708495164
15	16.319717897636092	23.36732832716635	38.08848501472582	22.224468760471737
16	20.347085446532468	25.020324887135022	28.944618747534502	25.68797091879801
17	20.892570069606553	24.56137126181141	29.85404256702971	24.692016101552326
18	22.4088419975087	22.7459169952818	31.153138657440465	23.69210234976904
19	21.574496543708758	25.12269162303591	29.023797438286575	24.27901439496876
20	24.261057799030343	26.37272520328422	30.298291578356505	19.067925419328933
21	22.68243265216094	29.197566386383528	29.23461070241396	18.88539025904157
22	21.34134357754775	23.006923893725233	30.635225185610405	25.01650734311662
23	20.881824390147344	27.684546440422984	28.68163238182227	22.751996787607403
24	22.22545849410614	23.825574999893956	28.696902557895882	25.252063948104027
25	19.950909211733716	29.65044021938153	25.781430051989297	24.61722051689546
26	19.519526737654132	24.962778945820567	29.280704011673205	26.2369903048521
27	21.021518223117067	26.075380941406358	27.935231830964806	24.967869004511773
28	15.6540513332419	25.129619758476718	31.958499054804378	27.257829853477006
29	19.420836155252445	23.05287581246527	31.379504878679864	26.14678315360242
30	21.69015398841446	24.60308146497545	29.43580941123574	24.270955135374354
31	22.594911920776063	24.814036119622035	26.613937429039638	25.977114530562268
32	23.002823568668425	25.377477338634534	27.90638816504798	23.713310927649054
33	22.57836923002965	23.093454891475705	26.26130947415452	28.066866404340125
34	19.610440841499813	24.25794720760794	30.824264309780975	25.30734764111127
35	18.438313437330773	23.177865031438184	29.500000706952594	28.88382082427845
36	21.19655968588682	23.719814891532263	29.729194738576	25.354430684004914
37	18.191021419249754	24.39170263877126	29.907912354844957	27.50936358713403
38	20.685291568459167	24.746875623001976	26.985653104016766	27.58217970452209
39	21.670217925207243	24.36978710829524	30.319924327794123	23.640070638703392
40	24.214398927694305	22.47840613295516	28.733098530811112	24.574096408539422
41	18.363093681116307	25.763190675012478	27.69189874742139	28.181816896449824
42	19.53960419138055	21.67983248051285	30.754841564853706	28.025721763252886
43	21.928962575343473	20.546304688085446	30.6301351269192	26.894597609651882
44	20.93173524342499	22.293325943322195	29.43496106812054	27.339977745132277
45	20.701975649724783	20.843931731001707	27.58260387607969	30.871488743193815
46	23.10886645806852	22.89296313524993	28.54830112221655	25.449869284465
47	16.87778627691899	22.733474629592187	34.89588709118699	25.492852002301834
48	19.351130629286782	24.270530963816753	29.959802675391405	26.41853573150506
49	20.90034654816256	20.174306232069913	33.91845443195652	25.006892787811008
50	21.339646891317347	21.479199333767873	29.860970702470517	27.32018307244426
51	19.107656155224166	20.93512861588579	27.23888352390419	32.71833170498585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	278.5
2	469.0
3	1154.0
4	1839.0
5	1299.0
6	759.0
7	794.5
8	830.0
9	918.0
10	1006.0
11	982.0
12	958.0
13	998.5
14	1039.0
15	993.5
16	948.0
17	917.5
18	887.0
19	956.0
20	1025.0
21	1172.5
22	1320.0
23	1679.5
24	2039.0
25	2636.5
26	3676.0
27	4118.0
28	5456.5
29	6795.0
30	7918.5
31	9042.0
32	11027.0
33	13012.0
34	14734.0
35	16456.0
36	17796.5
37	19137.0
38	21138.5
39	23140.0
40	26033.0
41	28926.0
42	33111.0
43	37296.0
44	42299.5
45	47303.0
46	71397.5
47	95492.0
48	89968.0
49	84444.0
50	82444.0
51	80444.0
52	68364.5
53	56285.0
54	50176.5
55	44068.0
56	38197.0
57	32326.0
58	29332.5
59	26339.0
60	23916.5
61	21494.0
62	18570.0
63	15646.0
64	12975.5
65	10305.0
66	8747.5
67	7190.0
68	5970.0
69	4750.0
70	3712.5
71	2675.0
72	2241.5
73	1808.0
74	1417.0
75	897.0
76	768.0
77	549.0
78	330.0
79	238.0
80	146.0
81	96.0
82	46.0
83	24.5
84	3.0
85	4.0
86	5.0
87	5.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	707261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.26147069250826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.65561748943851	19.403087215538246
2	12.751528735669984	7.207539112956283
3	5.277302808758966	4.474330159956992
4	2.8090013680967316	3.175460393587256
5	1.5115786827054363	2.1359718320349965
6	1.0265109976169404	1.7406426284813148
7	0.7160698638108366	1.4166031228914837
8	0.5836461936243537	1.3195759836726941
9	0.44739290907339063	1.1379583429032278
>10	5.792160669664355	40.0047729431828
>50	0.3507415862121264	6.043890860758924
>100	0.06680792118286272	3.49382491986892
>500	0.005061206150216872	0.996968791103184
>1k	0.005061206150216872	3.541313246790908
>5k	0.0010122412300433744	2.424622378477772
>10k+	5.061206150216872E-4	1.4834380677949959
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	10371	1.4663610746245022	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	8605	1.2166654177170804	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	8346	1.180045273244248	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	4784	0.6764122438534007	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3359	0.4749307539932217	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	3176	0.44905628897959876	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2665	0.3768057336683346	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT	2043	0.2888608307258565	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTGACTT	2021	0.2857502393034537	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	1947	0.27528734088264445	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTC	1908	0.2697731106338396	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTA	1755	0.24814036119622038	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	1100	0.15552957112013813	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	968	0.13686602258572153	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	961	0.13587628895132065	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	838	0.1184852550897052	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	733	0.10363925057369204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46673010387961444	0.0
2	0.0	0.0	0.0	2.0955488850650608	0.0
3	0.0	0.0	0.0	2.9127860860417867	0.0
4	0.0	0.0	0.0	4.016197697879567	0.0
5	0.0	0.0	0.0	6.5895051473218516	0.0
6	0.0	0.0	0.0	7.433747937465801	0.0
7	0.0	0.0	0.0	8.492055973678742	0.0
8	0.0	0.0	0.0	9.97877728306806	0.0
9	0.0	0.0	0.0	10.533593680409354	0.0
10	0.0	0.0	0.0	12.866537247211426	0.0
11	0.0	0.0	0.0	14.832855197727572	0.0
12	0.0	0.0	0.0	18.27797658855783	0.0
13	0.0	0.0	0.0	19.01886290916649	0.0
14	0.0	0.0	0.0	19.32525616427316	0.0
15	0.0	0.0	0.0	19.95288867900252	0.0
16	0.0	0.0	0.0	20.819895342737688	0.0
17	0.0	0.0	0.0	21.874527225451423	0.0
18	0.0	0.0	0.0	22.987553392594812	0.0
19	0.0	0.0	0.0	24.10892160037101	0.0
20	0.0	0.0	0.0	25.005478882619006	0.0
21	0.0	0.0	0.0	26.198673474148865	0.0
22	0.0	0.0	0.0	27.414349158231545	0.0
23	0.0	0.0	0.0	28.4918863050557	0.0
24	0.0	0.0	0.0	29.362851903328473	0.0
25	0.0	0.0	0.0	30.130885203623556	0.0
26	0.0	0.0	0.0	30.83416164612498	0.0
27	0.0	0.0	0.0	31.54281092835601	0.0
28	0.0	0.0	0.0	32.24340095099263	0.0
29	0.0	0.0	0.0	32.993760436387696	0.0
30	0.0	0.0	0.0	33.91972694662932	0.0
31	0.0	0.0	0.0	34.642798061818766	0.0
32	0.0	0.0	0.0	35.34225696030178	0.0
33	0.0	0.0	0.0	36.057410206416016	0.0
34	0.0	0.0	0.0	36.77256345253026	0.0
35	1.4139051920012555E-4	0.0	0.0	37.557846396167754	0.0
36	1.4139051920012555E-4	0.0	0.0	38.195659028279515	0.0
37	1.4139051920012555E-4	0.0	0.0	38.88748283872573	0.0
38	1.4139051920012555E-4	0.0	0.0	39.6347317326984	0.0
39	1.4139051920012555E-4	0.0	0.0	40.68738414814333	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTG	20	7.0314447E-4	45.0	18
TATTGCG	40	6.8084773E-9	45.0	1
AACGGTC	20	7.0314447E-4	45.0	13
ATCCGGT	20	7.0314447E-4	45.0	29
AACGCGC	20	7.0314447E-4	45.0	32
GATGCGA	20	7.0314447E-4	45.0	16
CAATCGG	25	3.8892787E-5	45.0	20
TGCGATA	30	2.1643136E-6	44.999996	11
ACGCGCA	30	2.1643136E-6	44.999996	15
TTCTTCG	135	0.0	41.666668	1
CGACCAA	265	0.0	41.603775	29
TGTTTCG	115	0.0	41.08696	1
CGGGATA	115	0.0	41.08696	6
ACACGAC	275	0.0	40.909092	26
TACGGCT	1435	0.0	40.296165	7
CACGACC	280	0.0	40.17857	27
CTACGGC	140	0.0	40.17857	6
ACGACCA	275	0.0	40.090908	28
TTATCCG	45	1.9264917E-8	40.0	1
ACGTGAG	225	0.0	40.0	44
>>END_MODULE