FastQCFastQC Report
Sat 14 Jan 2017
SRR2933904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250908
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC20440.8146412230777815TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT20260.8074672788432413TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC16090.6412709040763945TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC13690.5456183142825258TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG7610.3032984201380586No Hit
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC6220.24789962854910963No Hit
TCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG5910.23554450236740163No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC5700.22717490076043811No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG5570.2219937188132702No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC5390.21481977457873005No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.21322556474883225No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC4480.17855150094855485No Hit
GCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG4190.16699347968179573No Hit
CGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT3860.15384124858513878No Hit
TTCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT3800.15144993384029207No Hit
TGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG3790.1510513813828176No Hit
CGTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT2890.11518166021011686No Hit
TTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG2840.11318889792274459No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC2560.10202942911345993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG351.2068631E-745.0000043
TTGCGCG351.2068631E-745.0000041
TCGCCAC207.0227374E-445.042
CGTGGCA253.8820624E-545.015
GCACGTG253.8820624E-545.012
CGCGACC253.8820624E-545.010
GCGTTAG207.0227374E-445.01
TCTACGG207.0227374E-445.02
GACGGGA207.0227374E-445.04
AATTCCG207.0227374E-445.011
TTGTGCG600.044.9999961
CGTGCGG650.044.9999962
GGCACGT302.1586984E-644.99999611
TTCGGGT302.1586984E-644.9999964
TGTGTCG302.1586984E-644.9999961
CGGGAAT700.041.7857176
CGTTTTT4100.041.158541
TATGGGA556.002665E-1140.9090924
TCGGGAT950.040.2631575
TTTGGGC4850.039.8969084
Produced by FastQC (version 0.11.5)