##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933904.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250908
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33336920305451	33.0	31.0	34.0	31.0	34.0
2	32.560161493455766	34.0	31.0	34.0	31.0	34.0
3	32.61760485915156	34.0	31.0	34.0	31.0	34.0
4	36.011880848757315	37.0	35.0	37.0	35.0	37.0
5	36.03422369952333	37.0	35.0	37.0	35.0	37.0
6	35.63153028201572	37.0	35.0	37.0	35.0	37.0
7	35.91635579574984	37.0	35.0	37.0	35.0	37.0
8	35.89790281696877	37.0	35.0	37.0	35.0	37.0
9	37.72179444258453	39.0	38.0	39.0	35.0	39.0
10	37.50491415180066	39.0	37.0	39.0	35.0	39.0
11	37.486154287627336	39.0	37.0	39.0	35.0	39.0
12	37.33941922935897	39.0	37.0	39.0	35.0	39.0
13	37.26208012498605	39.0	37.0	39.0	34.0	39.0
14	38.367549061807516	40.0	38.0	41.0	34.0	41.0
15	38.44796897667671	40.0	38.0	41.0	34.0	41.0
16	38.53111897587961	40.0	38.0	41.0	34.0	41.0
17	38.51142251343122	40.0	38.0	41.0	34.0	41.0
18	38.470885742981494	40.0	38.0	41.0	34.0	41.0
19	38.50876815406444	40.0	38.0	41.0	34.0	41.0
20	38.431604412772806	40.0	38.0	41.0	34.0	41.0
21	38.356820029652305	40.0	38.0	41.0	34.0	41.0
22	38.3639062923462	40.0	37.0	41.0	34.0	41.0
23	38.31231367672613	40.0	37.0	41.0	34.0	41.0
24	38.248158687646466	40.0	37.0	41.0	34.0	41.0
25	38.143008592790984	40.0	37.0	41.0	34.0	41.0
26	38.054565816952824	40.0	37.0	41.0	34.0	41.0
27	38.014367816091955	40.0	37.0	41.0	34.0	41.0
28	37.93702871171904	40.0	37.0	41.0	34.0	41.0
29	37.89870789293287	40.0	37.0	41.0	34.0	41.0
30	37.78679436287404	40.0	36.0	41.0	33.0	41.0
31	37.74408946705565	40.0	36.0	41.0	33.0	41.0
32	37.67219458925184	40.0	36.0	41.0	33.0	41.0
33	37.596358027643596	40.0	36.0	41.0	33.0	41.0
34	37.562895563314044	40.0	36.0	41.0	33.0	41.0
35	37.52924577932948	40.0	36.0	41.0	33.0	41.0
36	37.422672852200805	40.0	36.0	41.0	33.0	41.0
37	37.33747827889107	40.0	35.0	41.0	33.0	41.0
38	37.27330734771311	40.0	35.0	41.0	32.0	41.0
39	37.22906802493344	39.0	35.0	41.0	32.0	41.0
40	37.08474022350822	39.0	35.0	41.0	32.0	41.0
41	36.96176686275447	39.0	35.0	41.0	31.0	41.0
42	36.97726258230108	39.0	35.0	41.0	32.0	41.0
43	36.890003507261625	39.0	35.0	41.0	31.0	41.0
44	36.764949702679864	39.0	35.0	41.0	31.0	41.0
45	36.7628931719993	39.0	35.0	41.0	31.0	41.0
46	36.66741195976214	39.0	35.0	41.0	31.0	41.0
47	36.58963046216143	39.0	35.0	41.0	31.0	41.0
48	36.566590144594834	39.0	35.0	41.0	31.0	41.0
49	36.56464520860236	39.0	35.0	41.0	31.0	41.0
50	36.50200073333652	39.0	35.0	40.0	31.0	41.0
51	35.57400720582843	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	11.0
11	15.0
12	6.0
13	5.0
14	6.0
15	10.0
16	14.0
17	31.0
18	52.0
19	95.0
20	165.0
21	219.0
22	402.0
23	571.0
24	762.0
25	1051.0
26	1447.0
27	1807.0
28	2107.0
29	2404.0
30	3084.0
31	4027.0
32	5425.0
33	7968.0
34	14540.0
35	16852.0
36	19287.0
37	29911.0
38	55726.0
39	82850.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.20362842157285	4.717266886667623	52.14540787858498	16.93369681317455
2	21.987740526408086	5.903757552569069	48.75292936056243	23.35557256046041
3	22.131219411098886	5.894192293589683	48.66843623957786	23.306152055733577
4	20.280341798587532	5.148102093197507	48.452022255169226	26.11953385304574
5	19.68251311237585	6.317056450970076	46.969008560906786	27.031421875747284
6	21.880131362889983	7.369633491160106	51.06971479586143	19.68052035008848
7	77.4945398313326	3.08081049627752	13.01592615620068	6.408723516189201
8	76.64761585919939	4.743969901318412	11.06660608669313	7.54180815278907
9	69.88617341814529	6.4593396782884565	14.425207645830342	9.229279257735904
10	35.895228529979114	26.386962552011095	21.564477816570218	16.153331101439573
11	27.60015623256333	24.17818483268768	27.712946578028603	20.50871235672039
12	24.994021713137883	21.289476620912843	32.7331133323768	20.983388333572464
13	24.500215218327035	22.41299599853333	32.690866771884515	20.39592201125512
14	19.623128796212157	25.22996476796276	32.35966967972325	22.78723675610184
15	17.71167121016468	25.30529118242543	34.36797551293701	22.615062094472872
16	21.028026208809603	26.159389098793184	28.76193664610136	24.05064804629585
17	21.10972946259187	26.35547690787061	28.154941253367767	24.37985237616975
18	21.962631721587194	24.552027037798716	30.475313660784032	23.01002757983006
19	22.263538826980408	26.01192468952764	28.279289620099796	23.44524686339216
20	22.98452022255169	27.445517879063242	29.74556411114831	19.824397787236755
21	23.980104261322875	26.60776061345194	30.166435506241328	19.24569961898385
22	22.132813620928786	24.435649720216173	29.599295359255184	23.832241299599854
23	21.961037511757297	25.07971049149489	28.9516476158592	24.007604380888612
24	22.967781019337767	24.803513638465095	28.000701452325156	24.228003889871985
25	21.53259361997226	26.819790520828352	25.43482073110463	26.21279512809476
26	20.014108756994595	26.64243467725222	26.323194158815184	27.020262406938002
27	20.927192437068566	26.98359558085035	27.862802302038997	24.22640968004209
28	17.50123551261817	26.390150971670888	29.326685478342657	26.78192803736828
29	19.570121319368056	25.838952922983722	27.297654917340218	27.293270840308004
30	20.42182792099096	28.194796499115217	27.694613164984776	23.68876241490905
31	20.053166897827094	27.128668675371053	28.013853683421814	24.804310743380043
32	20.5585314139047	26.58902864795064	25.402139429591724	27.45030050855294
33	20.521466035359573	26.30246943102651	26.194063162593462	26.982001371020452
34	17.858737066972754	24.458367210292216	30.356545028456644	27.32635069427838
35	18.18435442472938	25.955330240566266	26.190874742933666	29.66944059177069
36	20.12092081559775	26.970443349753694	26.333955075166994	26.574680759481563
37	19.01812616576594	27.27015479777448	29.120235305370894	24.591483731088687
38	19.64305641908588	26.945733097390278	27.1326541999458	26.278556283578048
39	20.78849618186746	25.112391793007795	28.22389082851085	25.875221196613897
40	22.214516874711048	25.003985524574745	28.599725795909258	24.18177180480495
41	18.60642147719483	24.79514403685813	27.0218566167679	29.576577869179143
42	19.523092129386068	23.62140704959587	27.68345369617549	29.17204712484257
43	20.898098107672933	23.054665455067198	28.166499274634525	27.880737162625348
44	20.626285331675355	23.56720391537934	27.622076617724424	28.184434135220876
45	20.666539129880274	22.423358362427663	27.01308846270346	29.8970140449886
46	20.89690245030051	24.04745962663606	29.25016340650756	25.805474516555872
47	17.018987039074084	24.573548870502336	32.398329268098266	26.009134822325315
48	17.733990147783253	24.248728617660657	30.37846541361774	27.63881582093835
49	20.33414638034658	22.481547021218933	31.745898895212587	25.4384077032219
50	19.874615396878536	22.483539783506306	29.20672118864285	28.43512363097231
51	18.409138010744975	22.405423501841312	27.85283849060213	31.332599996811577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	116.5
2	175.0
3	565.0
4	955.0
5	678.5
6	402.0
7	453.5
8	505.0
9	602.5
10	700.0
11	722.5
12	745.0
13	710.0
14	675.0
15	695.5
16	716.0
17	697.5
18	679.0
19	725.0
20	771.0
21	843.0
22	915.0
23	1028.5
24	1142.0
25	1325.5
26	1839.0
27	2169.0
28	2479.5
29	2790.0
30	3240.5
31	3691.0
32	4207.0
33	4723.0
34	5267.5
35	5812.0
36	6349.5
37	6887.0
38	7501.5
39	8116.0
40	9109.0
41	10102.0
42	11621.5
43	13141.0
44	14448.5
45	15756.0
46	19849.5
47	23943.0
48	27227.5
49	30512.0
50	29505.5
51	28499.0
52	23557.0
53	18615.0
54	16457.0
55	14299.0
56	13312.5
57	12326.0
58	11313.5
59	10301.0
60	9498.0
61	8695.0
62	7647.5
63	6600.0
64	5664.5
65	4729.0
66	4019.0
67	3309.0
68	2862.5
69	2416.0
70	1946.0
71	1476.0
72	1231.5
73	987.0
74	768.0
75	406.0
76	263.0
77	185.5
78	108.0
79	96.0
80	84.0
81	60.0
82	36.0
83	27.5
84	19.0
85	11.5
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	250908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.538119026068784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59021208028277	36.18034659214838
2	10.8295279068265	10.946079407026627
3	5.973956847650729	9.057376266695139
4	4.119535386876154	8.327742788561977
5	2.779479509779048	7.023483314786643
6	1.7055082964496988	5.171590877155362
7	1.0805000826636606	3.8224509249733267
8	0.6441289228405562	2.6042451336519608
9	0.38409619092945313	1.747034911318711
>10	0.8163943841066158	6.363395665489686
>50	0.035564099193469166	1.2813056247393408
>100	0.03240284593182746	2.9923758542852683
>500	0.005532193207872982	1.6675345957876397
>1k	0.0031612532616417037	2.815038043379948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	2044	0.8146412230777815	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT	2026	0.8074672788432413	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	1609	0.6412709040763945	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	1369	0.5456183142825258	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	761	0.3032984201380586	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	622	0.24789962854910963	No Hit
TCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	591	0.23554450236740163	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC	570	0.22717490076043811	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	557	0.2219937188132702	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	539	0.21481977457873005	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.21322556474883225	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	448	0.17855150094855485	No Hit
GCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	419	0.16699347968179573	No Hit
CGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	386	0.15384124858513878	No Hit
TTCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	380	0.15144993384029207	No Hit
TGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	379	0.1510513813828176	No Hit
CGTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	289	0.11518166021011686	No Hit
TTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	284	0.11318889792274459	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC	256	0.10202942911345993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.985524574744528E-4	0.0	0.0	0.8473225245906866	0.0
2	3.985524574744528E-4	0.0	0.0	3.014650788336761	0.0
3	3.985524574744528E-4	0.0	0.0	4.349004415961229	0.0
4	3.985524574744528E-4	0.0	0.0	5.8750617756309085	0.0
5	3.985524574744528E-4	0.0	0.0	8.217354568208268	0.0
6	3.985524574744528E-4	0.0	0.0	9.47598322891259	0.0
7	3.985524574744528E-4	0.0	0.0	10.539719737911904	0.0
8	3.985524574744528E-4	0.0	0.0	11.982878186426898	0.0
9	3.985524574744528E-4	0.0	0.0	12.679149329634766	0.0
10	3.985524574744528E-4	0.0	0.0	14.110351204425527	0.0
11	3.985524574744528E-4	0.0	0.0	16.224273438870025	0.0
12	3.985524574744528E-4	0.0	0.0	18.13692668228992	0.0
13	3.985524574744528E-4	0.0	0.0	18.810878887879223	0.0
14	3.985524574744528E-4	0.0	0.0	19.113778755559807	0.0
15	3.985524574744528E-4	0.0	0.0	19.541824094887367	0.0
16	3.985524574744528E-4	0.0	0.0	20.388748067020583	0.0
17	3.985524574744528E-4	0.0	0.0	21.530202305227412	0.0
18	3.985524574744528E-4	0.0	0.0	22.631004192771854	0.0
19	3.985524574744528E-4	0.0	0.0	23.405391617644714	0.0
20	3.985524574744528E-4	0.0	0.0	24.182967462177373	0.0
21	3.985524574744528E-4	0.0	0.0	25.08768154064438	0.0
22	3.985524574744528E-4	0.0	0.0	25.991199961738964	0.0
23	3.985524574744528E-4	0.0	0.0	26.811819471678863	0.0
24	3.985524574744528E-4	0.0	0.0	27.49374272641765	0.0
25	3.985524574744528E-4	0.0	0.0	28.083201811022366	0.0
26	3.985524574744528E-4	0.0	0.0	28.606501187686323	0.0
27	3.985524574744528E-4	0.0	0.0	29.164474628150558	0.0
28	3.985524574744528E-4	0.0	0.0	29.70730307523076	0.0
29	3.985524574744528E-4	0.0	0.0	30.257704019002983	0.0
30	3.985524574744528E-4	0.0	0.0	30.85553270521466	0.0
31	3.985524574744528E-4	0.0	0.0	31.38002773925104	0.0
32	3.985524574744528E-4	0.0	0.0	31.898943038882777	0.0
33	3.985524574744528E-4	0.0	0.0	32.39514084843847	0.0
34	3.985524574744528E-4	0.0	0.0	32.879382084269935	0.0
35	3.985524574744528E-4	0.0	0.0	33.37677555119805	0.0
36	3.985524574744528E-4	0.0	0.0	33.861016787029506	0.0
37	3.985524574744528E-4	0.0	0.0	34.35043920480814	0.0
38	3.985524574744528E-4	0.0	0.0	34.86257911266281	0.0
39	3.985524574744528E-4	0.0	0.0	35.50982830360132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGGG	35	1.2068631E-7	45.000004	3
TTGCGCG	35	1.2068631E-7	45.000004	1
TCGCCAC	20	7.0227374E-4	45.0	42
CGTGGCA	25	3.8820624E-5	45.0	15
GCACGTG	25	3.8820624E-5	45.0	12
CGCGACC	25	3.8820624E-5	45.0	10
GCGTTAG	20	7.0227374E-4	45.0	1
TCTACGG	20	7.0227374E-4	45.0	2
GACGGGA	20	7.0227374E-4	45.0	4
AATTCCG	20	7.0227374E-4	45.0	11
TTGTGCG	60	0.0	44.999996	1
CGTGCGG	65	0.0	44.999996	2
GGCACGT	30	2.1586984E-6	44.999996	11
TTCGGGT	30	2.1586984E-6	44.999996	4
TGTGTCG	30	2.1586984E-6	44.999996	1
CGGGAAT	70	0.0	41.785717	6
CGTTTTT	410	0.0	41.15854	1
TATGGGA	55	6.002665E-11	40.909092	4
TCGGGAT	95	0.0	40.263157	5
TTTGGGC	485	0.0	39.896908	4
>>END_MODULE