SRR2933902.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592498
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	6883	0.432214043596915	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5528	0.3471275945087529	RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5186	0.32565190034775554	RNA PCR Primer, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4595	0.28854039377129514	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3801	0.2386816184384533	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2850	0.17896411800831147	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	2642	0.1659028771150733	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2357	0.14800646531424216	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	2285	0.14348526654350585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	2104	0.1321194751892938	No Hit
TCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1959	0.1230142832204499	RNA PCR Primer, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1918	0.12043971169822505	RNA PCR Primer, Index 16 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACCG	20	7.0341065E-4	45.0	1
ATCGCAT	20	7.0341065E-4	45.0	10
CGTCATA	70	0.0	41.785713	38
TATTGCG	65	0.0	41.53846	1
ACCGGTA	45	1.9290383E-8	40.0	41
CGTTTTT	1895	0.0	39.89446	1
TCGTTTG	445	0.0	39.4382	1
ATTCGCG	40	3.4593359E-7	39.375	1
GCGATTA	40	3.4593359E-7	39.375	9
CGTTAGG	150	0.0	39.000004	2
TTTGGGC	3240	0.0	38.680557	4
TTTGGGA	7505	0.0	38.614258	4
TACGAAT	140	0.0	38.571426	12
TTGGGTA	930	0.0	38.467743	5
TCTTGCG	560	0.0	38.16964	1
ACACGCG	295	0.0	38.135593	36
GCGCGAC	195	0.0	38.076923	9
CGGGCTA	95	0.0	37.894737	6
TTCGGGC	750	0.0	37.5	4
GTACGGG	180	0.0	37.5	3