##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933902.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592498
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44087653485279	33.0	31.0	34.0	31.0	34.0
2	32.664339295873525	34.0	31.0	34.0	31.0	34.0
3	32.720854908452004	34.0	31.0	34.0	31.0	34.0
4	36.10181990809408	37.0	35.0	37.0	35.0	37.0
5	36.12434803685782	37.0	35.0	37.0	35.0	37.0
6	35.729584589745166	37.0	35.0	37.0	35.0	37.0
7	36.05403648858586	37.0	35.0	37.0	35.0	37.0
8	36.06066004478499	37.0	36.0	37.0	35.0	37.0
9	37.92004950712654	39.0	38.0	39.0	35.0	39.0
10	37.62919827842798	39.0	37.0	39.0	35.0	39.0
11	37.5499435478098	39.0	37.0	39.0	35.0	39.0
12	37.234589933550936	39.0	37.0	39.0	35.0	39.0
13	37.088808902742734	39.0	37.0	39.0	33.0	39.0
14	37.93970290700522	40.0	37.0	41.0	33.0	41.0
15	38.093486459637624	40.0	37.0	41.0	33.0	41.0
16	38.2940776063769	40.0	37.0	41.0	33.0	41.0
17	38.31395581030557	40.0	37.0	41.0	34.0	41.0
18	38.29749111144881	40.0	37.0	41.0	34.0	41.0
19	38.311428334603875	40.0	37.0	41.0	34.0	41.0
20	38.20811203530554	40.0	37.0	41.0	34.0	41.0
21	38.150949640125134	40.0	36.0	41.0	34.0	41.0
22	38.21100685840736	40.0	36.0	41.0	34.0	41.0
23	38.17916192045453	40.0	36.0	41.0	34.0	41.0
24	38.138378823709665	40.0	36.0	41.0	34.0	41.0
25	38.0391554651874	40.0	36.0	41.0	34.0	41.0
26	37.98519621374721	40.0	36.0	41.0	34.0	41.0
27	37.965483159162524	40.0	36.0	41.0	34.0	41.0
28	37.885593576883615	40.0	36.0	41.0	34.0	41.0
29	37.85733420073369	40.0	36.0	41.0	34.0	41.0
30	37.758887609278005	40.0	36.0	41.0	34.0	41.0
31	37.638747426998336	40.0	35.0	41.0	33.0	41.0
32	37.568550164584195	40.0	35.0	41.0	33.0	41.0
33	37.51735512383689	40.0	35.0	41.0	33.0	41.0
34	37.40073080154575	40.0	35.0	41.0	33.0	41.0
35	37.36075021758269	40.0	35.0	41.0	33.0	41.0
36	37.270810387203	39.0	35.0	41.0	32.0	41.0
37	37.172397076793814	39.0	35.0	41.0	32.0	41.0
38	37.1492353522579	39.0	35.0	41.0	32.0	41.0
39	37.10476119907215	39.0	35.0	41.0	32.0	41.0
40	36.93431263335967	39.0	35.0	41.0	32.0	41.0
41	36.773251834539195	39.0	35.0	41.0	31.0	41.0
42	36.8110741740335	39.0	35.0	41.0	31.0	41.0
43	36.77128322924111	39.0	35.0	41.0	32.0	41.0
44	36.7081057558628	39.0	35.0	41.0	31.0	41.0
45	36.724453029140385	39.0	35.0	41.0	32.0	41.0
46	36.65104069204482	39.0	35.0	41.0	32.0	41.0
47	36.5533570528817	38.0	35.0	40.0	31.0	41.0
48	36.49056576523173	38.0	35.0	40.0	31.0	41.0
49	36.496753528104904	38.0	35.0	40.0	31.0	41.0
50	36.41307241830131	38.0	35.0	40.0	31.0	41.0
51	35.42654307886101	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	30.0
10	40.0
11	33.0
12	21.0
13	23.0
14	31.0
15	46.0
16	68.0
17	125.0
18	262.0
19	477.0
20	830.0
21	1298.0
22	2009.0
23	3071.0
24	4393.0
25	6306.0
26	8758.0
27	10600.0
28	13060.0
29	15758.0
30	19785.0
31	26471.0
32	36619.0
33	57735.0
34	120192.0
35	119862.0
36	111579.0
37	179866.0
38	337694.0
39	515172.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.178241982093542	4.695139334554894	54.520759209744696	15.605859473606873
2	20.7294451861164	5.210744377700945	52.04044212300424	22.019368313178415
3	21.327373723546277	5.166285923122038	50.84427107600763	22.662069277324054
4	19.662756248359496	4.930555642770038	50.21023574283924	25.19645236603123
5	19.569129757148833	5.9276055605721325	49.29167885925131	25.211585823027722
6	21.85151880881483	7.127418684356275	52.56835487391507	18.452707632913825
7	82.51690112012699	2.5291711512353547	10.000640503159188	4.953287225478462
8	83.67916317634308	3.5837407645095944	7.805472911111977	4.931623148035351
9	77.76361414582624	5.193601499028571	10.628898749009418	6.413885606135769
10	42.152078055985	25.47795978393693	17.76937867425893	14.600583485819135
11	32.49134378818686	22.772399086215493	25.591115342060082	19.14514178353756
12	30.133475834820516	19.953494447088787	29.52003707382992	20.392992644260776
13	28.45058518126867	20.01170488126202	31.56349332934798	19.974216608121328
14	21.43023099558053	22.32241421967249	34.431754388388555	21.815600396358427
15	19.71412208994925	22.50363893706617	36.711631662959704	21.07060731002488
16	25.919781374921662	22.74445556603525	29.82584593512833	21.509917123914754
17	25.797143858265443	23.282415425325496	28.01466626645685	22.90577444995221
18	26.022701441383283	22.61911788900206	30.138436594582853	21.219744075031805
19	25.841916284981203	24.439402749642387	28.514635497187435	21.20404546818897
20	24.226843612990407	28.72279902392342	28.18936036340391	18.86099699968226
21	24.613908463307332	25.91067618295282	31.284937249528728	18.19047810421112
22	22.952619092771233	22.115255403774448	32.63501743801248	22.29710806544184
23	21.926872121660434	23.76342073898994	31.523932840104035	22.78577429924559
24	23.146716667776033	25.253658089366514	28.647759683214673	22.951865559642776
25	23.857047230200603	24.783516211637313	26.778557963652073	24.580878594510008
26	20.710795241187117	24.44348438742152	28.306848737015684	26.538871634375678
27	20.400527975545337	23.97987313013894	31.395518236129654	24.22408065818607
28	18.316066958953794	25.293406961892572	29.398655445721122	26.99187063343251
29	20.479460570751108	25.089764633927324	29.146849791962058	25.283925003359503
30	23.46483323683923	23.893279614793865	28.379376300629577	24.262510847737328
31	22.26797145114154	26.198588632450402	28.19350479561042	23.33993512079764
32	24.25792685453922	25.74558963339357	25.778179941199298	24.218303570867906
33	24.155195171359715	24.010642399550893	25.682795206022234	26.151367223067158
34	19.55927103205153	25.152935827862894	30.261890438794897	25.025902701290676
35	19.186523311175275	27.522295161438194	27.34979886944913	25.941382657937407
36	22.033936620328564	26.452215324603234	25.9332821768065	25.5805658782617
37	19.492897322320026	29.56386758413511	25.833815803850303	25.109419289694557
38	21.185835084251284	27.458182051092056	25.289325324113438	26.06665754054322
39	20.43211357251312	24.453343112518823	25.85987549120941	29.254667823758652
40	22.8249580219253	24.17711042651231	27.691400554349205	25.306530997213184
41	19.46721440152515	26.20744264670976	25.98584111251631	28.339501839248776
42	19.03462359136401	24.362856342676725	29.27978559470718	27.322734471252087
43	19.95211296968662	22.72310546072899	29.569895849162762	27.75488572042163
44	21.232177371651332	22.415663944318926	28.185027547915286	28.16713113611446
45	20.25082606069207	22.20379554636803	26.93523005994356	30.610148332996335
46	20.44668187966327	23.56197621598269	28.837084881739255	27.154257022614786
47	17.464700112653205	24.827032749805653	31.60952164461117	26.098745492929975
48	18.04674165995813	26.036202243268125	29.176991117100304	26.740064979673445
49	21.524862197629133	22.645303165215903	29.768765800647788	26.06106883650717
50	19.969820998205336	23.3757279443993	27.532279475390236	29.122171582005123
51	18.449002761698914	24.291333489900772	26.488133737059638	30.771530011340676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	799.0
2	1410.0
3	3900.0
4	6390.0
5	4192.5
6	1995.0
7	2015.5
8	2036.0
9	2313.5
10	2591.0
11	2597.5
12	2604.0
13	2538.0
14	2472.0
15	2547.0
16	2622.0
17	2589.0
18	2556.0
19	2749.5
20	2943.0
21	3377.5
22	3812.0
23	4846.0
24	5880.0
25	7166.0
26	10553.0
27	12654.0
28	14984.0
29	17314.0
30	19858.0
31	22402.0
32	25565.5
33	28729.0
34	32552.0
35	36375.0
36	39178.5
37	41982.0
38	47109.5
39	52237.0
40	57982.5
41	63728.0
42	72169.5
43	80611.0
44	88414.5
45	96218.0
46	117075.5
47	137933.0
48	159377.5
49	180822.0
50	178126.0
51	175430.0
52	152236.0
53	129042.0
54	117363.5
55	105685.0
56	98303.0
57	90921.0
58	84934.5
59	78948.0
60	68630.0
61	58312.0
62	52014.0
63	45716.0
64	38935.0
65	32154.0
66	27411.5
67	22669.0
68	18878.5
69	15088.0
70	12069.5
71	9051.0
72	7537.5
73	6024.0
74	4682.5
75	2523.5
76	1706.0
77	1341.5
78	977.0
79	603.0
80	229.0
81	189.0
82	149.0
83	92.5
84	36.0
85	29.5
86	23.0
87	26.0
88	29.0
89	18.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1592498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.889156609112646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.45187673217362	19.369542669803767
2	12.27509597386591	6.846841480140689
3	4.792281438987877	4.009580627006297
4	2.384029663209521	2.6595430655208165
5	1.4801780364717319	2.0640458534264496
6	0.9706039924559426	1.6241596050620262
7	0.697230327443477	1.3611616056285814
8	0.5429521630414326	1.2113982325055181
9	0.42267904875535656	1.0609345967516008
>10	6.489355179805864	44.61383697617515
>50	0.4017986535105936	6.876648054555089
>100	0.08304544325660128	4.305410257212687
>500	0.0047779560888282085	0.876298198756607
>1k	0.0034128257777344346	2.004000703340417
>5k	6.82565155546887E-4	1.1165980741144108
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	6883	0.432214043596915	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5528	0.3471275945087529	RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5186	0.32565190034775554	RNA PCR Primer, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4595	0.28854039377129514	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3801	0.2386816184384533	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2850	0.17896411800831147	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	2642	0.1659028771150733	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2357	0.14800646531424216	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	2285	0.14348526654350585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	2104	0.1321194751892938	No Hit
TCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1959	0.1230142832204499	RNA PCR Primer, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1918	0.12043971169822505	RNA PCR Primer, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8838328211401208E-4	0.0	0.0	0.4518059049367723	0.0
2	1.8838328211401208E-4	0.0	0.0	1.4948213435746858	0.0
3	1.8838328211401208E-4	0.0	0.0	2.1590607963086925	0.0
4	1.8838328211401208E-4	0.0	0.0	2.8963301680755644	0.0
5	1.8838328211401208E-4	0.0	0.0	4.287038351068572	0.0
6	1.8838328211401208E-4	0.0	0.0	5.222800907756242	0.0
7	1.8838328211401208E-4	0.0	0.0	6.037935369463573	0.0
8	1.8838328211401208E-4	0.0	0.0	7.265252452436361	0.0
9	1.8838328211401208E-4	0.0	0.0	7.915237570157074	0.0
10	1.8838328211401208E-4	0.0	0.0	9.15511353860413	0.0
11	1.8838328211401208E-4	0.0	0.0	11.175461444849539	0.0
12	1.8838328211401208E-4	0.0	0.0	12.966421307907451	0.0
13	1.8838328211401208E-4	0.0	0.0	13.560896151831901	0.0
14	1.8838328211401208E-4	0.0	0.0	13.840205764779611	0.0
15	1.8838328211401208E-4	0.0	0.0	14.183565693646083	0.0
16	1.8838328211401208E-4	0.0	0.0	14.859736087580643	0.0
17	1.8838328211401208E-4	0.0	0.0	15.720459303559565	0.0
18	1.8838328211401208E-4	0.0	0.0	16.591041244635786	0.0
19	1.8838328211401208E-4	0.0	0.0	17.301748573624582	0.0
20	1.8838328211401208E-4	0.0	0.0	18.079394762191225	0.0
21	1.8838328211401208E-4	0.0	0.0	19.122410200829137	0.0
22	1.8838328211401208E-4	0.0	0.0	20.206242017258422	0.0
23	1.8838328211401208E-4	0.0	0.0	21.20065456911092	0.0
24	1.8838328211401208E-4	0.0	0.0	21.969949098837173	0.0
25	2.5117770948534943E-4	0.0	0.0	22.676386406764717	0.0
26	2.5117770948534943E-4	0.0	0.0	23.304644652614947	0.0
27	2.5117770948534943E-4	0.0	0.0	24.012023876953062	0.0
28	3.1397213685668677E-4	0.0	0.0	24.648319809506827	0.0
29	3.1397213685668677E-4	0.0	0.0	25.39174303515609	0.0
30	3.1397213685668677E-4	0.0	0.0	26.289954524275696	0.0
31	3.1397213685668677E-4	0.0	0.0	26.961729308294263	0.0
32	3.1397213685668677E-4	0.0	0.0	27.614791352956175	0.0
33	3.7676656422802417E-4	0.0	0.0	28.27538872890264	0.0
34	3.7676656422802417E-4	0.0	0.0	29.000413187332104	0.0
35	3.7676656422802417E-4	0.0	0.0	29.77686628177869	0.0
36	3.7676656422802417E-4	0.0	0.0	30.443868689317036	0.0
37	3.7676656422802417E-4	0.0	0.0	31.13812387833454	0.0
38	3.7676656422802417E-4	0.0	0.0	31.996272522791237	0.0
39	4.395609915993615E-4	0.0	0.0	33.670183573228975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACCG	20	7.0341065E-4	45.0	1
ATCGCAT	20	7.0341065E-4	45.0	10
CGTCATA	70	0.0	41.785713	38
TATTGCG	65	0.0	41.53846	1
ACCGGTA	45	1.9290383E-8	40.0	41
CGTTTTT	1895	0.0	39.89446	1
TCGTTTG	445	0.0	39.4382	1
ATTCGCG	40	3.4593359E-7	39.375	1
GCGATTA	40	3.4593359E-7	39.375	9
CGTTAGG	150	0.0	39.000004	2
TTTGGGC	3240	0.0	38.680557	4
TTTGGGA	7505	0.0	38.614258	4
TACGAAT	140	0.0	38.571426	12
TTGGGTA	930	0.0	38.467743	5
TCTTGCG	560	0.0	38.16964	1
ACACGCG	295	0.0	38.135593	36
GCGCGAC	195	0.0	38.076923	9
CGGGCTA	95	0.0	37.894737	6
TTCGGGC	750	0.0	37.5	4
GTACGGG	180	0.0	37.5	3
>>END_MODULE