SRR2933901.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616614
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	6829	0.42242613264514595	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	6060	0.3748575726796873	Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	5128	0.31720621001673865	RNA PCR Primer, Index 40 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	4424	0.27365839959322386	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.12872584302746357	No Hit
TCCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	1751	0.108312806891441	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1660526E-6	45.000004	9
TCTCGGC	20	7.0341385E-4	45.0	13
CGGTCTA	20	7.0341385E-4	45.0	31
TATTGCG	100	0.0	42.75	1
GGCGATA	190	0.0	41.44737	8
CGATATC	50	1.0822987E-9	40.5	10
TCTTGCG	485	0.0	39.432987	1
ACATACG	395	0.0	39.303795	17
CGTTTGG	1025	0.0	39.29268	2
CGTTTTT	1540	0.0	39.155846	1
TGGGCGA	1900	0.0	39.07895	6
CATACGA	395	0.0	38.734177	18
TTTTGCG	1305	0.0	38.620693	1
TTTGGGA	9815	0.0	38.604176	4
ACCGCGT	35	6.2498675E-6	38.571426	24
TTCGGGA	1750	0.0	38.057144	4
TTGGGAC	3395	0.0	37.776142	5
CGAATAT	340	0.0	37.72059	14
TTTGGGC	3450	0.0	37.630436	4
GTCGAAC	30	1.1401271E-4	37.500004	35