##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616614
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43726084272436	33.0	31.0	34.0	31.0	34.0
2	32.65714821225104	34.0	31.0	34.0	31.0	34.0
3	32.71905167219881	34.0	31.0	34.0	31.0	34.0
4	36.08820039910579	37.0	35.0	37.0	35.0	37.0
5	36.12490922384688	37.0	35.0	37.0	35.0	37.0
6	35.74980545757985	37.0	35.0	37.0	35.0	37.0
7	36.04426041095772	37.0	35.0	37.0	35.0	37.0
8	36.04941439329364	37.0	36.0	37.0	35.0	37.0
9	37.89325837831418	39.0	38.0	39.0	35.0	39.0
10	37.604811661905686	39.0	37.0	39.0	35.0	39.0
11	37.59518289461801	39.0	37.0	39.0	35.0	39.0
12	37.422583251165705	39.0	37.0	39.0	35.0	39.0
13	37.37214820606527	39.0	37.0	39.0	35.0	39.0
14	38.614573423216676	40.0	38.0	41.0	35.0	41.0
15	38.654788341558344	40.0	38.0	41.0	35.0	41.0
16	38.705678040645445	40.0	38.0	41.0	35.0	41.0
17	38.669407786892855	40.0	38.0	41.0	35.0	41.0
18	38.63001557576515	40.0	38.0	41.0	35.0	41.0
19	38.65263260122701	40.0	38.0	41.0	35.0	41.0
20	38.61847416884921	40.0	38.0	41.0	35.0	41.0
21	38.525456911792176	40.0	38.0	41.0	34.0	41.0
22	38.51459532083726	40.0	38.0	41.0	34.0	41.0
23	38.481969103323365	40.0	38.0	41.0	34.0	41.0
24	38.46450977165854	40.0	38.0	41.0	34.0	41.0
25	38.35938882132655	40.0	37.0	41.0	34.0	41.0
26	38.276412303741026	40.0	37.0	41.0	34.0	41.0
27	38.24064433439275	40.0	37.0	41.0	34.0	41.0
28	38.166946469596326	40.0	37.0	41.0	34.0	41.0
29	38.15852269001753	40.0	37.0	41.0	34.0	41.0
30	38.06934679521518	40.0	37.0	41.0	34.0	41.0
31	37.99349813870225	40.0	37.0	41.0	34.0	41.0
32	37.9303061831705	40.0	37.0	41.0	34.0	41.0
33	37.85428308798513	40.0	37.0	41.0	33.0	41.0
34	37.82118180344844	40.0	37.0	41.0	33.0	41.0
35	37.78283003858682	40.0	37.0	41.0	33.0	41.0
36	37.659169721405355	40.0	36.0	41.0	33.0	41.0
37	37.5889272269076	40.0	36.0	41.0	33.0	41.0
38	37.58682035414762	40.0	36.0	41.0	33.0	41.0
39	37.543042433134936	40.0	36.0	41.0	33.0	41.0
40	37.413975754261685	40.0	36.0	41.0	33.0	41.0
41	37.30422413761108	40.0	35.0	41.0	33.0	41.0
42	37.289372725956845	39.0	35.0	41.0	32.0	41.0
43	37.16306242553881	39.0	35.0	41.0	32.0	41.0
44	37.03632654424619	39.0	35.0	41.0	32.0	41.0
45	37.01301361982514	39.0	35.0	41.0	32.0	41.0
46	36.88602845206091	39.0	35.0	41.0	31.0	41.0
47	36.795563443097734	39.0	35.0	41.0	31.0	41.0
48	36.74131116023986	39.0	35.0	41.0	31.0	41.0
49	36.70949404124918	39.0	35.0	41.0	31.0	41.0
50	36.61790198526055	39.0	35.0	40.0	31.0	41.0
51	35.64247123926924	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	31.0
10	30.0
11	26.0
12	17.0
13	12.0
14	28.0
15	31.0
16	57.0
17	106.0
18	183.0
19	390.0
20	665.0
21	1136.0
22	1708.0
23	2511.0
24	3713.0
25	5532.0
26	7578.0
27	9942.0
28	11925.0
29	14337.0
30	18307.0
31	23718.0
32	31528.0
33	45086.0
34	86642.0
35	117561.0
36	114650.0
37	184984.0
38	356941.0
39	576827.0
40	401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.802519339805297	4.829167630615595	54.31704785434247	16.051265175236637
2	20.139563309485133	4.7269168768797005	53.02366551322702	22.109854300408138
3	21.608868907481934	4.67637914802173	51.38363270391076	22.33111924058557
4	20.059519464757823	5.0814851287938865	49.81888069755674	25.040114708891547
5	18.779498383658684	6.078445442140177	48.9218824035917	26.220173770609435
6	22.30612873574026	6.93430837540687	52.21834030881831	18.541222580034567
7	83.49185396142802	2.8663614196091336	8.767337162736435	4.8744474562264095
8	84.34196412996548	3.6415619312959064	6.989423572974129	5.027050365764493
9	77.77874000843738	6.309607611959317	9.966077245403046	5.945575134200248
10	35.772423101618564	32.29354688255824	17.090969149098054	14.843060866725144
11	30.844345032271153	24.94776118479736	24.635503589601477	19.57239019333001
12	29.234065769565277	21.473771722872623	28.645861040421522	20.646301467140578
13	25.990063181439726	21.744770241999635	30.258243464426265	22.006923112134373
14	21.095511977503598	24.639833627569725	29.94505800395147	24.319596390975214
15	19.400363970620074	25.253833011467176	34.003107730107494	21.342695287805252
16	23.02472946541351	24.251862225614772	31.143055794394954	21.580352514576763
17	21.12433765883507	25.023227560815382	29.351719086931077	24.500715693418464
18	21.928982428705925	24.559294921360326	30.2158709500227	23.29585169991105
19	23.775929195219142	27.036509642994556	28.14734995490575	21.040211206880556
20	27.393366629263387	26.039363756592483	27.718923626790314	18.848345987353817
21	26.073756629597415	26.518451528936403	27.630219706126507	19.777572135339668
22	22.98248066638047	24.82849956761478	28.520228081657095	23.668791684347653
23	23.389689808451493	25.360970522338665	28.160216353439964	23.089123315769875
24	23.926367085773105	23.099453549208405	28.22089874268069	24.7532806223378
25	22.331428529011873	24.871428801185687	26.516533940693325	26.28060872910911
26	21.585301129397617	26.935186754537572	27.62539480667618	23.854117309388638
27	21.468328246569683	27.693438260462916	28.152051138985556	22.68618235398184
28	19.94130942822467	28.248054266510124	28.55264150873369	23.257994796531516
29	22.487681041980338	26.448243056165545	28.025428457256957	23.038647444597164
30	23.34100781015134	27.03063316289479	27.39021188731509	22.238147139638777
31	22.84540403584282	29.440856011391713	25.371115182721415	22.342624770044058
32	25.014072623396803	28.800134107461645	24.425187459715183	21.76060580942637
33	23.69415333530453	27.705191220662446	25.52384180763002	23.076813636403003
34	22.31194335815476	27.484297426596576	26.902092892923108	23.301666322325552
35	23.367854045554473	26.335662068991113	25.87506974454013	24.421414140914283
36	21.840649654153683	30.676339559103162	24.64484410007584	22.838166686667318
37	21.969622927922188	30.973751309836484	25.86925512212563	21.1873706401157
38	22.674614966838096	28.828836073422597	25.551739623682586	22.94480933605672
39	23.10582489079026	26.888793490592068	25.960804496311425	24.04457712230625
40	23.866117700329205	26.46271775451654	27.399923543901018	22.271241001253237
41	20.953301159089307	27.151626795264672	26.92262964442965	24.97244240121637
42	22.79795919124788	25.539862948112535	25.87148199879501	25.79069586184457
43	22.389141749360082	25.1128593467581	26.721715882702984	25.776283021178838
44	22.3263561988205	25.624298688493358	26.450469932834924	25.59887517985122
45	23.37107064518803	24.958586279717977	25.29168991484671	26.378653160247286
46	22.381038392590934	27.458440914157617	26.31419745220566	23.84632324104579
47	20.716448082226183	27.322972583436737	28.192691638201822	23.767887696135258
48	21.28102317560036	25.48685091184414	28.608746429265118	24.623379483290382
49	22.522445061096835	25.049950080847992	28.455772373615467	23.971832484439698
50	21.403686965472275	25.211522354748876	27.2552384180763	26.12955226170255
51	19.89355529520343	24.455683298548696	26.412674887140653	29.23808651910722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	737.5
2	1290.0
3	2946.0
4	4602.0
5	3294.0
6	1986.0
7	1997.5
8	2009.0
9	2147.5
10	2286.0
11	2239.0
12	2192.0
13	2193.0
14	2194.0
15	2254.0
16	2314.0
17	2406.5
18	2499.0
19	2794.0
20	3089.0
21	3580.0
22	4071.0
23	4931.0
24	5791.0
25	7024.0
26	11608.5
27	14960.0
28	16156.5
29	17353.0
30	21122.0
31	24891.0
32	29445.0
33	33999.0
34	38215.5
35	42432.0
36	45171.5
37	47911.0
38	52968.0
39	58025.0
40	65989.0
41	73953.0
42	82870.5
43	91788.0
44	100934.0
45	110080.0
46	125518.5
47	140957.0
48	160675.5
49	180394.0
50	174804.5
51	169215.0
52	147434.0
53	125653.0
54	110154.5
55	94656.0
56	88712.5
57	82769.0
58	78603.5
59	74438.0
60	69742.5
61	65047.0
62	56382.0
63	47717.0
64	38961.5
65	30206.0
66	24039.5
67	17873.0
68	14865.5
69	11858.0
70	9466.5
71	7075.0
72	5740.0
73	4405.0
74	3560.0
75	2253.5
76	1792.0
77	1398.0
78	1004.0
79	693.0
80	382.0
81	306.5
82	231.0
83	130.5
84	30.0
85	25.5
86	21.0
87	17.5
88	14.0
89	9.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1616614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.4950504730698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.9898683318354	18.69384861441184
2	12.586868503536639	6.921531696052649
3	5.326854333474708	4.393863862847331
4	2.7023639061466302	2.972065279844146
5	1.6650963776062326	2.2890954472404528
6	1.1000302065645542	1.814723163083629
7	0.7448950845767949	1.43366495633064
8	0.5954467358324725	1.3097470444590793
9	0.4701667274900673	1.1634532112787708
>10	6.122199755431773	42.17230008000735
>50	0.6103128755604746	10.193059733406763
>100	0.0792896893873455	3.80711051121839
>500	0.003645502100363502	0.6356668327102459
>1k	0.0022784388127271888	1.0711803658729175
>5k	6.835316438181567E-4	1.1286892012358825
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	6829	0.42242613264514595	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	6060	0.3748575726796873	Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	5128	0.31720621001673865	RNA PCR Primer, Index 40 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	4424	0.27365839959322386	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.12872584302746357	No Hit
TCCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	1751	0.108312806891441	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7114611156404683E-4	0.0	0.0	0.44834450276936855	0.0
2	3.7114611156404683E-4	0.0	0.0	1.511801827770884	0.0
3	3.7114611156404683E-4	0.0	0.0	2.1547506083703345	0.0
4	3.7114611156404683E-4	0.0	0.0	2.8671037118322618	0.0
5	3.7114611156404683E-4	0.0	0.0	4.019636103609148	0.0
6	3.7114611156404683E-4	0.0	0.0	4.788960135196157	0.0
7	3.7114611156404683E-4	0.0	0.0	5.4788588989084595	0.0
8	3.7114611156404683E-4	0.0	0.0	6.627370541143402	0.0
9	3.7114611156404683E-4	0.0	0.0	7.160769361146198	0.0
10	3.7114611156404683E-4	0.0	0.0	8.243464426263783	0.0
11	3.7114611156404683E-4	0.0	0.0	10.191424792807684	0.0
12	3.7114611156404683E-4	0.0	0.0	12.029464052643364	0.0
13	3.7114611156404683E-4	0.0	0.0	12.611111867149486	0.0
14	3.7114611156404683E-4	0.0	0.0	12.82427345055777	0.0
15	3.7114611156404683E-4	0.0	0.0	13.159356531614844	0.0
16	3.7114611156404683E-4	0.0	0.0	13.897442432145212	0.0
17	3.7114611156404683E-4	0.0	0.0	14.878381605008988	0.0
18	3.7114611156404683E-4	0.0	0.0	15.90843577996974	0.0
19	3.7114611156404683E-4	0.0	0.0	16.692976802130875	0.0
20	3.7114611156404683E-4	0.0	0.0	17.579397431916338	0.0
21	3.7114611156404683E-4	0.0	0.0	18.804179600077692	0.0
22	3.7114611156404683E-4	0.0	0.0	20.119335846404894	0.0
23	3.7114611156404683E-4	0.0	0.0	21.343252006972598	0.0
24	3.7114611156404683E-4	0.0	0.0	22.231590225001145	0.0
25	3.7114611156404683E-4	0.0	0.0	23.05188498924295	0.0
26	3.7114611156404683E-4	0.0	0.0	23.751990271023264	0.0
27	3.7114611156404683E-4	0.0	0.0	24.406135292654895	0.0
28	4.948614820853958E-4	0.0	0.0	25.09158030302843	0.0
29	4.948614820853958E-4	0.0	0.0	25.839068571718418	0.0
30	4.948614820853958E-4	0.0	0.0	26.720726159738813	0.0
31	4.948614820853958E-4	0.0	0.0	27.519061445713078	0.0
32	4.948614820853958E-4	0.0	0.0	28.28127184349511	0.0
33	5.567191673460703E-4	0.0	0.0	29.035193311452208	0.0
34	5.567191673460703E-4	0.0	0.0	29.784413595329497	0.0
35	6.185768526067447E-4	0.0	0.0	30.54322182042219	0.0
36	6.804345378674192E-4	0.0	0.0	31.302710480052752	0.0
37	6.804345378674192E-4	0.0	0.0	32.050322464113265	0.0
38	6.804345378674192E-4	0.0	0.0	32.79972832104634	0.0
39	6.804345378674192E-4	0.0	0.0	33.64031240605364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1660526E-6	45.000004	9
TCTCGGC	20	7.0341385E-4	45.0	13
CGGTCTA	20	7.0341385E-4	45.0	31
TATTGCG	100	0.0	42.75	1
GGCGATA	190	0.0	41.44737	8
CGATATC	50	1.0822987E-9	40.5	10
TCTTGCG	485	0.0	39.432987	1
ACATACG	395	0.0	39.303795	17
CGTTTGG	1025	0.0	39.29268	2
CGTTTTT	1540	0.0	39.155846	1
TGGGCGA	1900	0.0	39.07895	6
CATACGA	395	0.0	38.734177	18
TTTTGCG	1305	0.0	38.620693	1
TTTGGGA	9815	0.0	38.604176	4
ACCGCGT	35	6.2498675E-6	38.571426	24
TTCGGGA	1750	0.0	38.057144	4
TTGGGAC	3395	0.0	37.776142	5
CGAATAT	340	0.0	37.72059	14
TTTGGGC	3450	0.0	37.630436	4
GTCGAAC	30	1.1401271E-4	37.500004	35
>>END_MODULE