##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688782
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.492520129736256	34.0	31.0	34.0	31.0	34.0
2	32.7214300025262	34.0	31.0	34.0	31.0	34.0
3	32.77507397115488	34.0	31.0	34.0	31.0	34.0
4	36.122583052402646	37.0	35.0	37.0	35.0	37.0
5	36.16716755083611	37.0	35.0	37.0	35.0	37.0
6	35.77364100687881	37.0	35.0	37.0	35.0	37.0
7	36.0988861497542	37.0	35.0	37.0	35.0	37.0
8	36.09830105897076	37.0	37.0	37.0	35.0	37.0
9	37.91648591281422	39.0	38.0	39.0	35.0	39.0
10	37.676729357038944	39.0	37.0	39.0	35.0	39.0
11	37.6348336629006	39.0	37.0	39.0	35.0	39.0
12	37.486121878910886	39.0	37.0	39.0	35.0	39.0
13	37.432098980519235	39.0	37.0	39.0	35.0	39.0
14	38.70634975943042	40.0	38.0	41.0	35.0	41.0
15	38.763803351423235	40.0	38.0	41.0	35.0	41.0
16	38.82880359823572	40.0	38.0	41.0	35.0	41.0
17	38.79781991980046	40.0	38.0	41.0	35.0	41.0
18	38.747699562415974	40.0	38.0	41.0	35.0	41.0
19	38.77288169551469	40.0	38.0	41.0	35.0	41.0
20	38.722180602861286	40.0	38.0	41.0	35.0	41.0
21	38.63725532897201	40.0	38.0	41.0	35.0	41.0
22	38.604247788124546	40.0	38.0	41.0	35.0	41.0
23	38.57078001457646	40.0	38.0	41.0	35.0	41.0
24	38.54447996608506	40.0	38.0	41.0	35.0	41.0
25	38.45371975458128	40.0	38.0	41.0	34.0	41.0
26	38.39897529261798	40.0	38.0	41.0	34.0	41.0
27	38.392626404290475	40.0	38.0	41.0	34.0	41.0
28	38.333240415690305	40.0	37.0	41.0	34.0	41.0
29	38.3225606940948	40.0	37.0	41.0	34.0	41.0
30	38.241289696885225	40.0	37.0	41.0	34.0	41.0
31	38.170821827515816	40.0	37.0	41.0	34.0	41.0
32	38.06052132605091	40.0	37.0	41.0	34.0	41.0
33	38.01562613424857	40.0	37.0	41.0	34.0	41.0
34	38.00617757142318	40.0	37.0	41.0	34.0	41.0
35	37.93595361086672	40.0	37.0	41.0	34.0	41.0
36	37.82292946099056	40.0	37.0	41.0	33.0	41.0
37	37.77631819646855	40.0	37.0	41.0	33.0	41.0
38	37.724110095792284	40.0	36.0	41.0	33.0	41.0
39	37.66418111971567	40.0	36.0	41.0	33.0	41.0
40	37.52162222590021	40.0	36.0	41.0	33.0	41.0
41	37.39372399394874	40.0	36.0	41.0	33.0	41.0
42	37.411578699791804	40.0	36.0	41.0	33.0	41.0
43	37.34697044928584	39.0	35.0	41.0	33.0	41.0
44	37.26804126704821	39.0	35.0	41.0	33.0	41.0
45	37.25229753390768	39.0	35.0	41.0	33.0	41.0
46	37.140941546091504	39.0	35.0	41.0	32.0	41.0
47	37.07881739069836	39.0	35.0	41.0	32.0	41.0
48	37.01033853962502	39.0	35.0	41.0	32.0	41.0
49	36.99201198637595	39.0	35.0	41.0	32.0	41.0
50	36.90540838755949	39.0	35.0	41.0	32.0	41.0
51	35.897821081271	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	23.0
11	16.0
12	11.0
13	11.0
14	7.0
15	10.0
16	25.0
17	48.0
18	69.0
19	150.0
20	240.0
21	381.0
22	646.0
23	964.0
24	1381.0
25	2129.0
26	2996.0
27	3819.0
28	4444.0
29	5371.0
30	6745.0
31	8874.0
32	12117.0
33	17468.0
34	33724.0
35	51270.0
36	47357.0
37	76916.0
38	153614.0
39	257784.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.953442453490364	4.75128560270158	54.51957223040091	16.775699713407143
2	20.186357947797706	4.74678490436742	53.19694765542654	21.869909492408336
3	20.98559486165434	4.646753254295263	51.45779070881643	22.909861175233964
4	19.35895537339826	5.168108341971771	50.21646907149142	25.256467213138556
5	18.821484882009113	6.221997671251572	48.9620808906156	25.994436556123706
6	22.573034719258054	7.1669991376081255	51.113705061978976	19.14626108115485
7	83.10031330667759	2.7534110937858425	9.108106773986545	5.038168825550029
8	83.28847153380896	3.674892781750975	7.665850733613828	5.370784950826241
9	76.37685073071016	6.83394746088022	10.719937512885064	6.0692642955245635
10	36.33689614420818	31.844328103812238	17.55649828247544	14.26227746950414
11	28.71140651178457	25.47743698296413	26.279722756982615	19.531433748268682
12	27.60481545684992	22.183361353810056	29.979441971480092	20.23238121785993
13	24.462166549067774	24.008902671672605	30.631462494664497	20.897468284595124
14	20.282034083352933	26.343168085112563	30.231916629644793	23.14288120188971
15	17.216187414885987	26.747069464649194	34.65624827594217	21.380494844522648
16	20.19869857226234	24.930384359637735	32.69176604498956	22.17915102311036
17	20.81064836189099	25.152370416183928	29.278784869523307	24.758196352401775
18	20.746186747040426	24.881602597048122	31.043494167966063	23.32871648794539
19	23.870252126217004	25.994291372306478	28.652752249623248	21.48270425185327
20	26.403564553080656	24.929803624368812	28.960687125970193	19.705944696580342
21	25.22394603807881	26.154283938895034	28.16899977060957	20.452770252416585
22	22.381392080513137	25.088634720419524	28.352076564137857	24.177896634929485
23	22.234175689840907	25.744720390486396	28.09815006780084	23.922953851871856
24	22.71429857342381	23.87402690546501	29.818723485805375	23.592951035305802
25	21.382237050329422	26.102017764691876	28.067371098547873	24.448374086430828
26	20.61827980405992	27.670293358421098	28.885917460096227	22.825509377422755
27	21.920578644621955	27.261310545281383	28.32826641811197	22.489844391984693
28	19.901942849842186	26.1232146020076	30.594150253636126	23.380692294514084
29	21.324453891071485	24.060878478241303	30.06858483525992	24.54608279542729
30	22.465889062141578	25.169647290434423	29.67731444782239	22.687149199601617
31	23.433829571620628	26.652119248180124	26.996495262652044	22.917555917547205
32	23.425989645490155	26.097807433992177	28.483322734914672	21.992880185602992
33	23.49858155410565	24.666730547546248	29.398271151104417	22.436416747243683
34	21.563716821868166	23.15275370146142	30.62376775235125	24.65976172431916
35	22.293701054905615	22.61499284243781	30.5771637470201	24.51414235563647
36	22.8041673562898	24.636967865013894	29.919771422598153	22.639093356098154
37	22.828558237584605	25.75589954441318	29.599931473238268	21.815610744763948
38	21.552973219393074	25.16224291575564	28.769915590128665	24.514868274722627
39	22.658112436155417	23.653057135639433	31.117537914753868	22.57129251345128
40	23.780383343351016	22.570276226730666	32.221806028612825	21.427534401305493
41	20.191584565218022	23.850942678525282	31.431570511424518	24.52590224483218
42	21.40503090963469	23.158270686516197	30.951883179293304	24.48481522455581
43	22.116141246432107	23.291694614551485	30.327012029931094	24.265152109085314
44	22.582036115926375	22.718799271757973	29.13432697137846	25.56483764093719
45	22.3029928192084	22.59176343168085	28.439332038293685	26.665911710817063
46	22.528463287368137	23.8955141104152	30.129852406131402	23.446170196085266
47	20.95510626003583	22.98971227471101	32.55006663937211	23.505114825881048
48	20.891515748088654	21.665055126295403	32.8942974700268	24.54913165558914
49	22.351774581798015	20.90574376217729	33.15040172362228	23.592079932402417
50	21.856552581223088	21.063413387690154	31.540313190530533	25.539720840556228
51	20.386421247942017	21.96021382672602	29.331341411360924	28.32202351397104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	458.5
2	743.0
3	1304.5
4	1866.0
5	1404.5
6	943.0
7	919.5
8	896.0
9	979.5
10	1063.0
11	1071.0
12	1079.0
13	1017.0
14	955.0
15	1074.0
16	1193.0
17	1178.0
18	1163.0
19	1232.0
20	1301.0
21	1466.0
22	1631.0
23	1978.5
24	2326.0
25	2943.5
26	4802.0
27	6043.0
28	7322.0
29	8601.0
30	9939.0
31	11277.0
32	13107.0
33	14937.0
34	17655.5
35	20374.0
36	21320.0
37	22266.0
38	25303.5
39	28341.0
40	31740.5
41	35140.0
42	39698.5
43	44257.0
44	47366.5
45	50476.0
46	56290.0
47	62104.0
48	69582.5
49	77061.0
50	74858.5
51	72656.0
52	60235.5
53	47815.0
54	42395.5
55	36976.0
56	34051.0
57	31126.0
58	29788.0
59	28450.0
60	26109.0
61	23768.0
62	20481.0
63	17194.0
64	14197.0
65	11200.0
66	8947.0
67	6694.0
68	5706.0
69	4718.0
70	3901.5
71	3085.0
72	2696.0
73	2307.0
74	1969.5
75	1209.0
76	786.0
77	535.0
78	284.0
79	232.5
80	181.0
81	148.0
82	115.0
83	62.0
84	9.0
85	7.0
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	688782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.413705626176327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.85982778560142	19.56562876169276
2	12.755384916510343	7.248555043325893
3	5.089537645467637	4.3383787329498
4	2.5844427386833404	2.937343807386295
5	1.3989852215241292	1.987517712987884
6	0.9495409944499582	1.6187986977772708
7	0.686282373752555	1.3649877740967031
8	0.5149941279501012	1.1706313240626849
9	0.4138677614435445	1.0583565067639296
>10	6.177079529210706	44.46356011850733
>50	0.49660129690578136	8.5067369021213
>100	0.06879997134201758	3.3960217728103474
>500	0.002069172070436619	0.4390355782464621
>1k	0.002586465088045774	1.9044472672713122
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3877	0.5628776594045721	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	3050	0.4428106425545383	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2492	0.36179807253964247	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2480	0.3600558667328705	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1058	0.15360447863039395	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.12660028862542866	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	831	0.12064775211895781	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	720	0.10453234840631725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4518381723099615E-4	0.0	0.0	0.46342674460133976	0.0
2	1.4518381723099615E-4	0.0	0.0	1.849061096253967	0.0
3	1.4518381723099615E-4	0.0	0.0	2.6034362105862234	0.0
4	1.4518381723099615E-4	0.0	0.0	3.360424633628637	0.0
5	1.4518381723099615E-4	0.0	0.0	4.58955083030625	0.0
6	1.4518381723099615E-4	0.0	0.0	5.351765870768981	0.0
7	1.4518381723099615E-4	0.0	0.0	6.0312261354100425	0.0
8	1.4518381723099615E-4	0.0	0.0	7.112845573780964	0.0
9	1.4518381723099615E-4	0.0	0.0	7.642476139039638	0.0
10	1.4518381723099615E-4	0.0	0.0	8.6470029704609	0.0
11	1.4518381723099615E-4	0.0	0.0	10.43537723111231	0.0
12	1.4518381723099615E-4	0.0	0.0	12.093957159159212	0.0
13	1.4518381723099615E-4	0.0	0.0	12.633315040172363	0.0
14	1.4518381723099615E-4	0.0	0.0	12.843541207522845	0.0
15	1.4518381723099615E-4	0.0	0.0	13.184723178015686	0.0
16	1.4518381723099615E-4	0.0	0.0	13.962618070739364	0.0
17	1.4518381723099615E-4	0.0	0.0	15.00561861372684	0.0
18	1.4518381723099615E-4	0.0	0.0	16.10320827199317	0.0
19	1.4518381723099615E-4	0.0	0.0	16.83464434320293	0.0
20	1.4518381723099615E-4	0.0	0.0	17.659288425074987	0.0
21	1.4518381723099615E-4	0.0	0.0	18.747876686672996	0.0
22	1.4518381723099615E-4	0.0	0.0	19.91573531247913	0.0
23	1.4518381723099615E-4	0.0	0.0	20.995467361226048	0.0
24	1.4518381723099615E-4	0.0	0.0	21.781928099166354	0.0
25	2.903676344619923E-4	0.0	0.0	22.489844391984693	0.0
26	2.903676344619923E-4	0.0	0.0	23.106730431399196	0.0
27	2.903676344619923E-4	0.0	0.0	23.74466812431219	0.0
28	2.903676344619923E-4	0.0	0.0	24.406996698519997	0.0
29	2.903676344619923E-4	0.0	0.0	25.060178692242246	0.0
30	2.903676344619923E-4	0.0	0.0	25.85026902561333	0.0
31	2.903676344619923E-4	0.0	0.0	26.564283038755367	0.0
32	2.903676344619923E-4	0.0	0.0	27.26392385399154	0.0
33	2.903676344619923E-4	0.0	0.0	27.91841250206887	0.0
34	2.903676344619923E-4	0.0	0.0	28.565206407832957	0.0
35	2.903676344619923E-4	0.0	0.0	29.226373511502914	0.0
36	2.903676344619923E-4	0.0	0.0	29.90365601888551	0.0
37	2.903676344619923E-4	0.0	0.0	30.57280823250317	0.0
38	2.903676344619923E-4	0.0	0.0	31.21219776358848	0.0
39	2.903676344619923E-4	0.0	0.0	31.876704095054748	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	25	3.889171E-5	45.000004	24
CAAACGA	25	3.889171E-5	45.000004	29
ACACGAC	35	1.210974E-7	45.000004	26
CGTGATA	25	3.889171E-5	45.000004	13
TCTAGCG	35	1.210974E-7	45.000004	1
CGTTCAT	35	1.210974E-7	45.000004	17
ACGATTG	25	3.889171E-5	45.000004	10
AAACGCG	25	3.889171E-5	45.000004	13
GTCAAGC	35	1.210974E-7	45.000004	16
AATCGTT	25	3.889171E-5	45.000004	22
CTAGCGG	35	1.210974E-7	45.000004	2
CCCGAAC	25	3.889171E-5	45.000004	25
TACGGTT	40	6.8084773E-9	45.0	33
CGTTCGG	45	3.8380676E-10	45.0	2
AACGGGT	20	7.031316E-4	45.0	19
GTATCGC	20	7.031316E-4	45.0	23
TCGTACG	45	3.8380676E-10	45.0	30
CAAGCCG	20	7.031316E-4	45.0	27
CGTGTAT	20	7.031316E-4	45.0	24
CCGAACG	20	7.031316E-4	45.0	26
>>END_MODULE