FastQCFastQC Report
Sat 14 Jan 2017
SRR2933898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877131
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC36880.42046170982441616TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC31140.3550210857899219No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT30680.34977671522269765Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG27140.3094178634662325No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC27140.3094178634662325TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC25820.294368800099415TruSeq Adapter, Index 23 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC22680.25857027057531884TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.13384545752002835No Hit
TCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG9800.11172789469303902Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG302.1649103E-645.0000041
GATTCGC207.032372E-445.011
TACACGG207.032372E-445.016
TACGTTG207.032372E-445.017
ACGAACG253.8900478E-545.021
CGATTCG700.041.78571310
ACAAGCG700.041.78571314
TGTTTCG2750.040.9090921
TTTCGCG1900.040.2631571
CTTACGG900.040.0000042
CGGGATC2700.040.06
TGCGTTG1350.040.01
TTGGGCA11200.039.575895
TTGCACG403.4569712E-739.3751
TTTGGGC20550.038.7591254
CGTTTTT7850.038.6942671
TGGGCGA8900.038.6797756
GGGCGAT13350.038.4269687
TTGGGAC15500.037.8870965
AACGGCG301.1397012E-437.50000414
Produced by FastQC (version 0.11.5)