##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933898.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877131
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48122458332906	33.0	31.0	34.0	31.0	34.0
2	32.69948844585358	34.0	31.0	34.0	31.0	34.0
3	32.763750226591014	34.0	31.0	34.0	31.0	34.0
4	36.12510217972002	37.0	35.0	37.0	35.0	37.0
5	36.15526187080379	37.0	35.0	37.0	35.0	37.0
6	35.73872431826033	37.0	35.0	37.0	35.0	37.0
7	36.0703942740594	37.0	35.0	37.0	35.0	37.0
8	36.05979380503026	37.0	36.0	37.0	35.0	37.0
9	37.94691556905411	39.0	38.0	39.0	35.0	39.0
10	37.649021639869076	39.0	37.0	39.0	35.0	39.0
11	37.55879338434054	39.0	37.0	39.0	35.0	39.0
12	37.395948837744875	39.0	37.0	39.0	35.0	39.0
13	37.34492453236746	39.0	37.0	39.0	35.0	39.0
14	38.630847615692524	40.0	38.0	41.0	35.0	41.0
15	38.68203609267031	40.0	38.0	41.0	35.0	41.0
16	38.7464973875054	40.0	38.0	41.0	35.0	41.0
17	38.70231812579877	40.0	38.0	41.0	35.0	41.0
18	38.653017622225185	40.0	38.0	41.0	35.0	41.0
19	38.64700825760348	40.0	38.0	41.0	35.0	41.0
20	38.584806602434526	40.0	38.0	41.0	35.0	41.0
21	38.52190721796402	40.0	38.0	41.0	34.0	41.0
22	38.50953620382816	40.0	38.0	41.0	34.0	41.0
23	38.45197353645008	40.0	38.0	41.0	34.0	41.0
24	38.433775570581815	40.0	37.0	41.0	34.0	41.0
25	38.341836054135584	40.0	37.0	41.0	34.0	41.0
26	38.28445693972736	40.0	37.0	41.0	34.0	41.0
27	38.265857665502644	40.0	37.0	41.0	34.0	41.0
28	38.21051587505173	40.0	37.0	41.0	34.0	41.0
29	38.19539612669031	40.0	37.0	41.0	34.0	41.0
30	38.134604751171715	40.0	37.0	41.0	34.0	41.0
31	38.08055467199312	40.0	37.0	41.0	34.0	41.0
32	38.00414647298978	40.0	37.0	41.0	34.0	41.0
33	37.93879705539994	40.0	37.0	41.0	34.0	41.0
34	37.89976867765476	40.0	37.0	41.0	34.0	41.0
35	37.854881425921555	40.0	37.0	41.0	34.0	41.0
36	37.75926856991715	40.0	36.0	41.0	33.0	41.0
37	37.69363983259057	40.0	36.0	41.0	33.0	41.0
38	37.65847746801789	40.0	36.0	41.0	33.0	41.0
39	37.61154263160235	40.0	36.0	41.0	33.0	41.0
40	37.49574008899469	40.0	36.0	41.0	33.0	41.0
41	37.368291623486115	40.0	35.0	41.0	33.0	41.0
42	37.34678742399938	40.0	35.0	41.0	33.0	41.0
43	37.259828919511456	39.0	35.0	41.0	33.0	41.0
44	37.15314018088518	39.0	35.0	41.0	33.0	41.0
45	37.144699024433066	39.0	35.0	41.0	33.0	41.0
46	37.0686533710472	39.0	35.0	41.0	33.0	41.0
47	36.985721631090456	39.0	35.0	41.0	32.0	41.0
48	36.939711400007525	39.0	35.0	41.0	32.0	41.0
49	36.93459015814057	39.0	35.0	41.0	32.0	41.0
50	36.83815074373155	39.0	35.0	41.0	32.0	41.0
51	35.9094034984512	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	24.0
10	23.0
11	18.0
12	10.0
13	11.0
14	11.0
15	19.0
16	31.0
17	59.0
18	94.0
19	241.0
20	412.0
21	663.0
22	1075.0
23	1526.0
24	2122.0
25	3028.0
26	3995.0
27	5111.0
28	5971.0
29	7180.0
30	9209.0
31	11908.0
32	15824.0
33	23871.0
34	46549.0
35	65809.0
36	59596.0
37	95698.0
38	185957.0
39	330887.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.321531219395965	4.865065765546993	53.24723444958621	16.566168565470836
2	19.3728188833823	6.391861648944114	52.7787753482661	21.45654411940748
3	20.609692280856564	6.359369353038486	51.128508740427606	21.90242962567735
4	19.5558018129561	4.626104880570861	51.549084458307824	24.26900884816521
5	19.56629055409055	5.915992023996416	49.15069698824919	25.367020433663846
6	21.656628257352665	7.759616294487369	51.997592149861305	18.586163298298658
7	83.17902342979555	2.695492463497471	9.60780088721069	4.5176832194962895
8	84.1128634149289	4.469343803833179	6.810499229875583	4.607293551362339
9	78.99515579770868	5.040638171493198	8.928426882643528	7.035779148154608
10	41.189628459146924	25.212881542209775	17.58904884219119	16.008441156452115
11	29.335298832215486	25.303403938522294	24.907567968752673	20.453729260509547
12	27.479589707808756	21.31357801742271	29.25492315286998	21.951909121898552
13	24.530771344303187	22.615664022819853	31.220992075300043	21.632572557576918
14	20.709449329689637	24.9239851287892	29.940111568283417	24.42645397323775
15	19.200552711054563	24.612287104206786	33.736693834786365	22.45046634995229
16	21.186573043251236	24.01636699649197	30.977014835868307	23.82004512438849
17	21.6892345613141	24.650137778735445	28.454016560810185	25.206611099140265
18	21.845197581661118	24.250881567291547	30.15615683404189	23.74776401700544
19	23.326960283013598	26.186852362987967	27.627572164249127	22.858615189749308
20	25.71235083470998	25.441695710218887	28.987574261997352	19.858379193073784
21	25.804811367971258	25.5815835946968	27.98658353199237	20.627021505339567
22	23.751982315070382	23.47824897307244	28.38583974343627	24.38392896842091
23	22.772539107613344	25.708474560812466	27.800180360744292	23.718805970829898
24	23.896202505669052	23.14922172400702	28.765486569280984	24.189089201042947
25	21.74965883089299	26.359346551427326	27.121262388400364	24.76973222927932
26	21.565877844928522	26.433451787703323	28.14425667317653	23.85641369419163
27	22.191440047153733	26.78824485738162	28.134908012600167	22.885407082864475
28	20.286365434581608	25.10309178446549	30.463636560559372	24.146906220393532
29	21.375940423950357	23.477222900570155	30.68321607604793	24.463620599431557
30	23.481441198635096	24.76562793927019	28.893061583731505	22.85986927836321
31	22.92861613601617	26.235533802818505	27.129698984530247	23.706151076635077
32	23.105670646687894	25.006412953139268	28.75898811009986	23.128928290072974
33	23.919346140998325	23.670694571278407	27.206198389978237	25.20376089774504
34	21.424393847669275	23.35272610362648	30.096758636965287	25.126121411738957
35	21.84770575888892	22.73810867475896	30.286696057943452	25.12748950840866
36	23.19037863215415	24.48653621864921	28.00630692564737	24.316778223549278
37	22.390042080373398	24.721050789448782	29.45318316192222	23.435723968255598
38	21.58822342386713	24.322706642451354	28.990652479504202	25.098417454177312
39	22.450922382175527	23.811380512146986	30.34347206973645	23.39422503594104
40	23.910111488477774	22.835813578587462	30.605690598097663	22.6483843348371
41	20.119571648932713	23.845810945001375	30.72790723392515	25.30671017214076
42	21.33307339496609	22.418430086269897	29.93885747966951	26.309639039094503
43	21.963309927479475	22.53403425486045	28.689215179944615	26.813440637715463
44	21.996372263664153	22.531298061521028	28.931368290483405	26.540961384331418
45	22.337484366645345	22.24160359170979	28.585239833046604	26.83567220859826
46	23.39981143067569	23.237463959203357	29.57391769302419	23.78880691709676
47	20.07317037021836	23.418166727660978	31.946995374693177	24.56166752742749
48	20.74524785921373	22.192124095488587	31.536110341556732	25.526517703740947
49	21.559037361579968	21.245743224216223	32.39550306624666	24.799716347957144
50	21.609428922247645	21.403758389567805	30.624615935361994	26.362196752822552
51	19.9612144594137	21.25988022313657	29.248652709800478	29.530252607649253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	493.0
2	836.0
3	1579.5
4	2323.0
5	1665.0
6	1007.0
7	1023.0
8	1039.0
9	1142.5
10	1246.0
11	1277.0
12	1308.0
13	1293.0
14	1278.0
15	1250.5
16	1223.0
17	1297.5
18	1372.0
19	1468.0
20	1564.0
21	1808.0
22	2052.0
23	2338.0
24	2624.0
25	3686.0
26	6051.5
27	7355.0
28	8663.5
29	9972.0
30	11196.5
31	12421.0
32	14841.5
33	17262.0
34	19391.0
35	21520.0
36	23941.5
37	26363.0
38	28847.5
39	31332.0
40	35212.5
41	39093.0
42	44119.5
43	49146.0
44	54018.5
45	58891.0
46	70808.0
47	82725.0
48	87744.5
49	92764.0
50	90653.0
51	88542.0
52	76945.5
53	65349.0
54	58173.0
55	50997.0
56	47925.0
57	44853.0
58	43048.0
59	41243.0
60	37545.0
61	33847.0
62	29810.5
63	25774.0
64	22775.5
65	19777.0
66	16197.5
67	12618.0
68	10436.0
69	8254.0
70	7153.0
71	6052.0
72	4671.0
73	3290.0
74	2806.5
75	1786.0
76	1249.0
77	1021.5
78	794.0
79	559.5
80	325.0
81	225.5
82	126.0
83	99.5
84	73.0
85	46.5
86	20.0
87	12.5
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	877131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.800198766445227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17024387085354	19.507467270996628
2	12.199106698128338	6.782751819620822
3	4.607568228935714	3.8427393778311254
4	2.2523993993129046	2.5046860400928233
5	1.2863670410013377	1.7880629713220588
6	0.8364139942168327	1.3951485174158589
7	0.5992864659386518	1.1662198009794518
8	0.48244938383885155	1.0729751012375282
9	0.3770905331105742	0.9434872596076881
>10	6.680410053524155	47.731517351875965
>50	0.445185014501242	7.423316939538137
>100	0.05559626424490065	2.5608303687082437
>500	0.004563872438014233	0.8214680608026353
>1k	0.003319179954919442	2.4593291199710463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	3688	0.42046170982441616	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	3114	0.3550210857899219	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	3068	0.34977671522269765	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	2714	0.3094178634662325	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2714	0.3094178634662325	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	2582	0.294368800099415	TruSeq Adapter, Index 23 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2268	0.25857027057531884	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1174	0.13384545752002835	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	980	0.11172789469303902	Illumina PCR Primer Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1400805580922348E-4	0.0	0.0	0.36208958525009377	0.0
2	1.1400805580922348E-4	0.0	0.0	1.4225925203874905	0.0
3	1.1400805580922348E-4	0.0	0.0	2.013040241423459	0.0
4	1.1400805580922348E-4	0.0	0.0	2.6657363609312634	0.0
5	1.1400805580922348E-4	0.0	0.0	3.9129844914841683	0.0
6	1.1400805580922348E-4	0.0	0.0	4.541738919272036	0.0
7	1.1400805580922348E-4	0.0	0.0	5.167529137608863	0.0
8	1.1400805580922348E-4	0.0	0.0	6.083355849924356	0.0
9	1.1400805580922348E-4	0.0	0.0	6.491048657498139	0.0
10	1.1400805580922348E-4	0.0	0.0	7.6380837069947365	0.0
11	1.1400805580922348E-4	0.0	0.0	9.254375914202097	0.0
12	1.1400805580922348E-4	0.0	0.0	11.05821137321563	0.0
13	1.1400805580922348E-4	0.0	0.0	11.53396699010752	0.0
14	1.1400805580922348E-4	0.0	0.0	11.714783766620949	0.0
15	1.1400805580922348E-4	0.0	0.0	12.035374419556486	0.0
16	1.1400805580922348E-4	0.0	0.0	12.686930458506199	0.0
17	1.1400805580922348E-4	0.0	0.0	13.517023112853154	0.0
18	1.1400805580922348E-4	0.0	0.0	14.401725625932729	0.0
19	1.1400805580922348E-4	0.0	0.0	15.118836296972743	0.0
20	1.1400805580922348E-4	0.0	0.0	15.816337582413574	0.0
21	1.1400805580922348E-4	0.0	0.0	16.814706127134944	0.0
22	1.1400805580922348E-4	0.0	0.0	17.812504631577266	0.0
23	1.1400805580922348E-4	0.0	0.0	18.719324707483832	0.0
24	1.1400805580922348E-4	0.0	0.0	19.450344361332572	0.0
25	1.1400805580922348E-4	0.0	0.0	20.143171316485223	0.0
26	1.1400805580922348E-4	0.0	0.0	20.732136932795672	0.0
27	1.1400805580922348E-4	0.0	0.0	21.293968631823525	0.0
28	1.1400805580922348E-4	0.0	0.0	21.887380562310533	0.0
29	1.1400805580922348E-4	0.0	0.0	22.505532240908142	0.0
30	1.1400805580922348E-4	0.0	0.0	23.204287614962873	0.0
31	1.1400805580922348E-4	0.0	0.0	23.854361549187065	0.0
32	1.1400805580922348E-4	0.0	0.0	24.445949350781127	0.0
33	1.1400805580922348E-4	0.0	0.0	25.048481925732872	0.0
34	2.2801611161844695E-4	0.0	0.0	25.64599814622901	0.0
35	2.2801611161844695E-4	0.0	0.0	26.28797750849075	0.0
36	3.4202416742767043E-4	0.0	0.0	26.908637364316164	0.0
37	3.4202416742767043E-4	0.0	0.0	27.50524152036583	0.0
38	3.4202416742767043E-4	0.0	0.0	28.100249563634165	0.0
39	3.4202416742767043E-4	0.0	0.0	28.733222289486974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	30	2.1649103E-6	45.000004	1
GATTCGC	20	7.032372E-4	45.0	11
TACACGG	20	7.032372E-4	45.0	16
TACGTTG	20	7.032372E-4	45.0	17
ACGAACG	25	3.8900478E-5	45.0	21
CGATTCG	70	0.0	41.785713	10
ACAAGCG	70	0.0	41.785713	14
TGTTTCG	275	0.0	40.909092	1
TTTCGCG	190	0.0	40.263157	1
CTTACGG	90	0.0	40.000004	2
CGGGATC	270	0.0	40.0	6
TGCGTTG	135	0.0	40.0	1
TTGGGCA	1120	0.0	39.57589	5
TTGCACG	40	3.4569712E-7	39.375	1
TTTGGGC	2055	0.0	38.759125	4
CGTTTTT	785	0.0	38.694267	1
TGGGCGA	890	0.0	38.679775	6
GGGCGAT	1335	0.0	38.426968	7
TTGGGAC	1550	0.0	37.887096	5
AACGGCG	30	1.1397012E-4	37.500004	14
>>END_MODULE