FastQCFastQC Report
Sat 14 Jan 2017
SRR2933897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085988
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT113301.0432896127765683No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC99230.9137301701307934No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC89360.8228451879762945No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC82380.758571917921745No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC51360.47293340257903405No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC46700.4300231678434753No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG45600.4198941424767125No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG32600.3001874790513339No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG30960.28508602304997843No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC22390.20617170723801737No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC21420.19723974850550835No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT19770.18204621045536415No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG18360.16906264157615No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG17170.15810487777028842No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.15442159581873832No Hit
TTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG16120.1484362626474694No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT14310.13176941181670515No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC12500.1151025609859409No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT12350.11372133025410963No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT12320.11344508410774336No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT12190.11224801747348957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG207.033116E-445.033
AACGCGA253.890664E-545.012
TCGTCCC1000.045.038
GTAAGCG253.890664E-545.019
TATCGCG253.890664E-545.01
CGATAGA253.890664E-545.010
ATACCGG207.033116E-445.022
CTCGTCC1050.042.85714337
TATCGAG603.6379788E-1241.2500041
TTATGCG556.184564E-1140.909091
CTAGGTG950.040.2631574
TGGGCGA11800.040.233056
CGTTTTT13450.039.981411
CTTACCG403.4579898E-739.3751
TTGGGAC23400.039.230775
TTTGGGC23950.038.7056354
TCACGGG1050.038.571433
TCCGATC356.2479703E-638.5714314
TTACCGG700.038.571432
TCGCTTG1600.037.968751
Produced by FastQC (version 0.11.5)