##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933897.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085988
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30481920610541	33.0	31.0	34.0	31.0	34.0
2	32.519117154149036	34.0	31.0	34.0	31.0	34.0
3	32.58431400715293	34.0	31.0	34.0	31.0	34.0
4	36.00026519630051	37.0	35.0	37.0	35.0	37.0
5	36.03765327057021	37.0	35.0	37.0	35.0	37.0
6	35.62362107131939	37.0	35.0	37.0	35.0	37.0
7	35.97365164255959	37.0	35.0	37.0	35.0	37.0
8	35.96034486568912	37.0	35.0	37.0	35.0	37.0
9	37.836964128517074	39.0	38.0	39.0	35.0	39.0
10	37.52402696899045	39.0	37.0	39.0	35.0	39.0
11	37.474547600894304	39.0	37.0	39.0	35.0	39.0
12	37.324703403720854	39.0	37.0	39.0	35.0	39.0
13	37.229020946824456	39.0	37.0	39.0	34.0	39.0
14	38.25463448951554	40.0	38.0	41.0	33.0	41.0
15	38.311213383573296	40.0	38.0	41.0	33.0	41.0
16	38.454576846152996	40.0	38.0	41.0	34.0	41.0
17	38.45008876709503	40.0	38.0	41.0	34.0	41.0
18	38.4304697657801	40.0	38.0	41.0	34.0	41.0
19	38.45289911122406	40.0	38.0	41.0	34.0	41.0
20	38.36920205379801	40.0	38.0	41.0	34.0	41.0
21	38.307949074943735	40.0	37.0	41.0	34.0	41.0
22	38.341334342552585	40.0	37.0	41.0	34.0	41.0
23	38.28069555096373	40.0	37.0	41.0	34.0	41.0
24	38.25551663554293	40.0	37.0	41.0	34.0	41.0
25	38.18399558742822	40.0	37.0	41.0	34.0	41.0
26	38.133669064483215	40.0	37.0	41.0	34.0	41.0
27	38.124293270275544	40.0	37.0	41.0	34.0	41.0
28	38.06655598404402	40.0	37.0	41.0	34.0	41.0
29	38.04272607063798	40.0	37.0	41.0	34.0	41.0
30	37.99025771923815	40.0	37.0	41.0	34.0	41.0
31	37.90353944979134	40.0	37.0	41.0	34.0	41.0
32	37.7317392089047	40.0	36.0	41.0	33.0	41.0
33	37.73147677506565	40.0	36.0	41.0	33.0	41.0
34	37.70568090991797	40.0	36.0	41.0	33.0	41.0
35	37.612129231630554	40.0	36.0	41.0	33.0	41.0
36	37.541980206042794	40.0	36.0	41.0	33.0	41.0
37	37.44652795426837	40.0	36.0	41.0	33.0	41.0
38	37.391476701400016	40.0	36.0	41.0	33.0	41.0
39	37.38477036578673	40.0	36.0	41.0	33.0	41.0
40	37.27651870923067	39.0	35.0	41.0	32.0	41.0
41	37.107739680364794	39.0	35.0	41.0	32.0	41.0
42	37.112346545265694	39.0	35.0	41.0	32.0	41.0
43	37.059226252960435	39.0	35.0	41.0	32.0	41.0
44	36.972058623115544	39.0	35.0	41.0	32.0	41.0
45	37.018595048932404	39.0	35.0	41.0	32.0	41.0
46	36.93740998979731	39.0	35.0	41.0	32.0	41.0
47	36.825904153637055	39.0	35.0	41.0	31.0	41.0
48	36.78383094472499	39.0	35.0	41.0	31.0	41.0
49	36.79137798944372	39.0	35.0	41.0	31.0	41.0
50	36.718991370070384	39.0	35.0	41.0	31.0	41.0
51	35.72280080442878	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	22.0
11	32.0
12	19.0
13	19.0
14	21.0
15	19.0
16	47.0
17	86.0
18	148.0
19	279.0
20	519.0
21	888.0
22	1485.0
23	2187.0
24	2880.0
25	4131.0
26	5385.0
27	6745.0
28	7857.0
29	9741.0
30	12872.0
31	17070.0
32	23538.0
33	34844.0
34	63861.0
35	73398.0
36	80503.0
37	129124.0
38	249829.0
39	358237.0
40	186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.696887995079134	4.3801588967833895	54.5331992618703	14.389753846267178
2	20.084383989510012	6.736078115043628	50.32053761183365	22.85900028361271
3	20.955388089002824	6.782119139438005	49.996408800097235	22.26608397146193
4	19.500952128384476	4.349219328390369	51.82304040191973	24.32678814130543
5	19.769555464701266	5.6485891188484585	49.154502628021675	25.427352788428603
6	20.328309336751417	7.846127213192043	52.57875777632902	19.24680567372752
7	77.32359841913538	2.3029720402067055	13.79379882650637	6.579630714151538
8	76.98768310515402	4.491209847622626	10.781795010626269	7.739312036597089
9	71.39498778992032	5.0757466933336275	14.024464358722197	9.504801158023845
10	35.98391510771758	25.435824336917168	22.01285833729286	16.56740221807239
11	27.26678379503273	24.451283071267824	27.3431198134786	20.938813320220852
12	24.519147541225134	22.13762951340162	31.960021657697872	21.383201287675373
13	24.365554683845495	23.676688876856836	33.47891505246835	18.47884138682932
14	20.3931350990987	26.222297115621906	31.67852683455066	21.706040950728735
15	16.948898146204193	26.222020869475536	34.83012703639451	21.99895394792576
16	19.96928142852407	26.742744855375932	28.363573078155557	24.924400637944434
17	20.64046748214529	26.773684423768955	28.06255686066513	24.523291233420625
18	21.51911439168757	24.42863088726579	31.18947907343359	22.86277564761305
19	21.279332736641656	26.35609233251196	28.865328162005472	23.49924676884091
20	21.98652287133928	28.57757175954062	30.22390670983473	19.21199865928537
21	23.21784402774248	27.956294176362906	30.472988651808308	18.352873144086306
22	21.13430350979937	25.170443872307978	29.679701801493202	24.015550816399443
23	20.060442656824936	26.50830395915977	29.10317609402682	24.32807728998847
24	22.39168388600979	25.956640404866356	27.241829559810977	24.409846149312884
25	20.42407466749172	28.145706950721372	26.19375168049739	25.236466701289515
26	19.993406925306726	25.694482811964775	29.41478174712796	24.89732851560054
27	21.56340585715496	25.504517545313576	29.428962382641426	23.503114214890036
28	16.727256654769665	25.36667071827681	29.839648320239263	28.066424306714254
29	19.119179954106308	25.782973660850768	28.858790336541475	26.239056048501457
30	23.10762181534234	25.70516432962427	26.916135353245156	24.271078501788235
31	22.78966250087478	27.131883593557205	25.00027624614637	25.07817765942165
32	21.581730184863922	30.290850359304155	25.124402847913608	23.00301660791832
33	22.25291623848514	25.53582544190175	26.641454601708308	25.569803717904804
34	19.86817533895402	25.106815176594953	29.891674677804914	25.13333480664611
35	20.052247354482738	26.808399356162315	28.04340379451707	25.095949494837882
36	22.942610783912897	25.278087787342034	28.327845243225525	23.451456185519547
37	21.148023735068897	27.504907973200442	28.21882009745964	23.128248194271023
38	19.195976382796125	29.325738405949238	26.00553597277318	25.472749238481455
39	21.0022578518363	25.91907092896054	28.742490708921274	24.33618051028188
40	23.694644876370642	24.27945796822801	27.94855928426465	24.0773378711367
41	20.770026924791065	25.82238477773235	26.55323999896868	26.8543482985079
42	20.424442995686874	24.527803253811275	30.032928540646857	25.01482520985499
43	21.49600179744159	23.141231763150238	30.615715827430872	24.747050611977297
44	21.030711204912027	22.92235273317937	27.837416251376627	28.209519810531976
45	21.19701138502451	22.09407470432454	26.52239251262445	30.1865213980265
46	22.565442712074166	23.259649277892574	29.297837545166246	24.877070464867014
47	18.093662176745966	24.04897660010976	33.12292585185103	24.734435371293237
48	18.49744196068465	24.62808060494223	30.487353451419352	26.38712398295377
49	21.590293815401278	21.667090244091096	32.12309896610276	24.619516974404874
50	21.467916772561022	21.837349952301498	28.72048309926076	27.974250175876712
51	19.630143242835096	21.866632043816324	26.830222801725252	31.673001911623334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	447.0
2	693.0
3	1801.5
4	2910.0
5	2114.0
6	1318.0
7	1386.0
8	1454.0
9	1650.5
10	1847.0
11	1926.0
12	2005.0
13	2197.5
14	2390.0
15	2264.5
16	2139.0
17	2368.0
18	2597.0
19	2693.5
20	2790.0
21	3165.5
22	3541.0
23	4151.5
24	4762.0
25	5587.0
26	7323.5
27	8235.0
28	9169.5
29	10104.0
30	12382.5
31	14661.0
32	16790.0
33	18919.0
34	21914.5
35	24910.0
36	27615.0
37	30320.0
38	33573.0
39	36826.0
40	41312.5
41	45799.0
42	52181.0
43	58563.0
44	67632.0
45	76701.0
46	103673.5
47	130646.0
48	145249.0
49	159852.0
50	149015.5
51	138179.0
52	110482.0
53	82785.0
54	70130.0
55	57475.0
56	50305.0
57	43135.0
58	38125.0
59	33115.0
60	28525.5
61	23936.0
62	20749.5
63	17563.0
64	15693.0
65	13823.0
66	11850.0
67	9877.0
68	8118.5
69	6360.0
70	5292.0
71	4224.0
72	3298.0
73	2372.0
74	1790.5
75	1013.5
76	818.0
77	549.5
78	281.0
79	222.5
80	164.0
81	106.5
82	49.0
83	30.0
84	11.0
85	9.0
86	7.0
87	5.5
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1085988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.66567533287935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88508067980207	24.919448681238563
2	12.651100841923723	8.77117908779289
3	4.715928309016985	4.904425189605526
4	2.2886300190401747	3.1734762078851273
5	1.2681394074490036	2.198045448772858
6	0.8591443758986314	1.7869691999382726
7	0.5974168573989317	1.4496901171886314
8	0.4950476888879691	1.372892996582608
9	0.4177610910086731	1.303377331285451
>10	4.615742130566605	33.916262137637766
>50	0.12751713995561284	2.9569686395038564
>100	0.06697223469055077	4.375079895057918
>500	0.005893556649897088	1.5621836044889237
>1k	0.004286223018106973	3.264501263143451
>5k	0.0010715557545267432	2.993333974764682
>10k+	2.678889386316858E-4	1.0521662251135124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	11330	1.0432896127765683	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	9923	0.9137301701307934	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	8936	0.8228451879762945	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	8238	0.758571917921745	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	5136	0.47293340257903405	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	4670	0.4300231678434753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	4560	0.4198941424767125	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	3260	0.3001874790513339	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	3096	0.28508602304997843	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	2239	0.20617170723801737	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	2142	0.19723974850550835	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	1977	0.18204621045536415	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1836	0.16906264157615	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1717	0.15810487777028842	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1677	0.15442159581873832	No Hit
TTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1612	0.1484362626474694	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	1431	0.13176941181670515	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTC	1250	0.1151025609859409	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	1235	0.11372133025410963	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	1232	0.11344508410774336	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	1219	0.11224801747348957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1180602363930356	0.0
2	0.0	0.0	0.0	3.8578695160535843	0.0
3	0.0	0.0	0.0	5.358898993359043	0.0
4	0.0	0.0	0.0	6.961494970478495	0.0
5	0.0	0.0	0.0	9.64099050818241	0.0
6	0.0	0.0	0.0	11.013841773573926	0.0
7	0.0	0.0	0.0	12.287889000615108	0.0
8	0.0	0.0	0.0	14.018755271697293	0.0
9	0.0	0.0	0.0	14.770789364155037	0.0
10	0.0	0.0	0.0	16.674217394667345	0.0
11	0.0	0.0	0.0	19.417617874230654	0.0
12	0.0	0.0	0.0	22.088733945494795	0.0
13	0.0	0.0	0.0	22.934967973863433	0.0
14	0.0	0.0	0.0	23.280275656821253	0.0
15	0.0	0.0	0.0	23.836174985358955	0.0
16	0.0	0.0	0.0	24.94180414516551	0.0
17	0.0	0.0	0.0	26.353606117194666	0.0
18	0.0	0.0	0.0	27.785666139957346	0.0
19	0.0	0.0	0.0	28.973800815478626	0.0
20	0.0	0.0	0.0	30.03357311498838	0.0
21	0.0	0.0	0.0	31.463146922433765	0.0
22	0.0	0.0	0.0	32.913255026759046	0.0
23	0.0	0.0	0.0	34.32303119371485	0.0
24	0.0	0.0	0.0	35.38667093927373	0.0
25	0.0	0.0	0.0	36.30509729389275	0.0
26	0.0	0.0	0.0	37.13061286128392	0.0
27	0.0	0.0	0.0	37.970032818042185	0.0
28	0.0	0.0	0.0	38.8205947027039	0.0
29	0.0	0.0	0.0	39.716921365613615	0.0
30	0.0	0.0	0.0	40.655421606868586	0.0
31	0.0	0.0	0.0	41.47578057952758	0.0
32	0.0	0.0	0.0	42.249085625255525	0.0
33	0.0	0.0	0.0	43.01097249693367	0.0
34	0.0	0.0	0.0	43.78123883505158	0.0
35	0.0	0.0	0.0	44.57931395190371	0.0
36	0.0	0.0	0.0	45.28604367635738	0.0
37	0.0	0.0	0.0	46.02039801544768	0.0
38	0.0	0.0	0.0	46.78559984088222	0.0
39	0.0	0.0	0.0	47.77870473706892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGACG	20	7.033116E-4	45.0	33
AACGCGA	25	3.890664E-5	45.0	12
TCGTCCC	100	0.0	45.0	38
GTAAGCG	25	3.890664E-5	45.0	19
TATCGCG	25	3.890664E-5	45.0	1
CGATAGA	25	3.890664E-5	45.0	10
ATACCGG	20	7.033116E-4	45.0	22
CTCGTCC	105	0.0	42.857143	37
TATCGAG	60	3.6379788E-12	41.250004	1
TTATGCG	55	6.184564E-11	40.90909	1
CTAGGTG	95	0.0	40.263157	4
TGGGCGA	1180	0.0	40.23305	6
CGTTTTT	1345	0.0	39.98141	1
CTTACCG	40	3.4579898E-7	39.375	1
TTGGGAC	2340	0.0	39.23077	5
TTTGGGC	2395	0.0	38.705635	4
TCACGGG	105	0.0	38.57143	3
TCCGATC	35	6.2479703E-6	38.57143	14
TTACCGG	70	0.0	38.57143	2
TCGCTTG	160	0.0	37.96875	1
>>END_MODULE