##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933895.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518067
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.372679595496336	33.0	31.0	34.0	31.0	34.0
2	32.60383309494718	34.0	31.0	34.0	31.0	34.0
3	32.6551758749351	34.0	31.0	34.0	31.0	34.0
4	36.039363634433386	37.0	35.0	37.0	35.0	37.0
5	36.08770487214974	37.0	35.0	37.0	35.0	37.0
6	35.70018163673811	37.0	35.0	37.0	35.0	37.0
7	36.04145023713149	37.0	35.0	37.0	35.0	37.0
8	36.05731691074707	37.0	36.0	37.0	35.0	37.0
9	37.92612538532661	39.0	38.0	39.0	35.0	39.0
10	37.534733924376575	39.0	37.0	39.0	35.0	39.0
11	37.47129618369825	39.0	37.0	39.0	35.0	39.0
12	37.329144685919005	39.0	37.0	39.0	35.0	39.0
13	37.26155690287164	39.0	37.0	39.0	34.0	39.0
14	38.47702324216752	40.0	38.0	41.0	34.0	41.0
15	38.535816795897055	40.0	38.0	41.0	34.0	41.0
16	38.585789096777056	40.0	38.0	41.0	35.0	41.0
17	38.583492096582106	40.0	38.0	41.0	35.0	41.0
18	38.54868192724107	40.0	38.0	41.0	35.0	41.0
19	38.579689499620706	40.0	38.0	41.0	35.0	41.0
20	38.50852109862238	40.0	38.0	41.0	34.0	41.0
21	38.425385133583106	40.0	38.0	41.0	34.0	41.0
22	38.41798068589584	40.0	37.0	41.0	34.0	41.0
23	38.36276195936047	40.0	37.0	41.0	34.0	41.0
24	38.336576157137976	40.0	37.0	41.0	34.0	41.0
25	38.270206363269615	40.0	37.0	41.0	34.0	41.0
26	38.2309701254857	40.0	37.0	41.0	34.0	41.0
27	38.22399419380119	40.0	37.0	41.0	34.0	41.0
28	38.17426124420201	40.0	37.0	41.0	34.0	41.0
29	38.17349686430519	40.0	37.0	41.0	34.0	41.0
30	38.110887201848406	40.0	37.0	41.0	34.0	41.0
31	38.023363773411546	40.0	37.0	41.0	34.0	41.0
32	37.93602371893983	40.0	37.0	41.0	34.0	41.0
33	37.87453746330108	40.0	37.0	41.0	34.0	41.0
34	37.86941264353838	40.0	37.0	41.0	34.0	41.0
35	37.84422671198899	40.0	37.0	41.0	34.0	41.0
36	37.75796373828096	40.0	36.0	41.0	33.0	41.0
37	37.6871466431948	40.0	36.0	41.0	33.0	41.0
38	37.65302557391225	40.0	36.0	41.0	33.0	41.0
39	37.616628737209666	40.0	36.0	41.0	33.0	41.0
40	37.52248261325273	40.0	36.0	41.0	33.0	41.0
41	37.431172029872585	40.0	36.0	41.0	33.0	41.0
42	37.44596934373353	40.0	36.0	41.0	33.0	41.0
43	37.3619628349229	40.0	36.0	41.0	33.0	41.0
44	37.280496538092564	39.0	35.0	41.0	33.0	41.0
45	37.30183740713074	39.0	35.0	41.0	33.0	41.0
46	37.22643982342052	39.0	35.0	41.0	33.0	41.0
47	37.14735738813705	39.0	35.0	41.0	32.0	41.0
48	37.1146357517464	39.0	35.0	41.0	32.0	41.0
49	37.14261668857503	39.0	35.0	41.0	32.0	41.0
50	37.06837532597135	39.0	35.0	41.0	32.0	41.0
51	36.145396251836154	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	12.0
11	11.0
12	10.0
13	4.0
14	3.0
15	5.0
16	13.0
17	29.0
18	47.0
19	86.0
20	195.0
21	258.0
22	481.0
23	744.0
24	1107.0
25	1697.0
26	2374.0
27	2957.0
28	3494.0
29	4488.0
30	5558.0
31	7476.0
32	10012.0
33	14530.0
34	27357.0
35	39955.0
36	35274.0
37	55834.0
38	114632.0
39	189331.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.95179387994217	4.590911986287488	57.276568474733956	16.18072565903638
2	18.678665114743843	5.133312872659328	54.328301165679335	21.859720846917483
3	20.08137943547842	4.697654936523654	54.53425908231947	20.686706545678454
4	19.258705920276718	5.32904045229671	52.019912482362315	23.39234114506425
5	18.037242287194513	6.242821874390764	51.3603452835251	24.359590554889618
6	19.62661200192253	7.060476733704328	54.556456983363155	18.756454281009987
7	81.47343876371202	2.6367245935371297	10.222615993684215	5.667220649066627
8	83.2249496686722	3.30825935641529	7.650554851013479	5.816236123899032
9	77.8107850915036	4.95688781566863	10.942986138858487	6.289340953969274
10	37.136702395636085	24.952178000142837	21.521154599694633	16.38996500452644
11	25.531639729996314	26.672032767962445	26.023081956580906	21.773245545460338
12	23.064005234844142	22.0925092700365	32.8712309411717	21.972254553947657
13	21.64391864372755	24.541806368674322	32.90018472514173	20.9140902624564
14	19.169142215196104	26.088710533579633	31.622550751157668	23.119596500066596
15	16.409460552399594	25.205813147720274	36.123899032364534	22.260827267515594
16	18.059633213464668	25.072432716231685	31.667911679377376	25.200022390926268
17	18.53698459851718	25.16411969880344	29.5152943538191	26.78360134886028
18	18.809150167835433	24.481389472790198	33.13162197167548	23.577838387698886
19	21.353222652668478	25.53723746156385	30.64063142412082	22.46890846164685
20	22.32085811294678	26.001849181669552	32.07789726039296	19.599395444990705
21	22.772151092426267	26.732063613393635	30.597007722939313	19.898777571240785
22	20.705043942192805	25.022439182576772	29.269766265753272	25.002750609477154
23	19.091932124609365	26.09392221469424	29.4396284650441	25.374517195652302
24	20.35894971113775	23.92103724035694	31.076868435935896	24.64314461256942
25	18.526947286740906	26.210509451480213	28.85900858383182	26.40353467794706
26	17.65003368290202	26.344661983874673	29.48923594824608	26.51606838497723
27	18.171008769136037	26.860039338541153	31.549394190326733	23.419557701996073
28	16.257549699170184	24.414988794885602	35.46220855603619	23.865252949908022
29	19.710771000662078	22.14288885414435	32.36280249465803	25.78353765053555
30	21.25883331692619	24.040133805086985	31.64629285401309	23.054740023973732
31	20.360107862496548	27.301295006244363	29.8017437898959	22.536853341363184
32	20.184261881185254	26.29775685384323	27.556088305180605	25.961892959790916
33	20.201827176793735	26.17151835573391	28.377603669023504	25.249050798448845
34	17.99574186350414	24.428500560738282	31.223181557597762	26.352576018159812
35	18.20420910808834	23.904823121333727	28.62699226161867	29.263975508959266
36	20.03312312886171	24.624807216055068	30.717455464254623	24.624614190828602
37	18.575203593357617	25.042320780902855	34.818276400542786	21.564199225196738
38	20.182138603694117	26.261854161720393	30.450115525598044	23.105891708987446
39	22.86827765520676	24.381016355027437	30.372133334105435	22.378572655660367
40	22.789330337581816	22.648228897034553	30.4404642642747	24.12197650110893
41	17.36840987748689	24.02951741763131	29.725112774988567	28.876959929893236
42	18.669785954326372	24.753941092561387	28.440915943304628	28.13535700980761
43	19.980234216809794	24.71340579500335	30.362096022329162	24.9442639658577
44	20.671843603240507	22.373360974545765	30.092246755728507	26.862548666485225
45	22.141151627106144	21.860492947823353	28.21005777245028	27.78829765262022
46	22.03923430753165	21.923226146425076	31.742805467246516	24.294734078796758
47	17.790363022543414	22.81249336475784	34.65980268961351	24.737340923085238
48	18.348785002712003	22.64417536727875	32.70175479233381	26.305284837675437
49	21.067545317497544	20.967558250187718	33.98440742220601	23.98048901010873
50	22.066450864463476	20.6299571290972	31.27703559578201	26.02655641065731
51	18.9846100986938	20.722223187348355	28.632396967959746	31.660769745998103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	189.0
2	329.0
3	918.5
4	1508.0
5	1065.5
6	623.0
7	675.0
8	727.0
9	800.5
10	874.0
11	947.5
12	1021.0
13	934.5
14	848.0
15	870.0
16	892.0
17	839.0
18	786.0
19	1197.5
20	1609.0
21	1462.0
22	1315.0
23	1553.5
24	1792.0
25	2138.0
26	3447.0
27	4410.0
28	5306.0
29	6202.0
30	7109.0
31	8016.0
32	9173.0
33	10330.0
34	12363.5
35	14397.0
36	15315.5
37	16234.0
38	18228.5
39	20223.0
40	22557.0
41	24891.0
42	29205.0
43	33519.0
44	38719.0
45	43919.0
46	50470.0
47	57021.0
48	65373.5
49	73726.0
50	69151.0
51	64576.0
52	52490.0
53	40404.0
54	32977.0
55	25550.0
56	21713.0
57	17876.0
58	16199.5
59	14523.0
60	12430.0
61	10337.0
62	8300.5
63	6264.0
64	5405.0
65	4546.0
66	3498.5
67	2451.0
68	2052.5
69	1654.0
70	1416.5
71	1179.0
72	847.0
73	515.0
74	380.5
75	153.5
76	61.0
77	57.5
78	54.0
79	30.5
80	7.0
81	21.0
82	35.0
83	36.5
84	38.0
85	19.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.55747086346553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.66769792919962	20.371962370058387
2	13.674751176229002	8.357316212655173
3	5.870874713541053	5.381972490062617
4	3.070767926790075	3.7533960580540864
5	1.793991593346532	2.740992292149438
6	1.1631670311221498	2.132606559771528
7	0.8389306400450729	1.7944919012752063
8	0.5872154313787519	1.4355054747946843
9	0.4405515487102166	1.2115927002210352
>10	5.541504491789241	40.24047689218985
>50	0.275202149713286	5.2054886661716
>100	0.06832164737661624	3.685343151041988
>500	0.0031926003447016937	0.6528567441437151
>1k	0.003831120413642032	3.0359984874107013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	3869	0.7468146012002308	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	3511	0.6777115701250996	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2980	0.5752151748712039	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2374	0.4582418876322947	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1734	0.3347057426935126	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	1092	0.21078354730179688	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	805	0.1553853073058118	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.15442018117347756	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	620	0.1196756404094451	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	603	0.11639421155950871	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9302522646684695E-4	0.0	0.0	0.7020327486599224	0.0
2	1.9302522646684695E-4	0.0	0.0	2.6114382888699725	0.0
3	1.9302522646684695E-4	0.0	0.0	3.638525518900065	0.0
4	1.9302522646684695E-4	0.0	0.0	4.667156950741893	0.0
5	1.9302522646684695E-4	0.0	0.0	6.275443137663661	0.0
6	1.9302522646684695E-4	0.0	0.0	7.293072131596879	0.0
7	1.9302522646684695E-4	0.0	0.0	8.254337759401777	0.0
8	1.9302522646684695E-4	0.0	0.0	9.830195708277115	0.0
9	1.9302522646684695E-4	0.0	0.0	10.47760231784692	0.0
10	1.9302522646684695E-4	0.0	0.0	11.824725373359044	0.0
11	3.860504529336939E-4	0.0	0.0	14.479787363410525	0.0
12	3.860504529336939E-4	0.0	0.0	16.99799446789701	0.0
13	3.860504529336939E-4	0.0	0.0	17.785730417108212	0.0
14	3.860504529336939E-4	0.0	0.0	18.032609681759308	0.0
15	3.860504529336939E-4	0.0	0.0	18.39182962821411	0.0
16	3.860504529336939E-4	0.0	0.0	19.33205550633412	0.0
17	3.860504529336939E-4	0.0	0.0	20.749053693827246	0.0
18	3.860504529336939E-4	0.0	0.0	22.43300576952402	0.0
19	3.860504529336939E-4	0.0	0.0	23.517421491814765	0.0
20	3.860504529336939E-4	0.0	0.0	24.701631256188872	0.0
21	3.860504529336939E-4	0.0	0.0	26.266100716702667	0.0
22	3.860504529336939E-4	0.0	0.0	27.80489782209637	0.0
23	3.860504529336939E-4	0.0	0.0	29.299492150629167	0.0
24	3.860504529336939E-4	0.0	0.0	30.36923795570843	0.0
25	3.860504529336939E-4	0.0	0.0	31.35250845933055	0.0
26	3.860504529336939E-4	0.0	0.0	32.18560533676146	0.0
27	3.860504529336939E-4	0.0	0.0	33.00345322130149	0.0
28	3.860504529336939E-4	0.0	0.0	33.90198565050466	0.0
29	3.860504529336939E-4	0.0	0.0	34.7978157265373	0.0
30	3.860504529336939E-4	0.0	0.0	35.770276817477274	0.0
31	3.860504529336939E-4	0.0	0.0	36.63696008431342	0.0
32	3.860504529336939E-4	0.0	0.0	37.46445923017679	0.0
33	3.860504529336939E-4	0.0	0.0	38.323035437501325	0.0
34	3.860504529336939E-4	0.0	0.0	39.14262054907956	0.0
35	3.860504529336939E-4	0.0	0.0	40.00332003389523	0.0
36	3.860504529336939E-4	0.0	0.0	40.784879175859494	0.0
37	3.860504529336939E-4	0.0	0.0	41.544433442006536	0.0
38	3.860504529336939E-4	0.0	0.0	42.313831994703385	0.0
39	3.860504529336939E-4	0.0	0.0	43.14017298920796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	35	1.2101918E-7	45.000004	1
ATACGTA	45	3.8380676E-10	45.000004	19
GAGTGCG	20	7.029696E-4	45.0	9
CGGCGCG	20	7.029696E-4	45.0	17
ACACGCG	20	7.029696E-4	45.0	36
GCGACAT	20	7.029696E-4	45.0	37
GGTGTCG	20	7.029696E-4	45.0	8
CACGGGA	25	3.8878294E-5	45.0	4
CACGGCG	20	7.029696E-4	45.0	15
CGCGGAA	20	7.029696E-4	45.0	4
GGCGTTA	20	7.029696E-4	45.0	8
GACGTAT	20	7.029696E-4	45.0	17
TAGCGCG	20	7.029696E-4	45.0	1
GCGCCCC	25	3.8878294E-5	45.0	9
GAATACG	20	7.029696E-4	45.0	15
TACGGGT	50	2.1827873E-11	45.0	4
CCGCTCG	20	7.029696E-4	45.0	1
ACGGGTA	40	6.8030204E-9	45.0	5
TCTAGCG	25	3.8878294E-5	45.0	1
CCCACTA	20	7.029696E-4	45.0	34
>>END_MODULE