##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933894.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935397
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33406991897558	33.0	31.0	34.0	31.0	34.0
2	32.53041970414701	34.0	31.0	34.0	31.0	34.0
3	32.605414599362625	34.0	31.0	34.0	31.0	34.0
4	36.024725330528106	37.0	35.0	37.0	35.0	37.0
5	36.046780137203775	37.0	35.0	37.0	35.0	37.0
6	35.42725281351127	37.0	35.0	37.0	33.0	37.0
7	35.89031715945208	37.0	35.0	37.0	35.0	37.0
8	35.82579589201163	37.0	35.0	37.0	35.0	37.0
9	37.720189395518695	39.0	38.0	39.0	35.0	39.0
10	37.51428537829392	39.0	37.0	39.0	35.0	39.0
11	37.50901809605975	39.0	37.0	39.0	35.0	39.0
12	37.352775345655374	39.0	37.0	39.0	35.0	39.0
13	37.26627303700995	39.0	37.0	39.0	34.0	39.0
14	38.51104290477733	40.0	38.0	41.0	34.0	41.0
15	38.501857500077506	40.0	38.0	41.0	34.0	41.0
16	38.64421523695287	40.0	38.0	41.0	35.0	41.0
17	38.531943121476765	40.0	38.0	41.0	34.0	41.0
18	38.49651752143742	40.0	38.0	41.0	34.0	41.0
19	38.51929501591303	40.0	38.0	41.0	34.0	41.0
20	38.39166471562342	40.0	38.0	41.0	34.0	41.0
21	38.41745376562037	40.0	38.0	41.0	34.0	41.0
22	38.4140445179961	40.0	38.0	41.0	34.0	41.0
23	38.315700178640725	40.0	37.0	41.0	34.0	41.0
24	38.30153613920079	40.0	37.0	41.0	34.0	41.0
25	38.220522409201656	40.0	37.0	41.0	34.0	41.0
26	38.13299807461431	40.0	37.0	41.0	34.0	41.0
27	38.0798441731158	40.0	37.0	41.0	34.0	41.0
28	38.00809709674074	40.0	37.0	41.0	34.0	41.0
29	38.01101885081949	40.0	37.0	41.0	34.0	41.0
30	37.99328306590677	40.0	37.0	41.0	34.0	41.0
31	37.912347377637516	40.0	37.0	41.0	34.0	41.0
32	37.775384141706674	40.0	37.0	41.0	33.0	41.0
33	37.67385933459269	40.0	36.0	41.0	33.0	41.0
34	37.59206304916522	40.0	36.0	41.0	33.0	41.0
35	37.599776351645346	40.0	36.0	41.0	33.0	41.0
36	37.36011875171719	40.0	36.0	41.0	32.0	41.0
37	37.361641099982144	40.0	36.0	41.0	33.0	41.0
38	37.290912842354636	40.0	36.0	41.0	32.0	41.0
39	37.322933471028875	40.0	36.0	41.0	33.0	41.0
40	37.283375935565324	40.0	36.0	41.0	33.0	41.0
41	37.045258858003606	39.0	35.0	41.0	31.0	41.0
42	37.074135367122196	39.0	35.0	41.0	32.0	41.0
43	36.85215475354315	39.0	35.0	41.0	31.0	41.0
44	36.906083727016444	39.0	35.0	41.0	31.0	41.0
45	36.968140800109474	39.0	35.0	41.0	32.0	41.0
46	36.894027883347924	39.0	35.0	41.0	31.0	41.0
47	36.72919626639812	39.0	35.0	41.0	31.0	41.0
48	36.71842650767535	39.0	35.0	41.0	31.0	41.0
49	36.784198580923395	39.0	35.0	41.0	31.0	41.0
50	36.652706818602155	39.0	35.0	41.0	31.0	41.0
51	35.786971734995944	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	3.0
9	10.0
10	16.0
11	16.0
12	10.0
13	9.0
14	13.0
15	13.0
16	37.0
17	57.0
18	118.0
19	309.0
20	658.0
21	1267.0
22	1936.0
23	2474.0
24	3168.0
25	4119.0
26	4938.0
27	5948.0
28	6741.0
29	8348.0
30	11064.0
31	14747.0
32	19850.0
33	28043.0
34	49491.0
35	68394.0
36	67156.0
37	106623.0
38	207263.0
39	322340.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.97444507519267	4.385838312502606	48.96338132365188	14.676335288652838
2	19.51086009469776	12.150242089722333	47.097756353719326	21.24114146186058
3	20.28518372412997	12.749773625530123	46.06161875652798	20.903423893811933
4	19.413575198552056	4.682076166590229	52.51748722734839	23.386861407509326
5	23.693576096566485	5.929140247402975	44.9884915175054	25.38879213852514
6	20.893695404197363	12.647677937816779	48.9441381573813	17.514488500604557
7	75.65258387615098	2.917905445495335	16.048586856703626	5.380923821650059
8	74.70849275762056	8.939092171559242	9.816687459976887	6.53572761084331
9	68.81719740388306	6.012206581804303	11.951930570656096	13.218665443656544
10	36.48589850085044	26.519648876359447	20.433569917371983	16.560882705418127
11	29.732616204670315	23.599605301278494	26.807868744500997	19.85990974955019
12	25.74030064240103	20.645779278744747	31.015921581959315	22.5979984968949
13	24.208651513742293	22.74905735211894	34.25753984671749	18.78475128742128
14	19.75385852210345	27.83844720476974	29.014953009257034	23.39274126386978
15	16.672493069787482	24.893815139454155	37.4134191150923	21.020272675666053
16	18.330826376394192	25.15028378324925	30.279122126754736	26.239767713601818
17	18.924371149362248	25.759864528109453	30.633303292612656	24.68246102991564
18	18.92340899104872	24.37243224000077	32.197986523369224	24.506172245581286
19	20.906631088190363	25.539423367832054	29.406337629904733	24.14760791407285
20	23.443414935048967	25.350305805983986	32.45317228941295	18.753106969554104
21	23.169948161048197	28.349460175732872	28.848927246933652	19.631664416285275
22	20.385034375778414	23.870613226255806	30.24512586634338	25.499226531622405
23	20.94308619762518	27.05974040968701	29.239456615747113	22.7577167769407
24	22.599922813521957	23.10345233093542	29.002231138222594	25.294393717320023
25	18.925654027113623	29.923337363707603	26.80872399633525	24.34228461284353
26	19.407802248670887	27.379497689216446	28.98031530996999	24.232384752142675
27	22.44362554081315	28.32027470688916	27.267352792450694	21.968746959846996
28	16.988080996624962	28.30477326739342	31.56905570575916	23.13809003022246
29	20.641503019573506	25.62847646507312	30.14559593413278	23.584424581220595
30	23.68341998103479	27.137568326603574	28.450379892174126	20.728631800187515
31	23.924494091813422	27.809689361843155	25.414449693552577	22.851366852790846
32	25.53450566978513	27.93156274822348	26.885162129021158	19.648769452970235
33	25.144297020409518	23.905144019063563	26.749818526251424	24.2007404342755
34	24.4858600145179	25.90055345484324	27.737206768890644	21.876379761748222
35	25.170061481916235	23.512262707705926	27.418625460633294	23.899050349744545
36	23.305826296214335	28.83791587956771	27.531091076836894	20.32516674738106
37	23.770548761648797	25.314919761341976	31.258813102885725	19.655718374123502
38	22.643861376506447	26.91702025984689	27.503295392223837	22.93582297142283
39	24.662469518290095	23.66599422491199	31.254109217797364	20.41742703900055
40	28.30295585724564	22.25782207982279	28.68653630490583	20.752685758025738
41	24.298559862817605	24.363345189261885	27.88944159538677	23.44865335253374
42	24.377136125089134	23.09564815794791	29.988657222548287	22.53855849441467
43	23.49964774315077	23.61970371938332	29.838346712679215	23.042301824786694
44	22.837682823442883	23.07426686209171	29.31300827349243	24.775042040972977
45	22.60323691437967	22.56047432266727	27.322409629280404	27.513879133672653
46	26.310432896406553	24.734524485325483	27.982770951799075	20.972271666468888
47	20.64620690466187	23.52113594548625	34.22910272322875	21.603554426623134
48	22.267978195354484	23.35350658597366	29.92761362287884	24.450901595793017
49	22.34815805481523	20.708426475603407	34.50577669160795	22.43763877797342
50	23.908885745838397	20.830086049025173	30.11106514132502	25.14996306381141
51	21.990128255703194	21.05202390001251	27.308725599932437	29.64912224435186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	484.5
2	848.0
3	1797.5
4	2747.0
5	1940.0
6	1133.0
7	1162.5
8	1192.0
9	1327.0
10	1462.0
11	1471.5
12	1481.0
13	1458.0
14	1435.0
15	1450.0
16	1465.0
17	1421.5
18	1378.0
19	1604.0
20	1830.0
21	2293.0
22	2756.0
23	3280.0
24	3804.0
25	4400.0
26	6315.5
27	7635.0
28	9149.0
29	10663.0
30	12716.5
31	14770.0
32	16657.0
33	18544.0
34	20882.5
35	23221.0
36	26249.5
37	29278.0
38	32354.0
39	35430.0
40	38724.5
41	42019.0
42	47798.0
43	53577.0
44	59073.0
45	64569.0
46	100293.0
47	136017.0
48	124403.0
49	112789.0
50	106850.0
51	100911.0
52	83695.0
53	66479.0
54	56402.5
55	46326.0
56	41472.0
57	36618.0
58	33507.0
59	30396.0
60	28310.5
61	26225.0
62	22443.5
63	18662.0
64	15660.5
65	12659.0
66	10660.0
67	8661.0
68	7122.5
69	5584.0
70	4390.5
71	3197.0
72	2679.5
73	2162.0
74	1682.5
75	910.5
76	618.0
77	479.0
78	340.0
79	210.0
80	80.0
81	71.0
82	62.0
83	46.5
84	31.0
85	23.0
86	15.0
87	9.5
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	935397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.95215206290054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06555827671829	19.30530987259949
2	12.699937141244977	7.099811483227161
3	5.221360825907401	4.378448153431068
4	2.651044371530149	2.9640958159402935
5	1.477592187709724	2.0650940758908045
6	0.9761692315590778	1.6371618479798469
7	0.6854795229655778	1.341243950335685
8	0.5559237095033737	1.243141125072804
9	0.43251673689446624	1.0880796239481298
>10	5.7718141331623976	39.304762566393705
>50	0.38576065370364493	6.520866052285069
>100	0.06603109927769668	3.2312900598282237
>500	0.004247614573419073	0.840176431362623
>1k	0.0046337613528208066	3.240788457305082
>5k	7.722935588034675E-4	1.308188379768113
>10k+	0.0011584403382052016	4.431542104631965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	14639	1.5650039501944095	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	13425	1.4352194843472879	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	12993	1.3890358852979001	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	6650	0.7109280872185821	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	5470	0.5847784416670141	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	4673	0.49957397768006523	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3563	0.38090778567816663	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	3279	0.3505463455623655	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	3255	0.34798059005962173	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	3072	0.32841670435120063	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	3008	0.3215746896772173	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	2624	0.2805226016333172	No Hit
TCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	1636	0.1748990001036993	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.1527693588925344	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	1252	0.1338469120597992	No Hit
GCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	1220	0.13042590472280752	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGT	1014	0.10840316999092363	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0690647928098978E-4	0.0	0.0	0.5223450577669161	0.0
2	1.0690647928098978E-4	0.0	0.0	2.3268195215507426	0.0
3	1.0690647928098978E-4	0.0	0.0	3.1982142341700905	0.0
4	1.0690647928098978E-4	0.0	0.0	4.363708671291441	0.0
5	1.0690647928098978E-4	0.0	0.0	7.197478717592637	0.0
6	1.0690647928098978E-4	0.0	0.0	8.093889546363737	0.0
7	1.0690647928098978E-4	0.0	0.0	9.209672470619427	0.0
8	1.0690647928098978E-4	0.0	0.0	10.643502170736062	0.0
9	1.0690647928098978E-4	0.0	0.0	11.14874219181802	0.0
10	1.0690647928098978E-4	0.0	0.0	13.719949925005105	0.0
11	1.0690647928098978E-4	0.0	0.0	15.61059101108941	0.0
12	1.0690647928098978E-4	0.0	0.0	19.009361800390636	0.0
13	1.0690647928098978E-4	0.0	0.0	19.687469598469953	0.0
14	1.0690647928098978E-4	0.0	0.0	20.013961986194097	0.0
15	1.0690647928098978E-4	0.0	0.0	20.69249741019054	0.0
16	1.0690647928098978E-4	0.0	0.0	21.634129679697498	0.0
17	1.0690647928098978E-4	0.0	0.0	22.699987278128965	0.0
18	1.0690647928098978E-4	0.0	0.0	23.76776919318749	0.0
19	1.0690647928098978E-4	0.0	0.0	24.98842737361783	0.0
20	2.1381295856197957E-4	0.0	0.0	25.84891762535052	0.0
21	2.1381295856197957E-4	0.0	0.0	26.999872781289657	0.0
22	2.1381295856197957E-4	0.0	0.0	28.21336822760817	0.0
23	2.1381295856197957E-4	0.0	0.0	29.27527028630624	0.0
24	2.1381295856197957E-4	0.0	0.0	30.13565363155965	0.0
25	2.1381295856197957E-4	0.0	0.0	30.873308338598477	0.0
26	2.1381295856197957E-4	0.0	0.0	31.559647935582433	0.0
27	2.1381295856197957E-4	0.0	0.0	32.24021458268521	0.0
28	2.1381295856197957E-4	0.0	0.0	32.909342236504926	0.0
29	2.1381295856197957E-4	0.0	0.0	33.61738384878292	0.0
30	2.1381295856197957E-4	0.0	0.0	34.40004618359905	0.0
31	3.2071943784296936E-4	0.0	0.0	35.15170563942369	0.0
32	3.2071943784296936E-4	0.0	0.0	35.84371127980953	0.0
33	3.2071943784296936E-4	0.0	0.0	36.51722209927977	0.0
34	3.2071943784296936E-4	0.0	0.0	37.218742416321625	0.0
35	3.2071943784296936E-4	0.0	0.0	37.91801769729858	0.0
36	4.2762591712395913E-4	0.0	0.0	38.57613398375236	0.0
37	5.345323964049489E-4	0.0	0.0	39.237243651625995	0.0
38	6.414388756859387E-4	0.0	0.0	39.89097677242925	0.0
39	6.414388756859387E-4	0.0	0.0	40.57004672882209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	60	0.0	45.000004	40
CGTCGGA	30	2.1650667E-6	45.000004	23
TTACGAG	30	2.1650667E-6	45.000004	1
TCGCCTA	20	7.032612E-4	45.0	13
ATTACGC	25	3.890247E-5	45.0	41
CGTCTAC	25	3.890247E-5	45.0	42
GATCACG	25	3.890247E-5	45.0	38
CCGCTAT	20	7.032612E-4	45.0	23
TACGCTA	20	7.032612E-4	45.0	28
CCATACG	20	7.032612E-4	45.0	2
TGTCGAT	20	7.032612E-4	45.0	9
TAGTCCG	20	7.032612E-4	45.0	33
ACGACTA	20	7.032612E-4	45.0	28
GTCGGTA	20	7.032612E-4	45.0	14
TAATCGA	20	7.032612E-4	45.0	28
TTGCCCG	85	0.0	42.35294	1
TGCTACG	240	0.0	42.187504	4
TATTGCG	80	0.0	42.1875	1
CGGGCTA	65	0.0	41.53846	6
TACGGCT	2010	0.0	41.41791	7
>>END_MODULE