##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933893.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99388
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.405833702257816	33.0	31.0	34.0	31.0	34.0
2	32.64258260554594	34.0	31.0	34.0	31.0	34.0
3	32.68892622851853	34.0	31.0	34.0	31.0	34.0
4	36.067543365396226	37.0	35.0	37.0	35.0	37.0
5	36.09693323137602	37.0	35.0	37.0	35.0	37.0
6	35.759779852698514	37.0	35.0	37.0	35.0	37.0
7	36.02869561717712	37.0	35.0	37.0	35.0	37.0
8	36.03323338833662	37.0	36.0	37.0	35.0	37.0
9	37.87884855314525	39.0	38.0	39.0	35.0	39.0
10	37.57268483116674	39.0	37.0	39.0	35.0	39.0
11	37.51612870769107	39.0	37.0	39.0	35.0	39.0
12	37.445325391395336	39.0	37.0	39.0	35.0	39.0
13	37.420080895077874	39.0	37.0	39.0	35.0	39.0
14	38.6818227552622	40.0	38.0	41.0	35.0	41.0
15	38.71197730108263	40.0	38.0	41.0	35.0	41.0
16	38.7684328087898	40.0	38.0	41.0	35.0	41.0
17	38.73897251177205	40.0	38.0	41.0	35.0	41.0
18	38.674055217933756	40.0	38.0	41.0	35.0	41.0
19	38.67812009498129	40.0	38.0	41.0	35.0	41.0
20	38.625659033283696	40.0	38.0	41.0	35.0	41.0
21	38.55191773654767	40.0	38.0	41.0	34.0	41.0
22	38.54483438644505	40.0	38.0	41.0	34.0	41.0
23	38.47458445687608	40.0	38.0	41.0	34.0	41.0
24	38.41386284058438	40.0	38.0	41.0	34.0	41.0
25	38.33484927757878	40.0	37.0	41.0	34.0	41.0
26	38.25246508632833	40.0	37.0	41.0	34.0	41.0
27	38.2515796675655	40.0	37.0	41.0	34.0	41.0
28	38.171308407453616	40.0	37.0	41.0	34.0	41.0
29	38.174417434700366	40.0	37.0	41.0	34.0	41.0
30	38.068841308809915	40.0	37.0	41.0	34.0	41.0
31	37.97808588562	40.0	37.0	41.0	34.0	41.0
32	37.87263049865175	40.0	37.0	41.0	33.0	41.0
33	37.76427737755061	40.0	37.0	41.0	33.0	41.0
34	37.71641445647362	40.0	37.0	41.0	33.0	41.0
35	37.68980158570451	40.0	37.0	41.0	33.0	41.0
36	37.58046243007204	40.0	37.0	41.0	33.0	41.0
37	37.48884171127299	40.0	36.0	41.0	33.0	41.0
38	37.44688493580714	40.0	36.0	41.0	33.0	41.0
39	37.37027608966877	40.0	36.0	41.0	33.0	41.0
40	37.219714653680526	40.0	36.0	41.0	32.0	41.0
41	37.110405682778605	40.0	35.0	41.0	32.0	41.0
42	37.11709663138407	40.0	35.0	41.0	32.0	41.0
43	37.066366160904735	39.0	35.0	41.0	32.0	41.0
44	36.94999396305389	39.0	35.0	41.0	31.0	41.0
45	36.94287036664386	39.0	35.0	41.0	31.0	41.0
46	36.81765404274158	39.0	35.0	41.0	31.0	41.0
47	36.74577413772286	39.0	35.0	41.0	31.0	41.0
48	36.70833501026281	39.0	35.0	41.0	31.0	41.0
49	36.7111321286272	39.0	35.0	41.0	31.0	41.0
50	36.61165331830804	39.0	35.0	41.0	31.0	41.0
51	35.69591902442951	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	3.0
13	3.0
14	4.0
15	5.0
16	3.0
17	3.0
18	13.0
19	28.0
20	54.0
21	83.0
22	144.0
23	192.0
24	290.0
25	407.0
26	618.0
27	793.0
28	898.0
29	964.0
30	1154.0
31	1456.0
32	1968.0
33	2698.0
34	4653.0
35	6238.0
36	7042.0
37	11310.0
38	21691.0
39	36647.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.99975852215559	4.715861069746851	56.637622248158735	15.646758159938825
2	21.121262124200104	4.526703424960759	52.33730430233025	22.014730148508875
3	20.4441180021733	4.288244053608081	52.84239546021653	22.42524248400209
4	18.499215197005675	4.823519942045317	51.42673159737594	25.250533263573065
5	17.97299472773373	5.655612347567111	51.15104439167706	25.220348533022097
6	21.30840745361613	6.116432567311949	53.304221837646395	19.270938141425525
7	81.21201754739002	2.0827464080170643	11.450074455668693	5.255161588924216
8	82.45462228840505	2.842395460216525	8.75558417515193	5.947398076226506
9	76.57866140781583	5.76025274681048	11.664386042580594	5.996699802793094
10	33.912544774016986	35.12898941522115	17.306918340242284	13.65154747051958
11	23.91838048859017	26.12689660723629	30.132410351350263	19.82231255282328
12	23.387129230893066	22.752243731637623	33.88839698957621	19.972230047893106
13	22.496679679639392	24.171932225218338	33.431601400571495	19.89978669457077
14	19.60498249285628	26.1118042419608	32.00990059162072	22.273312673562202
15	17.560470076870445	27.655250130800503	33.29577011309212	21.48850967923693
16	20.12013522759287	27.921881917334083	29.920111079808425	22.03787177526462
17	20.562844609007126	27.405723024912465	28.807300680162594	23.22413168591782
18	20.337465287559866	26.459934800982012	30.722018754779253	22.480581156678873
19	21.444238741095504	27.99231295528635	29.33754578017467	21.225902523443473
20	23.333802873586347	28.280074053205617	29.499537167464883	18.886585905743146
21	24.247394051595766	27.05155551978106	29.561918943936895	19.13913148468628
22	22.447377953072806	25.74355052923894	29.07996941280637	22.729102104881875
23	21.872861914919305	26.42773775506097	28.757998953596008	22.941401376423713
24	22.266269569766976	26.188272226023262	28.984384432728298	22.561073771481468
25	20.47631504809434	27.45502475147905	26.996216847104275	25.07244335332233
26	20.412927113937297	28.38169597939389	27.13506660763875	24.070310299030066
27	20.331428341449673	26.907674970821425	29.702781019841428	23.05811566788747
28	18.138004588079042	26.223487744999396	31.127500301847306	24.511007365074256
29	19.323258341047207	24.384231496760172	29.822513784360282	26.469996377832334
30	22.10025355173663	25.57552219583853	29.543808105606313	22.78041614681853
31	21.14339759327082	28.200587596088063	27.94904817482996	22.706966635811167
32	21.356703022497687	27.49124642814022	27.172294441984945	23.97975610737715
33	21.851732603533623	26.564575200225377	27.253793214472573	24.329898981768423
34	19.685475107658874	26.065520988449308	29.51966032116553	24.729343582726283
35	19.50134825129794	24.79876846299352	28.141224292671147	27.558658993037387
36	20.188553950175073	26.311023463597216	29.049784682255403	24.45063790397231
37	19.457077313156518	27.10790035014287	30.95745965307683	22.477562683623777
38	20.386767014126452	27.019358473860024	28.56179820501469	24.032076306998835
39	22.135469070712762	25.770716786734816	27.818247675775748	24.275566466776674
40	22.596289290457598	26.742665110476118	28.337425041252466	22.32362055781382
41	18.81917334084598	26.96804443192337	28.96325512134262	25.249527105888035
42	20.17144926952952	25.418561596973476	28.349498933472855	26.06049020002415
43	20.880790437477362	24.079365718195355	29.483438644504368	25.55640519982292
44	20.400853221716908	24.93258743510283	27.729705799492898	26.936853543687366
45	21.5931500784803	23.982774580432245	26.809071517688253	27.615003823399203
46	21.697790477723668	25.24751479051797	29.27818247675776	23.776512255000604
47	18.35332233267598	25.988046846701817	31.237171489515838	24.42145933110637
48	18.386525536282047	24.497927315168834	31.413249084396504	25.70229806415261
49	20.61516480862881	23.41530164607397	31.478649333923613	24.490884211373608
50	20.28112045719805	23.693001167142917	29.831569203525575	26.194309172133458
51	19.166297742182152	23.061134140942567	28.415905340684994	29.356662776190284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	40.0
2	70.0
3	298.5
4	527.0
5	370.5
6	214.0
7	242.5
8	271.0
9	273.0
10	275.0
11	334.5
12	394.0
13	383.0
14	372.0
15	386.5
16	401.0
17	388.0
18	375.0
19	389.0
20	403.0
21	413.0
22	423.0
23	476.0
24	529.0
25	588.0
26	795.0
27	943.0
28	1104.5
29	1266.0
30	1460.0
31	1654.0
32	1861.5
33	2069.0
34	2411.5
35	2754.0
36	3030.5
37	3307.0
38	3543.5
39	3780.0
40	4316.0
41	4852.0
42	5378.5
43	5905.0
44	6627.0
45	7349.0
46	8610.5
47	9872.0
48	10921.5
49	11971.0
50	11316.0
51	10661.0
52	8777.0
53	6893.0
54	5984.5
55	5076.0
56	4661.0
57	4246.0
58	3765.5
59	3285.0
60	3071.0
61	2857.0
62	2450.0
63	2043.0
64	1746.0
65	1449.0
66	1165.5
67	882.0
68	697.5
69	513.0
70	425.0
71	337.0
72	283.5
73	230.0
74	191.0
75	111.5
76	71.0
77	53.0
78	35.0
79	20.5
80	6.0
81	11.0
82	16.0
83	9.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	99388.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7734132893307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08343041769577	49.92956896204773
2	12.305637106419995	15.94156236165332
3	5.487984839305963	10.66426530365839
4	2.5878807648694413	6.705034813055902
5	1.205399443900772	3.903891817925705
6	0.49241188623266074	1.9137119169316215
7	0.2594094164064806	1.1761983338028736
8	0.12116128430961369	0.6278423954602166
9	0.08232753933858365	0.479937215760454
>10	0.3308635071531758	3.6644262888879946
>50	0.021746897183776814	0.9407574355052924
>100	0.017086847787253213	2.379562925101622
>500	0.004660049396523603	1.6732402302088785
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	584	0.5875960880589206	RNA PCR Primer, Index 21 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	571	0.5745160381534995	TruSeq Adapter, Index 21 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	508	0.5111281039964584	RNA PCR Primer, Index 21 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	491	0.4940234233509076	TruSeq Adapter, Index 21 (96% over 25bp)
CGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	352	0.35416750513140416	RNA PCR Primer, Index 21 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.31492735541514066	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	235	0.2364470559826136	RNA PCR Primer, Index 21 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	193	0.19418843321125287	RNA PCR Primer, Index 21 (96% over 25bp)
TTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	161	0.16199138729021614	TruSeq Adapter, Index 21 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	146	0.14689902201473015	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	135	0.13583128747937376	RNA PCR Primer, Index 14 (95% over 22bp)
CGGTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	118	0.118726606833823	RNA PCR Primer, Index 21 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	111	0.1116835030385962	TruSeq Adapter, Index 21 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	110	0.11067734535356383	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	100	0.10061576850323983	RNA PCR Primer, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.618786976294925	0.0
2	0.0	0.0	0.0	2.3201996216847105	0.0
3	0.0	0.0	0.0	3.3615728256932425	0.0
4	0.0	0.0	0.0	4.884895560832294	0.0
5	0.0	0.0	0.0	6.756348854992554	0.0
6	0.0	0.0	0.0	7.923491769630137	0.0
7	0.0	0.0	0.0	8.850162997544976	0.0
8	0.0	0.0	0.0	10.213506660763874	0.0
9	0.0	0.0	0.0	10.872539944460096	0.0
10	0.0	0.0	0.0	11.984344186420897	0.0
11	0.0	0.0	0.0	14.040930494627117	0.0
12	0.0	0.0	0.0	15.809755704914075	0.0
13	0.0	0.0	0.0	16.431561154264095	0.0
14	0.0	0.0	0.0	16.688131363947356	0.0
15	0.0	0.0	0.0	17.02519418843321	0.0
16	0.0	0.0	0.0	17.694289048979755	0.0
17	0.0	0.0	0.0	18.713526783917576	0.0
18	0.0	0.0	0.0	19.90783595605103	0.0
19	0.0	0.0	0.0	20.56485692437719	0.0
20	0.0	0.0	0.0	21.259105727049544	0.0
21	0.0	0.0	0.0	22.11333360164205	0.0
22	0.0	0.0	0.0	22.974604580029784	0.0
23	0.0	0.0	0.0	23.802672354811445	0.0
24	0.0	0.0	0.0	24.451644061657344	0.0
25	0.0	0.0	0.0	25.018110838330582	0.0
26	0.0	0.0	0.0	25.509115788626392	0.0
27	0.0	0.0	0.0	25.993077635126976	0.0
28	0.0	0.0	0.0	26.48106411236769	0.0
29	0.0	0.0	0.0	27.024389262285187	0.0
30	0.0	0.0	0.0	27.657262446170563	0.0
31	0.0	0.0	0.0	28.148267396466373	0.0
32	0.0	0.0	0.0	28.606069143156116	0.0
33	0.0	0.0	0.0	29.075944782066244	0.0
34	0.0	0.0	0.0	29.587072886062703	0.0
35	0.0	0.0	0.0	30.132410351350263	0.0
36	0.0	0.0	0.0	30.63850766692156	0.0
37	0.0	0.0	0.0	31.12448182879221	0.0
38	0.0	0.0	0.0	31.58127741779692	0.0
39	0.0	0.0	0.0	32.10045478327363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCGG	30	2.145467E-6	45.000004	2
TACGGGA	30	2.145467E-6	45.000004	4
CGGGAAC	60	0.0	45.000004	6
TTTGCCG	30	2.145467E-6	45.000004	1
GGAGACC	20	7.0021796E-4	45.0	8
TTGCTCG	20	7.0021796E-4	45.0	1
TTTCGCG	20	7.0021796E-4	45.0	1
CAACAAT	20	7.0021796E-4	45.0	26
TGACGGG	20	7.0021796E-4	45.0	3
ACGGGAT	20	7.0021796E-4	45.0	5
CGGGATA	45	3.765308E-10	45.0	6
GCGATTC	35	1.1970042E-7	45.0	9
CGGGACT	40	6.706614E-9	45.0	6
GACGGGA	20	7.0021796E-4	45.0	4
GCGATGC	20	7.0021796E-4	45.0	9
ACGTCTC	20	7.0021796E-4	45.0	34
TCTTTCG	25	3.865044E-5	45.0	1
TTGCCGG	35	1.1970042E-7	45.0	2
GAATTGA	20	7.0021796E-4	45.0	9
GGCGATG	40	6.706614E-9	45.0	8
>>END_MODULE