##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933891.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391690
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45611325282749	33.0	31.0	34.0	31.0	34.0
2	32.68837345860246	34.0	31.0	34.0	31.0	34.0
3	32.744272256120915	34.0	31.0	34.0	31.0	34.0
4	36.10855523500727	37.0	35.0	37.0	35.0	37.0
5	36.14463478771477	37.0	35.0	37.0	35.0	37.0
6	35.75934029462075	37.0	35.0	37.0	35.0	37.0
7	36.07020347723965	37.0	35.0	37.0	35.0	37.0
8	36.07350711021471	37.0	36.0	37.0	35.0	37.0
9	37.91886185503842	39.0	38.0	39.0	35.0	39.0
10	37.661219331614284	39.0	37.0	39.0	35.0	39.0
11	37.625933774158135	39.0	37.0	39.0	35.0	39.0
12	37.377883019734995	39.0	37.0	39.0	35.0	39.0
13	37.25032806556205	39.0	37.0	39.0	34.0	39.0
14	38.483047818427835	40.0	38.0	41.0	34.0	41.0
15	38.56672113150706	40.0	38.0	41.0	35.0	41.0
16	38.64144348847303	40.0	38.0	41.0	35.0	41.0
17	38.6471367663203	40.0	38.0	41.0	35.0	41.0
18	38.57937654778013	40.0	38.0	41.0	35.0	41.0
19	38.59701549694912	40.0	38.0	41.0	35.0	41.0
20	38.54291914524241	40.0	37.0	41.0	35.0	41.0
21	38.45440271643391	40.0	37.0	41.0	34.0	41.0
22	38.44430033955424	40.0	37.0	41.0	35.0	41.0
23	38.39615001659475	40.0	37.0	41.0	34.0	41.0
24	38.38301718195512	40.0	37.0	41.0	34.0	41.0
25	38.29818989507008	40.0	37.0	41.0	34.0	41.0
26	38.204332507850594	40.0	37.0	41.0	34.0	41.0
27	38.188439837626696	40.0	37.0	41.0	34.0	41.0
28	38.144057800811865	40.0	37.0	41.0	34.0	41.0
29	38.134621767213865	40.0	37.0	41.0	34.0	41.0
30	38.082422323776456	40.0	36.0	41.0	34.0	41.0
31	38.02856085169394	40.0	36.0	41.0	34.0	41.0
32	37.91431233883939	40.0	36.0	41.0	34.0	41.0
33	37.839932089152136	40.0	36.0	41.0	34.0	41.0
34	37.84211493783349	40.0	36.0	41.0	34.0	41.0
35	37.76670836631009	40.0	36.0	41.0	34.0	41.0
36	37.65763231126656	40.0	36.0	41.0	33.0	41.0
37	37.593849728101304	40.0	36.0	41.0	33.0	41.0
38	37.53470601751385	40.0	35.0	41.0	33.0	41.0
39	37.51757257014476	40.0	35.0	41.0	33.0	41.0
40	37.40691107763793	40.0	35.0	41.0	33.0	41.0
41	37.304807373177766	39.0	35.0	41.0	33.0	41.0
42	37.27288161556333	39.0	35.0	41.0	33.0	41.0
43	37.12392197911614	39.0	35.0	41.0	33.0	41.0
44	37.015461206566414	39.0	35.0	41.0	32.0	41.0
45	37.032890806505144	39.0	35.0	41.0	32.0	41.0
46	36.98187086726748	39.0	35.0	41.0	32.0	41.0
47	36.92045239858051	39.0	35.0	41.0	32.0	41.0
48	36.86327963440476	39.0	35.0	41.0	32.0	41.0
49	36.8700681661518	39.0	35.0	41.0	32.0	41.0
50	36.77073450943348	39.0	35.0	41.0	32.0	41.0
51	35.80015062932421	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	15.0
11	13.0
12	6.0
13	11.0
14	8.0
15	17.0
16	12.0
17	33.0
18	47.0
19	111.0
20	168.0
21	271.0
22	440.0
23	595.0
24	902.0
25	1339.0
26	1835.0
27	2288.0
28	2599.0
29	3263.0
30	3990.0
31	5319.0
32	7291.0
33	10646.0
34	21908.0
35	35277.0
36	25830.0
37	41555.0
38	84130.0
39	141655.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.286323367969565	5.395848757946335	53.64216599862136	15.675661875462737
2	20.690086548035435	5.703234700911435	52.981694707549345	20.62498404350379
3	20.821057468916745	5.135694043759095	52.22956930225433	21.813679185069827
4	19.77252419004825	5.1936480379892265	50.803186193162965	24.23064157879956
5	18.803135132375093	6.364471903801476	49.840179733973294	24.992213229850137
6	21.91707728050244	7.260078123005438	52.44785417038985	18.374990426102276
7	82.65439505731574	2.8788072199954042	9.52743240828206	4.939365314406801
8	83.84079246342772	3.7805407337435217	7.181955117567464	5.196711685261303
9	77.55444356506422	6.362429472286757	9.818223595190073	6.26490336745896
10	38.161811636753555	29.955577114554877	17.24322806300901	14.639383185682556
11	31.720237943271464	24.389695933008245	24.410630856034107	19.479435267686178
12	28.647655033317164	21.220863437922848	29.513645995557713	20.61783553320228
13	25.03867854680998	25.113737904975874	29.902473895172204	19.945109653041946
14	20.32015113993209	28.155429038270057	30.043656973627105	21.480762848170748
15	17.858255252878553	25.14182133830325	35.69991574970002	21.30000765911818
16	19.499859582833363	24.22042942122597	33.387117363220916	22.892593632719752
17	20.460568306568973	23.9761035512778	28.444688401542034	27.1186397406112
18	21.084020526436724	24.32510403635528	31.722280374786184	22.86859506242181
19	22.855319257576145	25.679491434552837	29.072225484439222	22.392963823431796
20	25.70017105363936	25.595496438510047	29.09698996655518	19.60734254129541
21	24.024100691873677	26.167121958691826	29.23127983864791	20.577497510786593
22	22.891827720901734	24.39786565906712	28.277719625213816	24.432586994817328
23	21.73759861114657	25.010850417421942	27.69077586867165	25.560775102759838
24	22.689627000944622	23.30848374990426	30.024253874237278	23.977635374913834
25	19.48913681738109	26.056830656897034	29.866731343664632	24.58730118205724
26	20.246112997523554	29.336465061655904	27.47019326508208	22.94722867573847
27	21.35566391789425	28.63591105210753	28.038499834052438	21.969925195945773
28	19.205238836835253	26.816359876432895	31.626796701473104	22.351604585258748
29	20.215476524802778	26.36038704077204	30.065868416349666	23.358268018075517
30	21.3694503306186	26.498761775894202	29.577982588271333	22.55380530521586
31	23.80887947101024	27.747453343205088	25.163777477086473	23.279889708698207
32	23.903597232505298	28.2544869667339	25.845183691184353	21.99673210957645
33	23.65135694043759	25.912583931170058	27.57614439990809	22.85991472848426
34	21.60739360208328	25.32130000765912	28.21210651280349	24.85919987745411
35	21.52671755725191	26.50208072710562	27.50287216931757	24.4683295463249
36	24.80175649110266	25.255176287370112	28.422987566698154	21.520079654829075
37	23.867854680997727	26.56258776072915	27.923357757410194	21.646199800862927
38	21.984732824427482	27.869743930148843	27.1398299675764	23.00569327784728
39	21.999795756848528	25.893691439658912	29.601470550690596	22.50504225280196
40	22.93165513543874	25.086930991345195	28.257805917945312	23.72360795527075
41	20.527713242615334	25.132630396487016	27.74132604866093	26.59833031223672
42	23.57093619954556	23.980188414307232	28.064030227986418	24.38484515816079
43	24.023845387934337	24.48186065510991	27.880977303479792	23.613316653475962
44	21.543312313308995	25.30036508463326	28.171002578569787	24.98532002348796
45	21.044193111899716	26.15486736960351	26.330771783808625	26.470167734688143
46	23.066966223288826	25.374147923102452	28.325972069749035	23.232913783859686
47	21.059766652199443	25.01978605529883	29.663764711889506	24.256682580612217
48	21.72381219842222	23.641144782864	30.35410656386428	24.280936454849495
49	21.778447241440936	22.520360489162346	32.38173045010085	23.319461819295874
50	21.92652352625801	23.243636549311955	29.84349868518471	24.98634123924532
51	20.540989047461004	23.663866833465242	27.40764379994383	28.387500319129927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	246.0
2	333.0
3	744.0
4	1155.0
5	808.5
6	462.0
7	495.5
8	529.0
9	596.0
10	663.0
11	674.5
12	686.0
13	719.5
14	753.0
15	708.0
16	663.0
17	687.0
18	711.0
19	795.0
20	879.0
21	823.5
22	768.0
23	1202.5
24	1637.0
25	1902.5
26	2928.0
27	3688.0
28	3770.0
29	3852.0
30	4777.5
31	5703.0
32	6779.5
33	7856.0
34	9101.0
35	10346.0
36	11139.5
37	11933.0
38	12696.0
39	13459.0
40	17157.5
41	20856.0
42	22589.0
43	24322.0
44	27482.5
45	30643.0
46	34571.5
47	38500.0
48	41974.5
49	45449.0
50	43925.5
51	42402.0
52	35577.0
53	28752.0
54	25213.0
55	21674.0
56	19520.5
57	17367.0
58	15968.0
59	14569.0
60	12821.5
61	11074.0
62	10297.5
63	9521.0
64	8151.5
65	6782.0
66	5828.5
67	4875.0
68	3839.5
69	2804.0
70	2293.5
71	1783.0
72	1356.0
73	929.0
74	665.0
75	316.5
76	232.0
77	207.0
78	182.0
79	145.5
80	109.0
81	80.5
82	52.0
83	27.5
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.976018925424846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.41530372303923	22.121617996898266
2	12.067605824391332	7.47612772801826
3	4.553334170757969	4.231324963415475
4	2.279472613209486	2.824359472270587
5	1.2903505995946913	1.9984962296739226
6	0.8694025794600605	1.6158378453100808
7	0.6496994255211725	1.4087571190547044
8	0.44742907989808023	1.1087657317366686
9	0.3673515930493497	1.0241180908723462
>10	5.546584755663414	43.09776495555438
>50	0.46393518826905605	8.625843324676758
>100	0.04540290988398235	2.6284363541443803
>500	8.255074524360427E-4	0.15342560681842868
>1k	0.0033020298097441707	1.6851245815557419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	2180	0.5565625877607292	No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT	1712	0.4370803441497102	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1477	0.377083918404861	No Hit
TCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1221	0.3117261099338763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	600	0.1531823636038704	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTG	464	0.11846102785365979	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTG	440	0.11233373330950497	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	434	0.11080190967346626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC	420	0.10722765452270928	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG	407	0.1039087033112921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5530393933978403E-4	0.0	0.0	0.45469631596415533	0.0
2	2.5530393933978403E-4	0.0	0.0	1.7912124384079247	0.0
3	2.5530393933978403E-4	0.0	0.0	2.502233909469223	0.0
4	2.5530393933978403E-4	0.0	0.0	3.2523168832495086	0.0
5	2.5530393933978403E-4	0.0	0.0	4.59827925144885	0.0
6	2.5530393933978403E-4	0.0	0.0	5.325640174627894	0.0
7	2.5530393933978403E-4	0.0	0.0	6.024662360540223	0.0
8	2.5530393933978403E-4	0.0	0.0	7.122469299701295	0.0
9	2.5530393933978403E-4	0.0	0.0	7.6652454747376755	0.0
10	2.5530393933978403E-4	0.0	0.0	8.808241211161889	0.0
11	2.5530393933978403E-4	0.0	0.0	10.630600730169267	0.0
12	2.5530393933978403E-4	0.0	0.0	12.444790523117772	0.0
13	2.5530393933978403E-4	0.0	0.0	13.034287319053332	0.0
14	2.5530393933978403E-4	0.0	0.0	13.227552401133549	0.0
15	2.5530393933978403E-4	0.0	0.0	13.579871837422452	0.0
16	2.5530393933978403E-4	0.0	0.0	14.331997242717454	0.0
17	2.5530393933978403E-4	0.0	0.0	15.444611810360234	0.0
18	2.5530393933978403E-4	0.0	0.0	16.686154867369602	0.0
19	2.5530393933978403E-4	0.0	0.0	17.48117133447369	0.0
20	2.5530393933978403E-4	0.0	0.0	18.330822844596494	0.0
21	2.5530393933978403E-4	0.0	0.0	19.414843371033214	0.0
22	2.5530393933978403E-4	0.0	0.0	20.54379739079374	0.0
23	2.5530393933978403E-4	0.0	0.0	21.58160790420996	0.0
24	2.5530393933978403E-4	0.0	0.0	22.40138885343001	0.0
25	2.5530393933978403E-4	0.0	0.0	23.09938982358498	0.0
26	2.5530393933978403E-4	0.0	0.0	23.760627026475017	0.0
27	2.5530393933978403E-4	0.0	0.0	24.378717863616636	0.0
28	2.5530393933978403E-4	0.0	0.0	25.03663611529526	0.0
29	2.5530393933978403E-4	0.0	0.0	25.700936965457377	0.0
30	2.5530393933978403E-4	0.0	0.0	26.47680563711098	0.0
31	2.5530393933978403E-4	0.0	0.0	27.171487656054534	0.0
32	2.5530393933978403E-4	0.0	0.0	27.86157420408997	0.0
33	2.5530393933978403E-4	0.0	0.0	28.49855753274273	0.0
34	2.5530393933978403E-4	0.0	0.0	29.10184074140264	0.0
35	5.106078786795681E-4	0.0	0.0	29.783246955500523	0.0
36	5.106078786795681E-4	0.0	0.0	30.423549235364703	0.0
37	5.106078786795681E-4	0.0	0.0	31.06563864280426	0.0
38	5.106078786795681E-4	0.0	0.0	31.71436595266665	0.0
39	5.106078786795681E-4	0.0	0.0	32.330414358293545	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	40	6.7957444E-9	45.0	3
CGAAAGA	20	7.027586E-4	45.0	31
AACCCTC	20	7.027586E-4	45.0	21
ACCATAA	20	7.027586E-4	45.0	45
CGTTGAA	20	7.027586E-4	45.0	18
CTAACGC	20	7.027586E-4	45.0	23
CCGTACG	20	7.027586E-4	45.0	20
CCCCGAC	20	7.027586E-4	45.0	28
GTTCGCG	40	6.7957444E-9	45.0	1
GTTCGAC	20	7.027586E-4	45.0	25
GGACGAT	20	7.027586E-4	45.0	8
ACTAACG	20	7.027586E-4	45.0	16
CGTACGC	20	7.027586E-4	45.0	21
GCGGATA	25	3.8860802E-5	45.0	29
GGACCGT	20	7.027586E-4	45.0	8
CACAACG	20	7.027586E-4	45.0	12
ACGAGCA	25	3.8860802E-5	45.0	12
TCGCGCG	20	7.027586E-4	45.0	1
CTAGCTA	20	7.027586E-4	45.0	10
CGTTTTT	415	0.0	42.289154	1
>>END_MODULE