##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933890.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1100946
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.485232699878104	33.0	31.0	34.0	31.0	34.0
2	32.70819822225613	34.0	31.0	34.0	31.0	34.0
3	32.764982115380775	34.0	31.0	34.0	31.0	34.0
4	36.119234730858736	37.0	35.0	37.0	35.0	37.0
5	36.149149912893094	37.0	35.0	37.0	35.0	37.0
6	35.78177585458324	37.0	35.0	37.0	35.0	37.0
7	36.07857696926098	37.0	35.0	37.0	35.0	37.0
8	36.09518541327186	37.0	37.0	37.0	35.0	37.0
9	37.93712952315554	39.0	38.0	39.0	35.0	39.0
10	37.65927847505691	39.0	37.0	39.0	35.0	39.0
11	37.60367175138472	39.0	37.0	39.0	35.0	39.0
12	37.38885195095854	39.0	37.0	39.0	35.0	39.0
13	37.30700052500304	39.0	37.0	39.0	35.0	39.0
14	38.42287814297886	40.0	38.0	41.0	34.0	41.0
15	38.5289986974838	40.0	38.0	41.0	34.0	41.0
16	38.632494236774555	40.0	38.0	41.0	35.0	41.0
17	38.62726691409025	40.0	38.0	41.0	35.0	41.0
18	38.594587745448	40.0	38.0	41.0	35.0	41.0
19	38.600683412265454	40.0	38.0	41.0	35.0	41.0
20	38.55085444699377	40.0	38.0	41.0	34.0	41.0
21	38.47144455768039	40.0	38.0	41.0	34.0	41.0
22	38.488681552047055	40.0	38.0	41.0	34.0	41.0
23	38.45142631882036	40.0	37.0	41.0	34.0	41.0
24	38.43158429205429	40.0	37.0	41.0	34.0	41.0
25	38.338700535721095	40.0	37.0	41.0	34.0	41.0
26	38.25724422451238	40.0	37.0	41.0	34.0	41.0
27	38.22978783700563	40.0	37.0	41.0	34.0	41.0
28	38.1478446717641	40.0	37.0	41.0	34.0	41.0
29	38.09559233604555	40.0	37.0	41.0	34.0	41.0
30	38.02337898498201	40.0	37.0	41.0	34.0	41.0
31	37.94295633028323	40.0	36.0	41.0	34.0	41.0
32	37.84014747317307	40.0	36.0	41.0	34.0	41.0
33	37.788235753615524	40.0	36.0	41.0	33.0	41.0
34	37.71622132239002	40.0	36.0	41.0	33.0	41.0
35	37.63102186664923	40.0	36.0	41.0	33.0	41.0
36	37.546924190650586	40.0	36.0	41.0	33.0	41.0
37	37.49269446457864	40.0	35.0	41.0	33.0	41.0
38	37.44189088293159	40.0	35.0	41.0	33.0	41.0
39	37.38433401819889	39.0	35.0	41.0	33.0	41.0
40	37.26943192490822	39.0	35.0	41.0	33.0	41.0
41	37.16870400546439	39.0	35.0	41.0	32.0	41.0
42	37.16507712458195	39.0	35.0	41.0	32.0	41.0
43	37.047520041854916	39.0	35.0	41.0	32.0	41.0
44	36.93656273786362	39.0	35.0	41.0	32.0	41.0
45	36.94213340163823	39.0	35.0	41.0	32.0	41.0
46	36.863174033967155	39.0	35.0	41.0	32.0	41.0
47	36.79569933493559	39.0	35.0	41.0	32.0	41.0
48	36.73084420125964	39.0	35.0	41.0	31.0	41.0
49	36.703336040096424	39.0	35.0	41.0	31.0	41.0
50	36.61800487944004	39.0	35.0	41.0	31.0	41.0
51	35.69184047173976	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	27.0
10	30.0
11	25.0
12	16.0
13	19.0
14	22.0
15	28.0
16	61.0
17	85.0
18	151.0
19	277.0
20	463.0
21	843.0
22	1293.0
23	1792.0
24	2611.0
25	3804.0
26	5369.0
27	6440.0
28	7852.0
29	9556.0
30	12189.0
31	15781.0
32	21885.0
33	33640.0
34	66402.0
35	77600.0
36	78078.0
37	128834.0
38	242185.0
39	383310.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.612113582319207	4.452988611612195	53.24012258548557	15.694775220583027
2	20.692568027859675	4.347079693281959	52.05514166907369	22.905210609784675
3	22.119704327006048	4.9593713043146535	49.81661225891188	23.10431210976742
4	19.963286119391867	5.058740392353485	49.23820060202771	25.739772886226937
5	19.158977824525454	6.353354297122657	48.69439554710222	25.79327233124967
6	23.168166286084876	6.827491993249442	51.58926959178742	18.415072128878254
7	83.69929133672315	3.007141131354308	8.497691984892992	4.795875547029555
8	84.50441711037598	3.413518919184047	7.316889293389503	4.765174677050464
9	78.30438550119624	5.980038984655015	9.922830002561433	5.792745511587308
10	38.48617461710202	29.360295600329174	17.30257433152943	14.850955451039379
11	30.76281670490651	24.730004923038916	24.992869768362844	19.514308603691735
12	27.7139841554445	21.94512719061607	29.879939615566975	20.46094903837245
13	25.329580197393874	22.61228071131554	31.054747462636676	21.003391628653905
14	21.174789680874447	24.424631180820857	32.76236981650326	21.638209321801433
15	19.492236676458248	24.478948104630017	33.92682293227824	22.101992286633497
16	23.310770918827988	24.747626132435197	30.433372753977032	21.50823019475978
17	23.86774646531256	24.522910297144456	28.456254893518846	23.153088344024138
18	25.065443718402175	23.45419303035753	30.083582664363195	21.396780586877103
19	24.881601822432707	25.00876518921001	28.33426889238891	21.775364095968378
20	25.565649904718306	27.07898480034443	28.41792422153312	18.937441073404145
21	25.628777433225608	25.881105885302276	29.4820091085303	19.008107572941814
22	24.3372517816496	22.734357543421748	30.31093259796575	22.617458076962905
23	22.742441500309734	24.909577763123714	29.606810869924594	22.74116986664196
24	22.605559219071598	24.403921718231413	29.490819713228444	23.499699349468546
25	22.54370332423207	26.378768804282863	26.999780189037427	24.07774768244764
26	21.4104960642938	27.297705791201388	27.277813807398367	24.013984337106454
27	21.26007996759151	27.149742130858375	29.026491762538765	22.56368613901136
28	19.81214337487942	26.573782910333478	29.739060771372984	23.875012943414117
29	20.337782234551014	26.69240816534144	29.77820892214514	23.191600677962406
30	22.83735987051136	26.13988333669408	28.937477405794652	22.085279386999908
31	23.352008182054345	26.50030065053145	27.210780546911472	22.936910620502733
32	23.251821615229083	27.038292522975695	26.717295852839285	22.992590008955936
33	24.014075168082723	26.136613421548375	26.02779791197752	23.821513498391383
34	21.03881570939901	25.551026117538918	29.16827891649545	24.241879256566627
35	20.400001453295623	28.068588286800622	27.318960239648447	24.212450020255307
36	23.016296893762274	28.334995540199063	26.0099950406287	22.638712525409964
37	22.749980471340102	27.649403331316886	26.85626724653162	22.744348950811393
38	22.539888423228753	27.3345831675668	26.823840588003407	23.30168782120104
39	21.842851511336615	25.386258726586046	28.00982064515426	24.761069116923082
40	22.810383070559322	25.124847176882426	27.749226574237063	24.31554317832119
41	20.473847037002724	25.23084692618893	26.674151139111274	27.62115489769707
42	22.15549173165623	24.6803203790195	27.45093764816803	25.713250241156242
43	23.00276307829812	24.59094269837031	28.249614422505736	24.156679800825838
44	22.01606618308255	24.737089739187933	28.25588175986833	24.990962317861186
45	21.76737097005666	24.41518475928883	26.701037108087043	27.116407162567462
46	21.17042979401351	25.572189735009708	28.329909005527977	24.927471465448807
47	19.388961856439828	26.171310854483327	30.092847423942683	24.346879865134166
48	20.481295177056822	25.997914520784853	29.03021583256581	24.490574469592513
49	22.856616037480492	24.11934826957907	29.38854403394899	23.63549165899145
50	21.200858171063793	24.742721259716642	27.73242284362721	26.323997725592353
51	19.864371186234383	24.485033780040073	26.407562223760294	29.243032809965246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	587.5
2	1024.0
3	1925.0
4	2826.0
5	2014.0
6	1202.0
7	1288.5
8	1375.0
9	1500.5
10	1626.0
11	1657.5
12	1689.0
13	1703.0
14	1717.0
15	1614.5
16	1512.0
17	1613.5
18	1715.0
19	1878.0
20	2041.0
21	2540.5
22	3040.0
23	3845.5
24	4651.0
25	5642.0
26	8182.0
27	9731.0
28	11547.0
29	13363.0
30	15782.0
31	18201.0
32	21415.0
33	24629.0
34	27966.5
35	31304.0
36	33455.0
37	35606.0
38	39499.0
39	43392.0
40	47167.5
41	50943.0
42	56100.5
43	61258.0
44	68995.0
45	76732.0
46	83360.0
47	89988.0
48	101831.0
49	113674.0
50	111932.0
51	110190.0
52	96458.5
53	82727.0
54	74485.0
55	66243.0
56	61639.5
57	57036.0
58	54208.5
59	51381.0
60	45934.0
61	40487.0
62	35043.0
63	29599.0
64	25186.0
65	20773.0
66	17944.0
67	15115.0
68	12682.0
69	10249.0
70	8981.5
71	7714.0
72	5946.0
73	4178.0
74	3451.0
75	2029.0
76	1334.0
77	915.0
78	496.0
79	427.5
80	359.0
81	247.5
82	136.0
83	129.0
84	122.0
85	82.0
86	42.0
87	26.5
88	11.0
89	8.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1100946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.42277576818931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28503111489557	19.976956792388854
2	11.845656258843428	6.733728633443101
3	4.564243998706811	3.891854511796423
4	2.2896149735876277	2.6030885195907936
5	1.3567388718674473	1.9281142365537292
6	0.9150505257899662	1.560496554665516
7	0.6529155120813692	1.299036983682288
8	0.5323451639923228	1.2104581781947006
9	0.40939700770343534	1.047257941511017
>10	6.673503627196875	47.70277081938407
>50	0.411909051810438	6.980607294882933
>100	0.058428907248718034	2.8643148485751153
>500	0.0029053047802994197	0.5599626344022106
>1k	0.0022596814957884376	1.6413520509292387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	3848	0.3495175966850327	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	3741	0.3397986822241963	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	3689	0.33507547145818234	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2955	0.26840553487637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.12925247923149727	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1126	0.10227567927945604	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1107	0.10054989073033556	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.816619525389983E-4	0.0	0.0	0.3483367939935292	0.0
2	1.816619525389983E-4	0.0	0.0	1.367187854808501	0.0
3	1.816619525389983E-4	0.0	0.0	1.9359714282081046	0.0
4	1.816619525389983E-4	0.0	0.0	2.568972501830244	0.0
5	1.816619525389983E-4	0.0	0.0	3.575833873777642	0.0
6	1.816619525389983E-4	0.0	0.0	4.229635240965497	0.0
7	1.816619525389983E-4	0.0	0.0	4.824123980649369	0.0
8	1.816619525389983E-4	0.0	0.0	5.727528870625807	0.0
9	1.816619525389983E-4	0.0	0.0	6.189586046908749	0.0
10	1.816619525389983E-4	0.0	0.0	7.082817867543004	0.0
11	1.816619525389983E-4	0.0	0.0	8.708692342767039	0.0
12	1.816619525389983E-4	0.0	0.0	10.153994837167309	0.0
13	1.816619525389983E-4	0.0	0.0	10.640213053137938	0.0
14	1.816619525389983E-4	0.0	0.0	10.83386469454451	0.0
15	1.816619525389983E-4	0.0	0.0	11.134242733067744	0.0
16	1.816619525389983E-4	0.0	0.0	11.780141805320152	0.0
17	1.816619525389983E-4	0.0	0.0	12.63858536204319	0.0
18	1.816619525389983E-4	0.0	0.0	13.554979081626165	0.0
19	1.816619525389983E-4	0.0	0.0	14.189887605749965	0.0
20	1.816619525389983E-4	0.0	0.0	14.957318524250962	0.0
21	1.816619525389983E-4	0.0	0.0	15.973989641635466	0.0
22	1.816619525389983E-4	0.0	0.0	17.040526964991926	0.0
23	1.816619525389983E-4	0.0	0.0	17.972816105422066	0.0
24	1.816619525389983E-4	0.0	0.0	18.697283972147588	0.0
25	1.816619525389983E-4	0.0	0.0	19.352629465932026	0.0
26	1.816619525389983E-4	0.0	0.0	19.940760037277034	0.0
27	1.816619525389983E-4	0.0	0.0	20.58130008192954	0.0
28	1.816619525389983E-4	0.0	0.0	21.210304592595822	0.0
29	1.816619525389983E-4	0.0	0.0	21.90361743446091	0.0
30	1.816619525389983E-4	0.0	0.0	22.725456107747338	0.0
31	1.816619525389983E-4	0.0	0.0	23.389612206229916	0.0
32	1.816619525389983E-4	0.0	0.0	23.997453099425403	0.0
33	1.816619525389983E-4	0.0	0.0	24.616738695630847	0.0
34	2.7249292880849745E-4	0.0	0.0	25.302421735489297	0.0
35	2.7249292880849745E-4	0.0	0.0	26.0144457584659	0.0
36	2.7249292880849745E-4	0.0	0.0	26.63300470686119	0.0
37	2.7249292880849745E-4	0.0	0.0	27.27545220201536	0.0
38	3.633239050779966E-4	0.0	0.0	27.99001949232751	0.0
39	3.633239050779966E-4	0.0	0.0	29.042387183385923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTC	25	3.890699E-5	45.0	23
ACTCGTA	45	1.9281288E-8	40.0	11
CGGTAGT	405	0.0	40.0	12
ACGGTTA	90	0.0	40.0	34
GGACCGT	85	0.0	39.705883	8
TCTTGCG	240	0.0	39.375004	1
CGTTAGG	80	0.0	39.375	2
CGTTTTT	1000	0.0	38.925003	1
TTTCTCG	430	0.0	38.720932	1
TCGTTTG	350	0.0	38.57143	1
TACGCGG	35	6.2480485E-6	38.571426	2
TTGGGAC	2005	0.0	38.491272	5
TTTAGCG	130	0.0	38.076927	1
ACGCCGG	345	0.0	37.826084	27
TATCTCG	30	1.13989045E-4	37.500004	1
GCGGATA	30	1.13989045E-4	37.500004	5
GCGCTTA	30	1.13989045E-4	37.500004	43
TTTGGGA	5505	0.0	37.35695	4
CGTTTGG	670	0.0	37.27612	2
TTTGTCG	200	0.0	37.125	1
>>END_MODULE