##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933889.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344620
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44620741686495	33.0	31.0	34.0	31.0	34.0
2	32.66360048749347	34.0	31.0	34.0	31.0	34.0
3	32.72446752945273	34.0	31.0	34.0	31.0	34.0
4	36.092002785676975	37.0	35.0	37.0	35.0	37.0
5	36.142940049910045	37.0	35.0	37.0	35.0	37.0
6	35.79781498462074	37.0	35.0	37.0	35.0	37.0
7	36.1288346584644	37.0	35.0	37.0	35.0	37.0
8	36.12927862573269	37.0	37.0	37.0	35.0	37.0
9	37.98288259532239	39.0	39.0	39.0	35.0	39.0
10	37.62855318901979	39.0	37.0	39.0	35.0	39.0
11	37.53935929429517	39.0	37.0	39.0	35.0	39.0
12	37.430111427079105	39.0	37.0	39.0	35.0	39.0
13	37.38220358655911	39.0	37.0	39.0	35.0	39.0
14	38.635813359642505	40.0	38.0	41.0	35.0	41.0
15	38.695818582786835	40.0	38.0	41.0	35.0	41.0
16	38.76079449828797	40.0	38.0	41.0	35.0	41.0
17	38.72447333294643	40.0	38.0	41.0	35.0	41.0
18	38.675277116824326	40.0	38.0	41.0	35.0	41.0
19	38.71990017990831	40.0	38.0	41.0	35.0	41.0
20	38.664642214613195	40.0	38.0	41.0	35.0	41.0
21	38.59317509140502	40.0	38.0	41.0	35.0	41.0
22	38.58735128547386	40.0	38.0	41.0	35.0	41.0
23	38.52281353374732	40.0	38.0	41.0	34.0	41.0
24	38.4867970518252	40.0	38.0	41.0	34.0	41.0
25	38.42104636991469	40.0	38.0	41.0	34.0	41.0
26	38.37912773489641	40.0	38.0	41.0	34.0	41.0
27	38.375143636469154	40.0	38.0	41.0	34.0	41.0
28	38.31811560559457	40.0	37.0	41.0	34.0	41.0
29	38.33274331147351	40.0	37.0	41.0	34.0	41.0
30	38.26117462712553	40.0	37.0	41.0	34.0	41.0
31	38.21757297893332	40.0	37.0	41.0	34.0	41.0
32	38.10689745226627	40.0	37.0	41.0	34.0	41.0
33	38.0163687539899	40.0	37.0	41.0	34.0	41.0
34	38.02615344437351	40.0	37.0	41.0	34.0	41.0
35	37.997373919099296	40.0	37.0	41.0	34.0	41.0
36	37.90254773373571	40.0	37.0	41.0	34.0	41.0
37	37.85333700887934	40.0	37.0	41.0	33.0	41.0
38	37.806824908594976	40.0	37.0	41.0	33.0	41.0
39	37.754370030758515	40.0	37.0	41.0	33.0	41.0
40	37.6841709709245	40.0	36.0	41.0	33.0	41.0
41	37.609717950206026	40.0	36.0	41.0	33.0	41.0
42	37.57315884162266	40.0	36.0	41.0	33.0	41.0
43	37.46284022981835	40.0	36.0	41.0	33.0	41.0
44	37.37194881318554	40.0	36.0	41.0	33.0	41.0
45	37.39473332946434	40.0	36.0	41.0	33.0	41.0
46	37.312433985259126	39.0	35.0	41.0	33.0	41.0
47	37.23567697754048	39.0	35.0	41.0	32.0	41.0
48	37.1997301375428	39.0	35.0	41.0	32.0	41.0
49	37.18170738784748	39.0	35.0	41.0	32.0	41.0
50	37.099550229238	39.0	35.0	41.0	32.0	41.0
51	36.17195751842609	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	6.0
12	5.0
13	4.0
14	2.0
15	3.0
16	5.0
17	12.0
18	31.0
19	60.0
20	136.0
21	191.0
22	352.0
23	481.0
24	682.0
25	1097.0
26	1532.0
27	1882.0
28	2304.0
29	2840.0
30	3491.0
31	4692.0
32	6080.0
33	8794.0
34	16673.0
35	24926.0
36	22454.0
37	35864.0
38	73835.0
39	136080.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.456851024316638	4.345075735592826	55.738784748418546	16.459288491671987
2	18.24067088387209	5.087342580233301	55.6363530845569	21.035633451337706
3	19.482908711044047	5.069641924438512	54.384249318089495	21.063200046427948
4	18.098195113458303	4.454761766583483	53.13881956938077	24.30822355057745
5	17.834716499332597	5.295978178863676	52.780163658522426	24.089141663281296
6	20.977888688990774	6.387615344437352	53.87238117346642	18.76211479310545
7	84.12077070396379	1.9267599094654981	9.336660669723173	4.615808716847543
8	85.64273692763044	2.740699901340607	6.849863617897975	4.766699553130985
9	79.91904126284022	5.344727525970634	9.139051709128895	5.59717950206024
10	35.62416574778016	37.785676977540476	14.773953920259997	11.816203354419361
11	23.941442748534616	23.73803029423713	32.247402936567816	20.073124020660437
12	22.725900992397424	22.633045093146073	33.63095583541292	21.010098079043583
13	21.522256398351807	24.954152399744647	34.50844408333817	19.015147118565377
14	19.072311531541988	27.775230688874704	31.68417387267135	21.46828390691196
15	16.24455922465324	26.962161221055077	36.37136556206836	20.421913992223317
16	18.778364575474434	27.135105333410714	31.965062967906682	22.12146712320817
17	19.401369624513958	25.969183448435956	30.78405199930358	23.8453949277465
18	19.19795717021647	25.71411990017991	32.707910161917475	22.380012767686146
19	20.882711392258138	26.199291973768208	31.049852010910566	21.868144623063085
20	23.299576344959664	26.714352039928034	30.958156810399863	19.027914804712438
21	23.014044454761766	27.721548372120015	30.49068539260635	18.773721780511867
22	21.905867332133944	24.759155011316814	29.95560327317045	23.379374383378796
23	19.871742789159075	26.713481515872555	29.374093204108874	24.040682490859496
24	20.59137600835703	24.49219430096918	31.212930183970748	23.703499506703036
25	18.324531367883466	28.07643201207127	29.002959781788633	24.596076838256632
26	17.76739597237537	28.61180430619233	28.82131042887818	24.799489292554117
27	18.517787708200338	28.236028088909528	31.35482560501422	21.89135859787592
28	16.694039811966803	25.967152225639833	34.85781440427137	22.48099355812199
29	19.17996633973652	23.67506238755731	32.82891300562939	24.31605826707678
30	19.501189716209158	25.040334281237303	33.39011084672973	22.068365155823805
31	21.090766641518194	26.42504787882305	29.183158261273284	23.301027218385467
32	22.063432186176076	27.26974638732517	28.21078289130056	22.45603853519819
33	20.83802449074343	26.75004352620277	29.119900179908303	23.292031803145495
34	20.21095699611166	24.6895130868783	29.58185827868377	25.517671638326274
35	19.650339504381638	24.17909581568104	29.560385351981893	26.61017932795543
36	19.659625094306772	25.72137426730892	31.434623643433348	23.184376994950963
37	19.318089489872904	25.855434971853054	33.114735070512445	21.711740467761594
38	20.277116824328246	26.704776275317744	30.715860948290867	22.302245952063142
39	20.72804828506761	26.116592188497474	30.811908769078983	22.343450757355928
40	21.622076490047007	24.299228135337472	30.54697928152748	23.53171609308804
41	19.96198711624398	24.71911090476467	27.649875224885378	27.669026754105975
42	20.93900528117927	25.44077534675875	28.02855318901979	25.59166618304219
43	21.22424699669201	24.923103708432475	30.281179269920493	23.571470024955023
44	19.520051070744586	24.285589925134932	30.44077534675875	25.753583657361734
45	20.870233880796242	22.818466716963613	29.137600835703093	27.17369856653706
46	21.69781208287389	23.729905403052637	31.124136730311648	23.448145783761827
47	18.648076141837386	23.906041436945042	33.43305670013348	24.012825721084095
48	19.529046485984562	23.518077882885496	32.142940049910045	24.809935581219893
49	21.289826475538273	22.17660031338866	33.67651326098311	22.857059950089955
50	21.099471882073008	22.52539028495154	30.57425570193256	25.80088213104289
51	19.575184260925077	22.391329580407405	28.776913702048635	29.256572456618883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	139.5
2	232.0
3	621.5
4	1011.0
5	707.5
6	404.0
7	418.0
8	432.0
9	484.0
10	536.0
11	601.0
12	666.0
13	670.0
14	674.0
15	719.0
16	764.0
17	772.0
18	780.0
19	787.5
20	795.0
21	1155.0
22	1515.0
23	1633.5
24	1752.0
25	2246.5
26	3428.5
27	4116.0
28	4588.0
29	5060.0
30	6040.5
31	7021.0
32	8151.0
33	9281.0
34	10362.0
35	11443.0
36	11673.0
37	11903.0
38	14116.0
39	16329.0
40	17732.5
41	19136.0
42	21503.5
43	23871.0
44	25828.5
45	27786.0
46	32806.5
47	37827.0
48	40385.5
49	42944.0
50	40220.0
51	37496.0
52	30505.0
53	23514.0
54	19670.0
55	15826.0
56	13636.0
57	11446.0
58	10397.0
59	9348.0
60	7964.5
61	6581.0
62	5419.0
63	4257.0
64	3607.0
65	2957.0
66	2359.0
67	1761.0
68	1350.5
69	940.0
70	794.5
71	649.0
72	498.5
73	348.0
74	275.0
75	164.0
76	126.0
77	95.0
78	64.0
79	49.5
80	35.0
81	18.5
82	2.0
83	2.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	344620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0341933058642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96564792403271	25.932240686763592
2	11.429663006950415	8.237173724266727
3	4.123785707207727	4.457918739764494
4	2.0382887787620887	2.937923674683479
5	1.243257210714719	2.2399885329901865
6	0.8467161651895011	1.8306440382983118
7	0.6794884755924641	1.7139373355024086
8	0.6310344264513515	1.819105320432252
9	0.5777676723479868	1.8737452792139908
>10	6.344117438983226	41.803162854899206
>50	0.07343101095887396	1.8170356313036613
>100	0.038732840945340116	2.2566832348452097
>500	0.0048416051181675145	1.044453830635742
>1k	0.003227736745445009	2.035987116400742
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	2018	0.5855725146538215	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	1891	0.5487203296384423	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1570	0.45557425570193255	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1523	0.4419360454993906	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	718	0.20834542394521502	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	647	0.1877430212988219	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	596	0.1729441123556381	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.17004236550403343	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	526	0.15263188439440542	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	519	0.15060066159828217	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	375	0.10881550693517497	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	363	0.10533341071324938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.901746851604666E-4	0.0	0.0	0.5780279728396495	0.0
2	2.901746851604666E-4	0.0	0.0	2.170796819685451	0.0
3	2.901746851604666E-4	0.0	0.0	3.0810748070338345	0.0
4	2.901746851604666E-4	0.0	0.0	4.0090534501770065	0.0
5	2.901746851604666E-4	0.0	0.0	5.550751552434566	0.0
6	2.901746851604666E-4	0.0	0.0	6.283732807149904	0.0
7	2.901746851604666E-4	0.0	0.0	7.0422494341593636	0.0
8	2.901746851604666E-4	0.0	0.0	8.21513551157797	0.0
9	2.901746851604666E-4	0.0	0.0	8.70727177761012	0.0
10	2.901746851604666E-4	0.0	0.0	9.86971156636295	0.0
11	2.901746851604666E-4	0.0	0.0	12.005977598514306	0.0
12	2.901746851604666E-4	0.0	0.0	14.25976437815565	0.0
13	2.901746851604666E-4	0.0	0.0	14.90105043236028	0.0
14	2.901746851604666E-4	0.0	0.0	15.091114851140386	0.0
15	2.901746851604666E-4	0.0	0.0	15.413789101038825	0.0
16	2.901746851604666E-4	0.0	0.0	16.11398061633103	0.0
17	2.901746851604666E-4	0.0	0.0	17.177761012129302	0.0
18	2.901746851604666E-4	0.0	0.0	18.344843595844697	0.0
19	2.901746851604666E-4	0.0	0.0	19.3166386164471	0.0
20	2.901746851604666E-4	0.0	0.0	20.277116824328246	0.0
21	2.901746851604666E-4	0.0	0.0	21.55997910742267	0.0
22	2.901746851604666E-4	0.0	0.0	22.909581568104	0.0
23	2.901746851604666E-4	0.0	0.0	24.12222157738959	0.0
24	2.901746851604666E-4	0.0	0.0	25.020892577331555	0.0
25	2.901746851604666E-4	0.0	0.0	25.82148453368928	0.0
26	2.901746851604666E-4	0.0	0.0	26.468574081597122	0.0
27	2.901746851604666E-4	0.0	0.0	27.132783935929428	0.0
28	2.901746851604666E-4	0.0	0.0	27.84226104114677	0.0
29	2.901746851604666E-4	0.0	0.0	28.561313910974405	0.0
30	2.901746851604666E-4	0.0	0.0	29.475654343915036	0.0
31	5.803493703209332E-4	0.0	0.0	30.2869827636237	0.0
32	5.803493703209332E-4	0.0	0.0	31.044048517207358	0.0
33	5.803493703209332E-4	0.0	0.0	31.76658348325692	0.0
34	5.803493703209332E-4	0.0	0.0	32.45719923393883	0.0
35	5.803493703209332E-4	0.0	0.0	33.20265800011607	0.0
36	5.803493703209332E-4	0.0	0.0	33.93186698392432	0.0
37	5.803493703209332E-4	0.0	0.0	34.61290696999594	0.0
38	5.803493703209332E-4	0.0	0.0	35.35430329058093	0.0
39	5.803493703209332E-4	0.0	0.0	36.06494109453891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTCG	35	1.2086093E-7	45.000004	1
GCGATCT	95	0.0	45.000004	9
GATCGCT	20	7.0264057E-4	45.0	9
GGATGCG	20	7.0264057E-4	45.0	8
GGATCGC	20	7.0264057E-4	45.0	8
TGCGCAT	20	7.0264057E-4	45.0	13
CGCTCAC	20	7.0264057E-4	45.0	38
CTACGGG	20	7.0264057E-4	45.0	3
GTCGTTG	20	7.0264057E-4	45.0	1
TCGGTCG	20	7.0264057E-4	45.0	2
TAGTCCA	20	7.0264057E-4	45.0	18
CTACGAA	50	2.1827873E-11	44.999996	11
GCGGGTC	30	2.161063E-6	44.999996	5
CGGGACG	30	2.161063E-6	44.999996	6
CGCCGGG	25	3.8851034E-5	44.999996	3
ACATACG	30	2.161063E-6	44.999996	17
AGGGCCG	25	3.8851034E-5	44.999996	6
TGCGGGC	140	0.0	43.39286	4
GGACCGA	180	0.0	42.5	8
CGTTTGG	335	0.0	42.31343	2
>>END_MODULE