##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933888.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381027
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38799350177284	33.0	31.0	34.0	31.0	34.0
2	32.62579554729717	34.0	31.0	34.0	31.0	34.0
3	32.67071099948298	34.0	31.0	34.0	31.0	34.0
4	36.046085972910056	37.0	35.0	37.0	35.0	37.0
5	36.087006957512195	37.0	35.0	37.0	35.0	37.0
6	35.74028349697003	37.0	35.0	37.0	35.0	37.0
7	36.05822422033084	37.0	35.0	37.0	35.0	37.0
8	36.059709679366556	37.0	36.0	37.0	35.0	37.0
9	37.91496140693442	39.0	38.0	39.0	35.0	39.0
10	37.51418928317416	39.0	37.0	39.0	35.0	39.0
11	37.466407892354084	39.0	37.0	39.0	35.0	39.0
12	37.17973791883515	39.0	37.0	39.0	34.0	39.0
13	37.05749986221449	39.0	37.0	39.0	33.0	39.0
14	38.21447823907492	40.0	37.0	41.0	33.0	41.0
15	38.29374821206896	40.0	37.0	41.0	34.0	41.0
16	38.373159907303155	40.0	37.0	41.0	34.0	41.0
17	38.35882758964588	40.0	37.0	41.0	34.0	41.0
18	38.303967960275784	40.0	37.0	41.0	34.0	41.0
19	38.352707288459875	40.0	37.0	41.0	34.0	41.0
20	38.30422253541088	40.0	37.0	41.0	34.0	41.0
21	38.19269763035165	40.0	37.0	41.0	34.0	41.0
22	38.20296461930519	40.0	37.0	41.0	34.0	41.0
23	38.156214651455144	40.0	36.0	41.0	34.0	41.0
24	38.12397546630554	40.0	36.0	41.0	34.0	41.0
25	38.028312954200096	40.0	36.0	41.0	34.0	41.0
26	37.96007632005081	40.0	36.0	41.0	34.0	41.0
27	37.940219459513365	40.0	36.0	41.0	34.0	41.0
28	37.90329031800896	40.0	36.0	41.0	34.0	41.0
29	37.884480627357114	40.0	36.0	41.0	34.0	41.0
30	37.80391153382831	40.0	36.0	41.0	34.0	41.0
31	37.72769383796949	40.0	36.0	41.0	33.0	41.0
32	37.6169641521467	40.0	35.0	41.0	33.0	41.0
33	37.52252465048409	40.0	35.0	41.0	33.0	41.0
34	37.48912281806801	40.0	35.0	41.0	33.0	41.0
35	37.4318302902419	40.0	35.0	41.0	33.0	41.0
36	37.36004535111685	40.0	35.0	41.0	33.0	41.0
37	37.28665422660337	39.0	35.0	41.0	33.0	41.0
38	37.214549100195	39.0	35.0	41.0	33.0	41.0
39	37.127744228099324	39.0	35.0	41.0	32.0	41.0
40	37.08780480123456	39.0	35.0	41.0	32.0	41.0
41	37.038640306329995	39.0	35.0	41.0	32.0	41.0
42	37.013298270201325	39.0	35.0	41.0	32.0	41.0
43	36.87381996551426	39.0	35.0	41.0	32.0	41.0
44	36.71470263262184	39.0	35.0	41.0	31.0	41.0
45	36.68272852055104	39.0	35.0	41.0	31.0	41.0
46	36.57748401031948	39.0	35.0	41.0	31.0	41.0
47	36.492568768092546	39.0	35.0	40.0	31.0	41.0
48	36.36948300251688	38.0	35.0	40.0	31.0	41.0
49	36.31901938707755	38.0	35.0	40.0	31.0	41.0
50	36.1712004661087	38.0	35.0	40.0	31.0	41.0
51	35.19371068192018	37.0	34.0	39.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	15.0
11	7.0
12	11.0
13	10.0
14	7.0
15	10.0
16	10.0
17	21.0
18	41.0
19	100.0
20	191.0
21	285.0
22	470.0
23	654.0
24	947.0
25	1493.0
26	2100.0
27	2612.0
28	3075.0
29	3735.0
30	4658.0
31	6019.0
32	8269.0
33	12020.0
34	24258.0
35	35715.0
36	28076.0
37	44386.0
38	83923.0
39	117842.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.419550320580957	4.807533324410081	57.15894149233519	17.61397486267377
2	19.999107674784202	4.6623992525464075	53.80458602671202	21.533907045957374
3	20.095163859778967	4.716463662680073	52.255089534337465	22.9332829432035
4	19.958953040073276	4.836402669627087	50.88274584215817	24.32189844814147
5	18.18899972967795	5.457618488978471	51.03102929713642	25.322352484207155
6	20.72241599676663	6.113739971183144	54.29641468977265	18.867429342277582
7	83.34370005275217	2.1869841244846167	9.77621008484963	4.693105737913586
8	84.16358945691512	2.8352321489028336	7.625706314775593	5.375472079406447
9	78.56109934466586	5.098326365323192	10.544134667621979	5.796439622388965
10	39.90084692160923	24.39354691399822	20.434772338968106	15.270833825424447
11	31.598023237198415	22.892340962713927	24.01299645431951	21.49663934576815
12	26.269004558732057	20.072068383605362	30.928254428163882	22.730672629498695
13	23.47235235298286	25.29164599883998	31.564692265902416	19.671309382274746
14	20.2597191275159	26.88339671466851	31.763628299306873	21.093255858508712
15	17.872749175255297	25.048356153238483	36.85224406669344	20.226650604812782
16	19.845050350762545	24.04396538827957	33.10474060893323	23.00624365202466
17	20.319819855285846	23.275253459728575	29.07116818493178	27.3337585000538
18	20.731339248924616	23.47523928750456	33.027580722625956	22.765840740944867
19	23.02618974508368	26.007343311628834	29.46930270033357	21.49716424295391
20	25.860109651022107	26.17006143921559	28.34208599390594	19.627742915856356
21	24.17413989034898	26.940610507916762	29.13258115566613	19.752668446068125
22	22.243305592517064	24.534744256968665	28.48800741154826	24.733942738966004
23	20.773855920971478	26.396029677686883	27.306463846394085	25.52365055494755
24	22.389751907345147	23.537177155424683	30.410705803000837	23.662365134229333
25	20.442908245347443	25.092972414028402	28.616344773467496	25.847774567156655
26	20.300136210819705	26.621735467565287	28.352321489028338	24.725806832586667
27	19.686531400661895	27.085219682594676	29.37272161815304	23.85552729859039
28	18.66481902857278	25.139950712154256	31.769139719757394	24.42609053951557
29	20.034800683416133	25.45068984612639	29.999448857954945	24.515060612502527
30	21.405044786852375	26.148015757413518	29.36248612303065	23.084453332703454
31	23.717741787327405	27.36210294808504	26.408364761552335	22.51179050303522
32	24.25759854288541	27.56786264490443	24.69405055284796	23.480488259362197
33	22.61886952893102	28.369118198972775	25.059903891325284	23.952108380770916
34	20.42269970369554	25.975062134704363	28.89506517910804	24.707172982492054
35	19.828516089410986	26.67238804599149	27.330084219753452	26.169011644844066
36	23.756584179073926	26.560322496830935	26.842979631364706	22.840113692730437
37	21.541518055150945	27.56786264490443	28.24786694906135	22.64275235088327
38	20.725565379881218	30.294965973539934	25.10399525492944	23.87547339164941
39	20.284126846653912	30.41936660656594	27.125374317305596	22.17113222947455
40	21.758825490057134	26.049597535082814	28.9000517023728	23.291525272487252
41	18.762974802310598	25.15832211365599	27.697774698380957	28.38092838565246
42	22.498930521984008	24.382786521690065	25.381140969012694	27.737141987313237
43	22.456676298530027	24.801654475929528	26.598902439984567	26.142766785555878
44	21.577998409561527	24.94495140764303	27.396483713752566	26.080566469042875
45	21.229991575400167	25.355421006910273	25.793710157022993	27.620877260666564
46	22.705740013174918	26.00419392851425	27.67730370813617	23.61276235017466
47	19.26477651190073	25.626792851950125	30.10075401480735	25.007676621341794
48	19.873394798793786	24.68670199224727	28.9926960556601	26.447207153298848
49	21.821813152348785	23.223288638337966	30.78889422534361	24.166003983969638
50	20.52531710351235	24.326884971406226	29.042823736900537	26.10497418818089
51	19.468436619977062	23.520642894073124	26.85164043492981	30.159280051020005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	185.5
2	295.0
3	799.0
4	1303.0
5	898.5
6	494.0
7	522.0
8	550.0
9	595.5
10	641.0
11	758.5
12	876.0
13	821.5
14	767.0
15	759.0
16	751.0
17	731.0
18	711.0
19	758.5
20	806.0
21	808.0
22	810.0
23	991.5
24	1173.0
25	1456.0
26	2230.5
27	2722.0
28	3199.0
29	3676.0
30	4409.0
31	5142.0
32	5577.5
33	6013.0
34	7134.5
35	8256.0
36	9018.5
37	9781.0
38	11496.5
39	13212.0
40	15567.0
41	17922.0
42	20911.0
43	23900.0
44	27261.5
45	30623.0
46	34767.0
47	38911.0
48	43752.0
49	48593.0
50	46493.0
51	44393.0
52	38042.5
53	31692.0
54	27198.5
55	22705.0
56	19270.5
57	15836.0
58	15032.5
59	14229.0
60	12925.0
61	11621.0
62	10090.0
63	8559.0
64	6926.5
65	5294.0
66	3893.5
67	2493.0
68	2109.0
69	1725.0
70	1471.5
71	1218.0
72	922.0
73	626.0
74	497.0
75	357.5
76	347.0
77	235.0
78	123.0
79	80.5
80	38.0
81	23.5
82	9.0
83	7.0
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	381027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.86140645209623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.29767045128797	23.10080321335289
2	12.633244240372221	8.302923475829505
3	5.060750964263026	4.989101831684557
4	2.4385168584662655	3.205323745053951
5	1.3872649865014772	2.279373928909342
6	0.8902588639218324	1.7553095024950056
7	0.6482987049898576	1.4912805072927537
8	0.5089962161398903	1.338106523292157
9	0.40094643416169246	1.1858097364655416
>10	5.42114033732312	41.90540114443936
>50	0.26009304689539886	5.3236121002129
>100	0.04721689159024163	2.7082229614183886
>500	0.0024008588944190662	0.5138733410964781
>1k	0.0032011451925587553	1.9008579884571872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	2644	0.6939140795796623	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1753	0.4600723833219168	No Hit
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGCT	1503	0.39446023510144956	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1328	0.3485317313471224	No Hit
TCCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	679	0.17820259456678922	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.17085403396609689	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	624	0.16376792195828643	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	452	0.11862676398260491	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	436	0.11442758649649501	No Hit
TGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	420	0.11022840901038508	No Hit
TTTTCGGGGGCCCTAAACAGGCAGAACTACTGAAGATGCCTTAAAATATAT	385	0.10104270825951966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40863245911707047	0.0
2	2.624485928818693E-4	0.0	0.0	2.024265996897858	0.0
3	2.624485928818693E-4	0.0	0.0	2.9798413235807435	0.0
4	2.624485928818693E-4	0.0	0.0	3.958774575030116	0.0
5	2.624485928818693E-4	0.0	0.0	5.454206657271007	0.0
6	2.624485928818693E-4	0.0	0.0	6.442588058064127	0.0
7	2.624485928818693E-4	0.0	0.0	7.3231030871827985	0.0
8	2.624485928818693E-4	0.0	0.0	8.79465234747144	0.0
9	2.624485928818693E-4	0.0	0.0	9.45313586701205	0.0
10	2.624485928818693E-4	0.0	0.0	10.686119356371071	0.0
11	2.624485928818693E-4	0.0	0.0	12.954462544649067	0.0
12	2.624485928818693E-4	0.0	0.0	15.096830408343767	0.0
13	2.624485928818693E-4	0.0	0.0	15.777359609686453	0.0
14	2.624485928818693E-4	0.0	0.0	16.00044091363604	0.0
15	2.624485928818693E-4	0.0	0.0	16.35527141121233	0.0
16	2.624485928818693E-4	0.0	0.0	17.268854963034116	0.0
17	2.624485928818693E-4	0.0	0.0	18.569287740763777	0.0
18	2.624485928818693E-4	0.0	0.0	20.146078886798048	0.0
19	2.624485928818693E-4	0.0	0.0	21.087481989465314	0.0
20	2.624485928818693E-4	0.0	0.0	22.094759688945928	0.0
21	2.624485928818693E-4	0.0	0.0	23.378658205324033	0.0
22	2.624485928818693E-4	0.0	0.0	24.617415563726453	0.0
23	2.624485928818693E-4	0.0	0.0	25.867983108808563	0.0
24	2.624485928818693E-4	0.0	0.0	26.81962170659822	0.0
25	2.624485928818693E-4	0.0	0.0	27.640298456539824	0.0
26	2.624485928818693E-4	0.0	0.0	28.38119083424534	0.0
27	2.624485928818693E-4	0.0	0.0	29.072217979303304	0.0
28	2.624485928818693E-4	0.0	0.0	29.76665695606873	0.0
29	2.624485928818693E-4	0.0	0.0	30.55846436079333	0.0
30	2.624485928818693E-4	0.0	0.0	31.42874389478961	0.0
31	2.624485928818693E-4	0.0	0.0	32.217139467806746	0.0
32	2.624485928818693E-4	0.0	0.0	32.93677350948883	0.0
33	2.624485928818693E-4	0.0	0.0	33.68921362528115	0.0
34	2.624485928818693E-4	0.0	0.0	34.40622318103441	0.0
35	2.624485928818693E-4	0.0	0.0	35.16338737149861	0.0
36	2.624485928818693E-4	0.0	0.0	35.87199857227966	0.0
37	2.624485928818693E-4	0.0	0.0	36.56801224060237	0.0
38	2.624485928818693E-4	0.0	0.0	37.25825203988169	0.0
39	2.624485928818693E-4	0.0	0.0	38.05347127631375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	20	7.027342E-4	45.000004	2
CACGGAT	20	7.027342E-4	45.000004	21
TGGGTCG	40	6.7939254E-9	45.000004	6
TGGCGAT	20	7.027342E-4	45.000004	21
TTCGTCG	35	1.209064E-7	45.000004	1
ACGGGGC	20	7.027342E-4	45.000004	5
ACGGGAG	30	2.161667E-6	45.000004	5
TATTGCG	30	2.161667E-6	45.000004	1
TGTTGCG	20	7.027342E-4	45.000004	1
AGTTGTC	20	7.027342E-4	45.000004	24
TTGACGG	20	7.027342E-4	45.000004	2
CGGGCTT	30	2.161667E-6	45.000004	6
TGTTCGC	20	7.027342E-4	45.000004	11
CGGGCTA	65	0.0	45.000004	6
GCTAGCG	20	7.027342E-4	45.000004	23
TACTAGC	20	7.027342E-4	45.000004	41
ATACGGT	20	7.027342E-4	45.000004	21
ACGAGGC	20	7.027342E-4	45.000004	24
ATGTTCG	20	7.027342E-4	45.000004	10
CCGCAAC	20	7.027342E-4	45.000004	25
>>END_MODULE