##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933885.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1642776
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.496487652607534	34.0	31.0	34.0	31.0	34.0
2	32.72509824833087	34.0	31.0	34.0	31.0	34.0
3	32.773981358383615	34.0	31.0	34.0	31.0	34.0
4	36.13233088382104	37.0	35.0	37.0	35.0	37.0
5	36.15193489556702	37.0	35.0	37.0	35.0	37.0
6	35.77994382679075	37.0	35.0	37.0	35.0	37.0
7	36.10252584649398	37.0	35.0	37.0	35.0	37.0
8	36.11907162023307	37.0	37.0	37.0	35.0	37.0
9	37.96244466683224	39.0	39.0	39.0	35.0	39.0
10	37.62977240962858	39.0	37.0	39.0	35.0	39.0
11	37.57162875522896	39.0	37.0	39.0	35.0	39.0
12	37.42041702581484	39.0	37.0	39.0	35.0	39.0
13	37.38389835254472	39.0	37.0	39.0	35.0	39.0
14	38.53740071683541	40.0	38.0	41.0	34.0	41.0
15	38.635257637072854	40.0	38.0	41.0	34.0	41.0
16	38.73746085893634	40.0	38.0	41.0	35.0	41.0
17	38.727401057721806	40.0	38.0	41.0	35.0	41.0
18	38.67839315889689	40.0	38.0	41.0	35.0	41.0
19	38.69352242789035	40.0	38.0	41.0	35.0	41.0
20	38.62751342848934	40.0	38.0	41.0	35.0	41.0
21	38.554707397721906	40.0	38.0	41.0	34.0	41.0
22	38.57135300247873	40.0	38.0	41.0	35.0	41.0
23	38.53327720882214	40.0	38.0	41.0	34.0	41.0
24	38.500010957062926	40.0	38.0	41.0	34.0	41.0
25	38.40346340584474	40.0	37.0	41.0	34.0	41.0
26	38.34571785806464	40.0	37.0	41.0	34.0	41.0
27	38.32681022853998	40.0	37.0	41.0	34.0	41.0
28	38.25444978499807	40.0	37.0	41.0	34.0	41.0
29	38.22211123123299	40.0	37.0	41.0	34.0	41.0
30	38.15070344343964	40.0	37.0	41.0	34.0	41.0
31	38.080419363321596	40.0	37.0	41.0	34.0	41.0
32	38.00859764204006	40.0	37.0	41.0	34.0	41.0
33	37.93936178760829	40.0	37.0	41.0	34.0	41.0
34	37.892912971701556	40.0	37.0	41.0	34.0	41.0
35	37.835547877495166	40.0	37.0	41.0	33.0	41.0
36	37.759567950834445	40.0	37.0	41.0	33.0	41.0
37	37.689489620009056	40.0	37.0	41.0	33.0	41.0
38	37.64748450184322	40.0	36.0	41.0	33.0	41.0
39	37.61540222160538	40.0	36.0	41.0	33.0	41.0
40	37.53395106819189	40.0	36.0	41.0	33.0	41.0
41	37.41128431386872	40.0	36.0	41.0	33.0	41.0
42	37.394729409243865	40.0	36.0	41.0	33.0	41.0
43	37.322405489245035	40.0	35.0	41.0	33.0	41.0
44	37.242883996357385	40.0	35.0	41.0	32.0	41.0
45	37.26189145689978	40.0	35.0	41.0	33.0	41.0
46	37.21362498599931	39.0	35.0	41.0	33.0	41.0
47	37.1350622361174	39.0	35.0	41.0	32.0	41.0
48	37.07383964703648	39.0	35.0	41.0	32.0	41.0
49	37.05314175517539	39.0	35.0	41.0	32.0	41.0
50	36.98902893638573	39.0	35.0	41.0	32.0	41.0
51	36.11712795901572	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	28.0
10	48.0
11	41.0
12	20.0
13	19.0
14	33.0
15	43.0
16	51.0
17	135.0
18	222.0
19	394.0
20	664.0
21	1136.0
22	1804.0
23	2780.0
24	3984.0
25	6159.0
26	8575.0
27	10438.0
28	12049.0
29	14218.0
30	17610.0
31	22481.0
32	30725.0
33	45537.0
34	87383.0
35	103715.0
36	105008.0
37	171172.0
38	346075.0
39	649766.0
40	461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.60534850764803	4.691083872664319	55.327567483333084	16.37600013635456
2	20.53524034926247	4.6828660754722495	53.549601406399894	21.23229216886538
3	21.37059465197933	4.6908403823771465	51.499839296410464	22.438725669233055
4	19.655083833705874	4.964888700589733	50.688590532123676	24.691436933580718
5	19.196287260101194	6.031863138979386	49.70726380224693	25.06458579867249
6	22.159137946987293	6.870626305716665	52.55232606271336	18.417909684582682
7	84.73261114114158	2.478244142841142	8.702586353830345	4.086558362186932
8	86.4157986237929	3.2138891729608905	6.528765942526553	3.8415462607196598
9	80.34698583373509	5.62164287766561	8.954964036484585	5.076407252114714
10	37.045525378992636	32.01185067227668	17.13775949977355	13.80486444895713
11	28.17200884356723	25.00468718802807	27.97228593551403	18.851018032890668
12	26.692196623276697	22.03264474280121	30.715812746229552	20.55934588769254
13	25.046811007708904	22.177886699099574	32.42164482558791	20.353657467603618
14	21.123938991073647	24.416232036504063	32.58989661402406	21.869932358398223
15	18.953223080931302	26.221408153028776	34.18865383959834	20.636714926441584
16	22.968195298689537	25.453196296999714	31.178261674141815	20.400346730168934
17	22.857650708313244	25.045471811129456	29.424583753354078	22.672293727203222
18	22.41334180679533	25.399080580675637	30.578605969407878	21.608971643121155
19	23.69988361164273	26.074157401861243	29.30210813890634	20.92385084758969
20	24.423475872547446	27.309627118974223	29.648960052983487	18.617936955494844
21	24.83820070417391	25.858059771995695	30.574101399095188	18.729638124735203
22	22.96393421866402	23.6082703910941	31.08397006043429	22.343825329807594
23	21.838339493637598	25.447352530107576	30.44316449716821	22.271143479086618
24	21.646651765061094	25.384775526304253	30.308331750646467	22.660240957988186
25	21.853557636585876	26.644229036703727	28.191792429399992	23.310420897310408
26	20.326630045727477	27.502288808699422	28.575046141409423	23.596035004163685
27	21.142931233473096	27.259102884385943	29.61334959848452	21.984616283656443
28	19.756436665741404	26.696518575874006	30.297861668298054	23.249183090086536
29	21.18560290630007	26.478351278567498	28.937542306437397	23.398503508695036
30	22.798726058817515	25.70131289962843	28.935107403565674	22.56485363798838
31	21.96671974754927	27.193360506849384	28.060003311467906	22.779916434133444
32	22.732314082991227	28.148146795424328	26.677769823761732	22.44176929782271
33	22.947863859710633	27.252772136919457	27.12968779675379	22.669676206616117
34	21.152853462675374	25.843145991906386	29.211103644075635	23.792896901342605
35	20.671716655222625	27.415667139037826	27.237614866542977	24.675001339196577
36	22.059550419533767	27.68088893434041	26.784905550117603	23.47465509600822
37	21.606049759675088	29.165692705517976	27.589762694366122	21.638494840440814
38	22.26195172074586	27.948728250230097	27.64466975412351	22.144650274900535
39	21.777588666988073	26.064661280661518	28.45397059611292	23.70377945623749
40	22.719713460630057	25.067751172405732	28.87399134148539	23.338544025478825
41	19.46613537086006	26.67107384086449	28.609317399328944	25.253473388946514
42	20.251878527565538	26.785149040404775	29.332361807087516	23.63061062494217
43	20.663864093461314	26.010728182052816	29.604218712715554	23.72118901177032
44	21.509931968813763	25.26899589475376	28.777873550624065	24.44319858580841
45	21.185542033728275	24.702515741647066	27.333123931686366	26.778818292938293
46	21.201247157250897	25.745932494752783	27.76093636624835	25.29188398174797
47	19.3173019328259	26.239974287425678	30.3186192152795	24.124104564468922
48	20.021841078759365	26.788557904425193	29.441810691171526	23.747790325643912
49	21.737291024461033	24.960006720331926	29.71878089283019	23.583921362376856
50	20.928477163045965	25.23539423512396	28.21108903465841	25.625039567171665
51	19.5682795463289	25.073655811869667	27.446773023224104	27.911291618577334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	894.0
2	1557.0
3	4260.0
4	6963.0
5	4730.0
6	2497.0
7	2406.0
8	2315.0
9	2453.0
10	2591.0
11	2576.0
12	2561.0
13	2567.5
14	2574.0
15	2602.5
16	2631.0
17	2757.5
18	2884.0
19	3617.5
20	4351.0
21	5231.0
22	6111.0
23	7084.5
24	8058.0
25	10285.0
26	15206.0
27	17900.0
28	21373.0
29	24846.0
30	29369.0
31	33892.0
32	36818.5
33	39745.0
34	45840.5
35	51936.0
36	56283.5
37	60631.0
38	65284.5
39	69938.0
40	77806.0
41	85674.0
42	90581.0
43	95488.0
44	103794.0
45	112100.0
46	126088.5
47	140077.0
48	154064.5
49	168052.0
50	166142.5
51	164233.0
52	144128.0
53	124023.0
54	110120.5
55	96218.0
56	87281.5
57	78345.0
58	72820.5
59	67296.0
60	59783.0
61	52270.0
62	44855.5
63	37441.0
64	31062.0
65	24683.0
66	19993.5
67	15304.0
68	12460.5
69	9617.0
70	8001.0
71	6385.0
72	5310.0
73	4235.0
74	3213.0
75	1767.0
76	1343.0
77	939.0
78	535.0
79	386.0
80	237.0
81	205.0
82	173.0
83	122.0
84	71.0
85	52.5
86	34.0
87	21.0
88	8.0
89	10.5
90	13.0
91	8.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1642776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.19245064248774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56017187296074	19.892641628538975
2	11.492670397676283	6.480130858737371
3	4.323190960734969	3.6564404333570923
4	2.139263860517039	2.412443631955376
5	1.3249165452780405	1.8676322154083262
6	0.8818782368661907	1.4917385199320514
7	0.6521974191093349	1.287093048317847
8	0.5086509963953406	1.147209448810228
9	0.44867588609765463	1.1384345495954322
>10	7.294619074002617	50.45281788151971
>50	0.3115200845143533	5.300182991726234
>100	0.05786124064654972	2.8977547168760815
>500	0.0024108838605106067	0.5208257969433046
>1k	0.001972541340417769	1.4546542782820355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3979	0.24221196316478935	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3817	0.23235060653430534	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	3763	0.22906348765747733	No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	3280	0.19966203548140463	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	2964	0.18042630279478153	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.826177153793335E-4	0.0	0.0	0.24215109059299625	0.0
2	1.826177153793335E-4	0.0	0.0	0.9121146157479778	0.0
3	1.826177153793335E-4	0.0	0.0	1.3681719236219667	0.0
4	1.826177153793335E-4	0.0	0.0	1.8687270814767198	0.0
5	1.826177153793335E-4	0.0	0.0	2.738778750115658	0.0
6	1.826177153793335E-4	0.0	0.0	3.418421014185744	0.0
7	1.826177153793335E-4	0.0	0.0	4.016554904624854	0.0
8	1.826177153793335E-4	0.0	0.0	4.922582263193521	0.0
9	1.826177153793335E-4	0.0	0.0	5.369873920729302	0.0
10	1.826177153793335E-4	0.0	0.0	6.209732793758857	0.0
11	1.826177153793335E-4	0.0	0.0	7.73221668687636	0.0
12	1.826177153793335E-4	0.0	0.0	9.207767827141375	0.0
13	1.826177153793335E-4	0.0	0.0	9.68233039684047	0.0
14	1.826177153793335E-4	0.0	0.0	9.860139179048147	0.0
15	1.826177153793335E-4	0.0	0.0	10.113064714848525	0.0
16	1.826177153793335E-4	0.0	0.0	10.67589251364763	0.0
17	1.826177153793335E-4	0.0	0.0	11.419816213531242	0.0
18	1.826177153793335E-4	0.0	0.0	12.214325020574929	0.0
19	1.826177153793335E-4	0.0	0.0	12.820128855059972	0.0
20	2.434902871724447E-4	0.0	0.0	13.512067378632267	0.0
21	2.434902871724447E-4	0.0	0.0	14.436904361885004	0.0
22	2.434902871724447E-4	0.0	0.0	15.471920700083274	0.0
23	3.043628589655559E-4	0.0	0.0	16.38281786439539	0.0
24	3.043628589655559E-4	0.0	0.0	17.070495307942167	0.0
25	3.043628589655559E-4	0.0	0.0	17.703022201444384	0.0
26	3.043628589655559E-4	0.0	0.0	18.262806371653834	0.0
27	3.043628589655559E-4	0.0	0.0	18.83050397619639	0.0
28	3.043628589655559E-4	0.0	0.0	19.429672700355983	0.0
29	3.043628589655559E-4	0.0	0.0	20.05611233667889	0.0
30	3.043628589655559E-4	0.0	0.0	20.84672529912782	0.0
31	3.043628589655559E-4	0.0	0.0	21.51748016771611	0.0
32	3.043628589655559E-4	0.0	0.0	22.16650352817426	0.0
33	3.65235430758667E-4	0.0	0.0	22.754715189411094	0.0
34	3.65235430758667E-4	0.0	0.0	23.38444194461083	0.0
35	3.65235430758667E-4	0.0	0.0	24.060675344660503	0.0
36	3.65235430758667E-4	0.0	0.0	24.69655022961134	0.0
37	3.65235430758667E-4	0.0	0.0	25.339547205461972	0.0
38	4.261080025517782E-4	0.0	0.0	26.0011711882813	0.0
39	4.261080025517782E-4	0.0	0.0	27.00824701602653	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	55	1.8189894E-12	45.0	9
TAAGCGC	20	7.0341723E-4	45.0	34
TACACCG	20	7.0341723E-4	45.0	41
TTTTGCG	1445	0.0	39.705883	1
TTGGGAC	3750	0.0	39.479996	5
CGTTTGG	930	0.0	39.435486	2
TACGGGT	40	3.4594268E-7	39.375	4
TCGACGT	40	3.4594268E-7	39.375	26
CGTTTTT	2105	0.0	39.334915	1
ACACGCG	310	0.0	39.193546	36
TTACACG	315	0.0	38.57143	34
TTTGGGA	9945	0.0	38.37104	4
GACCGAT	640	0.0	38.320312	9
TGGGCGA	1805	0.0	38.01939	6
TTTCGCG	610	0.0	37.991802	1
TAACGAC	30	1.1401356E-4	37.499996	28
TATCTCG	30	1.1401356E-4	37.499996	1
CGCAACA	30	1.1401356E-4	37.499996	23
CACGCGG	320	0.0	37.265625	37
TTGGGAT	6350	0.0	37.169292	5
>>END_MODULE