SRR2933884.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933884.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2181667
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	7885	0.36142087678825413	RNA PCR Primer, Index 38 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7567	0.34684486679222815	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	7410	0.3396485348130581	RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	5811	0.266355956248135	RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	5798	0.26576008162565595	RNA PCR Primer, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	3219	0.1475477238276969	RNA PCR Primer, Index 38 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	2598	0.11908325147696694	RNA PCR Primer, Index 38 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3780	0.0	40.535717	1
TACGAAT	145	0.0	38.793102	12
TGGGCGA	2365	0.0	38.625793	6
TACGGGA	275	0.0	38.454544	4
TTACGGG	405	0.0	38.333332	3
TTGTTCG	425	0.0	38.11765	1
TAACCGA	30	1.1402578E-4	37.500004	12
ACGACGG	30	1.1402578E-4	37.500004	27
TTTCGCG	590	0.0	36.991528	1
GGGCGAT	3535	0.0	36.91655	7
TTTGGGA	9080	0.0	36.77313	4
TTTGGGC	4385	0.0	36.68757	4
TGTTTCG	345	0.0	36.52174	1
TTTTGCG	1645	0.0	36.519756	1
TTGGGAC	3715	0.0	36.460297	5
CTTACGG	105	0.0	36.428574	2
TTCGGGA	1595	0.0	36.394985	4
TTGGGCA	2645	0.0	36.153122	5
GGCCGAT	750	0.0	36.0	8
CCGCGTA	25	0.0021075227	36.0	44