##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933884.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2181667
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.408440426517885	33.0	31.0	34.0	31.0	34.0
2	32.63207171396918	34.0	31.0	34.0	31.0	34.0
3	32.6788309123253	34.0	31.0	34.0	31.0	34.0
4	36.05348295592315	37.0	35.0	37.0	35.0	37.0
5	36.09554849571452	37.0	35.0	37.0	35.0	37.0
6	35.71974137207924	37.0	35.0	37.0	35.0	37.0
7	36.04187531827726	37.0	35.0	37.0	35.0	37.0
8	36.05298929671668	37.0	36.0	37.0	35.0	37.0
9	37.892439588626495	39.0	38.0	39.0	35.0	39.0
10	37.58287630513731	39.0	37.0	39.0	35.0	39.0
11	37.50744270321731	39.0	37.0	39.0	35.0	39.0
12	37.27399598563851	39.0	37.0	39.0	35.0	39.0
13	37.19737888504525	39.0	37.0	39.0	34.0	39.0
14	38.2070723900577	40.0	38.0	41.0	33.0	41.0
15	38.34021278224404	40.0	38.0	41.0	33.0	41.0
16	38.46248442131636	40.0	38.0	41.0	34.0	41.0
17	38.43561322603312	40.0	38.0	41.0	34.0	41.0
18	38.38890719802793	40.0	38.0	41.0	34.0	41.0
19	38.395824385664724	40.0	37.0	41.0	34.0	41.0
20	38.32848505294346	40.0	37.0	41.0	34.0	41.0
21	38.24583449261505	40.0	37.0	41.0	34.0	41.0
22	38.274264587583716	40.0	37.0	41.0	34.0	41.0
23	38.2400228815855	40.0	37.0	41.0	34.0	41.0
24	38.210602718013334	40.0	37.0	41.0	34.0	41.0
25	38.106064766071086	40.0	36.0	41.0	34.0	41.0
26	38.03245454049587	40.0	36.0	41.0	34.0	41.0
27	38.01594560489754	40.0	36.0	41.0	34.0	41.0
28	37.9474319408049	40.0	36.0	41.0	34.0	41.0
29	37.922810401404064	40.0	36.0	41.0	34.0	41.0
30	37.826481768299196	40.0	36.0	41.0	34.0	41.0
31	37.73453923078087	40.0	36.0	41.0	33.0	41.0
32	37.668316017064015	40.0	36.0	41.0	33.0	41.0
33	37.58448379152272	40.0	36.0	41.0	33.0	41.0
34	37.47415806353582	40.0	35.0	41.0	33.0	41.0
35	37.41157747722269	40.0	35.0	41.0	33.0	41.0
36	37.29770812869242	40.0	35.0	41.0	32.0	41.0
37	37.228388658764146	40.0	35.0	41.0	32.0	41.0
38	37.22541066074704	40.0	35.0	41.0	32.0	41.0
39	37.204335950445234	39.0	35.0	41.0	32.0	41.0
40	37.08465636598069	39.0	35.0	41.0	32.0	41.0
41	36.93185302798273	39.0	35.0	41.0	32.0	41.0
42	36.90626892188405	39.0	35.0	41.0	32.0	41.0
43	36.79630667741686	39.0	35.0	41.0	31.0	41.0
44	36.69026207940992	39.0	35.0	41.0	31.0	41.0
45	36.69287659390732	39.0	35.0	41.0	31.0	41.0
46	36.611360028822	39.0	35.0	41.0	31.0	41.0
47	36.522314358699106	39.0	35.0	41.0	31.0	41.0
48	36.449197333965266	39.0	35.0	40.0	31.0	41.0
49	36.428632784013324	38.0	35.0	40.0	31.0	41.0
50	36.34632599750558	38.0	35.0	40.0	31.0	41.0
51	35.35355074812059	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	34.0
10	49.0
11	42.0
12	34.0
13	28.0
14	34.0
15	47.0
16	95.0
17	235.0
18	400.0
19	709.0
20	1160.0
21	1899.0
22	2951.0
23	4358.0
24	6445.0
25	9409.0
26	13266.0
27	15955.0
28	18443.0
29	22005.0
30	27427.0
31	36171.0
32	48315.0
33	72762.0
34	144320.0
35	155652.0
36	155840.0
37	247451.0
38	467421.0
39	728292.0
40	410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.527712066048576	4.706676133433746	54.3523369973511	16.41327480316657
2	21.38254829907589	4.437524150110901	51.52995392972438	22.64997362108883
3	21.89710895384126	4.892726525175474	50.09811304841665	23.11205147256662
4	20.05878990698397	5.191626403112849	49.26925144854829	25.48033224135489
5	19.701906844628443	6.02497998090451	48.148365447155776	26.12474772731127
6	22.2587131766672	6.653261015544536	52.008762107141	19.079263700647257
7	82.01719144122362	2.8162868118736726	10.061618019615276	5.104903727287437
8	82.92791704691871	3.3541782499345683	8.568677071248729	5.149227631897993
9	76.60894169458491	5.718287896365486	11.240945570520157	6.431824838529436
10	40.33635747343659	24.998086325731652	19.075688452912384	15.589867747919367
11	30.475182509521385	23.499736669253373	25.687238244883385	20.337842576341853
12	27.630110369731035	20.65503122153839	30.8553046821536	20.85955372657697
13	26.284671308682768	20.97487838428138	31.85027779216535	20.890172514870507
14	20.748629373777025	23.00479404052039	34.32146152460481	21.925115061097774
15	19.222090264004542	22.9236634188444	35.49455531022837	22.359691006922688
16	24.161707538318176	23.29663509600686	30.62813894146082	21.913518424214146
17	23.54048532612906	24.115458500311917	29.165954290916073	23.178101882642952
18	24.62410624536192	23.000210389578243	30.585602660717697	21.790080704342138
19	24.039094875615756	25.257245950000616	28.844686196381026	21.8589729780026
20	25.099751703628463	27.558422068995863	28.11015613290204	19.23167009447363
21	24.61750578800523	26.2796292926464	29.96965164711205	19.133213272236322
22	23.219400577631692	23.393808495980366	31.137015869057926	22.249775057330016
23	22.70076047352781	24.125496695875217	30.233486595342	22.940256235254967
24	23.07515308248234	24.311776270164053	29.207665514489605	23.405405132863997
25	23.186627473395344	25.24441172736261	27.032677305931657	24.53628349331039
26	21.491593354989554	25.32416725375596	27.988414363878633	25.195825027375857
27	21.180088436961277	25.066657743826166	30.128154296691473	23.625099522521083
28	19.979401072665993	25.06427424533625	29.066443228962076	25.889881453035684
29	21.75863685887901	24.95321238300804	28.360836002928036	24.927314755184913
30	24.036528031088153	24.42985111843375	29.514403435538057	22.019217414940044
31	23.858040663400967	26.457337439673424	28.013211915475644	21.671409981449965
32	25.24766611953153	25.43133301278334	26.59237179642906	22.728629071256062
33	25.49096631154067	24.393411093443685	27.1147704943055	23.000852100710144
34	22.200775828758466	24.0404699708984	30.10486934990537	23.653884850437763
35	22.16451914980609	26.286596442078462	28.18482380674961	23.364060601365836
36	24.899675340003768	27.16697827853655	26.471500921084655	21.461845460375027
37	23.224259247630368	27.80158475147674	26.97386906434392	22.000286936548978
38	22.901982749888045	25.475886099941008	28.274525855687415	23.347605294483532
39	22.23680332516374	23.826046779824786	28.835702240534417	25.10144765447706
40	23.199690878580462	23.571745825554498	30.40661109142688	22.821952204438166
41	21.38286915464184	25.128262012488616	28.71327292386968	24.77559590899986
42	22.787437312843803	23.811104077753388	29.45733698130833	23.944121628094482
43	22.976467077697926	22.857292153202117	29.350721260394003	24.815519508705957
44	22.81489338198726	22.770340294829598	28.083204265362223	26.331562057820925
45	22.799813170387598	22.411807118134895	27.169178430988783	27.619201280488724
46	22.53125706168723	23.578346282911188	28.985220934267236	24.90517572113434
47	20.543144302040595	24.481004662948102	30.964395574576685	24.011455460434615
48	20.535718787514316	24.854159686148254	30.688093095784097	23.922028430553333
49	23.480943700390572	22.333610033061873	30.722103785774824	23.46334248077273
50	21.53642146120375	22.97600871260371	29.43391452499396	26.05365530119858
51	20.270646253529982	22.458697867273052	28.657352382375496	28.61330349682147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	389.0
1	1317.5
2	2246.0
3	7180.0
4	12114.0
5	7845.5
6	3577.0
7	3851.0
8	4125.0
9	4061.0
10	3997.0
11	3995.5
12	3994.0
13	3940.0
14	3886.0
15	3912.0
16	3938.0
17	4074.5
18	4211.0
19	4637.0
20	5063.0
21	5657.0
22	6251.0
23	7400.0
24	8549.0
25	9796.0
26	13322.0
27	15601.0
28	19565.0
29	23529.0
30	27388.5
31	31248.0
32	35622.0
33	39996.0
34	47489.5
35	54983.0
36	58443.5
37	61904.0
38	67391.0
39	72878.0
40	81677.0
41	90476.0
42	100572.5
43	110669.0
44	123399.5
45	136130.0
46	158386.0
47	180642.0
48	210603.0
49	240564.0
50	238959.5
51	237355.0
52	208342.5
53	179330.0
54	159679.0
55	140028.0
56	129887.0
57	119746.0
58	113447.0
59	107148.0
60	94852.5
61	82557.0
62	71805.5
63	61054.0
64	52072.0
65	43090.0
66	35711.5
67	28333.0
68	23884.5
69	19436.0
70	16075.0
71	12714.0
72	10712.0
73	8710.0
74	6836.0
75	3972.5
76	2983.0
77	2092.5
78	1202.0
79	889.0
80	576.0
81	421.5
82	267.0
83	189.0
84	111.0
85	68.5
86	26.0
87	22.0
88	18.0
89	11.0
90	4.0
91	7.0
92	10.0
93	6.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2181667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.272924345544403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1087847179365	19.539074419427337
2	12.394934443067442	7.008820875536567
3	5.0201247338278625	4.258008204141341
4	2.5215912440380945	2.85171033892305
5	1.4962153600351706	2.115119183945793
6	0.9901620345811629	1.6796865776126122
7	0.7129321069807001	1.4109672866922431
8	0.5675204218757189	1.2836369561794914
9	0.45967981634450095	1.169684340361364
>10	6.17206114150456	42.90888314486016
>50	0.47218098050457996	8.17901677988213
>100	0.07543200675708081	3.8248776246091207
>500	0.005587328364438387	1.1052797052157908
>1k	0.001971998246272372	1.0636499344867416
>5k	8.216659359468216E-4	1.6015846281261725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	7885	0.36142087678825413	RNA PCR Primer, Index 38 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7567	0.34684486679222815	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	7410	0.3396485348130581	RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	5811	0.266355956248135	RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	5798	0.26576008162565595	RNA PCR Primer, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	3219	0.1475477238276969	RNA PCR Primer, Index 38 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	2598	0.11908325147696694	RNA PCR Primer, Index 38 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2918254710732665E-4	0.0	0.0	0.3786554043307251	0.0
2	2.2918254710732665E-4	0.0	0.0	1.31472859973589	0.0
3	2.2918254710732665E-4	0.0	0.0	1.9924672280416764	0.0
4	2.2918254710732665E-4	0.0	0.0	2.7236512263328914	0.0
5	2.2918254710732665E-4	0.0	0.0	3.958349280618903	0.0
6	2.2918254710732665E-4	0.0	0.0	4.935721171012808	0.0
7	2.2918254710732665E-4	0.0	0.0	5.781633952385951	0.0
8	2.2918254710732665E-4	0.0	0.0	7.01032742393775	0.0
9	2.2918254710732665E-4	0.0	0.0	7.66688958489082	0.0
10	2.2918254710732665E-4	0.0	0.0	8.727500576394107	0.0
11	2.2918254710732665E-4	0.0	0.0	10.755078570652625	0.0
12	2.2918254710732665E-4	0.0	0.0	12.479218872541043	0.0
13	2.75019056528792E-4	0.0	0.0	13.07440594737877	0.0
14	2.75019056528792E-4	0.0	0.0	13.33869926070294	0.0
15	3.208555659502573E-4	0.0	0.0	13.673168269951372	0.0
16	3.208555659502573E-4	0.0	0.0	14.369470684572851	0.0
17	3.208555659502573E-4	0.0	0.0	15.284596595172408	0.0
18	3.208555659502573E-4	0.0	0.0	16.218377965106498	0.0
19	3.208555659502573E-4	0.0	0.0	16.93947793132499	0.0
20	3.6669207537172265E-4	0.0	0.0	17.754771924404594	0.0
21	3.6669207537172265E-4	0.0	0.0	18.857414995047364	0.0
22	3.6669207537172265E-4	0.0	0.0	20.052968670287445	0.0
23	4.1252858479318795E-4	0.0	0.0	21.1370021181051	0.0
24	4.1252858479318795E-4	0.0	0.0	21.988552790137085	0.0
25	4.583650942146533E-4	0.0	0.0	22.776299041054386	0.0
26	4.583650942146533E-4	0.0	0.0	23.442853561061334	0.0
27	4.583650942146533E-4	0.0	0.0	24.13397645011819	0.0
28	4.583650942146533E-4	0.0	0.0	24.807268937010093	0.0
29	4.583650942146533E-4	0.0	0.0	25.54532841171453	0.0
30	4.583650942146533E-4	0.0	0.0	26.469804970236062	0.0
31	4.583650942146533E-4	0.0	0.0	27.199934728810582	0.0
32	5.042016036361186E-4	0.0	0.0	27.920713839463126	0.0
33	5.042016036361186E-4	0.0	0.0	28.638605250022117	0.0
34	5.042016036361186E-4	0.0	0.0	29.417367545092812	0.0
35	5.50038113057584E-4	0.0	0.0	30.241370474962494	0.0
36	5.50038113057584E-4	0.0	0.0	30.94409000090298	0.0
37	5.50038113057584E-4	0.0	0.0	31.6667484084418	0.0
38	5.958746224790492E-4	0.0	0.0	32.46540374860141	0.0
39	5.958746224790492E-4	0.0	0.0	33.7743569481502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3780	0.0	40.535717	1
TACGAAT	145	0.0	38.793102	12
TGGGCGA	2365	0.0	38.625793	6
TACGGGA	275	0.0	38.454544	4
TTACGGG	405	0.0	38.333332	3
TTGTTCG	425	0.0	38.11765	1
TAACCGA	30	1.1402578E-4	37.500004	12
ACGACGG	30	1.1402578E-4	37.500004	27
TTTCGCG	590	0.0	36.991528	1
GGGCGAT	3535	0.0	36.91655	7
TTTGGGA	9080	0.0	36.77313	4
TTTGGGC	4385	0.0	36.68757	4
TGTTTCG	345	0.0	36.52174	1
TTTTGCG	1645	0.0	36.519756	1
TTGGGAC	3715	0.0	36.460297	5
CTTACGG	105	0.0	36.428574	2
TTCGGGA	1595	0.0	36.394985	4
TTGGGCA	2645	0.0	36.153122	5
GGCCGAT	750	0.0	36.0	8
CCGCGTA	25	0.0021075227	36.0	44
>>END_MODULE