##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1400004
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50600355427556	33.0	31.0	34.0	31.0	34.0
2	32.74113716817952	34.0	31.0	34.0	31.0	34.0
3	32.78521061368396	34.0	31.0	34.0	31.0	34.0
4	36.14187745177871	37.0	35.0	37.0	35.0	37.0
5	36.166051668423805	37.0	35.0	37.0	35.0	37.0
6	35.79349273287791	37.0	35.0	37.0	35.0	37.0
7	36.091516167096664	37.0	35.0	37.0	35.0	37.0
8	36.10607326836209	37.0	37.0	37.0	35.0	37.0
9	37.9597358293262	39.0	38.0	39.0	35.0	39.0
10	37.70612369678944	39.0	37.0	39.0	35.0	39.0
11	37.63612818249091	39.0	37.0	39.0	35.0	39.0
12	37.33905903125991	39.0	37.0	39.0	35.0	39.0
13	37.21391081739767	39.0	37.0	39.0	34.0	39.0
14	38.14835386184611	40.0	38.0	41.0	33.0	41.0
15	38.29566486952894	40.0	38.0	41.0	33.0	41.0
16	38.47614435387327	40.0	38.0	41.0	34.0	41.0
17	38.49019645658155	40.0	38.0	41.0	34.0	41.0
18	38.4350123285362	40.0	37.0	41.0	34.0	41.0
19	38.429084488330034	40.0	37.0	41.0	34.0	41.0
20	38.309239116459665	40.0	37.0	41.0	34.0	41.0
21	38.2419078802632	40.0	37.0	41.0	34.0	41.0
22	38.29823343361876	40.0	37.0	41.0	34.0	41.0
23	38.25912783106334	40.0	36.0	41.0	34.0	41.0
24	38.22455292984877	40.0	36.0	41.0	34.0	41.0
25	38.12952891563167	40.0	36.0	41.0	34.0	41.0
26	38.05277699206574	40.0	36.0	41.0	34.0	41.0
27	38.02595206870838	40.0	36.0	41.0	34.0	41.0
28	37.942393021734226	40.0	36.0	41.0	34.0	41.0
29	37.84958828689061	40.0	35.0	41.0	34.0	41.0
30	37.7681285196328	40.0	35.0	41.0	34.0	41.0
31	37.642397450293	40.0	35.0	41.0	33.0	41.0
32	37.4912078822632	40.0	35.0	41.0	33.0	41.0
33	37.39938100176857	40.0	35.0	41.0	33.0	41.0
34	37.272142793877734	40.0	35.0	41.0	33.0	41.0
35	37.15693312304822	39.0	35.0	41.0	32.0	41.0
36	37.08385261756395	39.0	35.0	41.0	32.0	41.0
37	36.99692358021834	39.0	35.0	41.0	32.0	41.0
38	36.907988834317614	39.0	35.0	41.0	32.0	41.0
39	36.8409740257885	39.0	35.0	41.0	32.0	41.0
40	36.758265690669454	39.0	35.0	41.0	31.0	41.0
41	36.639071031225626	39.0	35.0	41.0	31.0	41.0
42	36.66174382358908	39.0	35.0	41.0	31.0	41.0
43	36.57524407073122	39.0	35.0	41.0	31.0	41.0
44	36.47980005771412	39.0	35.0	41.0	31.0	41.0
45	36.46666509524258	39.0	35.0	41.0	31.0	41.0
46	36.41938165890954	38.0	35.0	41.0	31.0	41.0
47	36.333580475484354	38.0	35.0	41.0	31.0	41.0
48	36.249930714483675	38.0	35.0	40.0	31.0	41.0
49	36.22094079731201	38.0	35.0	40.0	31.0	41.0
50	36.1353124705358	38.0	35.0	40.0	31.0	41.0
51	35.15849311859109	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	25.0
10	26.0
11	20.0
12	17.0
13	14.0
14	28.0
15	43.0
16	94.0
17	169.0
18	229.0
19	443.0
20	739.0
21	1256.0
22	1928.0
23	2864.0
24	4317.0
25	6893.0
26	9782.0
27	11298.0
28	12598.0
29	14223.0
30	17322.0
31	22560.0
32	30941.0
33	48659.0
34	100541.0
35	103071.0
36	99986.0
37	158742.0
38	293962.0
39	456884.0
40	327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.67936091611167	4.600915425955926	54.57541549881286	17.144308159119547
2	20.8969402944563	5.111128253919275	52.90449170145229	21.087439750172145
3	21.037725606498267	4.990057171265224	51.801923423075934	22.17029379916057
4	19.51515852811849	4.927200207999405	51.22435364470387	24.333287619178233
5	19.54972985791469	6.033268476375782	49.48878717489379	24.92821449081574
6	22.05136556752695	6.750909283116334	52.33463618675375	18.863088962602966
7	82.46905008842832	2.5605641126739638	10.347256150696712	4.6231296482010045
8	84.28940203027992	3.319633372476079	8.19333373333219	4.197630863911817
9	78.0985625755355	5.532341336167611	10.505541412738822	5.86355467555807
10	41.030668483804334	28.237062179822342	16.968094376873207	13.764174959500117
11	33.44140445313013	22.58086405467413	25.35999897143151	18.61773252076423
12	30.207056551266998	20.122013937103038	28.979631486767182	20.691298024862785
13	27.540207027979918	20.150728140776742	31.67919520229942	20.62986962894392
14	21.092582592621163	22.036722752220708	34.25533069905515	22.615363956102982
15	19.210087971177224	22.97579149773858	36.17789663458104	21.636223896503154
16	25.60764112102537	22.87022037079894	30.21734223616504	21.304796272010652
17	26.095639726743634	23.80214627958206	28.16577666920952	21.936437324464787
18	26.4497101436853	22.235150756712123	30.328484775757786	20.98665432384479
19	26.014997100008287	24.52435850183285	27.704920843083304	21.755723555075555
20	24.965571526938493	27.8347776149211	28.239490744312157	18.960160113828245
21	25.19121373938932	25.51906994551444	30.55419841657595	18.73551789852029
22	23.749146431010196	22.124079645486727	31.922908791689164	22.20386513181391
23	22.553864131816766	23.73786074896929	31.38655318127663	22.32172193793732
24	23.1784337759035	24.712786534895613	29.82012908534547	22.288650603855416
25	24.139145316727667	25.68535518469947	26.762494964300103	23.413004534272762
26	21.872437507321408	25.48649861000397	27.092422593078307	25.548641289596315
27	21.889794600586857	24.234002188565178	30.390627455350128	23.48557575549784
28	20.160728112205394	25.257499264287812	29.112488250033568	25.469284373473215
29	22.14336530467056	25.91835451898709	28.201847994720016	23.736432181622337
30	25.869497515721385	24.096716866523238	28.591775452070138	21.44201016568524
31	25.343213305104843	24.81671480938626	28.658060976968635	21.18201090854026
32	26.657852406135984	24.32964477244351	27.19527944205874	21.81722337936177
33	27.301493424304503	23.385218899374575	26.71128082491193	22.602006851408994
34	22.879577486921466	24.432644478158632	30.19627086779752	22.49150716712238
35	22.36957894406016	27.046422724506503	28.603132562478393	21.980865768954946
36	25.78106919694515	26.892280307770548	25.889140316741955	21.437510178542347
37	23.96000297142008	27.28835060471256	27.040279884914614	21.711366538952745
38	24.627286779180633	25.06085696898009	28.163633818189094	22.14822243365019
39	23.406147411007396	24.0718597946863	27.527421350224714	24.99457144408159
40	25.433998760003547	23.576789780600627	28.554918414518816	22.434293044877016
41	21.320224799357714	24.606072554078416	27.97513435675898	26.098568289804884
42	21.459724400787426	22.868363233247905	30.241842166165238	25.43007019979943
43	22.207650835283328	22.741006454267275	29.946200153713846	25.105142556735554
44	23.463290104885417	22.794577729777917	28.34013331390482	25.401998851431852
45	22.654578129776773	22.937291607738263	26.9037802749135	27.504349987571462
46	22.74400644569587	23.85307470550084	28.283062048394147	25.119856800409142
47	20.782654906700266	24.504358558975543	31.38848174719501	23.32450478712918
48	21.301867708949406	25.161785252042137	30.084056902694563	23.4522901363139
49	24.027288493461445	22.611292539164175	29.982914334530474	23.378504632843907
50	22.004865700383714	23.686075182642334	28.785489184316614	25.523569932657335
51	20.867440378741776	23.689646601009713	27.872777506349983	27.57013551389853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	869.0
2	1471.0
3	5984.0
4	10497.0
5	6861.5
6	3226.0
7	3225.5
8	3225.0
9	3331.0
10	3437.0
11	3301.5
12	3166.0
13	3012.0
14	2858.0
15	2784.0
16	2710.0
17	2849.5
18	2989.0
19	3000.0
20	3011.0
21	3490.5
22	3970.0
23	4733.5
24	5497.0
25	6820.5
26	9695.5
27	11247.0
28	13642.5
29	16038.0
30	18243.0
31	20448.0
32	24499.5
33	28551.0
34	32490.5
35	36430.0
36	39061.5
37	41693.0
38	45093.5
39	48494.0
40	52200.5
41	55907.0
42	61804.0
43	67701.0
44	73674.0
45	79647.0
46	95006.0
47	110365.0
48	122672.0
49	134979.0
50	135847.0
51	136715.0
52	122350.5
53	107986.0
54	99348.0
55	90710.0
56	86301.5
57	81893.0
58	77393.0
59	72893.0
60	65416.0
61	57939.0
62	51288.5
63	44638.0
64	38747.0
65	32856.0
66	27683.0
67	22510.0
68	19695.5
69	16881.0
70	14098.5
71	11316.0
72	9596.0
73	7876.0
74	6326.5
75	3683.0
76	2589.0
77	1932.5
78	1276.0
79	966.0
80	656.0
81	470.0
82	284.0
83	237.0
84	190.0
85	104.5
86	19.0
87	14.5
88	10.0
89	7.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	8.5
96	16.0
97	8.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1400004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.786509862601168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14585755505747	21.48973297609539
2	10.559282688314633	6.290483558749553
3	3.877977989485025	3.465342888922379
4	1.900079595516805	2.263869584463542
5	1.246436514236402	1.8563496762204403
6	0.8655370443801089	1.5468796625324868
7	0.6654091495696677	1.387415133742537
8	0.5355484586994386	1.2761695557561346
9	0.5033389491517992	1.349343951582724
>10	7.4558120029884485	49.575622868116795
>50	0.18403307035817315	3.5513225383056213
>100	0.05415890070861911	2.942802078224296
>500	0.002901369570403802	0.5680784659823294
>1k	0.0033849311654711024	1.9771838495282543
>5k	2.4178079753365018E-4	0.4594032117775455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6379	0.45564155530984196	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4244	0.3031419910228828	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	3565	0.2546421295939154	Illumina PCR Primer Index 5 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	2812	0.20085656898123147	RNA PCR Primer, Index 33 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	2338	0.1669995228585061	RNA PCR Primer, Index 33 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1990	0.14214245102156853	Illumina PCR Primer Index 5 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	1803	0.12878534632758193	Illumina PCR Primer Index 5 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1789	0.12778534918471662	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	1544	0.11028539918457375	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1449	0.10349970428655919	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	1445	0.10321399081716909	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.142836734752186E-5	0.0	0.0	0.2659278116348239	0.0
2	7.142836734752186E-5	0.0	0.0	0.9892114593958303	0.0
3	7.142836734752186E-5	0.0	0.0	1.4730672198079433	0.0
4	7.142836734752186E-5	0.0	0.0	1.982851477567207	0.0
5	7.142836734752186E-5	0.0	0.0	3.0412055965554385	0.0
6	7.142836734752186E-5	0.0	0.0	3.919060231256482	0.0
7	7.142836734752186E-5	0.0	0.0	4.680843769017803	0.0
8	7.142836734752186E-5	0.0	0.0	5.745697869434658	0.0
9	7.142836734752186E-5	0.0	0.0	6.399910285970612	0.0
10	7.142836734752186E-5	0.0	0.0	7.457407264550673	0.0
11	7.142836734752186E-5	0.0	0.0	8.990760026399924	0.0
12	7.142836734752186E-5	0.0	0.0	10.375184642329593	0.0
13	7.142836734752186E-5	0.0	0.0	10.845611869680372	0.0
14	7.142836734752186E-5	0.0	0.0	11.082182622335365	0.0
15	7.142836734752186E-5	0.0	0.0	11.364610386827467	0.0
16	7.142836734752186E-5	0.0	0.0	11.86918037377036	0.0
17	7.142836734752186E-5	0.0	0.0	12.529178488061463	0.0
18	7.142836734752186E-5	0.0	0.0	13.216033668475234	0.0
19	7.142836734752186E-5	0.0	0.0	13.764317816234811	0.0
20	7.142836734752186E-5	0.0	0.0	14.374887500321428	0.0
21	7.142836734752186E-5	0.0	0.0	15.221742223593647	0.0
22	7.142836734752186E-5	0.0	0.0	16.119953942988733	0.0
23	7.142836734752186E-5	0.0	0.0	16.910808826260496	0.0
24	7.142836734752186E-5	0.0	0.0	17.518378518918517	0.0
25	7.142836734752186E-5	0.0	0.0	18.089448315861954	0.0
26	7.142836734752186E-5	0.0	0.0	18.600018285662042	0.0
27	7.142836734752186E-5	0.0	0.0	19.21508795689155	0.0
28	1.4285673469504372E-4	0.0	0.0	19.755657840977598	0.0
29	1.4285673469504372E-4	0.0	0.0	20.35908468832946	0.0
30	1.4285673469504372E-4	0.0	0.0	21.10765397813149	0.0
31	1.4285673469504372E-4	0.0	0.0	21.679009488544317	0.0
32	1.4285673469504372E-4	0.0	0.0	22.23500789997743	0.0
33	1.4285673469504372E-4	0.0	0.0	22.787720606512554	0.0
34	1.4285673469504372E-4	0.0	0.0	23.379718915088812	0.0
35	1.4285673469504372E-4	0.0	0.0	24.02607421121654	0.0
36	1.4285673469504372E-4	0.0	0.0	24.57678692346593	0.0
37	1.4285673469504372E-4	0.0	0.0	25.15907097408293	0.0
38	1.4285673469504372E-4	0.0	0.0	25.864497530007057	0.0
39	1.4285673469504372E-4	0.0	0.0	27.196136582466906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	20	7.033813E-4	45.000004	3
GCGATCG	30	2.1658416E-6	45.000004	9
TCGACAC	20	7.033813E-4	45.000004	20
TTATGCG	50	2.1827873E-11	45.0	1
CGTTTTT	3105	0.0	43.4058	1
TATCCGG	50	1.0822987E-9	40.5	2
CGTTAGG	95	0.0	40.263157	2
TTGGGAT	3630	0.0	38.367767	5
TGGGCGA	1555	0.0	38.19936	6
TTTGGGA	7045	0.0	37.973743	4
TCGTTAG	60	1.5643309E-10	37.500004	1
TTTGGGC	2400	0.0	37.5	4
TTTTGCG	1190	0.0	37.2479	1
GGGCGAT	2330	0.0	37.178112	7
TATTGCG	85	0.0	37.058823	1
TTGGGAG	4150	0.0	36.759037	5
TAATCGT	80	0.0	36.562504	21
TTGTTCG	340	0.0	36.397057	1
GACCGAT	650	0.0	36.346153	9
TCTGGGA	3465	0.0	36.23377	4
>>END_MODULE