SRR2933881.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361463
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1618	0.44762534477941035	RNA PCR Primer, Index 41 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1192	0.32977095857667316	RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1064	0.2943593120181042	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	981	0.27139707245278216	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	412	0.11398123736039373	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	397	0.10983143502931143	RNA PCR Primer, Index 41 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	30	2.1613578E-6	45.000004	2
CGGATGT	45	3.8380676E-10	45.000004	21
GGTGCAA	30	2.1613578E-6	45.000004	8
CGATGTG	30	2.1613578E-6	45.000004	10
CGGTAGT	45	3.8380676E-10	45.000004	12
TTTCTCG	65	0.0	45.000004	1
ATTAGTC	35	1.2088458E-7	45.0	31
TCATTCG	20	7.0268626E-4	45.0	29
GGGTACG	20	7.0268626E-4	45.0	7
CACGCTA	20	7.0268626E-4	45.0	19
TTATCGG	20	7.0268626E-4	45.0	31
ACGGGTC	25	3.885482E-5	45.0	5
ATACTAG	20	7.0268626E-4	45.0	15
AGTGATC	25	3.885482E-5	45.0	30
AACGACG	20	7.0268626E-4	45.0	28
GCGATCT	75	0.0	45.0	9
TCTCCTA	25	3.885482E-5	45.0	34
CGTCATA	20	7.0268626E-4	45.0	38
ACAAGCC	20	7.0268626E-4	45.0	19
ATTTCGC	20	7.0268626E-4	45.0	11