##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361463
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49682816775161	33.0	31.0	34.0	31.0	34.0
2	32.70763259310082	34.0	31.0	34.0	31.0	34.0
3	32.76154405845135	34.0	31.0	34.0	31.0	34.0
4	36.11371841654609	37.0	35.0	37.0	35.0	37.0
5	36.15235584278336	37.0	35.0	37.0	35.0	37.0
6	35.78833241576593	37.0	35.0	37.0	35.0	37.0
7	36.102801669880456	37.0	35.0	37.0	35.0	37.0
8	36.1066969510019	37.0	37.0	37.0	35.0	37.0
9	37.95812849447938	39.0	38.0	39.0	35.0	39.0
10	37.62487446847948	39.0	37.0	39.0	35.0	39.0
11	37.525478403045405	39.0	37.0	39.0	35.0	39.0
12	37.27566030271425	39.0	37.0	39.0	35.0	39.0
13	37.142869394654504	39.0	37.0	39.0	34.0	39.0
14	38.28604034161173	40.0	38.0	41.0	33.0	41.0
15	38.440197198606775	40.0	38.0	41.0	34.0	41.0
16	38.54803119544739	40.0	38.0	41.0	35.0	41.0
17	38.563717448258885	40.0	38.0	41.0	35.0	41.0
18	38.53594143798951	40.0	37.0	41.0	35.0	41.0
19	38.53509211177908	40.0	37.0	41.0	35.0	41.0
20	38.45587238527872	40.0	37.0	41.0	34.0	41.0
21	38.36338989052822	40.0	37.0	41.0	34.0	41.0
22	38.35935074959263	40.0	37.0	41.0	34.0	41.0
23	38.33637467735287	40.0	37.0	41.0	34.0	41.0
24	38.304822346962204	40.0	37.0	41.0	34.0	41.0
25	38.21847879312682	40.0	37.0	41.0	34.0	41.0
26	38.15008175110592	40.0	36.0	41.0	34.0	41.0
27	38.10081253129643	40.0	36.0	41.0	34.0	41.0
28	38.046497151852336	40.0	36.0	41.0	34.0	41.0
29	38.04326860563875	40.0	36.0	41.0	34.0	41.0
30	37.987531227262544	40.0	36.0	41.0	34.0	41.0
31	37.91362601428085	40.0	36.0	41.0	34.0	41.0
32	37.83941371592667	40.0	36.0	41.0	34.0	41.0
33	37.75208804220625	40.0	36.0	41.0	34.0	41.0
34	37.72774253519724	40.0	36.0	41.0	33.0	41.0
35	37.66839482879299	40.0	36.0	41.0	33.0	41.0
36	37.56813560447404	40.0	35.0	41.0	33.0	41.0
37	37.473118963766694	40.0	35.0	41.0	33.0	41.0
38	37.44087223311929	40.0	35.0	41.0	33.0	41.0
39	37.433554748342154	40.0	35.0	41.0	33.0	41.0
40	37.359323084243755	40.0	35.0	41.0	33.0	41.0
41	37.27982117118488	39.0	35.0	41.0	33.0	41.0
42	37.24395581290478	39.0	35.0	41.0	33.0	41.0
43	37.133546172084	39.0	35.0	41.0	33.0	41.0
44	37.02219314286663	39.0	35.0	41.0	32.0	41.0
45	37.01782201774456	39.0	35.0	41.0	32.0	41.0
46	36.9498759209103	39.0	35.0	41.0	32.0	41.0
47	36.89562693830351	39.0	35.0	41.0	32.0	41.0
48	36.85086717036045	39.0	35.0	41.0	32.0	41.0
49	36.84659010742455	39.0	35.0	41.0	32.0	41.0
50	36.742701189333346	39.0	35.0	41.0	32.0	41.0
51	35.83646458973671	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	4.0
11	11.0
12	4.0
13	3.0
14	1.0
15	10.0
16	11.0
17	18.0
18	42.0
19	93.0
20	160.0
21	278.0
22	417.0
23	584.0
24	905.0
25	1267.0
26	1768.0
27	2197.0
28	2598.0
29	3134.0
30	3882.0
31	5252.0
32	6916.0
33	10470.0
34	21960.0
35	32470.0
36	23839.0
37	37537.0
38	74441.0
39	131090.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.71290284206129	4.653035027098209	54.2641985486758	16.369863582164704
2	19.843801440258062	4.450524673341393	53.26326622641875	22.442407659981797
3	21.024281876706606	4.635329203818925	52.363589081040104	21.976799838434363
4	18.798051252825324	5.132198869593845	52.525154718463575	23.544595159117254
5	18.409353101147282	6.0628612057112345	49.93291152898084	25.594874164160647
6	21.697379814808155	6.7077404879614235	53.404082852186804	18.190796845043614
7	85.15505044776367	2.1412980028384645	7.947701424488813	4.755950124909051
8	86.13993686767387	2.923950722480586	6.26094510364823	4.675167306197315
9	80.17584095744239	5.090424192794283	9.286704309984701	5.447030539778622
10	40.96684861244443	23.61569510572313	18.767896022552794	16.64956025927965
11	31.288956269383032	26.18691263006172	24.221566246061148	18.302564854494097
12	27.693014222755856	22.939830632734193	29.080154815292296	20.28700032921765
13	24.26029773448458	27.423830378212983	29.144338424679706	19.17153346262273
14	19.1684902742466	28.41646309580787	31.176358299466333	21.238688330479192
15	17.495843281331698	26.292870916248688	35.90906953132132	20.302216271098285
16	19.88225627519276	25.244630847417298	33.07143469732725	21.801678180062687
17	20.96673795104893	25.02386136340372	28.071199541861823	25.938201143685525
18	22.065605608319526	24.474980841745904	31.421749943977666	22.037663605956904
19	23.675452259290715	26.021750497284646	29.38198377150635	20.920813471918287
20	25.48587268959755	27.100145796388563	28.06926296744065	19.34471854657323
21	24.27717359729765	26.51391705375101	29.415458843643748	19.793450505307597
22	22.3903968040989	25.423072347653843	28.548980116913764	23.637550731333498
23	20.754821378674997	26.155927439322973	29.11307658045222	23.97617460154981
24	21.58616511233515	25.244077540439825	30.92820012006761	22.24155722715741
25	20.48287099924474	26.66773639348979	29.271875682988302	23.577516924277173
26	20.830347781100695	28.073412769771732	28.154195588483468	22.942043860644105
27	20.532115320240248	28.058473481379835	29.699581976578514	21.709829221801403
28	19.633821442305297	27.062520921920086	30.903301306081122	22.400356329693498
29	19.859570689116175	25.612026680462453	31.407363962563252	23.12103866785812
30	21.33053728874048	27.070820526582253	29.583110857819474	22.015531326857797
31	23.14234098649101	28.45215139585518	26.740772914516835	21.664734703136972
32	24.114501345919223	27.00248711486376	27.114531777802988	21.76847976141403
33	23.39796881008568	26.452499979250987	27.254518443104825	22.895012767558505
34	21.489613044765303	26.176123144000908	28.453258009810135	23.881005801423658
35	21.16122535363232	26.62983486553257	28.62173998445207	23.587199796383032
36	25.099941072806896	25.38655408714032	28.636402619355234	20.877102220697555
37	22.575477988065167	28.14312944893392	28.75038385671563	20.53100870628529
38	21.038114551143547	28.914162722049007	27.91267709281448	22.135045633992966
39	20.363633345598306	27.73229901815677	28.091671900028498	23.812395736216434
40	22.38818357618899	25.787701645811605	28.365835507368665	23.45827927063074
41	20.642223408758294	25.23965108462	28.57387893090026	25.54424657572144
42	22.543939490348944	24.048104508621908	28.392947549265067	25.015008451764082
43	23.626484591783946	23.547085040515903	29.01016148264138	23.816268885058776
44	21.53387760296351	25.265656512561453	28.301098591003782	24.899367293471254
45	20.89923449979666	26.989484400893037	27.400591485158927	24.71068961415138
46	22.7082716626598	26.014834160066176	28.813737505636816	22.463156671637208
47	20.145630396472114	26.208491602183347	30.784616959412165	22.86126104193237
48	20.95290527661199	25.2211153008745	30.393152272846734	23.43282714966677
49	22.538683074062906	23.650000138326742	30.713517012806292	23.09779977480406
50	21.831003449869005	25.55448275480478	28.339000118961	24.275513676365215
51	20.08642654988201	25.36414515455247	26.989207747404297	27.560220548161222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	153.0
2	257.0
3	545.0
4	833.0
5	609.0
6	385.0
7	418.5
8	452.0
9	476.5
10	501.0
11	543.0
12	585.0
13	555.5
14	526.0
15	572.5
16	619.0
17	635.5
18	652.0
19	651.5
20	651.0
21	804.0
22	957.0
23	1136.0
24	1315.0
25	1586.0
26	2514.5
27	3172.0
28	3696.0
29	4220.0
30	4983.5
31	5747.0
32	6776.5
33	7806.0
34	9237.0
35	10668.0
36	12063.0
37	13458.0
38	14081.5
39	14705.0
40	17427.5
41	20150.0
42	23279.5
43	26409.0
44	28586.5
45	30764.0
46	32560.0
47	34356.0
48	37179.5
49	40003.0
50	39188.5
51	38374.0
52	31765.5
53	25157.0
54	22360.0
55	19563.0
56	17542.5
57	15522.0
58	13503.5
59	11485.0
60	10345.0
61	9205.0
62	7887.5
63	6570.0
64	5872.0
65	5174.0
66	4310.0
67	3446.0
68	2779.0
69	2112.0
70	1799.0
71	1486.0
72	1226.0
73	966.0
74	777.5
75	528.0
76	467.0
77	329.5
78	192.0
79	111.0
80	30.0
81	24.5
82	19.0
83	12.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.733279260737987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63876107884501	20.00949969450265
2	12.453011157121926	7.156316944293405
3	4.568605041339908	3.9381301345450503
4	2.228627698384003	2.561431281035332
5	1.3172866814386743	1.8924983042114114
6	0.8266199283842663	1.4250900746853816
7	0.6192115892331312	1.245438566044466
8	0.5414355082439153	1.2445774128041618
9	0.4018387981330794	1.0391531764101378
>10	7.012734920021766	50.29191779499532
>50	0.3460001445354986	5.828368446238079
>100	0.04187898003023326	1.9765970560190977
>500	9.971185721484109E-4	0.28106127148206045
>1k	0.002991355716445233	1.1099198427334376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1618	0.44762534477941035	RNA PCR Primer, Index 41 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1192	0.32977095857667316	RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1064	0.2943593120181042	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	981	0.27139707245278216	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	412	0.11398123736039373	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTG	397	0.10983143502931143	RNA PCR Primer, Index 41 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34692347487847996	0.0
2	0.0	0.0	0.0	1.4338950321333028	0.0
3	0.0	0.0	0.0	2.00407787242401	0.0
4	0.0	0.0	0.0	2.5950097243701293	0.0
5	0.0	0.0	0.0	3.594282125694746	0.0
6	0.0	0.0	0.0	4.244141170742234	0.0
7	0.0	0.0	0.0	4.830093259891054	0.0
8	0.0	0.0	0.0	5.9239811543643475	0.0
9	0.0	0.0	0.0	6.484481122549196	0.0
10	0.0	0.0	0.0	7.446128649405333	0.0
11	0.0	0.0	0.0	9.10881611672564	0.0
12	0.0	0.0	0.0	10.546584297701287	0.0
13	0.0	0.0	0.0	10.993932988991958	0.0
14	0.0	0.0	0.0	11.159095121769033	0.0
15	0.0	0.0	0.0	11.42910892677812	0.0
16	0.0	0.0	0.0	12.047152820620644	0.0
17	0.0	0.0	0.0	12.914738161305584	0.0
18	0.0	0.0	0.0	13.95633854640724	0.0
19	0.0	0.0	0.0	14.58212873793445	0.0
20	0.0	0.0	0.0	15.317750364490971	0.0
21	0.0	0.0	0.0	16.350774491441726	0.0
22	0.0	0.0	0.0	17.326531346223543	0.0
23	0.0	0.0	0.0	18.21514235205263	0.0
24	0.0	0.0	0.0	18.930568273931218	0.0
25	0.0	0.0	0.0	19.57544755618141	0.0
26	0.0	0.0	0.0	20.11851835457571	0.0
27	0.0	0.0	0.0	20.65716269715019	0.0
28	0.0	0.0	0.0	21.254180925848566	0.0
29	0.0	0.0	0.0	21.8644785220064	0.0
30	0.0	0.0	0.0	22.553622362454803	0.0
31	0.0	0.0	0.0	23.164473265590114	0.0
32	0.0	0.0	0.0	23.74461563147542	0.0
33	0.0	0.0	0.0	24.339420632263884	0.0
34	0.0	0.0	0.0	24.92647933536766	0.0
35	0.0	0.0	0.0	25.587681173453436	0.0
36	2.7665348873881973E-4	0.0	0.0	26.178889678888297	0.0
37	2.7665348873881973E-4	0.0	0.0	26.785590779692527	0.0
38	2.7665348873881973E-4	0.0	0.0	27.386758810721982	0.0
39	2.7665348873881973E-4	0.0	0.0	28.16526172803302	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	30	2.1613578E-6	45.000004	2
CGGATGT	45	3.8380676E-10	45.000004	21
GGTGCAA	30	2.1613578E-6	45.000004	8
CGATGTG	30	2.1613578E-6	45.000004	10
CGGTAGT	45	3.8380676E-10	45.000004	12
TTTCTCG	65	0.0	45.000004	1
ATTAGTC	35	1.2088458E-7	45.0	31
TCATTCG	20	7.0268626E-4	45.0	29
GGGTACG	20	7.0268626E-4	45.0	7
CACGCTA	20	7.0268626E-4	45.0	19
TTATCGG	20	7.0268626E-4	45.0	31
ACGGGTC	25	3.885482E-5	45.0	5
ATACTAG	20	7.0268626E-4	45.0	15
AGTGATC	25	3.885482E-5	45.0	30
AACGACG	20	7.0268626E-4	45.0	28
GCGATCT	75	0.0	45.0	9
TCTCCTA	25	3.885482E-5	45.0	34
CGTCATA	20	7.0268626E-4	45.0	38
ACAAGCC	20	7.0268626E-4	45.0	19
ATTTCGC	20	7.0268626E-4	45.0	11
>>END_MODULE