##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933879.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457891
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.439148181554124	33.0	31.0	34.0	31.0	34.0
2	32.66686394797015	34.0	31.0	34.0	31.0	34.0
3	32.73343219237766	34.0	31.0	34.0	31.0	34.0
4	36.0871408260918	37.0	35.0	37.0	35.0	37.0
5	36.12126029994038	37.0	35.0	37.0	35.0	37.0
6	35.771292731239534	37.0	35.0	37.0	35.0	37.0
7	36.06422489195027	37.0	35.0	37.0	35.0	37.0
8	36.06861895079833	37.0	36.0	37.0	35.0	37.0
9	37.92311488978818	39.0	38.0	39.0	35.0	39.0
10	37.6240502652378	39.0	37.0	39.0	35.0	39.0
11	37.52580199217718	39.0	37.0	39.0	35.0	39.0
12	37.28271138764466	39.0	37.0	39.0	35.0	39.0
13	37.157480710474765	39.0	37.0	39.0	34.0	39.0
14	38.36131743144111	40.0	38.0	41.0	34.0	41.0
15	38.455590959420476	40.0	38.0	41.0	34.0	41.0
16	38.538789799319055	40.0	38.0	41.0	35.0	41.0
17	38.508872198842084	40.0	38.0	41.0	35.0	41.0
18	38.47399053486529	40.0	37.0	41.0	35.0	41.0
19	38.503021461439516	40.0	37.0	41.0	35.0	41.0
20	38.450511147849596	40.0	37.0	41.0	34.0	41.0
21	38.365139301711544	40.0	37.0	41.0	34.0	41.0
22	38.343184294952295	40.0	37.0	41.0	34.0	41.0
23	38.30068291361918	40.0	37.0	41.0	34.0	41.0
24	38.28061481881059	40.0	37.0	41.0	34.0	41.0
25	38.1923667423033	40.0	37.0	41.0	34.0	41.0
26	38.10310969204461	40.0	37.0	41.0	34.0	41.0
27	38.104610049116495	40.0	36.0	41.0	34.0	41.0
28	38.05008178802379	40.0	36.0	41.0	34.0	41.0
29	38.011133654079245	40.0	36.0	41.0	34.0	41.0
30	37.97725877992797	40.0	36.0	41.0	34.0	41.0
31	37.896431683522934	40.0	36.0	41.0	34.0	41.0
32	37.80940442157631	40.0	36.0	41.0	34.0	41.0
33	37.746872072174384	40.0	36.0	41.0	33.0	41.0
34	37.69960973244724	40.0	36.0	41.0	33.0	41.0
35	37.64816954253305	40.0	36.0	41.0	33.0	41.0
36	37.563503104450625	40.0	35.0	41.0	33.0	41.0
37	37.51222015719898	40.0	35.0	41.0	33.0	41.0
38	37.4763710140623	40.0	35.0	41.0	33.0	41.0
39	37.455820271636696	40.0	35.0	41.0	33.0	41.0
40	37.3338261726044	39.0	35.0	41.0	33.0	41.0
41	37.22871818838981	39.0	35.0	41.0	33.0	41.0
42	37.20886411831637	39.0	35.0	41.0	33.0	41.0
43	37.10194347563066	39.0	35.0	41.0	33.0	41.0
44	36.98904105999026	39.0	35.0	41.0	32.0	41.0
45	36.98359871672516	39.0	35.0	41.0	32.0	41.0
46	36.90311231275565	39.0	35.0	41.0	32.0	41.0
47	36.841726524434854	39.0	35.0	41.0	32.0	41.0
48	36.80908556839947	39.0	35.0	41.0	32.0	41.0
49	36.81503239854026	39.0	35.0	41.0	32.0	41.0
50	36.72469648890238	39.0	35.0	41.0	32.0	41.0
51	35.788574136639504	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	12.0
10	17.0
11	18.0
12	11.0
13	3.0
14	14.0
15	11.0
16	21.0
17	18.0
18	60.0
19	83.0
20	180.0
21	297.0
22	473.0
23	713.0
24	1064.0
25	1602.0
26	2191.0
27	2682.0
28	3170.0
29	3957.0
30	5093.0
31	6513.0
32	8870.0
33	13307.0
34	27535.0
35	39985.0
36	32264.0
37	51385.0
38	97675.0
39	158583.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.97380817705524	4.453898416872137	55.23126682987872	17.341026576193897
2	18.829372055794938	4.76816534939538	54.01853279492281	22.38392979988687
3	20.8923084314826	4.92518961936356	51.41070691496448	22.77179503418936
4	19.453756461690663	4.673601359275461	51.272246014881276	24.600396164152606
5	18.352839431218346	6.21021160057743	49.360218916729096	26.076730051475135
6	22.14260599138223	6.596111301597979	51.79049162355233	19.47079108346746
7	82.803549316322	2.822724185450249	8.993843512975795	5.379882985251949
8	84.03637546927108	3.0723469122564104	7.153230790734039	5.738046827738479
9	78.49924982146406	5.586919157616114	9.842517105599367	6.071313915320458
10	41.92941114806799	25.661565743812393	16.580801981257547	15.828221126862069
11	29.972417016276797	23.382857492285282	25.47221937098567	21.172506120452248
12	26.858793905099688	21.3201395091845	30.409202190040862	21.411864395674954
13	23.83667728782614	23.92447110775272	31.728730199982092	20.510121404439047
14	19.859093102943714	26.19182294476196	31.4175207636752	22.531563188619124
15	17.29581931070932	24.761569893271542	36.87427793950962	21.06833285650952
16	19.916311960706807	24.053977911773764	32.72329004064286	23.30642008687657
17	20.810411211401842	23.63772164117661	29.368343120961104	26.183524026460447
18	21.789246785807105	23.482007726729723	31.19738103609811	23.531364451365064
19	24.03017311980362	24.300324749776696	29.47666584405459	22.192836286365093
20	25.391850899013086	24.953318584553966	29.151042497013478	20.50378801941947
21	25.198791852209368	25.484667748437946	29.11020308326654	20.20633731608614
22	22.523045877730727	23.538134621558406	29.285135545359047	24.65368395535182
23	22.441585442823726	24.598430630870666	28.13879285681527	24.821191069490336
24	22.233894092698918	22.920301993269142	30.15979785582158	24.68600605821036
25	20.545501003513937	25.106848573132034	28.775844032750154	25.57180639060388
26	19.5627343625448	28.745050677999785	28.08572345820293	23.60649150125248
27	20.100853696622124	27.24403842835959	29.467711747992425	23.187396127025863
28	17.453717151025025	25.602599745354244	33.162477532862624	23.781205570758107
29	18.61097946891291	24.566108528012126	31.515579035185233	25.30733296788974
30	21.98034029932888	24.497096470557402	30.132498782461326	23.39006444765239
31	21.872454361409158	25.815532517564222	28.466381737138313	23.845631383888303
32	22.396159784752268	25.897429737644984	26.082408258734063	25.62400221886868
33	22.553184054720447	24.54907390623533	27.447580319333643	25.450161719710586
34	19.799908711898684	24.62332738577522	30.142981626631666	25.433782275694433
35	19.757322157456684	24.521338047701306	28.24973629095134	27.471603503890663
36	22.28805545424566	24.946766806947505	28.91539689576777	23.849780843039063
37	19.76300036471562	24.598212238283786	32.046273021308565	23.59251437569203
38	19.72849433598826	25.38289680295092	28.044884044455998	26.843724816604826
39	22.025110779639697	23.10418855142381	28.789165980549956	26.081534688386537
40	23.304454553594635	22.85784171342088	29.598092122360995	24.239611610623488
41	18.671037430305464	23.37106429259365	29.148421785970896	28.80947649112999
42	20.578041498959358	23.311006331201092	27.742191919037502	28.368760250802044
43	20.943849081986762	22.518896418579967	29.430585008222483	27.106669491210788
44	20.621501623748884	22.676794258895676	29.28185965655582	27.419844460799624
45	20.583938098805174	23.72704420921136	28.033964415111896	27.655053276871573
46	21.599682020393498	23.59316955345268	30.48105335112505	24.32609507502877
47	19.0789947826011	22.33304432714336	32.54988632665853	26.038074563597014
48	18.94664887495059	22.10962871076304	31.49330299132326	27.450419422963108
49	20.771100545763073	20.633513216027396	32.66585279029289	25.92953344791664
50	20.622811979270175	21.25440334053301	30.139487345241555	27.983297334955264
51	19.600079494901625	21.76522360125008	27.575558375246512	31.05913852860179
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	201.5
2	340.0
3	811.0
4	1282.0
5	883.5
6	485.0
7	535.5
8	586.0
9	658.5
10	731.0
11	718.5
12	706.0
13	742.0
14	778.0
15	769.0
16	760.0
17	780.0
18	800.0
19	885.5
20	971.0
21	971.5
22	972.0
23	1444.0
24	1916.0
25	2099.0
26	2777.5
27	3273.0
28	3497.5
29	3722.0
30	4864.5
31	6007.0
32	7172.0
33	8337.0
34	9739.5
35	11142.0
36	12415.0
37	13688.0
38	14842.0
39	15996.0
40	19523.0
41	23050.0
42	25201.0
43	27352.0
44	30217.0
45	33082.0
46	38286.0
47	43490.0
48	49047.0
49	54604.0
50	52781.0
51	50958.0
52	42676.0
53	34394.0
54	29802.0
55	25210.0
56	23070.0
57	20930.0
58	19232.5
59	17535.0
60	16883.0
61	16231.0
62	14364.5
63	12498.0
64	10345.5
65	8193.0
66	6899.5
67	5606.0
68	4772.5
69	3939.0
70	3078.0
71	2217.0
72	1999.0
73	1781.0
74	1351.5
75	748.5
76	575.0
77	425.5
78	276.0
79	193.0
80	110.0
81	94.0
82	78.0
83	44.0
84	10.0
85	7.0
86	4.0
87	4.0
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.487028450709886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01158950609607	21.344453211517724
2	12.655580061224688	7.716620587735876
3	4.952470966081189	4.529583697326957
4	2.4244744030049628	2.9566008040972065
5	1.3712637914333474	2.0902879111428385
6	0.9264761737354115	1.6947303280545791
7	0.6209581067520495	1.3251817227074074
8	0.4643549125112747	1.1325441143166506
9	0.37427902984705586	1.0269589888306156
>10	5.781143639552643	44.45291302576331
>50	0.3547979982970998	6.414785597813704
>100	0.05613436889893263	2.845701310108552
>500	0.0035983569807008092	0.6542699542593449
>1k	0.0028786855845606475	1.8153687463252244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	2445	0.5339698749265656	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	2030	0.4433369513705227	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1957	0.4273942925281344	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1842	0.40227914503670087	TruSeq Adapter, Index 22 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTG	757	0.16532318826969736	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.14675981838472474	No Hit
GCCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTG	527	0.11509289328683027	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTG	519	0.11334575259177401	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	507	0.11072504154918965	No Hit
TGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTG	483	0.1054836194640209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45949800279979297	0.0
2	0.0	0.0	0.0	1.930153682863389	0.0
3	2.1839258688203088E-4	0.0	0.0	2.7454132096940103	0.0
4	2.1839258688203088E-4	0.0	0.0	3.6041328613141554	0.0
5	2.1839258688203088E-4	0.0	0.0	4.961442788785978	0.0
6	2.1839258688203088E-4	0.0	0.0	5.813829055386544	0.0
7	2.1839258688203088E-4	0.0	0.0	6.6144562788960695	0.0
8	2.1839258688203088E-4	0.0	0.0	7.908432356172102	0.0
9	4.3678517376406176E-4	0.0	0.0	8.515345355117265	0.0
10	4.3678517376406176E-4	0.0	0.0	9.683090517175485	0.0
11	4.3678517376406176E-4	0.0	0.0	11.727463522978177	0.0
12	4.3678517376406176E-4	0.0	0.0	13.721606233798	0.0
13	4.3678517376406176E-4	0.0	0.0	14.316070855290889	0.0
14	4.3678517376406176E-4	0.0	0.0	14.540141649431852	0.0
15	4.3678517376406176E-4	0.0	0.0	14.855282152302623	0.0
16	4.3678517376406176E-4	0.0	0.0	15.634943687471473	0.0
17	4.3678517376406176E-4	0.0	0.0	16.75507926558941	0.0
18	4.3678517376406176E-4	0.0	0.0	18.03922767645575	0.0
19	4.3678517376406176E-4	0.0	0.0	18.881567884059745	0.0
20	4.3678517376406176E-4	0.0	0.0	19.8385641997768	0.0
21	4.3678517376406176E-4	0.0	0.0	21.047148775581963	0.0
22	4.3678517376406176E-4	0.0	0.0	22.236951588915264	0.0
23	6.551777606460926E-4	0.0	0.0	23.33415594541059	0.0
24	6.551777606460926E-4	0.0	0.0	24.171909908690058	0.0
25	8.735703475281235E-4	0.0	0.0	24.933881644321467	0.0
26	8.735703475281235E-4	0.0	0.0	25.6043468860493	0.0
27	8.735703475281235E-4	0.0	0.0	26.24751305441688	0.0
28	8.735703475281235E-4	0.0	0.0	26.909679377843197	0.0
29	8.735703475281235E-4	0.0	0.0	27.63255884042272	0.0
30	8.735703475281235E-4	0.0	0.0	28.409381271962104	0.0
31	8.735703475281235E-4	0.0	0.0	29.169605866898454	0.0
32	8.735703475281235E-4	0.0	0.0	29.86365750800955	0.0
33	8.735703475281235E-4	0.0	0.0	30.494812084098616	0.0
34	8.735703475281235E-4	0.0	0.0	31.16418536289204	0.0
35	8.735703475281235E-4	0.0	0.0	31.840983989639454	0.0
36	8.735703475281235E-4	0.0	0.0	32.52913903090474	0.0
37	0.0010919629344101544	0.0	0.0	33.226029775645294	0.0
38	0.0010919629344101544	0.0	0.0	33.91178249845487	0.0
39	0.0010919629344101544	0.0	0.0	34.64885747918173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.7993824E-9	45.000004	1
ACGCCGA	20	7.0288347E-4	45.000004	42
ATTAGCC	20	7.0288347E-4	45.000004	22
CGTTGAA	20	7.0288347E-4	45.000004	18
CCGGACC	20	7.0288347E-4	45.000004	14
AAACGCA	30	2.1626292E-6	45.000004	15
TACGCTA	20	7.0288347E-4	45.000004	14
TCTACGG	30	2.1626292E-6	45.000004	2
TCTACGC	20	7.0288347E-4	45.000004	12
CGTAGGC	20	7.0288347E-4	45.000004	33
AGTTCGT	20	7.0288347E-4	45.000004	29
ATGGTAC	20	7.0288347E-4	45.000004	17
CTCGAGC	20	7.0288347E-4	45.000004	14
TGGATCG	20	7.0288347E-4	45.000004	34
CCCGATA	20	7.0288347E-4	45.000004	24
GCGAGGT	20	7.0288347E-4	45.000004	21
CGGAATG	35	1.2097917E-7	45.0	6
ACACGCG	25	3.8871167E-5	45.0	11
GCGACCG	25	3.8871167E-5	45.0	31
GACCGTT	25	3.8871167E-5	45.0	1
>>END_MODULE